Objective Recently, the host lab found that a novel ubiquitin E3 ligase, SP1, regulates the translocon of the outer chloroplast membrane (TOC), which is responsible for importing nucleus-encoded proteins into plastids. SP1 achieves this by promoting the degradation TOC components by the cytosolic 26S proteasome. This is the first time the ubiquitin proteasome system (UPS) has been shown to directly regulate plastid development and function. By increasing or decreasing SP1 levels in transgenic Arabidopsis plants, multiple effects of SP1 were observed; e.g. inefficient de-etiolation leading to low survival rates and delayed leaf senescence in sp1 null mutants (Science 2012, 338:655-9). Also, sp1 mutants are significantly more sensitive to stress (unpublished). These data suggest that SP1 is important for the differentiation and interconversion of different plastid types, and all of the effects are highly correlated with plant growth and yield. Thus, discovery of SP1 strongly suggests potential applications in agriculture, such as delaying leaf senescence to produce a staygreen phenotype, or controlling amyloplast development during grain development, by modifying SP1 expression. This project will investigate the function of SP1 in cereals, and assess the potential of SP1 for manipulating plastids to improve cereal yield. I will generate transgenic plants with manipulated SP1 expression in Brachypodium distachyon (a versatile cereal model) and Oryza sativa (rice). I will study the development of plastids in the transformants, and assess the transformants for beneficial effects on yield parameters, such as starch content, seed weight, germination, de-etiolation, leaf senescence and stress resistance. At the end, I will have thoroughly assessed the potential use of SP1 for manipulating cereal traits linked to yield. A major strength of the proposal is the complementarity of my expertise with that of the host. Our unique combination of skills will ensure success of the project. Fields of science natural sciencesbiological sciencesgeneticsnatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsagricultural sciencesagriculture, forestry, and fisheriesagricultureagronomyplant breedingnatural sciencesearth and related environmental sciencesatmospheric sciencesclimatologyclimatic changesagricultural sciencesagriculture, forestry, and fisheriesagriculturegrains and oilseedscereals Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2014-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF) Call for proposal H2020-MSCA-IF-2014 See other projects for this call Funding Scheme MSCA-IF-EF-ST - Standard EF Coordinator THE CHANCELLOR, MASTERS AND SCHOLARS OF THE UNIVERSITY OF OXFORD Net EU contribution € 195 454,80 Address Wellington square university offices OX1 2JD Oxford United Kingdom See on map Region South East (England) Berkshire, Buckinghamshire and Oxfordshire Oxfordshire Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Other funding € 0,00
