Resultado final
Upon assembly of a functional DNA pore, which is verified to insert itself in biological membrane environments, the pore will be analysed using electron cryo-tomography. Subsequently, 4D-models will be created.
4D-model of archaellumUpon expression, purification and biochemical analysis of several wildtype and mutated archaellum subunits, the proteins will be analysed using electron cryo-microscopy. We will use the archaellum proteins from different archaea such as S. acidocaldarius, P.furiosus, M. jannaschii and Archaeoglobus fulgidus. We will also image the intact archaellar motor in situ in Haloferax volcanii cells. It is expected this variety of model organisms will ensure that structural information can be obtained. Subsequently, 4D-models will be created: a description of the mechanism that accounts for function.
Based on existing DNA origami design software a novel bioinformatic toolkit will be created to enable the automated protein embedding function into the DNA scaffold using the 4D models from WP3
A website that will facilitate internal and external communication and dissemination.
A Data Management Plan will be elaborated within the first six months of the project, guaranteeing an appropriate disclosure of research data to the public and standardised data management within the project. The types of data that will be generated and collected within the project comprise design data (oligonucleotide sequences, technical drawings, etc.), laboratory test data (images, reaction parameters, etc.), software and metadata (final results/scientific insights, documentation, reports, publications, etc.). Three different levels of confidentiality will be defined according to IP issues and marketing strategies: classified data, IPR relevant data and public data.
Publicaciones
Autores:
Lena Hoffmann, Katrin Anders, Lisa F. Bischof, Xing Ye, Julia Reimann, Sunia Khadouma, Trong K. Pham, Chris van der Does, Phillip C. Wright, Lars-Oliver Essen, Sonja-Verena Albers
Publicado en:
Journal of Biological Chemistry, Edición 294/18, 2019, Página(s) 7460-7471, ISSN 0021-9258
Editor:
American Society for Biochemistry and Molecular Biology Inc.
DOI:
10.1074/jbc.ra119.007709
Autores:
Tadija Kekic, Ivan Barisic
Publicado en:
Computational and Structural Biotechnology Journal, Edición 18, 2020, Página(s) 1191-1201, ISSN 2001-0370
Editor:
Elsevier
DOI:
10.1016/j.csbj.2020.05.016
Autores:
Yasaman Ahmadi, Ashley L. Nord, Amanda J. Wilson, Christiane Hütter, Fabian Schroeder, Morgan Beeby, Ivan Barišić
Publicado en:
Small, Edición 16/22, 2020, Página(s) 2001855, ISSN 1613-6810
Editor:
Wiley - V C H Verlag GmbbH & Co.
DOI:
10.1002/smll.202001855
Autores:
Zhengqun Li, Yoshiaki Kinosita, Marta Rodriguez-Franco, Phillip Nußbaum, Frank Braun, Floriane Delpech, Tessa E. F. Quax, Sonja-Verena Albers
Publicado en:
mBio, Edición 10/3, 2019, Página(s) e00377-19, ISSN 2150-7511
Editor:
American Society for Microbiology
DOI:
10.1128/mBio.00377-19
Autores:
Morgan Beeby, Josie L Ferreira, Patrick Tripp, Sonja-Verena Albers, David R Mitchell
Publicado en:
FEMS Microbiology Reviews, Edición 44/3, 2020, Página(s) 253-304, ISSN 0168-6445
Editor:
Blackwell
DOI:
10.1093/femsre/fuaa006
Autores:
Yasaman Ahmadi, Ivan Barisic
Publicado en:
Journal of Visualized Experiments, Edición 143, 2019, ISSN 1940-087X
Editor:
MYJoVE Corporation
DOI:
10.3791/58771
Autores:
Lena Hoffmann, Andreas Schummer, Julia Reimann, Maria F. Haurat, Amanda J. Wilson, Morgan Beeby, Bettina Warscheid, Sonja-V. Albers
Publicado en:
MicrobiologyOpen, Edición 6/1, 2017, Página(s) e00414, ISSN 2045-8827
Editor:
John Wiley and Sons Inc.
DOI:
10.1002/mbo3.414
Autores:
Yasaman Ahmadi, Elisa De Llano, Ivan Barišić
Publicado en:
Nanoscale, Edición 10/16, 2018, Página(s) 7494-7504, ISSN 2040-3364
Editor:
Royal Society of Chemistry
DOI:
10.1039/C7NR09461B
Autores:
M. Florencia Haurat, Ana Sofia Figueiredo, Lena Hoffmann, Lingling Li, Katharina Herr, Amanda J. Wilson, Morgan Beeby, Jörg Schaber, Sonja-Verena Albers
Publicado en:
Molecular Microbiology, Edición 103/1, 2017, Página(s) 181-194, ISSN 0950-382X
Editor:
Blackwell Publishing Inc.
DOI:
10.1111/mmi.13550
Autores:
Haichao Miao, Elisa De Llano, Johannes Sorger, Yasaman Ahmadi, Tadija Kekic, Tobias Isenberg, M. Eduard Groller, Ivan Barisic, Ivan Viola
Publicado en:
IEEE Transactions on Visualization and Computer Graphics, Edición 24/1, 2018, Página(s) 1014-1024, ISSN 1077-2626
Editor:
Institute of Electrical and Electronics Engineers
DOI:
10.1109/TVCG.2017.2743981
Autores:
H. Miao, E. De Llano, T. Isenberg, M. E. Gröller, I. Barišić, I. Viola
Publicado en:
Computer Graphics Forum, Edición 37/3, 2018, Página(s) 403-413, ISSN 0167-7055
Editor:
Blackwell Publishing Inc.
DOI:
10.1111/cgf.13429
Autores:
Elisa de Llano, Haichao Miao, Yasaman Ahmadi, Amanda J Wilson, Morgan Beeby, Ivan Viola, Ivan Barisic
Publicado en:
Nucleic Acids Research, Edición 48/15, 2020, Página(s) 8269-8275, ISSN 0305-1048
Editor:
Oxford University Press
DOI:
10.1093/nar/gkaa593
Autores:
Chi-Lin Tsai, Patrick Tripp, Shamphavi Sivabalasarma, Changyi Zhang, Marta Rodriguez-Franco, Rebecca L. Wipfler, Paushali Chaudhury, Ankan Banerjee, Morgan Beeby, Rachel J. Whitaker, John A. Tainer, Sonja-Verena Albers
Publicado en:
Nature Microbiology, Edición 5/1, 2020, Página(s) 216-225, ISSN 2058-5276
Editor:
Nature
DOI:
10.1038/s41564-019-0622-3
Autores:
Dominik Esser, Lena Hoffmann, Trong Khoa Pham, Christopher Bräsen, Wen Qiu, Phillip C. Wright, Sonja-Verena Albers, Bettina Siebers
Publicado en:
FEMS Microbiology Reviews, Edición 40/5, 2016, Página(s) 625-647, ISSN 1574-6976
Editor:
OXFORD UNIVERSITY PRESS
DOI:
10.1093/femsre/fuw020
Autores:
Maximilian Sbardellati, Haichao Miao, Hsiang-Yun Wu, Eduard Gröller, Ivan Barisic, Ivan Viola
Publicado en:
VCBM 19: Eurographics Workshop on Visual Computing for Biology and Medicine, 2019, ISBN 978-3-03868-081-9
Editor:
The Eurographics Association
DOI:
10.2312/vcbm.20191237
Derechos de propiedad intelectual
Número de solicitud/publicación:
EP
16801263
Fecha:
2016-11-25
Solicitante(s):
AIT AUSTRIAN INSTITUTE OF TECHNOLOGY GMBH
Número de solicitud/publicación:
EP
16806004
Fecha:
2016-11-25
Solicitante(s):
AIT AUSTRIAN INSTITUTE OF TECHNOLOGY GMBH
Número de solicitud/publicación:
EP
16801262
Fecha:
2016-11-25
Solicitante(s):
AIT AUSTRIAN INSTITUTE OF TECHNOLOGY GMBH
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