Objective
Human induced pluripotent stem cells (hiPSCs) have the potential to revolutionize biomedical sciences, however the standardised mass production, indispensable for their industrial and therapeutic use, is still a major challenge. Impeller stirred-tank bioreactors, currently used for hiPSC bioprocessing, impose detrimental stresses on cultured hiPSCs, resulting in heterogeneity of cells and cell-aggregates, limited bioprocess reproducibility, and low hiPSC expansion efficiency at high costs. The ambitious purpose of the POSEIDON project is the development of a next-generation automated bioreactor platform to significantly improve reproducibility, efficiency and cost-effectiveness of scalable hiPSC expansion. To demonstrate the utility of the method for the production of valuable human cell types, a novel process for hiPSC-cardiomyogenic differentiation will also be established. This pioneering bioreactor will be developed by advanced engineering design methods and will enable controlled hiPSC suspension culture by non-impeller-dependent low-shear hydrodynamic conditions. Purposely designed automated systems will lead to bioprocess standardisation with enhanced reproducibility, efficiency and scalability beyond the state in the art. The proposed Fellowship will enable a highly interdisciplinary collaboration between the Researcher, a talented post-doc with a significant track record in bioengineering, and the supervisor, a world leader in stem cell bioprocessing, whose group is embedded in one of Europe’s leading hubs for regenerative biomedicine. These working conditions will effectively promote the Researcher’s professional development, providing her with excellent new expertise in stem cell bioprocessing and fundamental leadership skills, significantly widening her career perspectives. The project outcomes will be of tremendous benefit to the European biomedical and pharmaceutical sectors, contributing to the European excellence, competitiveness and quality of life.
Fields of science
- engineering and technologyenvironmental biotechnologybioremediationbioreactors
- engineering and technologyelectrical engineering, electronic engineering, information engineeringelectronic engineeringcontrol systems
- medical and health sciencesbasic medicinepharmacology and pharmacypharmaceutical drugs
- engineering and technologyindustrial biotechnologybioprocessing technologies
- medical and health sciencesmedical biotechnologycells technologiesstem cells
Keywords
Programme(s)
Funding Scheme
MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)Coordinator
30625 Hannover
Germany