UNDERSTANDING THE CLOSTRIDIUM SPORE, A PREREQUISTE FOR DISEASE INTERVENTIONS AND EXPLOITATION

Deliverables

Other (20)

Sporulation Mutants Isolated

Directed & random from Clostridium sp

Germination Mutants Isolated

Directed & random from Clostridium sp

Increased understanding of disease

Improved epidemiology/ diagnostics

Characterised sporulation genes

Role of sporulation genes determined

ESRs recruited

Target date for all starters

First ESR scientific presentation

Given at national/international meeting

Appointment of Supervisory Board

Supervisory Board committee members selected

Conditionally CDEPTsporulating strain

Prototype strain for clinical trial

Decision-making at single cell level

Understanding sporulation/toxigenesis

Ethics

Delivery of ethics documents before commencement of the relevant part of the research

Exemplification of ACE technology

Demonstrated in target Clostridium sp

Characterised germination genes

Role of germination genes determined

Promotional material distributed

Web-downloads and meeting flyers

Mid-term Conference

Conference Proceedings at website

Submission of Doctorate Thesis

Subject to National requirements

Universal transposon exemplified

Exemplified in target Clostridium

Final Conference Proceedings

Conference Proceedings at website

Spore regulators common to toxins

Links to toxin/metabolite production

New sporulation/germination genes

In pathogenic & non-pathogenic clostridia

Asporogenous butanol producers

In C. acetobutylicum & Clostridium sp.

Documents, reports (1)

Final Report

Final Project report submitted to commission

Websites, patent fillings, videos etc. (2)

Social Media Established

Twitter account & Facebook Page active

CLOSPORE website

Website established at www.clospore.net

Publications

Peer reviewed articles (5)

Hijacking CRISPR-Cas for high-throughput bacterial metabolic engineering: advances and prospects

Author(s): Ioannis Mougiakos, Elleke F Bosma, Joyshree Ganguly, John van der Oost, Richard van Kranenburg
Published in: Current Opinion in Biotechnology, Issue 50, 2018, Page(s) 146-157, ISSN 0958-1669
DOI: 10.1016/j.copbio.2018.01.002

Investigating the Central Metabolism of Clostridium thermosuccinogenes

Author(s): Jeroen Girwar Koendjbiharie, Kilian Wiersma, Richard van Kranenburg
Published in: Applied and Environmental Microbiology, Issue 84/13, 2018, Page(s) e00363-18, ISSN 0099-2240
DOI: 10.1128/AEM.00363-18

L-Rhamnose metabolism in Clostridium beijerinckii DSM 6423

Author(s): M. Diallo, A. D. Simons, H. van der Wal, F. Collas, Bwee Houweling-Tan, S. W. M. Kengen, A. M. López-Contreras
Published in: Applied and Environmental Microbiology, 2018, ISSN 0099-2240
DOI: 10.1128/aem.02656-18

Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system

Author(s): Patrick Ingle, Daphne Groothuis, Peter Rowe, He Huang, Alan Cockayne, Sarah A. Kuehne, Weihong Jiang, Yang Gu, Christopher M. Humphreys, Nigel P. Minton
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
DOI: 10.1038/s41598-019-44458-y

RiboCas: A Universal CRISPR-Based Editing Tool for Clostridium

Author(s): Inés C. Cañadas, Daphne Groothuis, Maria Zygouropoulou, Raquel Rodrigues, Nigel P. Minton
Published in: ACS Synthetic Biology, 2019, ISSN 2161-5063
DOI: 10.1021/acssynbio.9b00075