Molecular Analytical Robotics Assays

Livrables

Upon assembly of a functional DNA pore, which is verified to insert itself in biological membrane environments, the pore will be analysed using electron cryo-tomography. Subsequently, 4D-models will be created.

4D-model of archaellum

Upon expression, purification and biochemical analysis of several wildtype and mutated archaellum subunits, the proteins will be analysed using electron cryo-microscopy. We will use the archaellum proteins from different archaea such as S. acidocaldarius, P.furiosus, M. jannaschii and Archaeoglobus fulgidus. We will also image the intact archaellar motor in situ in Haloferax volcanii cells. It is expected this variety of model organisms will ensure that structural information can be obtained. Subsequently, 4D-models will be created: a description of the mechanism that accounts for function.

DNA origami design software

Based on existing DNA origami design software a novel bioinformatic toolkit will be created to enable the automated protein embedding function into the DNA scaffold using the 4D models from WP3

Collaborative website

A website that will facilitate internal and external communication and dissemination.

Data management plan

A Data Management Plan will be elaborated within the first six months of the project, guaranteeing an appropriate disclosure of research data to the public and standardised data management within the project. The types of data that will be generated and collected within the project comprise design data (oligonucleotide sequences, technical drawings, etc.), laboratory test data (images, reaction parameters, etc.), software and metadata (final results/scientific insights, documentation, reports, publications, etc.). Three different levels of confidentiality will be defined according to IP issues and marketing strategies: classified data, IPR relevant data and public data.

Publications

Structure and interactions of the archaeal motility repression module ArnA–ArnB that modulates archaellum gene expression in Sulfolobus acidocaldarius

Auteurs: Lena Hoffmann, Katrin Anders, Lisa F. Bischof, Xing Ye, Julia Reimann, Sunia Khadouma, Trong K. Pham, Chris van der Does, Phillip C. Wright, Lars-Oliver Essen, Sonja-Verena Albers
Publié dans: Journal of Biological Chemistry, Numéro 294/18, 2019, Page(s) 7460-7471, ISSN 0021-9258
Éditeur: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra119.007709

In silico modelling of DNA nanostructures

Auteurs: Tadija Kekic, Ivan Barisic
Publié dans: Computational and Structural Biotechnology Journal, Numéro 18, 2020, Page(s) 1191-1201, ISSN 2001-0370
Éditeur: Elsevier
DOI: 10.1016/j.csbj.2020.05.016

The Brownian and Flow‐Driven Rotational Dynamics of a Multicomponent DNA Origami‐Based Rotor

Auteurs: Yasaman Ahmadi, Ashley L. Nord, Amanda J. Wilson, Christiane Hütter, Fabian Schroeder, Morgan Beeby, Ivan Barišić
Publié dans: Small, Numéro 16/22, 2020, Page(s) 2001855, ISSN 1613-6810
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.202001855

Positioning of the Motility Machinery in Halophilic Archaea

Auteurs: Zhengqun Li, Yoshiaki Kinosita, Marta Rodriguez-Franco, Phillip Nußbaum, Frank Braun, Floriane Delpech, Tessa E. F. Quax, Sonja-Verena Albers
Publié dans: mBio, Numéro 10/3, 2019, Page(s) e00377-19, ISSN 2150-7511
Éditeur: American Society for Microbiology
DOI: 10.1128/mBio.00377-19

Propulsive nanomachines: the convergent evolution of archaella, flagella and cilia

Auteurs: Morgan Beeby, Josie L Ferreira, Patrick Tripp, Sonja-Verena Albers, David R Mitchell
Publié dans: FEMS Microbiology Reviews, Numéro 44/3, 2020, Page(s) 253-304, ISSN 0168-6445
Éditeur: Blackwell
DOI: 10.1093/femsre/fuaa006

Gene-therapy Inspired Polycation Coating for Protection of DNA Origami Nanostructures

Auteurs: Yasaman Ahmadi, Ivan Barisic
Publié dans: Journal of Visualized Experiments, Numéro 143, 2019, ISSN 1940-087X
Éditeur: MYJoVE Corporation
DOI: 10.3791/58771

Expanding the archaellum regulatory network - the eukaryotic protein kinases ArnC and ArnD influence motility of Sulfolobus acidocaldarius

Auteurs: Lena Hoffmann, Andreas Schummer, Julia Reimann, Maria F. Haurat, Amanda J. Wilson, Morgan Beeby, Bettina Warscheid, Sonja-V. Albers
Publié dans: MicrobiologyOpen, Numéro 6/1, 2017, Page(s) e00414, ISSN 2045-8827
Éditeur: John Wiley and Sons Inc.
DOI: 10.1002/mbo3.414

(Poly)cation-induced protection of conventional and wireframe DNA origami nanostructures

Auteurs: Yasaman Ahmadi, Elisa De Llano, Ivan Barišić
Publié dans: Nanoscale, Numéro 10/16, 2018, Page(s) 7494-7504, ISSN 2040-3364
Éditeur: Royal Society of Chemistry
DOI: 10.1039/C7NR09461B

ArnS, a kinase involved in starvation-induced archaellum expression

Auteurs: M. Florencia Haurat, Ana Sofia Figueiredo, Lena Hoffmann, Lingling Li, Katharina Herr, Amanda J. Wilson, Morgan Beeby, Jörg Schaber, Sonja-Verena Albers
Publié dans: Molecular Microbiology, Numéro 103/1, 2017, Page(s) 181-194, ISSN 0950-382X
Éditeur: Blackwell Publishing Inc.
DOI: 10.1111/mmi.13550

Multiscale Visualization and Scale-Adaptive Modification of DNA Nanostructures

Auteurs: Haichao Miao, Elisa De Llano, Johannes Sorger, Yasaman Ahmadi, Tadija Kekic, Tobias Isenberg, M. Eduard Groller, Ivan Barisic, Ivan Viola
Publié dans: IEEE Transactions on Visualization and Computer Graphics, Numéro 24/1, 2018, Page(s) 1014-1024, ISSN 1077-2626
Éditeur: Institute of Electrical and Electronics Engineers
DOI: 10.1109/TVCG.2017.2743981

DimSUM: Dimension and Scale Unifying Map for Visual Abstraction of DNA Origami Structures

Auteurs: H. Miao, E. De Llano, T. Isenberg, M. E. Gröller, I. Barišić, I. Viola
Publié dans: Computer Graphics Forum, Numéro 37/3, 2018, Page(s) 403-413, ISSN 0167-7055
Éditeur: Blackwell Publishing Inc.
DOI: 10.1111/cgf.13429

Adenita: interactive 3D modelling and visualization of DNA nanostructures

Auteurs: Elisa de Llano, Haichao Miao, Yasaman Ahmadi, Amanda J Wilson, Morgan Beeby, Ivan Viola, Ivan Barisic
Publié dans: Nucleic Acids Research, Numéro 48/15, 2020, Page(s) 8269-8275, ISSN 0305-1048
Éditeur: Oxford University Press
DOI: 10.1093/nar/gkaa593

The structure of the periplasmic FlaG–FlaF complex and its essential role for archaellar swimming motility

Auteurs: Chi-Lin Tsai, Patrick Tripp, Shamphavi Sivabalasarma, Changyi Zhang, Marta Rodriguez-Franco, Rebecca L. Wipfler, Paushali Chaudhury, Ankan Banerjee, Morgan Beeby, Rachel J. Whitaker, John A. Tainer, Sonja-Verena Albers
Publié dans: Nature Microbiology, Numéro 5/1, 2020, Page(s) 216-225, ISSN 2058-5276
Éditeur: Nature
DOI: 10.1038/s41564-019-0622-3

Protein phosphorylation and its role in archaeal signal transduction

Auteurs: Dominik Esser, Lena Hoffmann, Trong Khoa Pham, Christopher Bräsen, Wen Qiu, Phillip C. Wright, Sonja-Verena Albers, Bettina Siebers
Publié dans: FEMS Microbiology Reviews, Numéro 40/5, 2016, Page(s) 625-647, ISSN 1574-6976
Éditeur: OXFORD UNIVERSITY PRESS
DOI: 10.1093/femsre/fuw020

Interactive Exploded Views for Molecular Structures

Auteurs: Maximilian Sbardellati, Haichao Miao, Hsiang-Yun Wu, Eduard Gröller, Ivan Barisic, Ivan Viola
Publié dans: VCBM 19: Eurographics Workshop on Visual Computing for Biology and Medicine, 2019, ISBN 978-3-03868-081-9
Éditeur: The Eurographics Association
DOI: 10.2312/vcbm.20191237

Droits de propriété intellectuelle

AUTONOMOUS SENSING MOLECULES (ASM)

Numéro de demande/publication: EP 16801263
Date: 2016-11-25

MOLECULAR ROBOT

Numéro de demande/publication: EP 16806004
Date: 2016-11-25

NANOSTRUCTURES WITH CATALYTIC ACTIVITY

Numéro de demande/publication: EP 16801262
Date: 2016-11-25

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