Several aspects of GLYCANC reach beyond the current state of the art: Glycoscience is an under-investigated area of cancer research, and the mechanistic contributions we made to understand how specific GAG structural patterns and misexpression of PGs and GAGs contribute to tumor progression and metastasis is an important addition to the field. Several innovative techniques and subtopics with high commercialization potential for analytics, diagnostics and therapeutics resulted from GLYCANC. The application of quantitative AFM, RAMAN glycoanalytics, available as standardized protocols, cancer stem cell analysis and endocrine regulation of microRNAs targeting PGs and GAGs are highly novel aspects, which have been successfully evaluated in several cell models, in in vivo models of cancer, and in patient-derived samples. Epigenetic regulation of PG and GAG (mis)expression in cancer has been evaluated in mechanistic detail providing sufficient data in preclinical models to assess their potential for drug development. Overall our data strongly confirm that PGs and GAGs modulate all steps of tumor progression. Preclinical data for novel therapeutic approaches, including miRNA drugs, drugs inhibiting GAG-processing enzymes, and glycan-based inhibitors of cancer stem cells have been generated, which are laid down in 25 publications of our consortium, and were presented at major scientific conferences, notably the GRC and International conferences for proteoglycans, several FEBS lecture courses, and Matrix Biology Europe. Moreover, our research was disseminated via social media, open university days and press notices. We have strengthened the European research area by establishing a novel and strong network of European and non-European researchers benefitting considerably from the exchange of expertise and resources, thus strengthening their position as opinion leaders. Moreover, we have strengthened cooperation between academic and non-academic partners for their mutual benefit, and exploited the translational potential of this project in the fields of quantitative AFM, spectroscopy-based glycoanalytics, as well as miRNA- and PG/GAG-based anticancer therapeutics. By this way, the impact on society is high, as young European researchers received excellent interdisciplinary training in an important and specialized research area for which a high demand exists both in the academic community and in industry. By closing important knowledge gaps in the area of carbohydrate-related cancer research, GLYCANC made a major contribution to the preclinical development of more efficient anti-cancer drugs and diagnostics.