Objective Polyacrylamide gel electrophoresis (PAGE) is the most widespread tool to separate and detect macromolecules based on their molecular weight. New generation PAGE assays miniaturize the technique to obtain high throughput performance and reduce sample volume, reagents and cost. Here, we present a novel technology that will improve the range, the resolution and the accuracy that these new generation PAGE assays can resolve. On traditional PAGE assays, polyacrylamide gels with an acrylamide gradient are used to significantly improve the separation of macromolecules. However, on new generation PAGE assays the technology to fabricate those gradients at the millimetre scale with micron resolution is missing. The technology presented in this project, developed as a part of the ERC project GenesForceMotion, allows the fabrication of millimetre sized polyacrylamide gels with a well-defined and precise acrylamide microgradient. Moreover, the acrylamide density profile can be precisely controlled along the gel to improve the separation of macromolecules with similar molecular weights. This technology generates a market opportunity to improve the performance of the gel electrophoresis technique widely extended in worldwide life science laboratories. The expected outcome of the project is to test the benefits of our microgradient gel technology to improve the new generation miniaturized PAGE assays and make this technology available to the market Fields of science natural scienceschemical scienceselectrochemistryelectrophoresis Programme(s) FP7-IDEAS-ERC - Specific programme: "Ideas" implementing the Seventh Framework Programme of the European Community for research, technological development and demonstration activities (2007 to 2013) Topic(s) ERC-OA-2013-PoC - European Research Council ERC Proof of Concept Call for proposal ERC-2013-PoC See other projects for this call Funding Scheme CSA-SA(POC) - Supporting action (Proof of Concept) Host institution FUNDACIO INSTITUT DE BIOENGINYERIA DE CATALUNYA EU contribution € 148 962,65 Address CARRER BALDIRI REIXAC PLANTA 2A 10-12 08028 Barcelona Spain See on map Region Este Cataluña Barcelona Activity type Research Organisations Administrative Contact Josep Samitier (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data Beneficiaries (1) Sort alphabetically Sort by EU Contribution Expand all Collapse all FUNDACIO INSTITUT DE BIOENGINYERIA DE CATALUNYA Spain EU contribution € 148 962,65 Address CARRER BALDIRI REIXAC PLANTA 2A 10-12 08028 Barcelona See on map Region Este Cataluña Barcelona Activity type Research Organisations Administrative Contact Josep Samitier (Prof.) Links Contact the organisation Opens in new window Website Opens in new window Total cost No data
