Illuminating aptamers and ribozymes for biomolecular tagging and fluorogen activation

Project Information

illumizymes

Grant agreement ID: 682586

DOI 10.3030/682586

Project closed

EC signature date 5 April 2016

Start date 1 July 2016

End date 30 June 2022

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 2 061 250,00

EU contribution € 2 061 250,00

Coordinated by

JULIUS-MAXIMILIANS-UNIVERSITAT WURZBURG
Germany

Publications

Peer reviewed articles (18)

Structure and mechanism of the methyltransferase ribozyme MTR1

Author(s): Carolin P. M. Scheitl, Mateusz Mieczkowski, Hermann Schindelin, Claudia Höbartner
Published in: Nature Chemical Biology, Issue monthly, 2022, Page(s) 547-555, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-022-00976-x

Large Stokes shift fluorescence activation in an RNA aptamer by intermolecular proton transfer to guanine

Author(s): Mateusz Mieczkowski, Christian Steinmetzger, Irene Bessi, Ann-Kathrin Lenz, Alexander Schmiedel, Marco Holzapfel, Christoph Lambert, Vladimir Pena, Claudia Höbartner
Published in: Nature Communications, Issue 12:3549, 2021, Page(s) 11, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-23932-0

Mechanism of molnupiravir-induced SARS-CoV-2 mutagenesis

Author(s): Florian Kabinger, Carina Stiller, Jana Schmitzová, Christian Dienemann, Goran Kokic, Hauke S. Hillen, Claudia Höbartner, Patrick Cramer
Published in: Nature Structural & Molecular Biology, Issue 28:740, 2021, Page(s) 7, ISSN 1545-9993
Publisher: Nature Publishing Group
DOI: 10.1038/s41594-021-00651-0

Mechanism of SARS-CoV-2 polymerase stalling by remdesivir

Author(s): Goran Kokic, Hauke S. Hillen, Dimitry Tegunov, Christian Dienemann, Florian Seitz, Jana Schmitzova, Lucas Farnung, Aaron Siewert, Claudia Höbartner, Patrick Cramer
Published in: Nature Communications, Issue 12/1, 2021, Page(s) 7, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-20542-0

The RNA methyltransferase METTL8 installs m3C32 in mitochondrial tRNAsThr/Ser(UCN) to optimise tRNA structure and mitochondrial translation

Author(s): Nicole Kleiber, Nicolas Lemus-Diaz, Carina Stiller, Marleen Heinrichs, Mandy Mong-Quyen Mai, Philipp Hackert, Ricarda Richter-Dennerlein, Claudia Höbartner, Katherine E. Bohnsack, Markus T. Bohnsack
Published in: Nature Communications, Issue 13:209, 2022, Page(s) 19, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-27905-1

RNA-Cleaving Deoxyribozymes Differentiate Methylated Cytidine Isomers in RNA

Author(s): Anam Liaqat, Maksim V. Sednev, Carina Stiller, Claudia Höbartner
Published in: Angewandte Chemie International Edition, Issue 60:19058, 2021, Page(s) 5, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202106517

Fundamental studies of functional nucleic acids: aptamers, riboswitches, ribozymes and DNAzymes

Author(s): Ronald Micura and Claudia Höbartner
Published in: Chemical Society Reviews, Issue 24/year, 2020, ISSN 0306-0012
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0cs00617c

N 6 -Methyladenosine-Sensitive RNA-Cleaving Deoxyribozymes

Author(s): Maksim V. Sednev, Volodymyr Mykhailiuk, Priyanka Choudhury, Julia Halang, Katherine E. Sloan, Markus T. Bohnsack, Claudia Höbartner
Published in: Angewandte Chemie International Edition, Issue 57/46, 2018, Page(s) 15117-15121, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201808745

Debranchase-resistant labeling of RNA using the 10DM24 deoxyribozyme and fluorescent modified nucleotides

Author(s): Tucker J. Carrocci, Lea Lohe, Matthew J. Ashton, Claudia Höbartner, Aaron A. Hoskins
Published in: Chemical Communications, Issue 53/88, 2017, Page(s) 11992-11995, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/c7cc06703h

A Multicolor Large Stokes Shift Fluorogen-Activating RNA Aptamer with Cationic Chromophores

Author(s): Christian Steinmetzger, Navaneethan Palanisamy, Kiran R. Gore, Claudia Höbartner
Published in: Chemistry - A European Journal, Issue weekly, 2018, Page(s) 5, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.201805882

Structure–fluorescence activation relationships of a large Stokes shift fluorogenic RNA aptamer

Author(s): Christian Steinmetzger, Irene Bessi, Ann-Kathrin Lenz, Claudia Höbartner
Published in: Nucleic Acids Research, 2019, Page(s) 2019, 47, 11538-11550, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkz1084

Direct in Vitro Selection of Trans -Acting Ribozymes for Posttranscriptional, Site-Specific, and Covalent Fluorescent Labeling of RNA

Author(s): Mohammad Ghaem Maghami, Carolin P. M. Scheitl, Claudia Höbartner
Published in: Journal of the American Chemical Society, Issue 141/50, 2019, Page(s) 19546-19549, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.9b10531

Supramolecular Fluorescence Resonance Energy Transfer in Nucleobase‐Modified Fluorogenic RNA Aptamers

Author(s): Christian Steinmetzger, Carmen Bäuerlein, Claudia Höbartner
Published in: Angewandte Chemie International Edition, Issue 59/17, 2020, Page(s) 6760-6764, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201916707

Repurposing Antiviral Drugs for Orthogonal RNA‐Catalyzed Labeling of RNA

Author(s): Mohammad Ghaem Maghami, Surjendu Dey, Ann‐Kathrin Lenz, Claudia Höbartner
Published in: Angewandte Chemie International Edition, Issue 59/24, 2020, Page(s) 9335-9339, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202001300

N 6‐isopentenyladenosine in RNA determines the cleavage site of endonuclease deoxyribozymes

Author(s): Anam Liaqat, Carina Stiller, Manuela Michel, Maksim V Sednev, Claudia Höbartner
Published in: Angewandte Chemie International Edition, 2020, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202006218

New Deoxyribozymes for the Native Ligation of RNA

Author(s): Carolin P. M. Scheitl, Sandra Lange, Claudia Höbartner
Published in: Molecules, Issue 25/16, 2020, Page(s) 3650, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules25163650

Site-specific RNA methylation by a methyltransferase ribozyme

Author(s): Carolin P. M. Scheitl, Mohammad Ghaem Maghami, Ann-Kathrin Lenz, Claudia Höbartner
Published in: Nature, Issue 587/7835, 2020, Page(s) 663-667, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-020-2854-z

Tuning Exciton Coupling of Merocyanine Nucleoside Dimers by RNA, DNA and GNA Double Helix Conformations

Author(s): Julia Dietzsch, David Bialas, Johannes Bandorf, Frank Würthner, Claudia Höbartner
Published in: Angewandte Chemie Internation Edition, Issue e202116783, 2022, Page(s) 8, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202116783

Book chapters (1)

In Vitro Selection of Deoxyribozymes for the Detection of RNA Modifications

Author(s): Anam Liaqat, Maksim V. Sednev, Claudia Höbartner
Published in: Ribosome Biogenesis: Methods and Protocols, Methods in Molecular Biology, vol. 2533, 2022, Page(s) 167-179, ISBN 978-1-0716-2501-9
Publisher: Humana Press
DOI: 10.1007/978-1-0716-2501-9_10