Objective
The advent of analytical techniques with extremely low limits of detection has led to dramatic progresses mostly in the field of nucleic acids sequencing. Despite the advent of the next generation sequencing platforms, the current genome sequencing task remains formidable, and revolutionary advances in DNA sequencing technology are still demanded. Nevertheless, the primary actors in virtually all life processes are the proteins coded by DNA sequences known as genes. Proteins can yield far more compelling revelations than may be gleaned from DNA alone. Protein sequencing may radically transform patient treatment, enabling precise monitoring of disease response to therapeutics at the molecular level. Single-molecule sequencing of proteins is of enormous value, offering the potential to detect diminishingly small quantities of proteins that may have been altered by alternative splicing or post-translational modification. In this project, we build upon current state-of-the-art sequencing technologies to develop novel proof-of-principle technologies for high-throughput protein sequencing and single molecule DNA/RNA sequencing. The work proposed herein will provide: (i) a new sequencing technology development that utilizes plasmonic nanostructures in order to enhance the optical detection and to control the molecules movement by means of optical trapping; (ii) a novel approach of plasmonic based optical spectroscopy for sequencing of protein; (iii), a rigorous analytical model to reconstruct the exact sequence from the signals recorded; and (iv) a plasmonic device that can perform both nucleic acids and amino-acids sequencing in one functional unit. These research efforts provide a foundation for the novel use of systems for a wide range of applications, such as the framework to investigate next generation protein sequencing, as well as high-throughput DNA sequencing and genetic diagnostics.
Fields of science
- natural sciencescomputer and information sciencesdata science
- natural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomics
- natural sciencesbiological sciencesbiochemistrybiomoleculesnucleic acids
- natural sciencesbiological sciencesgeneticsDNA
- natural scienceschemical sciencesorganic chemistryamines
Programme(s)
Funding Scheme
RIA - Research and Innovation action
Coordinator
16163 Genova
Italy
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Participants (5)
91190 Gif-sur-yvette
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Legal entity other than a subcontractor which is affiliated or legally linked to a participant. The entity carries out work under the conditions laid down in the Grant Agreement, supplies goods or provides services for the action, but did not sign the Grant Agreement. A third party abides by the rules applicable to its related participant under the Grant Agreement with regard to eligibility of costs and control of expenditure.
75794 Paris
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08007 Barcelona
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12489 Berlin
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The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.
35127 Padova
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The organization defined itself as SME (small and medium-sized enterprise) at the time the Grant Agreement was signed.