Objective
This proposal aims to unravel to signalling pathways that underlie induced plant cell totipotency, by combining cell and molecular techniques to chart the changes that take place as differentiated pollen cells are induced to switch developmental pathways and form embryos in culture.
Immature male gametophytes can be induced to change their developmental fate from pollen to haploid embryo development when exposed to stress treatments in vitro. This process (microspore embryogenesis) is widely exploited in plant breeding to generate homozygous (doubled-haploid) lines in a single generation, and also provides a unique system to understand plant totipotency and early cell fate decisions. In this proposal, I aim to understand the molecular basis for this switch in developmental pathways in Brassica napus. I will combine cell tracking, cell sorting of fluorescently tagged cells and high-throughput transcriptome sequencing to chart the changes that take place as immature pollen are converted into haploid embryos, and will also determine the function of a selection of genes in the model plant, arabidopsis.
The project will advance my career by broadening my existing cell biology expertise, by providing training in a new field, molecular biology, and by allowing me to work with the plant model, arabidopsis. In addition, my network will be expanded to include a large group of internationally active scientists and seed companies. This novel combination of classical and state-of the-art skills, combined with the experience of working with top international groups and a plant breeding company, will place me in a solid position for a career in academia or industry.
Fields of science (EuroSciVoc)
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
CORDIS classifies projects with EuroSciVoc, a multilingual taxonomy of fields of science, through a semi-automatic process based on NLP techniques. See: The European Science Vocabulary.
- natural sciences biological sciences cell biology
- natural sciences biological sciences developmental biology
- medical and health sciences medical biotechnology cells technologies stem cells
- agricultural sciences agriculture, forestry, and fisheries agriculture agronomy plant breeding
- medical and health sciences clinical medicine embryology
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Programme(s)
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
Multi-annual funding programmes that define the EU’s priorities for research and innovation.
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H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions
MAIN PROGRAMME
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H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
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Topic(s)
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Calls for proposals are divided into topics. A topic defines a specific subject or area for which applicants can submit proposals. The description of a topic comprises its specific scope and the expected impact of the funded project.
Funding Scheme
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
Funding scheme (or “Type of Action”) inside a programme with common features. It specifies: the scope of what is funded; the reimbursement rate; specific evaluation criteria to qualify for funding; and the use of simplified forms of costs like lump sums.
MSCA-IF-EF-ST - Standard EF
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Call for proposal
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
Procedure for inviting applicants to submit project proposals, with the aim of receiving EU funding.
(opens in new window) H2020-MSCA-IF-2014
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Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.
6708 PB Wageningen
Netherlands
The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.