PROtein SEQuencing using Optical single molecule real-time detection

Deliverables

Demonstrators, pilots, prototypes (8)

Demonstrator of SERS detection of single amino-acid

Demonstrator of SERS detection of single amino-acid

Demonstrator of integrated platform with micro/nanofluidics and nanotrapping elements

Demonstrator of integrated platform with micro/nanofluidics and nanotrapping elements.

Demonstrator of FRET intensity and lifetime spectroscopic detection

Demonstrator of FRET intensity and lifetime spectroscopic detection

Demonstrator of multiple plasmonic nanotrap platform

Demonstrator of multiple plasmonic nanotrap platform

Computational demonstration of final protocol of detection

Computational demonstration of final protocol of detection

Demonstrator of steerable optical trap

Demonstrator of steerable optical trap

Demonstrator of integrated optical system

Lead: IIT/ Deputy:UPSUD Demonstrator of integrated optical system.

Demonstration of SMS

Demonstration of SMS

Documents, reports (9)

Online publication of the PROSEQO website

Online publication of the PROSEQO website

Public report on sequencing tests n.1

Lead participant: AB ANALITICA /Deputy: ALACRIS Reports on sequencing tests

Public report on sequencing tests n.3

Lead participant: AB ANALITICA /Deputy: ALACRIS Reports on sequencing tests

Report on project communication N.2

Lead participant: IIT /Deputy: AB ANALITICA Report on project communication

Report on nanofabrication process for multi-nanochannel integrated system

Report on nanofabrication process for multi-nanochannel integrated system

Report on project communication n.1

Report on project communication Lead participant: IIT /Deputy: AB ANALITICA Reports on sequencing tests

Report on design of multi-nanochannel nanostructure integrated with plasmonic nanotrapping

Report on design of multi-nanochannel nanostructure integrated with plasmonic nanotrapping.

Public version of the Final Report

Public version of the Final Report

Public report on sequencing tests n.2

Lead participant: AB ANALITICA /Deputy: ALACRIS Public reports on sequencing tests

Other (2)

Data management plan

Data management plan

Data management plan-end period

Data management plan

Publications

Peer reviewed articles (14)

Plasmonic zero mode waveguide for highly confined and enhanced fluorescence emission

Author(s): Paolo Ponzellini, Xavier Zambrana-Puyalto, Nicolò Maccaferri, Luca Lanzanò, Francesco De Angelis, Denis Garoli
Published in: Nanoscale, 10/36, 2018, Page(s) 17362-17369, ISSN 2040-3364
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8nr04103b

Hybrid plasmonic nanostructures based on controlled integration of MoS 2 flakes on metallic nanoholes

Author(s): Denis Garoli, Dario Mosconi, Ermanno Miele, Nicolò Maccaferri, Matteo Ardini, Giorgia Giovannini, Michele Dipalo, Stefano Agnoli, Francesco De Angelis
Published in: Nanoscale, 10/36, 2018, Page(s) 17105-17111, ISSN 2040-3364
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8nr05026k

Autofluorescence-Free Live-Cell Imaging Using Terbium Nanoparticles

Author(s): M. Cardoso Dos Santos, J. Goetz, H. Bartenlian, K.-L. Wong, L. J. Charbonnière, N. Hildebrandt
Published in: Bioconjugate Chemistry, 29/4, 2018, Page(s) 1327-1334, ISSN 1043-1802
Publisher: American Chemical Society
DOI: 10.1021/acs.bioconjchem.8b00069

Nanosurface Energy Transfer from Long-Lifetime Terbium Donors to Gold Nanoparticles

Author(s): Chi Chen, Clyde Midelet, Shashi Bhuckory, Niko Hildebrandt, Martinus H. V. Werts
Published in: The Journal of Physical Chemistry C, 122/30, 2018, Page(s) 17566-17574, ISSN 1932-7447
Publisher: American Chemical Society
DOI: 10.1021/acs.jpcc.8b06539

Quantum Dot-Based FRET Immunoassay for HER2 Using Ultrasmall Affinity Proteins

Author(s): Yu-Tang Wu, Xue Qiu, Sarah Lindbo, Kimihiro Susumu, Igor L. Medintz, Sophia Hober, Niko Hildebrandt
Published in: Small, 14/35, 2018, Page(s) 1802266, ISSN 1613-6810
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.201802266

Simple, Amplified, and Multiplexed Detection of MicroRNAs Using Time-Gated FRET and Hybridization Chain Reaction

Author(s): Jiajia Guo, Carlos Mingoes, Xue Qiu, Niko Hildebrandt
Published in: Analytical Chemistry, 91/4, 2019, Page(s) 3101-3109, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.8b05600

Three-Dimensional FRET Multiplexing for DNA Quantification with Attomolar Detection Limits

Author(s): Xue Qiu, Jiajia Guo, Jingyue Xu, Niko Hildebrandt
Published in: The Journal of Physical Chemistry Letters, 9/15, 2018, Page(s) 4379-4384, ISSN 1948-7185
Publisher: American Chemical Society
DOI: 10.1021/acs.jpclett.8b01944

Advanced microRNA-based cancer diagnostics using amplified time-gated FRET

Author(s): Xue Qiu, Jingyue Xu, Jiajia Guo, Akram Yahia-Ammar, Nikiforos-Ioannis Kapetanakis, Isabelle Duroux-Richard, Julia J. Unterluggauer, Nicole Golob-Schwarzl, Christophe Regeard, Catherine Uzan, Sébastien Gouy, Michael DuBow, Johannes Haybaeck, Florence Apparailly, Pierre Busson, Niko Hildebrandt
Published in: Chemical Science, 9/42, 2018, Page(s) 8046-8055, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/c8sc03121e

Optimizing FRET on Aluminum Surfaces via Controlled Attachment of Fluorescent Dyes

Author(s): Alexandra Petreto, Marcelina Cardoso Dos Santos, Olivier Lefebvre, Gabriel Ribeiro Dos Santos, Paolo Ponzellini, Denis Garoli, Francesco De Angelis, Mehdi Ammar, Niko Hildebrandt
Published in: ACS Omega, 3/12, 2018, Page(s) 18867-18876, ISSN 2470-1343
Publisher: American Chemical Society
DOI: 10.1021/acsomega.8b02774

Single-Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots

Author(s): Chi Chen, Lijiao Ao, Yu-Tang Wu, Vjona Cifliku, Marcelina Cardoso Dos Santos, Emmanuel Bourrier, Martina Delbianco, David Parker, Jurriaan M. Zwier, Liang Huang, Niko Hildebrandt
Published in: Angewandte Chemie International Edition, 57/41, 2018, Page(s) 13686-13690, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201807585

Conformational Details of Quantum Dot-DNA Resolved by Förster Resonance Energy Transfer Lifetime Nanoruler

Author(s): Jiajia Guo, Xue Qiu, Carlos Mingoes, Jeffrey R. Deschamps, Kimihiro Susumu, Igor L. Medintz, Niko Hildebrandt
Published in: ACS Nano, 13/1, 2018, Page(s) 505-514, ISSN 1936-0851
Publisher: American Chemical Society
DOI: 10.1021/acsnano.8b07137

Force feedback effects on single molecule hopping and pulling experiments

Author(s): M. Rico-Pasto, I. Pastor, F. Ritort
Published in: The Journal of Chemical Physics, 148/12, 2018, Page(s) 123327, ISSN 0021-9606
Publisher: American Institute of Physics
DOI: 10.1063/1.5010303

Hot electrons in water: injection and ponderomotive acceleration by means of plasmonic nanoelectrodes

Author(s): Pierfrancesco Zilio, Michele Dipalo, Francesco Tantussi, Gabriele C Messina, Francesco de Angelis
Published in: Light: Science & Applications, 6/6, 2017, Page(s) e17002, ISSN 2047-7538
Publisher: Nature publisher
DOI: 10.1038/lsa.2017.2

Beaming of Helical Light from Plasmonic Vortices via Adiabatically Tapered Nanotip

Author(s): Denis Garoli, Pierfrancesco Zilio, Yuri Gorodetski, Francesco Tantussi, Francesco De Angelis
Published in: Nano Letters, 16/10, 2016, Page(s) 6636-6643, ISSN 1530-6984
Publisher: American Chemical Society
DOI: 10.1021/acs.nanolett.6b03359