Skip to main content

REGULATION OF TRANSCRIPTION

Objective

"During the first step of gene expression, RNA Polymerase (RNAP) transcribes RNA from a DNA template in every living cell. Crystal structures of RNAP core as well as RNAP elongation complexes (ECs) have revolutionized our understanding of transcription. This groundwork paved the way for more complex questions addressing the regulation of transcription. Because of its fundamental role, transcriptional regulation has an impact on every aspect of biology including a growing list of human diseases. Thus, a detailed and mechanistic understanding is crucial and this is the area where the work proposed here will contribute. A combination of biochemistry and structural approaches (primarily X-ray crystallography) will be used to gain detailed understanding on the regulation of transcription by protein factors and non-coding RNAs. To complement the structural and biochemical results, Fluorescence Resonance Energy Transfer (FRET) studies will be used to study the dynamics of RNAP during processes that regulate transcription such as transcriptional pausing. The FRET assay will also be used to test hypotheses regarding the recognition of errors made by RNA polymerase."

Field of science

  • /natural sciences/biological sciences/genetics and heredity/rna
  • /natural sciences/earth and related environmental sciences/geology/mineralogy/crystallography
  • /natural sciences/biological sciences/biochemistry

Call for proposal

FP7-PEOPLE-2013-CIG
See other projects for this call

Funding Scheme

MC-CIG - Support for training and career development of researcher (CIG)

Coordinator

CENTRE EUROPEEN DE RECHERCHE EN BIOLOGIE ET MEDECINE
Address
Rue Laurent Fries 1
67404 Illkirch Graffenstaden
France
Activity type
Research Organisations
EU contribution
€ 100 000
Administrative Contact
Steve Brooks (Dr.)