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Self-amplified photonic biosensing platform for microRNA-based early diagnosis of diseases

Objective

The SAPHELY project focuses on the development and the preclinical validation of a nanophotonic-based handheld point-of-care (POC) analysis device for its application to the minimally-invasive early diagnosis of diseases, with a focus in cancer. Disease identification will be based in the fast (<5 minutes), ultra-sensitive (sub-pM) and label-free detection of novel highly-specific microRNA (miRNA) biomarkers, using a small volume of whole blood (<100 μL). This POC analysis device, which will have a low cost (envisaged cost < €3000), will significantly help in the implementation of mass screening programs, with the consequent impact on clinical management, reducing also costs of treatments, and increasing survival rates.
The ultra-high sensitivity required for the direct detection of miRNA biomarkers present in the bloodstream will be achieved by using a novel sensing amplification technique. This technique is based in the use of molecular beacon capture probes with an attached high index nanoparticle, so that the hybridization events are translated into the displacement of these nanoparticles from the sensor surface. The use of this self-amplification technique avoids the use of complex PCR-based amplification methods or labelling processes, which are difficult to implement on-chip.
The cost, size and weight reduction required for deploying an affordable handheld POC device will be achieved by using a novel power-based readout scheme for photonic bandgap sensing structures where the use of expensive, bulky and heavy tuneable lasers and spectrometers is avoided.
Special attention will be paid within the SAPHELY project to explore the potential deployment and commercialisation of the analysis device, by means of the involvement of relevant academic and industrial partners, as well as end users.

Call for proposal

H2020-ICT-2014-1
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Funding Scheme

RIA - Research and Innovation action

Coordinator

UNIVERSITAT POLITECNICA DE VALENCIA
Address
Camino De Vera Sn Edificio 3A
46022 Valencia
Spain
Activity type
Higher or Secondary Education Establishments
EU contribution
€ 945 704

Participants (8)

UNIVERSITY OF EAST ANGLIA
United Kingdom
EU contribution
€ 323 513
Address
Earlham Road
NR4 7TJ Norwich
Activity type
Higher or Secondary Education Establishments
AALBORG UNIVERSITET
Denmark
EU contribution
€ 344 915
Address
Fredrik Bajers Vej 7K
9220 Aalborg
Activity type
Higher or Secondary Education Establishments
MICROFLUIDIC CHIPSHOP GMBH
Germany
EU contribution
€ 443 750
Address
Stockholmer Strasse 20
07747 Jena
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
MICROTEC GESELLSCHAFT FUR MIKROTECHNOLOGIE MBH
Germany
EU contribution
€ 226 250
Address
Bismarckstrasse 142B
47057 Duisburg
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
APR TECHNOLOGIES AB
Sweden
EU contribution
€ 370 081
Address
Vaestra Jaernvaegsgatan 4
745 39 Enkoeping
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
EV GROUP E. THALLNER GMBH
Austria
EU contribution
€ 207 750
Address
Di Erich Thallner Strasse 1
4782 St Florian Am Inn
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
MEDICAL ENGINEERING TECHNOLOGIES LTD
United Kingdom
EU contribution
€ 151 250
Address
Unit 16 Holmestone Road
CT17 0UF Dover
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
Fundación Instituto Valenciano de Oncología
Spain
EU contribution
€ 215 625
Address
Beltrán Báguena 8
46009 Valencia
Activity type
Research Organisations