PROtein SEQuencing using Optical single molecule real-time detection

Livrables

Demonstrator of SERS detection of single amino-acid
Demonstrator of integrated platform with micro/nanofluidics and nanotrapping elements

Demonstrator of integrated platform with micro/nanofluidics and nanotrapping elements.

Demonstrator of FRET intensity and lifetime spectroscopic detection
Demonstrator of multiple plasmonic nanotrap platform
Computational demonstration of final protocol of detection
Demonstrator of steerable optical trap
Demonstrator of integrated optical system

Lead: IIT/ Deputy:UPSUD Demonstrator of integrated optical system.

Demonstration of SMS

Online publication of the PROSEQO website
Public report on sequencing tests n.1

Lead participant: AB ANALITICA /Deputy: ALACRIS Reports on sequencing tests

Public report on sequencing tests n.3

Lead participant: AB ANALITICA /Deputy: ALACRIS Reports on sequencing tests

Report on project communication N.2

Lead participant: IIT /Deputy: AB ANALITICA Report on project communication

Report on nanofabrication process for multi-nanochannel integrated system
Report on project communication n.1

Report on project communication Lead participant: IIT /Deputy: AB ANALITICA Reports on sequencing tests

Report on design of multi-nanochannel nanostructure integrated with plasmonic nanotrapping

Report on design of multi-nanochannel nanostructure integrated with plasmonic nanotrapping.

Public version of the Final Report
Public report on sequencing tests n.2

Lead participant: AB ANALITICA /Deputy: ALACRIS Public reports on sequencing tests

Data management plan
Data management plan-end period

Data management plan

Publications

Plasmonic zero mode waveguide for highly confined and enhanced fluorescence emission

Auteurs: Paolo Ponzellini, Xavier Zambrana-Puyalto, Nicolò Maccaferri, Luca Lanzanò, Francesco De Angelis, Denis Garoli
Publié dans: Nanoscale, Numéro 10/36, 2018, Page(s) 17362-17369, ISSN 2040-3364
Éditeur: Royal Society of Chemistry
DOI: 10.1039/c8nr04103b

Hybrid plasmonic nanostructures based on controlled integration of MoS 2 flakes on metallic nanoholes

Auteurs: Denis Garoli, Dario Mosconi, Ermanno Miele, Nicolò Maccaferri, Matteo Ardini, Giorgia Giovannini, Michele Dipalo, Stefano Agnoli, Francesco De Angelis
Publié dans: Nanoscale, Numéro 10/36, 2018, Page(s) 17105-17111, ISSN 2040-3364
Éditeur: Royal Society of Chemistry
DOI: 10.1039/c8nr05026k

Autofluorescence-Free Live-Cell Imaging Using Terbium Nanoparticles

Auteurs: M. Cardoso Dos Santos, J. Goetz, H. Bartenlian, K.-L. Wong, L. J. Charbonnière, N. Hildebrandt
Publié dans: Bioconjugate Chemistry, Numéro 29/4, 2018, Page(s) 1327-1334, ISSN 1043-1802
Éditeur: American Chemical Society
DOI: 10.1021/acs.bioconjchem.8b00069

Nanosurface Energy Transfer from Long-Lifetime Terbium Donors to Gold Nanoparticles

Auteurs: Chi Chen, Clyde Midelet, Shashi Bhuckory, Niko Hildebrandt, Martinus H. V. Werts
Publié dans: The Journal of Physical Chemistry C, Numéro 122/30, 2018, Page(s) 17566-17574, ISSN 1932-7447
Éditeur: American Chemical Society
DOI: 10.1021/acs.jpcc.8b06539

Quantum Dot-Based FRET Immunoassay for HER2 Using Ultrasmall Affinity Proteins

Auteurs: Yu-Tang Wu, Xue Qiu, Sarah Lindbo, Kimihiro Susumu, Igor L. Medintz, Sophia Hober, Niko Hildebrandt
Publié dans: Small, Numéro 14/35, 2018, Page(s) 1802266, ISSN 1613-6810
Éditeur: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.201802266

Simple, Amplified, and Multiplexed Detection of MicroRNAs Using Time-Gated FRET and Hybridization Chain Reaction

Auteurs: Jiajia Guo, Carlos Mingoes, Xue Qiu, Niko Hildebrandt
Publié dans: Analytical Chemistry, Numéro 91/4, 2019, Page(s) 3101-3109, ISSN 0003-2700
Éditeur: American Chemical Society
DOI: 10.1021/acs.analchem.8b05600

Three-Dimensional FRET Multiplexing for DNA Quantification with Attomolar Detection Limits

Auteurs: Xue Qiu, Jiajia Guo, Jingyue Xu, Niko Hildebrandt
Publié dans: The Journal of Physical Chemistry Letters, Numéro 9/15, 2018, Page(s) 4379-4384, ISSN 1948-7185
Éditeur: American Chemical Society
DOI: 10.1021/acs.jpclett.8b01944

Advanced microRNA-based cancer diagnostics using amplified time-gated FRET

Auteurs: Xue Qiu, Jingyue Xu, Jiajia Guo, Akram Yahia-Ammar, Nikiforos-Ioannis Kapetanakis, Isabelle Duroux-Richard, Julia J. Unterluggauer, Nicole Golob-Schwarzl, Christophe Regeard, Catherine Uzan, Sébastien Gouy, Michael DuBow, Johannes Haybaeck, Florence Apparailly, Pierre Busson, Niko Hildebrandt
Publié dans: Chemical Science, Numéro 9/42, 2018, Page(s) 8046-8055, ISSN 2041-6520
Éditeur: Royal Society of Chemistry
DOI: 10.1039/c8sc03121e

Optimizing FRET on Aluminum Surfaces via Controlled Attachment of Fluorescent Dyes

Auteurs: Alexandra Petreto, Marcelina Cardoso Dos Santos, Olivier Lefebvre, Gabriel Ribeiro Dos Santos, Paolo Ponzellini, Denis Garoli, Francesco De Angelis, Mehdi Ammar, Niko Hildebrandt
Publié dans: ACS Omega, Numéro 3/12, 2018, Page(s) 18867-18876, ISSN 2470-1343
Éditeur: American Chemical Society
DOI: 10.1021/acsomega.8b02774

Single-Nanoparticle Cell Barcoding by Tunable FRET from Lanthanides to Quantum Dots

Auteurs: Chi Chen, Lijiao Ao, Yu-Tang Wu, Vjona Cifliku, Marcelina Cardoso Dos Santos, Emmanuel Bourrier, Martina Delbianco, David Parker, Jurriaan M. Zwier, Liang Huang, Niko Hildebrandt
Publié dans: Angewandte Chemie International Edition, Numéro 57/41, 2018, Page(s) 13686-13690, ISSN 1433-7851
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/anie.201807585

Conformational Details of Quantum Dot-DNA Resolved by Förster Resonance Energy Transfer Lifetime Nanoruler

Auteurs: Jiajia Guo, Xue Qiu, Carlos Mingoes, Jeffrey R. Deschamps, Kimihiro Susumu, Igor L. Medintz, Niko Hildebrandt
Publié dans: ACS Nano, Numéro 13/1, 2018, Page(s) 505-514, ISSN 1936-0851
Éditeur: American Chemical Society
DOI: 10.1021/acsnano.8b07137

Force feedback effects on single molecule hopping and pulling experiments

Auteurs: M. Rico-Pasto, I. Pastor, F. Ritort
Publié dans: The Journal of Chemical Physics, Numéro 148/12, 2018, Page(s) 123327, ISSN 0021-9606
Éditeur: American Institute of Physics
DOI: 10.1063/1.5010303

Hot electrons in water: injection and ponderomotive acceleration by means of plasmonic nanoelectrodes

Auteurs: Pierfrancesco Zilio, Michele Dipalo, Francesco Tantussi, Gabriele C Messina, Francesco de Angelis
Publié dans: Light: Science & Applications, Numéro 6/6, 2017, Page(s) e17002, ISSN 2047-7538
Éditeur: Nature publisher
DOI: 10.1038/lsa.2017.2

Beaming of Helical Light from Plasmonic Vortices via Adiabatically Tapered Nanotip

Auteurs: Denis Garoli, Pierfrancesco Zilio, Yuri Gorodetski, Francesco Tantussi, Francesco De Angelis
Publié dans: Nano Letters, Numéro 16/10, 2016, Page(s) 6636-6643, ISSN 1530-6984
Éditeur: American Chemical Society
DOI: 10.1021/acs.nanolett.6b03359

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