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Super-resolution visualisation and manipulation of metaphase chromosomes

Objectif

CHROMAVISION aims to develop a pioneering chromosome imaging and manipulation platform that will fuel the next decades of structural chromosome research. Chromosomal abnormalities are characteristic of many disorders such as cancer, impaired fertility due to maternal aging, and neurological disorders such as fragile X syndrome. If humanity is to fully understand the wide range of diseases that are associated to errors in cell division, we must be able to further 'zoom in' on healthy and diseased chromosomes in all their complexity. The CHROMAVISION platform will allow molecular biologists to automatically isolate individual chromosomes from small tissue or cell samples and have these delivered to a super-resolution microscope. Chromosome isolation and delivery is achieved by an opto-fluidic chip that is able to trap, visualise and lyse individual cells and separate metaphase chromosomes from cell lysate. Single chromosomes can be “hand-selected” and brought into focus of the Super-Resolution Correlative Tweezers Fluorescence Microscope (CTFM-SR3D) that is developed in CHROMAVISION. This instrument will for the first time enable 3D, super-resolution, real-time metaphase chromosome observation and manipulation studies under near-physiological conditions. The technique will push the boundaries of what is currently possible in microfluidics and super-resolution microscopy and combine these into a single powerful approach for chromosome studies. Furthermore, the platform will be applied in CHROMAVISION to address key challenges in clinical and fundamental chromosome research, potentially resulting in breakthrough discoveries. Better imaging and understanding of the chromosomal mechanisms will contribute to our knowledge of the etiology of human diseases and aid drug discovery. The platform will also have large clinical value, allowing identification and monitoring of e.g. cancer heterogeneity.

Appel à propositions

H2020-FETOPEN-2014-2015-RIA
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Coordinateur

STICHTING VU
Adresse
De Boelelaan 1105
1081 HV Amsterdam
Pays-Bas

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Type d’activité
Higher or Secondary Education Establishments
Contribution de l’UE
€ 1 054 003,75

Participants (4)

DANMARKS TEKNISKE UNIVERSITET
Danemark
Contribution de l’UE
€ 716 468,75
Adresse
Anker Engelundsvej 1 Bygning 101 A
2800 Kgs Lyngby

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Type d’activité
Higher or Secondary Education Establishments
KOBENHAVNS UNIVERSITET
Danemark
Contribution de l’UE
€ 747 320
Adresse
Norregade 10
1165 Kobenhavn

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Type d’activité
Higher or Secondary Education Establishments
UNIVERSITY COLLEGE LONDON
United Kingdom
Contribution de l’UE
€ 111 732,50
Adresse
Gower Street
WC1E 6BT London

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Type d’activité
Higher or Secondary Education Establishments
LUMICKS BV
Pays-Bas
Contribution de l’UE
€ 937 500
Adresse
Pilotenstraat 41
1059 CH Amsterdam

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Type d’activité
Private for-profit entities (excluding Higher or Secondary Education Establishments)