Matrix glycans as multifunctional pathogenesis factors and therapeutic targets in cancer

Deliverables

Documents, reports (45)

Methods for preprocessing and processing of spectral data

Presentation of a document describing methods for preprocessing and processing of spectral data.

Proof of concept to determine GAGs composition in cancer stromal tissue

Report on the experimental results on the proof of concept to determine GAGs composition in cancer stromal tissue.

Changes in invasive behaviour

Report on experimental results on changes in invasive behaviour of cancer cells subjected to PG/GAG manipulation.

Functional in vitro studies

Report on the experimental results of functional in vitro experiments after treatments of cancer cell lines stimulating or inhibiting HBP, O-GlcNAcylation and epigenetic modifications.

Analysis of angiogenic factors in immunomodulatory cells

Report on the experimental results of the analysis of angiogenic factors in immunomodulatory cells in the context of inflammatory breast cancer.

microRNA regulation of Glypican-1, Syndecan-2 and HAS1-3 in breast and colon cancer

Report on experimental results of microRNA regulation of Glypican-1, Syndecan-2 and HAS1-3 in breast and colon cancer.

Characterization of HS disaccharide structure from 8 breast cancer cell lines with altered Syndecan-1, 3-O-HS sulfation and 2-O-HS sulfation

Report on experimental results regarding the characterization of HS disaccharide structure from 8 breast cancer cell lines with altered Syndecan-1, 3-O-HS sulfation and 2-O-HS sulfation.

Correlation of GAG structure with clinicopathological parameters

Report on the correlation of GAG structure with clinicopathological parameters, including statistical analysis.

microRNA-regulation of Syndecan-1 in clinical IBC specimens

Report on experimental results of microRNA-regulation of Syndecan-1 in clinical IBC specimens.

Flow cytometric analysis of SP and ALDH phenotype

Report on the experimental results of flow cytometric analysis of SP and ALDH phenotype of cancer stem cells.

Chemotherapeutic resistance

Report on experimental results regarding chemotherapeutic resistance of PG/GAG-manipulated cancer stem cells.

In vivo study on the premetastatic niche

Report on the experimental results of the in vivo study on the premetastatic niche.

Diagnostic value of Syndecan-1 in IBC

Report on the diagnostic value of Syndecan-1 in IBC including statistical analysis.

ChIP experiments

Report on the experimental results of chromatin immunoprecipitation experiments.

Nanostic analysis

Report on the experimental results of the nanostic analysis of cancer cells.

Standardized protocol for FTIR and RAMAN spectroscopic analysis of GAGs in cells and conditioned media.

Presentation of a standardized experimental protocol for FTIR and RAMAN spectroscopic analysis of GAGs in cells and conditioned media.

qPCR analysis of angiogenic factors in tumors

Experimental data report on qPCR analysis of angiogenic factors in tumors related to syndecan-1.

Spectroscopic delineation of the expression of GAGs in mice lung metastatic nodules

Report on the experimental results of spectroscopic analysis of the expression of GAGs in mice lung metastatic nodules.

3D culture

Report on the experimental results of 3D cultures of cancer stem cell-enriched cultures.

Functional impact of miRNA-dependent PG and HAS regulation

Report on the experimental results of the functional impact of miRNA-dependent PG and HAS regulation.

Spectroscopic delineation of the expression of GAGs in murine melanoma, and PG-manuipulated cancer cell lines

Report on the experimental results of the spectroscopic analysis of the expression of GAGs in murine melanoma, and PG-manuipulated cancer cell lines.

Mechanistic in vitro investigation of Syndecan-dependenttranscriptional activation

Report on the experimental results of the mechanistic in vitro investigation of Syndecan-dependent transcriptional activation.

Characterization of HA molecular weight from tumor and control tissue of at least three in vivo mouse tumor models

Report on the experimental results of the characterization of HA molecular weight from tumor and control tissue of at least three in vivo mouse tumor models.

In vitro analysis of microRNA-dependent Syndecan-1 regulation in IBC

Report on experimental results of the in vitro analysis of microRNA-dependent Syndecan-1 regulation in IBC.

Standardized Protocol for Tissue Microarray Analysis by FTIR and RAMAN spectroscopy

Presentation of a standardized Protocol (Document) for Tissue Microarray Analysis by FTIR and RAMAN spectroscopy.

Analysis of signaling pathways

Report on experimental results of signal transduction analysis in cancer stem cells.

Immune cells in the microenvironment

Experimental data report on the analysis of immune cells in the tumor microenvironment.

Role of Syndecan-1 oligomerization in FAK activation

Report on the experimental results on the role of Syndecan-1 oligomerization in FAK activation.

Therapeutic potential of Ascidian GAGs

Report on experimental data regarding the therapeutic potential of Ascidian GAGs in malignant disease.

Impact of PG/HA on DNA damage response

Report on the experimental results of the impact of PG/HA on the DNA damage response.

Proof of concept for in vivo Raman probes for diagnosis of melanoma in patients and application to tumor-associated macrophages

Report on the experimental results delivering proof of concept for in vivo Raman probes for diagnosis of melanoma in patients and application to tumor-associated macrophages.

Angiogenesis regulators

Experimental data report on the analysis of hyaluronic acid-dependent angiogenesis regulators.

Impact of conventional therapeutics on the premetastatic niche

Report on experimental data of the impact of conventional therapeutics on the premetastatic niche.

In vivo model of hepatocarcinogenesis

Exytablishment of, and report on the experimental results of the in vivo model of hepatocarcinogenesis.

Inhibitory effect of 4- MU in vivo

Experimental data report on the inhibitory effect of 4- MU in vivo.

Evaluation of HA related enzyme expression

Experimental data report on the evaluation of Evaluation of HA related enzyme expression in mouse cancerous tissues.

Microvascular density

Experimental data report on the analysis of microvascular densitty in in vivo models.

Impact of PGs and GAGs on premature senescence of cancer cells

Report on experimental results of the studies on the impact of PGs and GAGs on premature senescence of cancer cells.

Epigenetic HAS2 promoter regulation

Report on the experimental results of epigenetic HAS2 promoter regulation.

Flow cytometric and qPCR analysis of stem cell markers

Report on experimental results of flow cytometric and qPCR analysis of stem cell markers.

In vitro functional assays in a novel 3D culture system

Establishment and report on experimental data of functional in vitro assays in a novel 3D culture system.

In vitro study of the premetastatic niche

Report on experimental results on the in vitro study of the premetastatic niche.

Changes in EMT marker expression

Report on the experimental results of changes in EMT marker expression in cancer cells subjected to PG/GAG manipulation.

Analysis of glioblastoma cell line GAG structure

Report on the experimental results of the analysis of glioblastoma cell line GAG structure.

Role of PGs in TGFbeta induced EMT

Report of experimental results on the role of PGs in TGFbeta induced EMT.

Websites, patent fillings, videos etc. (3)

Workshop on PGs and GAGs in metastasis

Website reporting and dissemination on the Workshop on PGs and GAGs in metastasis organized by WWU.

Workshop on epigenetic regulation

Website report and dissemination on the workshop epigenetic regulation of PG and GAG expression and function organized by partner UNINSUBRIA.

Establichment of GLYCANC Website

Establishment of GLYCANC website and social media presence (Facebook, Research Gate).

Publications

Peer reviewed articles (25)

Insights into the key roles of epigenetics in matrix macromolecules-associated wound healing

Author(s): Zoi Piperigkou, Martin Götte, Achilleas D. Theocharis, Nikos K. Karamanos
Published in: Advanced Drug Delivery Reviews, 2017, ISSN 0169-409X
DOI: 10.1016/j.addr.2017.10.008

Extracellular matrix alterations in senescent cells and their significance in tissue homeostasis

Author(s): Eleni Mavrogonatou, Harris Pratsinis, Adamantia Papadopoulou, Nikos K. Karamanos, Dimitris Kletsas
Published in: Matrix Biology, 2017, ISSN 0945-053X
DOI: 10.1016/j.matbio.2017.10.004

Changes in hyaluronan deposition in the rat myenteric plexus after experimentally-induced colitis

Author(s): Viviana Filpa, Michela Bistoletti, Ilaria Caon, Elisabetta Moro, Annalisa Grimaldi, Paola Moretto, Andreina Baj, Maria Cecilia Giron, Evgenia Karousou, Manuela Viola, Francesca Crema, Gianmario Frigo, Alberto Passi, Cristina Giaroni, Davide Vigetti
Published in: Scientific Reports, Issue 7/1, 2017, ISSN 2045-2322
DOI: 10.1038/s41598-017-18020-7

Syndecan-1 inhibits early stages of liver fibrogenesis by interfering with TGFβ1 action and upregulating MMP14

Author(s): Eszter Regős, Hadeer Hesham Abdelfattah, Andrea Reszegi, László Szilák, Klára Werling, Gábor Szabó, András Kiss, Zsuzsa Schaff, Ilona Kovalszky, Kornélia Baghy
Published in: Matrix Biology, 2018, ISSN 0945-053X
DOI: 10.1016/j.matbio.2018.02.008

Shed proteoglycans in tumor stroma

Author(s): Zoi Piperigkou, Benedikt Mohr, Nikos Karamanos, Martin Götte
Published in: Cell and Tissue Research, 2016, ISSN 0302-766X
DOI: 10.1007/s00441-016-2452-4

Extracellular matrix functions in lung cancer

Author(s): Martin Götte, Ilona Kovalszky
Published in: Matrix Biology, 2018, ISSN 0945-053X
DOI: 10.1016/j.matbio.2018.02.018

Tumor-suppressive functions of 4-MU on breast cancer cells of different ER status: Regulation of hyaluronan/HAS2/CD44 and specific matrix effectors

Author(s): Theodoros T. Karalis, Paraskevi Heldin, Demitrios H. Vynios, Thomas Neill, Simone Buraschi, Renato V. Iozzo, Nikos K. Karamanos, Spyros S. Skandalis
Published in: Matrix Biology, 2018, ISSN 0945-053X
DOI: 10.1016/j.matbio.2018.04.007

Co-treatment of tumor cells with hyaluronan plus doxorubicin affects endothelial cell behavior independently of VEGF expression

Author(s): Daiana L. Vitale, Fiorella M. Spinelli, Daiana Del Dago, Antonella Icardi, Gianina Demarchi, Ilaria Caon, Mariana García, Marcela F. Bolontrade, Alberto Passi, Carolina Cristina, Laura Alaniz
Published in: Oncotarget, Issue 9/93, 2018, ISSN 1949-2553
DOI: 10.18632/oncotarget.26379

microRNA miR-142-3p Inhibits Breast Cancer Cell Invasiveness by Synchronous Targeting of WASL, Integrin Alpha V, and Additional Cytoskeletal Elements

Author(s): Alexander Schwickert, Esther Weghake, Kathrin Brüggemann, Annika Engbers, Benjamin F. Brinkmann, Björn Kemper, Jochen Seggewiß, Christian Stock, Klaus Ebnet, Ludwig Kiesel, Christoph Riethmüller, Martin Götte
Published in: PLOS ONE, Issue 10/12, 2015, Page(s) e0143993, ISSN 1932-6203
DOI: 10.1371/journal.pone.0143993

Syndecan-1 in Liver Diseases

Author(s): Eszter Regős, Katalin Karászi, Andrea Reszegi, András Kiss, Zsuzsa Schaff, Kornélia Baghy, Ilona Kovalszky
Published in: Pathology & Oncology Research, 2019, ISSN 1219-4956
DOI: 10.1007/s12253-019-00617-0

IGF-IR cooperates with ERα to inhibit breast cancer cell aggressiveness by regulating the expression and localisation of ECM molecules

Author(s): Nikolaos A. Afratis, Panagiotis Bouris, Spyros S. Skandalis, Hinke A. Multhaupt, John R. Couchman, Achilleas D. Theocharis, Nikos K. Karamanos
Published in: Scientific Reports, Issue 7, 2017, Page(s) 40138, ISSN 2045-2322
DOI: 10.1038/srep40138

Implementation of infrared and Raman modalities for glycosaminoglycan characterization in complex systems

Author(s): Hossam Taha Mohamed, Valérie Untereiner, Ganesh D. Sockalingum, Stéphane Brézillon
Published in: Glycoconjugate Journal, 2016, ISSN 0282-0080
DOI: 10.1007/s10719-016-9743-6

Hyaluronan preconditioning of monocytes/macrophages affects their angiogenic behavior and regulation of TSG ‐6 expression in a tumor type‐specific manner

Author(s): Fiorella M. Spinelli, Daiana L. Vitale, Antonella Icardi, Ilaria Caon, Alejandra Brandone, Paula Giannoni, Virginia Saturno, Alberto Passi, Mariana García, Ina Sevic, Laura Alaniz
Published in: The FEBS Journal, 2019, ISSN 1742-464X
DOI: 10.1111/febs.14871

Proteoglycans and glycosaminoglycans as regulators of cancer stem cell function and therapeutic resistance

Author(s): Daiana Vitale, Sampath Kumar Katakam, Burkhard Greve, Bohee Jang, Eok-Soo Oh, Laura Alaniz, Martin Götte
Published in: The FEBS Journal, 2019, ISSN 1742-464X
DOI: 10.1111/febs.14967

Tumor-specific inhibitory action of decorin on different hepatoma cell lines

Author(s): Zsolt Horváth, Andrea Reszegi, László Szilák, Titanilla Dankó, Ilona Kovalszky, Kornélia Baghy
Published in: Cellular Signalling, 2019, Page(s) 109354, ISSN 0898-6568
DOI: 10.1016/j.cellsig.2019.109354

The immunomodulatory role of tumor Syndecan-1 (CD138) on ex vivo tumor microenvironmental CD4+ T cell polarization in inflammatory and non-inflammatory breast cancer patients

Author(s): Moshira Ezzat Saleh, Ramy Gadalla, Hebatallah Hassan, Ahmed Afifi, Martin Götte, Mohamed El-Shinawi, Mona Mostafa Mohamed, Sherif Abdelaziz Ibrahim
Published in: PLOS ONE, Issue 14/5, 2019, Page(s) e0217550, ISSN 1932-6203
DOI: 10.1371/journal.pone.0217550

Molecular size-dependent specificity of hyaluronan on functional properties, morphology and matrix composition of mammary cancer cells

Author(s): Anastasia-Gerasimoula Tavianatou, Zoi Piperigkou, Carlo Barbera, Riccardo Beninatto, Valentina Masola, Ilaria Caon, Maurizio Onisto, Marco Franchi, Devis Galesso, Nikos K. Karamanos
Published in: Matrix Biology Plus, 2019, Page(s) 100008, ISSN 2590-0285
DOI: 10.1016/j.mbplus.2019.100008

Characterization of inflammatory breast cancer: a vibrational microspectroscopy and imaging approach at the cellular and tissue level

Author(s): Hossam Taha Mohamed, Valérie Untereiner, Isabelle Proult, Sherif Abdelaziz Ibrahim, Martin Götte, Mohamed El-Shinawi, Mona Mostafa Mohamed, Ganesh D. Sockalingum, Stéphane Brézillon
Published in: The Analyst, Issue 143/24, 2018, Page(s) 6103-6112, ISSN 0003-2654
DOI: 10.1039/c8an01292j

Dissecting the role of hyaluronan synthases in the tumor microenvironment

Author(s): Alberto Passi, Davide Vigetti, Simone Buraschi, Renato V. Iozzo
Published in: The FEBS Journal, Issue 286/15, 2019, Page(s) 2937-2949, ISSN 1742-464X
DOI: 10.1111/febs.14847

The extracellular matrix as a multitasking player in disease

Author(s): Achilleas D. Theocharis, Dimitra Manou, Nikos K. Karamanos
Published in: The FEBS Journal, Issue 286/15, 2019, Page(s) 2830-2869, ISSN 1742-464X
DOI: 10.1111/febs.14818

Probing glycosaminoglycan spectral signatures in live cells and their conditioned media by Raman microspectroscopy

Author(s): S. Brézillon, V. Untereiner, H. T. Mohamed, J. Hodin, A. Chatron-Colliet, F.-X. Maquart, G. D. Sockalingum
Published in: The Analyst, Issue 142/8, 2017, Page(s) 1333-1341, ISSN 0003-2654
DOI: 10.1039/C6AN01951J

Roles and targeting of the HAS/hyaluronan/CD44 molecular system in cancer

Author(s): Evgenia Karousou, Suniti Misra, Shibnath Ghatak, Katalin Dobra, Martin Götte, Davide Vigetti, Alberto Passi, Nikos K. Karamanos, Spyros S. Skandalis
Published in: Matrix Biology, Issue 59, 2017, Page(s) 3-22, ISSN 0945-053X
DOI: 10.1016/j.matbio.2016.10.001

Syndecan-1 is a novel molecular marker for triple negative inflammatory breast cancer and modulates the cancer stem cell phenotype via the IL-6/STAT3, Notch and EGFR signaling pathways

Author(s): Sherif Abdelaziz Ibrahim, Ramy Gadalla, Eslam A. El-Ghonaimy, Omnia Samir, Hossam Taha Mohamed, Hebatallah Hassan, Burkhard Greve, Mohamed El-Shinawi, Mona Mostafa Mohamed, Martin Götte
Published in: Molecular Cancer, Issue 16/1, 2017, Page(s) 57, ISSN 1476-4598
DOI: 10.1186/s12943-017-0621-z

Estrogen receptor beta as epigenetic mediator of miR-10b and miR-145 in mammary cancer

Author(s): Zoi Piperigkou, Marco Franchi, Martin Götte, Nikos K. Karamanos
Published in: Matrix Biology, 2017, ISSN 0945-053X
DOI: 10.1016/j.matbio.2017.08.002

Inhibition of Heparanase in Pediatric Brain Tumor Cells Attenuates their Proliferation, Invasive Capacity, and In Vivo Tumor Growth

Author(s): Argyris Spyrou, Soumi Kundu, Lulu Haseeb, Di Yu, Tommie Olofsson, Keith Dredge, Edward Hammond, Uri Barash, Israel Vlodavsky, Karin Forsberg-Nilsson
Published in: Molecular Cancer Therapeutics, Issue 16/8, 2017, Page(s) 1705-1716, ISSN 1535-7163
DOI: 10.1158/1535-7163.MCT-16-0900