Structural and mechanistic insights into RING E3-mediated ubiquitination

Project Information

RINGE3

Grant agreement ID: 647849

DOI 10.3030/647849

Project closed

EC signature date 22 April 2015

Start date 1 May 2015

End date 30 April 2020

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 000 000,00

EU contribution € 2 000 000,00

Coordinated by

BEATSON INSTITUTE FOR CANCER RESEARCH LBG
United Kingdom

Publications

Peer reviewed articles (10)

A General Strategy for Discovery of Inhibitors and Activators of RING and U-box E3 Ligases with Ubiquitin Variants

Author(s): Mads Gabrielsen, Lori Buetow, Mark A. Nakasone, Syed Feroj Ahmed, Gary J. Sibbet, Brian O. Smith, Wei Zhang, Sachdev S. Sidhu, Danny T. Huang
Published in: Molecular Cell, Issue 68/2, 2017, Page(s) 456-470.e10, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2017.09.027

Structural analysis of MDM2 RING separates degradation from regulation of p53 transcription activity

Author(s): Koji Nomura, Marta Klejnot, Dominika Kowalczyk, Andreas K Hock, Gary J Sibbet, Karen H Vousden, Danny T Huang
Published in: Nature Structural & Molecular Biology, Issue 24/7, 2017, Page(s) 578-587, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/nsmb.3414

Structure specific recognition protein-1 (SSRP1) is an elongated homodimer that binds histones

Author(s): Gabriele Marcianò, Stefano Da Vela, Giancarlo Tria, Dmitri I. Svergun, Olwyn Byron, Danny T. Huang
Published in: Journal of Biological Chemistry, 2018, Page(s) jbc.RA117.000994, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.RA117.000994

Ubiquitination Accomplished: E1 and E2 Enzymes Were Not Necessary

Author(s): Mark A. Nakasone, Danny T. Huang
Published in: Molecular Cell, Issue 62/6, 2016, Page(s) 807-809, ISSN 1097-2765
Publisher: Cell Press
DOI: 10.1016/j.molcel.2016.06.001

Structure of the human histone chaperone FACT Spt16 N-terminal domain

Author(s): G. Marcianò, D. T. Huang
Published in: Acta Crystallographica Section F Structural Biology Communications, Issue 72/2, 2016, Page(s) 121-128, ISSN 2053-230X
Publisher: John Wiley and Sons Ltd
DOI: 10.1107/S2053230X15024565

Casitas B-lineage lymphoma linker helix mutations found in myeloproliferative neoplasms affect conformation

Author(s): Lori Buetow, Giancarlo Tria, Syed Feroj Ahmed, Andreas Hock, Hao Dou, Gary J. Sibbet, Dmitri I. Svergun, Danny T. Huang
Published in: BMC Biology, Issue 14/1, 2016, ISSN 1741-7007
Publisher: BioMed Central
DOI: 10.1186/s12915-016-0298-6

Structural insights into the catalysis and regulation of E3 ubiquitin ligases

Author(s): Lori Buetow, Danny T. Huang
Published in: Nature Reviews Molecular Cell Biology, Issue 17/10, 2016, Page(s) 626-642, ISSN 1471-0072
Publisher: Nature Publishing Group
DOI: 10.1038/nrm.2016.91

Identification and characterization of mutations in ubiquitin required for non-covalent dimer formation

Author(s): Mads Gabrielsen, Lori Buetow, Dominika Kowalczyk, Wei Zhang, Sachdev S.Sidhu and Danny T.Huang
Published in: Structure, Issue 27/9, 2019, Page(s) 1452-1459.e4, ISSN 0969-2126
Publisher: Cell Press
DOI: 10.1016/j.str.2019.06.008

Structural insights into non-covalent ubiquitin activation of the cIAP1-UbcH5B∼ubiquitin complex

Author(s): Amrita Patel, Gary J. Sibbet, Danny T. Huang
Published in: Journal of Biological Chemistry, Issue 294/4, 2019, Page(s) 1240-1249, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra118.006045

Structural basis for DNA damage-induced phosphoregulation of MDM2 RING domain

Author(s): Helge M. Magnussen, Syed F. Ahmed, Gary. J. Sibbet, Ventzislava A. Hristova, Koji Nomura, Andreas K. Hock, Lewis J. Archibald, Andrew G. Jamieson, David Fushman, Karen H. Vousden, Allan M. Weissman, Danny T. Huang
Published in: Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-15783-y

Book chapters (1)

Single-Turnover RING/U-Box E3-Mediated Lysine Discharge Assays

Author(s): Lori Buetow, Mads Gabrielsen, Danny T. Huang
Published in: The Ubiquitin Proteasome System - Methods and Protocols, Issue 1844, 2018, Page(s) 19-31, ISBN 978-1-4939-8705-4
Publisher: Springer New York
DOI: 10.1007/978-1-4939-8706-1_2