Future bio-pesticides for EU: unlocking the code of Saponin Structure-Activity Relationships for use in pest management

Informations projet

SSAR

N° de convention de subvention: 655559

DOI

10.3030/655559

Projet clôturé

Date de signature de la CE 5 Mars 2015

Date de début 1 Mars 2016

Date de fin 4 Mai 2018

Financé au titre de

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coût total

€ 212 194,80

Contribution de l’UE

€ 212 194,80

Coordonné par

KOBENHAVNS UNIVERSITET
Denmark

Periodic Reporting for period 1 - SSAR (Future bio-pesticides for EU: unlocking the code of Saponin Structure-Activity Relationships for use in pest management)

Période du rapport: 2016-03-01 au 2018-02-28

Résumé du contexte et des objectifs généraux du projet

This project aims to elucidate the relationship between saponin chemical structures and their biological activities and evolution. The new knowledge gained will have great industrial application potential as a generic platform for the production of bio-active compounds from plants and their use for bio-pesticides as an alternative to chemically produced pesticides. The new bio-pesticides will both help to meet society’s need for more sustainable food production and to contribute to the development of the knowledge based bio-economy. Identification of genes involved in saponin biosynthetic pathway will also open the possibility to engineer and breed crop with tailored saponin-based pest resistance capabilities. First, the genes involved in the biosynthetic pathway of saponins will be identified. Then desired or novel saponins will be produced in plant tissues through metabolic engineering with the identified genes. Finally, purified saponins will be used in bioassays with a range of pest species, to unlock the code of exactly which saponin chemical structures have a given specific biological activity.

Travail effectué depuis le début du projet jusqu’à la fin de la période considérée dans le rapport et principaux résultats atteints jusqu’à présent

During this project, we elucidated the biosynthetic pathway of insect-resistance associated saponins through identification of the key biosynthesis gene, CYP72A552. With all the biosynthetic genes at hand, we managed to produce desired saponins in tobacco plants through metabolic engineering. Later we showed that those tobacco plants gained resistance against its herbivore insects because of the accumulation of desired saponins. In the end, we managed to produce desired saponins in yeast, a potential production platform, which is a crucial step for commercial production of this compound in large scale.

Progrès au-delà de l’état des connaissances et impact potentiel prévu (y compris l’impact socio-économique et les conséquences sociétales plus larges du projet jusqu’à présent)

We have identified bio-active saponins and shown the possibility to produce them in a production platform. This will help to commercialize the production of bio-active saponins in industry scale, which will contribute to society’s need for more sustainable food production. We successfully enabled tobacco plants to gain resistance against its herbivore through transiently expressing the whole saponin biosynthesis pathway, which showed the possibility to engineer and breed crop with tailored saponin-based pest resistance capabilities.

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