Leveraging Pharmaceutical Sciences and Structural Biology Training to develop 21st Century Vaccines

Informazioni relative al progetto

PHA-ST-TRAIN-VAC

ID dell’accordo di sovvenzione: 675370

DOI 10.3030/675370

Progetto chiuso

Data della firma CE 30 Giugno 2016

Data di avvio 1 Giugno 2016

Data di completamento 31 Maggio 2020

Finanziato da

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Costo totale € 1 062 698,40

Contributo UE € 1 062 698,40

Coordinato da

UNIVERSITY OF STRATHCLYDE
United Kingdom

Risultati finali

Documents, reports (26)

Identification of Rabies antigen for alternative vaccination routes

Identification of RSV antigen for alternative vaccination routes

Immunological properties of the modified GBS antigen
Bio-distribution of lipid nanoparticles
Identification of self-amplifying Rabies RNA construct

Identification of self-amplifying RSV RNA construct

Optimised adjuvant system for GBS antigen presentation
Optimised adjuvant system for a Rabies vaccine

Optimised adjuvant system for an RSV vaccine

Model to investigate intracellular ROS, antigen presentation and specific T cell responses
Evaluating polymeric particles, cationic emulsions and lipoplex formulations
Bio-distribution of adjuvants and associated antigens–mucosal routes
Metabolic factors that contribute to the impaired adaptive immunity in older adults
Key physico-chemical attributes of alum, emulsion, liposome and nanoparticle systems shown to be effective as adjuvants
Bio-distribution of adjuvants and their associated antigen - 1
Comparing the immune response efficacy of antigen and adjuvant combinations - mucosal routes
Optimised adjuvant attributes for self-amplifying RNA vaccines
Bio-distribution of selected polymeric particles, cationic emulsions and lipoplexes
Evaluation of the immune response efficacy
Comparison of cellular uptake in monocyte-derived macrophages and dendritic cells
Structural flexibility of the MenB Antigen

Structural flexibility of the GBS Antigen

Impact of adjuvant design
Identification of T-cell epitopes
Modulation of T-cell epitope affinity
Key biophysical and physico-chemical attributes of current lipid nanoparticles
Bio-distribution of adjuvants and their associated antigen - 2
Adjuvant properties identified
Correlation of adjuvant properties (identified in WP2) with efficacy of immunisation
Interactions of GBS and Rabies antigen and adjuvants

Interactions of GBS and RSV antigen and adjuvants

Other (5)

4 ESRs with the skills to influence, cause an effect, and lead on policy development and economic prosperity in their research field
4 ESRs with the skills and methods to ensure they can enthusiastically convey their science and reach all audiences
Deliver Outreach events at the British Science Festival and using on-line media
4 ESRs trained to PhD level
ESRs who will make a quantifiable difference with their research through engagement with a full range of stakeholders and external partners

Open Research Data Pilot (1)

Data management plan

Agreement and implementation of a data management plan.

Pubblicazioni

Peer reviewed articles (9)

Delivery of self-amplifying mRNA vaccines by cationic lipid nanoparticles: The impact of cationic lipid selection

Autori: Gustavo Lou, Giulia Anderluzzi, Signe Tandrup Schmidt, Stuart Woods, Simona Gallorini, Michela Brazzoli, Fabiola Giusti, Ilaria Ferlenghi, Russell Johnson, Craig W. Roberts, Derek T. O'Hagan, Barbara C. Baudner, Yvonne Perrie
Pubblicato in: Journal of Controlled Release, Numero Online ahead of print., 2020, Pagina/e Online ahead of print., ISSN 0168-3659
Editore: Elsevier BV
DOI: 10.1016/j.jconrel.2020.06.027

Scalable Manufacturing Processes for Solid Lipid Nanoparticles

Autori: Giulia Anderluzzi, Gustavo Lou, Yang Su, Yvonne Perrie
Pubblicato in: Pharmaceutical Nanotechnology, Numero 7/6, 2019, Pagina/e 444-459, ISSN 2211-7385
Editore: Bentham Science
DOI: 10.2174/2211738507666190925112942

Microfluidic Manufacture of Solid Lipid Nanoparticles: A Case Study on Tristearin-Based Systems.

Autori: Giulia Anderluzzi, Yvonne Perrie
Pubblicato in: Drug Delivery Letters, Numero 09, 2019, ISSN 2210-3031
Editore: Bentham Science
DOI: 10.2174/2210303109666190807104437

A novel microfluidic-based approach to formulate size-tuneable large unilamellar cationic liposomes: Formulation, cellular uptake and biodistribution investigations

Autori: Gustavo Lou, Giulia Anderluzzi, Stuart Woods, Craig W. Roberts, Yvonne Perrie
Pubblicato in: European Journal of Pharmaceutics and Biopharmaceutics, Numero 143, 2019, Pagina/e 51-60, ISSN 0939-6411
Editore: Elsevier BV
DOI: 10.1016/j.ejpb.2019.08.013

Investigating the Impact of Delivery System Design on the Efficacy of Self-Amplifying RNA Vaccines

Autori: Giulia Anderluzzi, Gustavo Lou, Simona Gallorini, Michela Brazzoli, Russell Johnson, Derek T. O’Hagan, Barbara C. Baudner, Yvonne Perrie
Pubblicato in: Vaccines, Numero 8/2, 2020, Pagina/e 212, ISSN 2076-393X
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/vaccines8020212

Using microfluidics for scalable manufacturing of nanomedicines from bench to GMP: A case study using protein-loaded liposomes

Autori: Cameron Webb, Neil Forbes, Carla B. Roces, Giulia Anderluzzi, Gustavo Lou, Suraj Abraham, Logan Ingalls, Keara Marshall, Timothy J. Leaver, Julie A. Watts, Jonathan W. Aylott, Yvonne Perrie
Pubblicato in: International Journal of Pharmaceutics, Numero 582, 2020, Pagina/e 119266, ISSN 0378-5173
Editore: Elsevier BV
DOI: 10.1016/j.ijpharm.2020.119266

Mannosylation of LNP Results in Improved Potency for Self-Amplifying RNA (SAM) Vaccines

Autori: Roshan Goswami, Despo Chatzikleanthous, Gustavo Lou, Fabiola Giusti, Alessandra Bonci, Marianna Taccone, Michela Brazzoli, Simona Gallorini, Ilaria Ferlenghi, Francesco Berti, Derek T. O’Hagan, Carlo Pergola, Barbara C. Baudner, Roberto Adamo
Pubblicato in: ACS Infectious Diseases, Numero 5/9, 2019, Pagina/e 1546-1558, ISSN 2373-8227
Editore: ACS
DOI: 10.1021/acsinfecdis.9b00084

Synthesis of protein conjugates adsorbed on cationic liposomes surface

Autori: Despo Chatzikleanthous, Robert Cunliffe, Filippo Carboni, Maria Rosaria Romano, Derek T. O'Hagan, Craig W. Roberts, Yvonne Perrie, Roberto Adamo
Pubblicato in: MethodsX, Numero 7, 2020, Pagina/e 100942, ISSN 2215-0161
Editore: ELSEVIER
DOI: 10.1016/j.mex.2020.100942

Design of a novel vaccine nanotechnology-based delivery system comprising CpGODN-protein conjugate anchored to liposomes

Autori: Despo Chatzikleanthous, Signe Tandrup Schmidt, Giada Buffi, Ida Paciello, Robert Cunliffe, Filippo Carboni, Maria Rosaria Romano, Derek T. O'Hagan, Ugo D'Oro, Stuart Woods, Craig W. Roberts, Yvonne Perrie, Roberto Adamo
Pubblicato in: Journal of Controlled Release, Numero 323, 2020, Pagina/e 125-137, ISSN 0168-3659
Editore: Elsevier BV
DOI: 10.1016/j.jconrel.2020.04.001