Future bio-pesticides for EU: unlocking the code of Saponin Structure-Activity Relationships for use in pest management

Projektinformationen

SSAR

ID Finanzhilfevereinbarung: 655559

DOI

10.3030/655559

Projekt abgeschlossen

EK-Unterschriftsdatum 5 März 2015

Startdatum 1 März 2016

Enddatum 4 Mai 2018

Finanziert unter

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Gesamtkosten

€ 212 194,80

EU-Beitrag

€ 212 194,80

Koordiniert durch

KOBENHAVNS UNIVERSITET
Denmark

Periodic Reporting for period 1 - SSAR (Future bio-pesticides for EU: unlocking the code of Saponin Structure-Activity Relationships for use in pest management)

Berichtszeitraum: 2016-03-01 bis 2018-02-28

Zusammenfassung vom Kontext und den Gesamtzielen des Projekts

This project aims to elucidate the relationship between saponin chemical structures and their biological activities and evolution. The new knowledge gained will have great industrial application potential as a generic platform for the production of bio-active compounds from plants and their use for bio-pesticides as an alternative to chemically produced pesticides. The new bio-pesticides will both help to meet society’s need for more sustainable food production and to contribute to the development of the knowledge based bio-economy. Identification of genes involved in saponin biosynthetic pathway will also open the possibility to engineer and breed crop with tailored saponin-based pest resistance capabilities. First, the genes involved in the biosynthetic pathway of saponins will be identified. Then desired or novel saponins will be produced in plant tissues through metabolic engineering with the identified genes. Finally, purified saponins will be used in bioassays with a range of pest species, to unlock the code of exactly which saponin chemical structures have a given specific biological activity.

Arbeit, die ab Beginn des Projekts bis zum Ende des durch den Bericht erfassten Berichtszeitraums geleistet wurde, und die wichtigsten bis dahin erzielten Ergebnisse

During this project, we elucidated the biosynthetic pathway of insect-resistance associated saponins through identification of the key biosynthesis gene, CYP72A552. With all the biosynthetic genes at hand, we managed to produce desired saponins in tobacco plants through metabolic engineering. Later we showed that those tobacco plants gained resistance against its herbivore insects because of the accumulation of desired saponins. In the end, we managed to produce desired saponins in yeast, a potential production platform, which is a crucial step for commercial production of this compound in large scale.

Fortschritte, die über den aktuellen Stand der Technik hinausgehen und voraussichtliche potenzielle Auswirkungen (einschließlich der bis dato erzielten sozioökonomischen Auswirkungen und weiter gefassten gesellschaftlichen Auswirkungen des Projekts)

We have identified bio-active saponins and shown the possibility to produce them in a production platform. This will help to commercialize the production of bio-active saponins in industry scale, which will contribute to society’s need for more sustainable food production. We successfully enabled tobacco plants to gain resistance against its herbivore through transiently expressing the whole saponin biosynthesis pathway, which showed the possibility to engineer and breed crop with tailored saponin-based pest resistance capabilities.

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