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PROtein SEQuencing using Optical single molecule real-time detection

Objective

The advent of analytical techniques with extremely low limits of detection has led to dramatic progresses mostly in the field of nucleic acids sequencing. Despite the advent of the next generation sequencing platforms, the current genome sequencing task remains formidable, and revolutionary advances in DNA sequencing technology are still demanded. Nevertheless, the primary actors in virtually all life processes are the proteins coded by DNA sequences known as genes. Proteins can yield far more compelling revelations than may be gleaned from DNA alone. Protein sequencing may radically transform patient treatment, enabling precise monitoring of disease response to therapeutics at the molecular level. Single-molecule sequencing of proteins is of enormous value, offering the potential to detect diminishingly small quantities of proteins that may have been altered by alternative splicing or post-translational modification. In this project, we build upon current state-of-the-art sequencing technologies to develop novel proof-of-principle technologies for high-throughput protein sequencing and single molecule DNA/RNA sequencing. The work proposed herein will provide: (i) a new sequencing technology development that utilizes plasmonic nanostructures in order to enhance the optical detection and to control the molecules movement by means of optical trapping; (ii) a novel approach of plasmonic based optical spectroscopy for sequencing of protein; (iii), a rigorous analytical model to reconstruct the exact sequence from the signals recorded; and (iv) a plasmonic device that can perform both nucleic acids and amino-acids sequencing in one functional unit. These research efforts provide a foundation for the novel use of systems for a wide range of applications, such as the framework to investigate next generation protein sequencing, as well as high-throughput DNA sequencing and genetic diagnostics.

Field of science

  • /natural sciences/biological sciences/biochemistry/biomolecules/nucleic acid
  • /natural sciences/chemical sciences/analytical chemistry/spectroscopy
  • /natural sciences/biological sciences/biochemistry/biomolecules/proteins
  • /natural sciences/biological sciences/genetics and heredity/genome

Call for proposal

H2020-FETOPEN-2014-2015-RIA
See other projects for this call

Funding Scheme

RIA - Research and Innovation action

Coordinator

FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA
Address
Via Morego 30
16163 Genova
Italy
Activity type
Research Organisations
EU contribution
€ 811 663,75

Participants (4)

UNIVERSITE PARIS-SACLAY
France
EU contribution
€ 709 708,75
Address
Immeuble Technologique Entree B Rte De L Orme Aux Merisiers
91190 Saint Aubin
Activity type
Higher or Secondary Education Establishments
UNIVERSITAT DE BARCELONA
Spain
EU contribution
€ 649 643,75
Address
Gran Via De Les Corts Catalanes 585
08007 Barcelona
Activity type
Higher or Secondary Education Establishments
ALACRIS THERANOSTICS GMBH
Germany
EU contribution
€ 374 625
Address
Max Planck Strasse 3
12489 Berlin
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
AB ANALITICA SRL
Italy
EU contribution
€ 361 160
Address
Via Svizzera 16
35127 Padova
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)