Leveraging Pharmaceutical Sciences and Structural Biology Training to develop 21st Century Vaccines

Deliverables

Identification of Rabies antigen for alternative vaccination routes

Identification of RSV antigen for alternative vaccination routes

Immunological properties of the modified GBS antigen

Bio-distribution of lipid nanoparticles

Identification of self-amplifying Rabies RNA construct

Identification of self-amplifying RSV RNA construct

Optimised adjuvant system for GBS antigen presentation

Optimised adjuvant system for a Rabies vaccine

Optimised adjuvant system for an RSV vaccine

Model to investigate intracellular ROS, antigen presentation and specific T cell responses

Evaluating polymeric particles, cationic emulsions and lipoplex formulations

Bio-distribution of adjuvants and associated antigens–mucosal routes

Metabolic factors that contribute to the impaired adaptive immunity in older adults

Key physico-chemical attributes of alum, emulsion, liposome and nanoparticle systems shown to be effective as adjuvants

Bio-distribution of adjuvants and their associated antigen - 1

Comparing the immune response efficacy of antigen and adjuvant combinations - mucosal routes

Optimised adjuvant attributes for self-amplifying RNA vaccines

Bio-distribution of selected polymeric particles, cationic emulsions and lipoplexes

Evaluation of the immune response efficacy

Comparison of cellular uptake in monocyte-derived macrophages and dendritic cells

Structural flexibility of the MenB Antigen

Structural flexibility of the GBS Antigen

Impact of adjuvant design

Identification of T-cell epitopes

Modulation of T-cell epitope affinity

Key biophysical and physico-chemical attributes of current lipid nanoparticles

Bio-distribution of adjuvants and their associated antigen - 2

Adjuvant properties identified

Correlation of adjuvant properties (identified in WP2) with efficacy of immunisation

Interactions of GBS and Rabies antigen and adjuvants

Interactions of GBS and RSV antigen and adjuvants

4 ESRs with the skills to influence, cause an effect, and lead on policy development and economic prosperity in their research field

4 ESRs with the skills and methods to ensure they can enthusiastically convey their science and reach all audiences

Deliver Outreach events at the British Science Festival and using on-line media

4 ESRs trained to PhD level

ESRs who will make a quantifiable difference with their research through engagement with a full range of stakeholders and external partners

Data management plan

Agreement and implementation of a data management plan.

Publications

Delivery of self-amplifying mRNA vaccines by cationic lipid nanoparticles: The impact of cationic lipid selection

Author(s): Gustavo Lou, Giulia Anderluzzi, Signe Tandrup Schmidt, Stuart Woods, Simona Gallorini, Michela Brazzoli, Fabiola Giusti, Ilaria Ferlenghi, Russell Johnson, Craig W. Roberts, Derek T. O'Hagan, Barbara C. Baudner, Yvonne Perrie
Published in: Journal of Controlled Release, Online ahead of print., 2020, Page(s) Online ahead of print., ISSN 0168-3659
Publisher: Elsevier BV
DOI: 10.1016/j.jconrel.2020.06.027

Scalable Manufacturing Processes for Solid Lipid Nanoparticles

Author(s): Giulia Anderluzzi, Gustavo Lou, Yang Su, Yvonne Perrie
Published in: Pharmaceutical Nanotechnology, 7/6, 2019, Page(s) 444-459, ISSN 2211-7385
Publisher: Bentham Science
DOI: 10.2174/2211738507666190925112942

Microfluidic Manufacture of Solid Lipid Nanoparticles: A Case Study on Tristearin-Based Systems.

Author(s): Giulia Anderluzzi, Yvonne Perrie
Published in: Drug Delivery Letters, 09, 2019, ISSN 2210-3031
Publisher: Bentham Science
DOI: 10.2174/2210303109666190807104437

A novel microfluidic-based approach to formulate size-tuneable large unilamellar cationic liposomes: Formulation, cellular uptake and biodistribution investigations

Author(s): Gustavo Lou, Giulia Anderluzzi, Stuart Woods, Craig W. Roberts, Yvonne Perrie
Published in: European Journal of Pharmaceutics and Biopharmaceutics, 143, 2019, Page(s) 51-60, ISSN 0939-6411
Publisher: Elsevier BV
DOI: 10.1016/j.ejpb.2019.08.013

Investigating the Impact of Delivery System Design on the Efficacy of Self-Amplifying RNA Vaccines

Author(s): Giulia Anderluzzi, Gustavo Lou, Simona Gallorini, Michela Brazzoli, Russell Johnson, Derek T. O’Hagan, Barbara C. Baudner, Yvonne Perrie
Published in: Vaccines, 8/2, 2020, Page(s) 212, ISSN 2076-393X
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines8020212

Using microfluidics for scalable manufacturing of nanomedicines from bench to GMP: A case study using protein-loaded liposomes

Author(s): Cameron Webb, Neil Forbes, Carla B. Roces, Giulia Anderluzzi, Gustavo Lou, Suraj Abraham, Logan Ingalls, Keara Marshall, Timothy J. Leaver, Julie A. Watts, Jonathan W. Aylott, Yvonne Perrie
Published in: International Journal of Pharmaceutics, 582, 2020, Page(s) 119266, ISSN 0378-5173
Publisher: Elsevier BV
DOI: 10.1016/j.ijpharm.2020.119266

Mannosylation of LNP Results in Improved Potency for Self-Amplifying RNA (SAM) Vaccines

Author(s): Roshan Goswami, Despo Chatzikleanthous, Gustavo Lou, Fabiola Giusti, Alessandra Bonci, Marianna Taccone, Michela Brazzoli, Simona Gallorini, Ilaria Ferlenghi, Francesco Berti, Derek T. O’Hagan, Carlo Pergola, Barbara C. Baudner, Roberto Adamo
Published in: ACS Infectious Diseases, 5/9, 2019, Page(s) 1546-1558, ISSN 2373-8227
Publisher: ACS
DOI: 10.1021/acsinfecdis.9b00084

Synthesis of protein conjugates adsorbed on cationic liposomes surface

Author(s): Despo Chatzikleanthous, Robert Cunliffe, Filippo Carboni, Maria Rosaria Romano, Derek T. O'Hagan, Craig W. Roberts, Yvonne Perrie, Roberto Adamo
Published in: MethodsX, 7, 2020, Page(s) 100942, ISSN 2215-0161
Publisher: ELSEVIER
DOI: 10.1016/j.mex.2020.100942

Design of a novel vaccine nanotechnology-based delivery system comprising CpGODN-protein conjugate anchored to liposomes

Author(s): Despo Chatzikleanthous, Signe Tandrup Schmidt, Giada Buffi, Ida Paciello, Robert Cunliffe, Filippo Carboni, Maria Rosaria Romano, Derek T. O'Hagan, Ugo D'Oro, Stuart Woods, Craig W. Roberts, Yvonne Perrie, Roberto Adamo
Published in: Journal of Controlled Release, 323, 2020, Page(s) 125-137, ISSN 0168-3659
Publisher: Elsevier BV
DOI: 10.1016/j.jconrel.2020.04.001

Deliverables

Publications

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