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MASS Spectrometry TRaining network for Protein Lipid adduct ANalysis

Deliverables

Network Summer School 1

Summer School Training Event

ESR Recruitment

All ESRs to have been recruited by partners by month12

Network Summer School 2

Summer School Training Event

Database of (lip)oxPTM Fragmentation

Creation of a Database of (lip)oxPTM Fragmentation

Mutant Protein Constructs

Production of mutant protein contructs

Joint Public Engagement Event

Organisation of a joint public engagement event

Database of oxPL/NO2PL Fragmentation

The creation of a Database of oxPL/NO2PL Fragmentation

Database of (lip)oxidation targets

Production of a database of (lip)oxidation targets

Open Conference

Organisation of an open conference

Data Management Plan Agreed

Production of a Data Management Plan

Special Issue on Lipoxidation Targets

Production of an article: Special Issue on Lipoxidation Targets

1st publication All ESRs

Production of 1st publication by all ESRs

Special Issue on Novel MS Methodology

Production of an article: Special Issue on Novel MS Methodology

Joint Training Certificates Awarded

Joint Training Certificates Awarded to ESR's

All ESR projects completed and reports submitted

All ESR practical work completed, reports submitted and draft theses prepared

Final Technical Training Reports

Production of Technical Training Reports

Technical Training Reports

Prodction of Technical Training Reports

Annual Management Reports

Submission of annual management reports

Project Website

MASSTRPLAN project website created and live

Bioinformatics Tool for oxPTMs

Creation of a Bioinformatics Tool for oxPTMs

Set of LC-MS Protocols for ox biomols

Creation of a set of LC-MS Protocols for ox biomols

Bioinformatics Tool for oxPLs

Creation of a Bioinformatics Tool for oxPLs

Protocols for novel chemical labeling

Creation of Protocols for novel chemical labeling

Searching for OpenAIRE data...

Publications

Drawbacks of Dialysis Procedures for Removal of EDTA

Author(s): Andreia Mónico, Eva Martínez-Senra, F. Javier Cañada, Silvia Zorrilla, Dolores Pérez-Sala
Published in: PLOS ONE, Issue 12/1, 2017, Page(s) e0169843, ISSN 1932-6203
DOI: 10.1371/journal.pone.0169843

Electrochemical oxidation of phosphatidylethanolamines studied by mass spectrometry

Author(s): Simone Colombo, Giulia Coliva, Agnieszka Kraj, Jean-Pierre Chervet, Maria Fedorova, Pedro Domingues, M. Rosário Domingues
Published in: Journal of Mass Spectrometry, 2017, ISSN 1076-5174
DOI: 10.1002/jms.4056

Proteomics in cardiovascular diseases: Unveiling sex and gender differences in the era of precision medicine

Author(s): Roberta Baetta, Marta Pontremoli, Alma Martinez Fernandez, Corinne M. Spickett, Cristina Banfi
Published in: Journal of Proteomics, Issue 173, 2018, Page(s) 62-76, ISSN 1874-3919
DOI: 10.1016/j.jprot.2017.11.012

The cysteine residue of glial fibrillary acidic protein is a critical target for lipoxidation and required for efficient network organization

Author(s): Álvaro Viedma-Poyatos, Yolanda de Pablo, Milos Pekny, Dolores Pérez-Sala
Published in: Free Radical Biology and Medicine, Issue 120, 2018, Page(s) 380-394, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2018.04.007

Chemistry and analysis of HNE and other prominent carbonyl-containing lipid oxidation compounds

Author(s): Bebiana C. Sousa, Andrew R. Pitt, Corinne M. Spickett
Published in: Free Radical Biology and Medicine, Issue 111, 2017, Page(s) 294-308, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2017.02.003

A Novel Lipidomics Workflow for Improved Human Plasma Identification and Quantification Using RPLC-MSn Methods and Isotope Dilution Strategies

Author(s): Evelyn Rampler, Angela Criscuolo, Martin Zeller, Yasin El Abiead, Harald Schoeny, Gerrit Hermann, Elena Sokol, Ken Cook, David A. Peake, Bernard Delanghe, Gunda Koellensperger
Published in: Analytical Chemistry, Issue 90/11, 2018, Page(s) 6494-6501, ISSN 0003-2700
DOI: 10.1021/acs.analchem.7b05382

Phospholipidome of endothelial cells shows a different adaptation response upon oxidative, glycative and lipoxidative stress

Author(s): Simone Colombo, Tânia Melo, Marta Martínez-López, M. Jesús Carrasco, M. Rosário Domingues, Dolores Pérez-Sala, Pedro Domingues
Published in: Scientific Reports, Issue 8/1, 2018, ISSN 2045-2322
DOI: 10.1038/s41598-018-30695-0

PARP1 promoter links cell cycle progression with adaptation to oxidative environment

Author(s): Julita Pietrzak, Corinne M. Spickett, Tomasz Płoszaj, László Virág, Agnieszka Robaszkiewicz
Published in: Redox Biology, Issue 18, 2018, Page(s) 1-5, ISSN 2213-2317
DOI: 10.1016/j.redox.2018.05.017

Impact of inhibition of the autophagy-lysosomal pathway on biomolecules carbonylation and proteome regulation in rat cardiac cells

Author(s): Giulia Coliva, Sofia Duarte, Dolores Pérez-Sala, Maria Fedorova
Published in: Redox Biology, 2019, Page(s) 101123, ISSN 2213-2317
DOI: 10.1016/j.redox.2019.101123

Comprehensive and Empirical Evaluation of Machine Learning Algorithms for Small Molecule LC Retention Time Prediction

Author(s): Robbin Bouwmeester, Lennart Martens, Sven Degroeve
Published in: Analytical Chemistry, Issue 91/5, 2019, Page(s) 3694-3703, ISSN 0003-2700
DOI: 10.1021/acs.analchem.8b05820

Integrated approaches to unravel the impact of protein lipoxidation on macromolecular interactions

Author(s): Silvia Zorrilla, Andreia Mónico, Sofia Duarte, Germán Rivas, Dolores Pérez-Sala, María A. Pajares
Published in: Free Radical Biology and Medicine, 2019, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2019.04.011

Modulation of the inflammatory response of immune cells in human peripheral blood by oxidized arachidonoyl aminophospholipids

Author(s): Simone Colombo, Carmen Martín-Sierra, Tânia Melo, Paula Laranjeira, Artur Paiva, Pedro Domingues, M. Rosário Domingues
Published in: Archives of Biochemistry and Biophysics, Issue 660, 2018, Page(s) 64-71, ISSN 0003-9861
DOI: 10.1016/j.abb.2018.10.003

Pro-oxidant and pro-inflammatory effects of glycated albumin on cardiomyocytes

Author(s): Alma Martinez Fernandez, Luca Regazzoni, Maura Brioschi, Erica Gianazza, Piergiuseppe Agostoni, Giancarlo Aldini, Cristina Banfi
Published in: Free Radical Biology and Medicine, 2019, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2019.06.023

Advanced lipoxidation end products (ALEs) as RAGE binders: Mass spectrometric and computational studies to explain the reasons why

Author(s): Marco Mol, Genny Degani, Crescenzo Coppa, Giovanna Baron, Laura Popolo, Marina Carini, Giancarlo Aldini, Giulio Vistoli, Alessandra Altomare
Published in: Redox Biology, 2018, Page(s) 101083, ISSN 2213-2317
DOI: 10.1016/j.redox.2018.101083

Vimentin filaments interact with the actin cortex in mitosis allowing normal cell division

Author(s): Sofia Duarte, Álvaro Viedma-Poyatos, Elena Navarro-Carrasco, Alma E. Martínez, María A. Pajares, Dolores Pérez-Sala
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
DOI: 10.1038/s41467-019-12029-4

A comparison of collision cross section values obtained via travelling wave ion mobility-mass spectrometry and ultra high performance liquid chromatography-ion mobility-mass spectrometry: Application to the characterisation of metabolites in rat urine

Author(s): Leanne C. Nye, Jonathan P. Williams, Nyasha C. Munjoma, Marine P.M. Letertre, Muireann Coen, Robbin Bouwmeester, Lennart Martens, Jonathan R. Swann, Jeremy K. Nicholson, Robert S. Plumb, Michael McCullagh, Lee A. Gethings, Steven Lai, James I. Langridge, Johannes P.C. Vissers, Ian D. Wilson
Published in: Journal of Chromatography A, Issue 1602, 2019, Page(s) 386-396, ISSN 0021-9673
DOI: 10.1016/j.chroma.2019.06.056

Accurate peptide fragmentation predictions allow data driven approaches to replace and improve upon proteomics search engine scoring functions

Author(s): Ana S C Silva, Robbin Bouwmeester, Lennart Martens, Sven Degroeve
Published in: Bioinformatics, 2019, ISSN 1367-4803
DOI: 10.1093/bioinformatics/btz383

Enzymatic and non-enzymatic detoxification of 4-hydroxynonenal: Methodological aspects and biological consequences

Author(s): Marco Mol, Luca Regazzoni, Alessandra Altomare, Genny Degani, Marina Carini, Giulio Vistoli, Giancarlo Aldini
Published in: Free Radical Biology and Medicine, 2017, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2017.01.036

Exercise training counteracts urothelial carcinoma-induced alterations in skeletal muscle mitochondria phospholipidome in an animal model

Author(s): Javier-Fernando Montero-Bullon, Tânia Melo, Rita Ferreira, Ana Isabel Padrão, Paula A. Oliveira, M. Rosário M. Domingues, Pedro Domingues
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
DOI: 10.1038/s41598-019-49010-6

Lipoproteins as targets and markers of lipoxidation

Author(s): Catarina B. Afonso, Corinne M. Spickett
Published in: Redox Biology, Issue 23, 2019, Page(s) 101066, ISSN 2213-2317
DOI: 10.1016/j.redox.2018.101066

Analysis of oxidised and glycated aminophospholipids: Complete structural characterisation by C30 liquid chromatography-high resolution tandem mass spectrometry

Author(s): Simone Colombo, Angela Criscuolo, Martin Zeller, Maria Fedorova, M. Rosário Domingues, Pedro Domingues
Published in: Free Radical Biology and Medicine, Issue 144, 2019, Page(s) 144-155, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2019.05.025

Lipoxidation and cancer immunity

Author(s): C. Martín-Sierra, P. Laranjeira, M.R. Domingues, A. Paiva
Published in: Redox Biology, Issue 23, 2019, Page(s) 101103, ISSN 2213-2317
DOI: 10.1016/j.redox.2019.101103

Insight into the cellular effects of nitrated phospholipids: Evidence for pleiotropic mechanisms of action

Author(s): Sofia Duarte, Tânia Melo, Rosário Domingues, Juan de Dios Alché, Dolores Pérez-Sala
Published in: Free Radical Biology and Medicine, Issue 144, 2019, Page(s) 192-202, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2019.06.003

Liquid chromatography/tandem mass spectrometry characterization of nitroso, nitrated and nitroxidized cardiolipin products

Author(s): Javier-Fernando Montero-Bullon, Tânia Melo, M. Rosário M Domingues, Pedro Domingues
Published in: Free Radical Biology and Medicine, Issue 144, 2019, Page(s) 183-191, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2019.05.009

Computational solutions in redox lipidomics – Current strategies and future perspectives

Author(s): Zhixu Ni, Laura Goracci, Gabriele Cruciani, Maria Fedorova
Published in: Free Radical Biology and Medicine, Issue 144, 2019, Page(s) 110-123, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2019.04.027

Functional and Phenotypic Characterization of Tumor-Infiltrating Leukocyte Subsets and Their Contribution to the Pathogenesis of Hepatocellular Carcinoma and Cholangiocarcinoma

Author(s): C. Martín-Sierra, R. Martins, P. Laranjeira, M. Coucelo, A.M. Abrantes, R.C. Oliveira, J.G. Tralhão, M.F. Botelho, E. Furtado, M.R. Domingues, A. Paiva
Published in: Translational Oncology, Issue 12/11, 2019, Page(s) 1468-1479, ISSN 1936-5233
DOI: 10.1016/j.tranon.2019.07.019

Lipoxidation in cardiovascular diseases

Author(s): Erica Gianazza, Maura Brioschi, Alma Martinez Fernandez, Cristina Banfi
Published in: Redox Biology, Issue 23, 2019, Page(s) 101119, ISSN 2213-2317
DOI: 10.1016/j.redox.2019.101119

Discovery of bioactive nitrated lipids and nitro-lipid-protein adducts using mass spectrometry-based approaches

Author(s): Tânia Melo, Javier-Fernando Montero-Bullón, Pedro Domingues, M. Rosário Domingues
Published in: Redox Biology, Issue 23, 2019, Page(s) 101106, ISSN 2213-2317
DOI: 10.1016/j.redox.2019.101106

Evaluation of air oxidized PAPC: A multi laboratory study by LC-MS/MS

Author(s): Zhixu Ni, Bebiana C. Sousa, Simone Colombo, Catarina B. Afonso, Tania Melo, Andrew R. Pitt, Corinne M. Spickett, Pedro Domingues, M. Rosário Domingues, Maria Fedorova, Angela Criscuolo
Published in: Free Radical Biology and Medicine, Issue 144, 2019, Page(s) 156-166, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2019.06.013

Lipoxidation targets: From basic mechanisms to pathophysiology

Author(s): Dolores Pérez-Sala, Rosário Domingues
Published in: Redox Biology, Issue 23, 2019, Page(s) 101208, ISSN 2213-2317
DOI: 10.1016/j.redox.2019.101208

Epitope mapping and characterization of 4-hydroxy-2-nonenal modified-human serum albumin using two different polyclonal antibodies

Author(s): Isabel Campos-Pinto, Lucía Méndez, James Schouten, John Wilkins, Maria Fedorova, Andrew R. Pitt, Paul Davis, Corinne M. Spickett
Published in: Free Radical Biology and Medicine, Issue 144, 2019, Page(s) 234-244, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2019.05.008

Cracking Proteoform Complexity of Ovalbumin with Anion-Exchange Chromatography–High-Resolution Mass Spectrometry under Native Conditions

Author(s): Florian Füssl, Angela Criscuolo, Ken Cook, Kai Scheffler, Jonathan Bones
Published in: Journal of Proteome Research, Issue 18/10, 2019, Page(s) 3689-3702, ISSN 1535-3893
DOI: 10.1021/acs.jproteome.9b00375

Short-chain lipid peroxidation products form covalent adducts with pyruvate kinase and inhibit its activity in vitro and in breast cancer cells

Author(s): Bebiana C. Sousa, Tanzim Ahmed, William L. Dann, Jed Ashman, Alexandre Guy, Thierry Durand, Andrew R. Pitt, Corinne M. Spickett
Published in: Free Radical Biology and Medicine, Issue 144, 2019, Page(s) 223-233, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2019.05.028

Vimentin disruption by lipoxidation and electrophiles: Role of the cysteine residue and filament dynamics

Author(s): Andreia Mónico, Sofia Duarte, María A. Pajares, Dolores Pérez-Sala
Published in: Redox Biology, Issue 23, 2019, Page(s) 101098, ISSN 2213-2317
DOI: 10.1016/j.redox.2019.101098

A mass spectrometry approach for the identification and localization of small aldehyde modifications of proteins

Author(s): Catarina B. Afonso, Bebiana C. Sousa, Andrew R. Pitt, Corinne M. Spickett
Published in: Archives of Biochemistry and Biophysics, Issue 646, 2018, Page(s) 38-45, ISSN 0003-9861
DOI: 10.1016/j.abb.2018.03.026

Rational selection of reverse phase columns for high throughput LC–MS lipidomics

Author(s): Angela Criscuolo, Martin Zeller, Ken Cook, Georgia Angelidou, Maria Fedorova
Published in: Chemistry and Physics of Lipids, Issue 221, 2019, Page(s) 120-127, ISSN 0009-3084
DOI: 10.1016/j.chemphyslip.2019.03.006

Liquid Chromatography Techniques in Lipidomics Research

Author(s): Mike Lange, Zhixu Ni, Angela Criscuolo, Maria Fedorova
Published in: Chromatographia, Issue 82/1, 2019, Page(s) 77-100, ISSN 0009-5893
DOI: 10.1007/s10337-018-3656-4

Oxidized phosphatidylserine mitigates LPS-triggered macrophage inflammatory status through modulation of JNK and NF-kB signaling cascades

Author(s): Elisabete Maciel, Bruno M. Neves, João Martins, Simone Colombo, Maria Teresa Cruz, Pedro Domingues, M. Rosário M. Domingues
Published in: Cellular Signalling, Issue 61, 2019, Page(s) 30-38, ISSN 0898-6568
DOI: 10.1016/j.cellsig.2019.04.015

Exploring the biochemistry of the prenylome and its role in disease through proteomics: progress and potential

Author(s): Maura Brioschi, Alma Martinez Fernandez, Cristina Banfi
Published in: Expert Review of Proteomics, Issue 14/6, 2017, Page(s) 515-528, ISSN 1478-9450
DOI: 10.1080/14789450.2017.1332998

Modification of cysteine residues by cyclopentenone prostaglandins in the elucidation of redox regulation of protein function

Author(s): Dolores Pérez Sala
Published in: Toxicology Letters, Issue 258, 2016, Page(s) S44, ISSN 0378-4274
DOI: 10.1016/j.toxlet.2016.06.1259

Oxidative modifications cross-talk in redox regulation of cellular physiology

Author(s): Eva Griesser, Giulia Coliva, Andreia Mónico, Dolores Pérez-Sala, Maria Fedorova
Published in: Free Radical Biology and Medicine, Issue 108, 2017, Page(s) S47, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2017.04.171

Characterization of vimentin-zinc interaction and its impact on the response to electrophilic and oxidative stress

Author(s): Andreia Mónico, Silvia Zorrilla, Dolores Pérez-Sala
Published in: Free Radical Biology and Medicine, Issue 108, 2017, Page(s) S46, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2017.04.170

Interaction of nitrated/nitroxidized phospholipids with vimentin

Author(s): Tania Melo, Sofia Duarte, Pedro Domingues, Rosario M Domingues, Dolores Pérez-Sala
Published in: Free Radical Biology and Medicine, Issue 108, 2017, Page(s) S52, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2017.04.187

A mass spectrometry approach for the identification and localization of acrolein modifications of proteins

Author(s): Bebiana C. Sousa, Corinne M. Spickett, Andrew R. Pitt
Published in: Free Radical Biology and Medicine, Issue 108, 2017, Page(s) S54, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2017.04.192

Identification and localization of protein-pentanal adducts, a potential lipoxidation marker

Author(s): Catarina Afonso, Andrew R. Pitt, Corinne M. Spickett
Published in: Free Radical Biology and Medicine, Issue 108, 2017, Page(s) S54, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2017.04.193

Alteration of phospholipidome profile in the heart of an animal model of acute myocardial infarction

Author(s): Javier Fernando Montero Bullón, Tania Melo, Tania Martins-Marques, Henrique Girão, Rosário Domingues, Pedro Domingues
Published in: Free Radical Biology and Medicine, Issue 108, 2017, Page(s) S61, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2017.04.214

Aminophospholipid oxidation and glycation in immunity: good or bad?

Author(s): Rosário Domingues, Tania Melo, Elisabete Maciel, Simone Colombo, Pedro Domingues
Published in: Free Radical Biology and Medicine, Issue 108, 2017, Page(s) S61, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2017.04.211

Oxidative metabolism of phosphatidylethanolamines predicted by electrochemistry-mass spectrometry

Author(s): Simone Colombo, Giulia Coliva, Agnieszka Kraj, Jean-Pierre Chervet, Maria Fedorova, Pedro Domingues, M. Rosario Domingues
Published in: Free Radical Biology and Medicine, Issue 108, 2017, Page(s) S62, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2017.04.215

Effects of oxidants and electrophiles on vimentin assembly

Author(s): Andreia Mónico, Sofia Duarte, Maria A. Pajares, Dolores Pérez-Sala
Published in: Free Radical Biology and Medicine, Issue 120, 2018, Page(s) S69, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2018.04.227

The conserved cysteine residue of type III intermediate filaments serves as a structural element and redox sensor

Author(s): Sofia Duarte, Álvaro Viedma-Poyatos, Andreia Mónico, Dolores Pérez-Sala
Published in: Free Radical Biology and Medicine, Issue 120, 2018, Page(s) S84, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2018.04.277

Radical induced oxidation of di-oleoyl phosphatidylserine using mass spectrometry revealed preferential oxydation of Sn-2 oleoyl chain

Author(s): M. Rosário Domingues, Ana Campos, Simone Colombo, Tânia Melo, Pedro Domingues
Published in: Free Radical Biology and Medicine, Issue 120, 2018, Page(s) S118, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2018.04.390

Study of covalent adduction of nitrated phospholipids to peptides using tandem mass spectrometry in different instruments

Author(s): Javier-Fernando Montero-Bullón, Tânia Melo, M Rosário M Domingues, Pedro Domingues
Published in: Free Radical Biology and Medicine, Issue 120, 2018, Page(s) S121, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2018.04.399

European contribution to the study of ROS: A summary of the findings and prospects for the future from the COST action BM1203 (EU-ROS)

Author(s): Javier Egea, Isabel Fabregat, Yves M. Frapart, Pietro Ghezzi, Agnes Görlach, Thomas Kietzmann, Kateryna Kubaichuk, Ulla G. Knaus, Manuela G. Lopez, Gloria Olaso-Gonzalez, Andreas Petry, Rainer Schulz, Jose Vina, Paul Winyard, Kahina Abbas, Opeyemi S. Ademowo, Catarina B. Afonso, Ioanna Andreadou, Haike Antelmann, Fernando Antunes, Mutay Aslan, Markus M. Bachschmid, Rui M. Barbosa, Vsevolod Belous
Published in: Redox Biology, Issue 13, 2017, Page(s) 94-162, ISSN 2213-2317
DOI: 10.1016/j.redox.2017.05.007

Redox lipidomics and adductomics - Advanced analytical strategies to study oxidized lipids and lipid-protein adducts

Author(s): Gabriele Cruciani, Pedro Domingues, Maria Fedorova, Francesco Galli, Corinne M. Spickett
Published in: Free Radical Biology and Medicine, Issue 144, 2019, Page(s) 1-5, ISSN 0891-5849
DOI: 10.1016/j.freeradbiomed.2019.07.027

Single Cell Analysis by High-Resolution Atmospheric-Pressure MALDI MS Imaging

Author(s): Dhaka Ram Bhandari, Giulia Coliva, Maria Fedorova, Bernhard Spengler
Published in: Single Cell Metabolism - Methods and Protocols, Issue 2064, 2020, Page(s) 103-111
DOI: 10.1007/978-1-4939-9831-9_8