Harnessing Plant Reproduction for Crop Improvement

Deliverables

Documents, reports (4)

Genomic sequence annotation

Genomic sequence annotation

Delivery of candidate genes

Delivery of candidate genes

annotation of genes involved in apomixis

annotation of genes involved in apomixis

Identification of gene expression patterns distinguishing reproductive behaviours

Delivery of candidate genes

Other (9)

list of selected candidates suitable to be tested using reverse genetics approaches

CONICET will be the lead partner for this deliverable. A final list of selected candidates suitable to be tested using reverse genetics approaches

Organization of Workshop 1

Organization of the first Workshop

Delivery of DNA sample from 2x Paspalum to INDEAR

Delivery of DNA sample from 2x Paspalum to INDEAR

Hypotheses for ASR functional role

Hypotheses for ASR functional role and approach for validation through WP5

Mutant/transformants functional analysis

Phenotypic characterization of mutants/transformants

Organization of Workshop 2

Organization of the second workshop

Website & Depository of sequence

Project Website and repository for all the sequences produced by the project (available as WordPress Download Manager which is a Documents Management Plugin that will be used to manage, track, control file downloads. It includes password protection, limited viewing, and works on multiple domains).

Final Workshop

Organization of the final workshop

A list of candidates showing the same pattern of differential expression in aposporous and diplosporous plants

CONICET will be the lead partner for this deliverable. The lists of candidate genes differentially expressed in aposporous species (P. pratensis and P.notatum) will be compared to those originated from diplosporous species (E.curvula/T. dactyloides), in order to identify commonalities and differences between both reproductivetypes.

Publications

Peer reviewed articles (4)

The MAP3K-Coding QUI-GON JINN (QGJ) Gene Is Essential to the Formation of Unreduced Embryo Sacs in Paspalum

Author(s): Micaela Mancini, Hugo Permingeat, Carolina Colono, Lorena Siena, Fulvio Pupilli, Celeste Azzaro, Diva Maria de Alencar Dusi, Vera Tavares de Campos Carneiro, Maricel Podio, José Guillermo Seijo, Ana María González, Silvina A. Felitti, Juan Pablo A. Ortiz, Olivier Leblanc, Silvina C. Pessino
Published in: Frontiers in Plant Science, Issue 9, 2018, ISSN 1664-462X
DOI: 10.3389/fpls.2018.01547

De novo transcriptome sequencing and assembly from apomictic and sexual Eragrostis curvula genotypes

Author(s): Ingrid Garbus, José Rodolfo Romero, Juan Pablo Selva, María Cielo Pasten, Carolina Chinestra, José Carballo, Diego Carlos Zappacosta, Viviana Echenique
Published in: PLOS ONE, Issue 12/11, 2017, Page(s) e0185595, ISSN 1932-6203
DOI: 10.1371/journal.pone.0185595

A reference floral transcriptome of sexual and apomictic Paspalum notatum

Author(s): Juan Pablo A. Ortiz, Santiago Revale, Lorena A. Siena, Maricel Podio, Luciana Delgado, Juliana Stein, Olivier Leblanc, Silvina C. Pessino
Published in: BMC Genomics, Issue 18/1, 2017, ISSN 1471-2164
DOI: 10.1186/s12864-017-3700-z

Apomixis frequency under stress conditions in weeping lovegrass (Eragrostis curvula)

Author(s): Juan Manuel Rodrigo, Diego Carlos Zappacosta, Juan Pablo Selva, Ingrid Garbus, Emidio Albertini, Viviana Echenique
Published in: PLOS ONE, Issue 12/4, 2017, Page(s) e0175852, ISSN 1932-6203
DOI: 10.1371/journal.pone.0175852