Skip to main content

Exploiting native endowments by re-factoring, re-programming and implementing novel control loops in Pseudomonas putida for bespoke biocatalysis

Objective

We aim to engineer the lifestyle of Pseudomonas putida to generate a tailored, re-factored chassis with highly attractive new-to-nature properties, thereby opening the door to the production of thus far non-accessible compounds. This industrially driven project capitalises on the outstanding metabolic endowment and stress tolerance capabilities of this versatile bacterium for the production of specialty and bulk chemicals. Specifically, we will build streamlined P. putida strains with improved ATP availability utilizing this power on demand, decoupled from growth. The well-characterized, streamlined and re-factored strain platform will offer easy-to-use plug-in opportunities for novel, DNA-encoded functions under the control of orthogonal regulatory systems. To this end, we will deploy a concerted approach of genome refactoring, model-driven circuit design, implementation of ATP control loops, structured modelling and metabolic engineering. By drawing on a starkly improved, growth-uncoupled ATP-biosynthetic machinery, empowered P. putida strains will be able to produce a) n-butanol and isobutanol and their challenging gaseous derivatives 1-butene (BE) and (iso-)butadiene (BDE) using a novel, new-to-nature route starting from glucose, as well as b) new active ingredients for crop protection, such as tabtoxin, a high-value, ß-lactam-based secondary metabolite with a huge potential as a new herbicide. The game-changing innovations brought in – in particular the uncoupling of ATP-synthesis and production from growth - will provide strong versatility, enhanced efficiency and efficacy to the production processes, thereby overcoming current bottlenecks, matching market needs and fostering high-level research growth and development.

Field of science

  • /social sciences/economics and business/business and management/commerce
  • /engineering and technology/industrial biotechnology/bioprocessing technologies/biocatalysis
  • /natural sciences/biological sciences/genetics and heredity/genome
  • /social sciences/economics and business/economics/production economics
  • /engineering and technology/industrial biotechnology/metabolic engineering

Call for proposal

H2020-LEIT-BIO-2014-1
See other projects for this call

Funding Scheme

RIA - Research and Innovation action

Coordinator

WAGENINGEN UNIVERSITY
Address
Droevendaalsesteeg 4
6708 PB Wageningen
Netherlands
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 1 357 753,64

Participants (9)

UNIVERSITAET STUTTGART
Germany
EU contribution
€ 1 348 266,25
Address
Keplerstrasse 7
70174 Stuttgart
Activity type
Higher or Secondary Education Establishments
AGENCIA ESTATAL CONSEJO SUPERIOR DEINVESTIGACIONES CIENTIFICAS
Spain
EU contribution
€ 721 875
Address
Calle Serrano 117
28006 Madrid
Activity type
Research Organisations
EIDGENOESSISCHE TECHNISCHE HOCHSCHULE ZUERICH
Switzerland
EU contribution
€ 0
Address
Raemistrasse 101
8092 Zuerich
Activity type
Higher or Secondary Education Establishments
UNIVERSIDADE NOVA DE LISBOA
Portugal
EU contribution
€ 621 250
Address
Campus De Campolide
1099 085 Lisboa
Activity type
Higher or Secondary Education Establishments
INGENZA LIMITED
United Kingdom
EU contribution
€ 614 500
Address
Wallace Building Roslin Biocentre
EH25 9PP Roslin
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
LIFEGLIMMER GMBH
Germany
EU contribution
€ 581 750
Address
Markelstrasse 38
12163 Berlin
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
ABENGOA RESEARCH SL

Participation ended

Spain
EU contribution
€ 128 217,61
Address
Calle Energia Solar 1 - Campus Palmas Altas Sect Sunp Gu I Parcela Ze 3
41014 Sevilla
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
BASF SE
Germany
EU contribution
€ 592 212,50
Address
Carl Bosch Strasse 38
67063 Ludwigshafen Am Rhein
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
LUCITE INTERNATIONAL UK LIMITED
United Kingdom
EU contribution
€ 55 000
Address
Cassel Works New Road
TS23 1LE Billingham
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)