A portable MicroNanoBioSystem and Instrument for ultra-fast analysis of pathogens in food: Innovation from LOVE-FOOD lab prototype to a pre-commercial instrument

Informations projet

LoveFood2Market

N° de convention de subvention: 687681

DOI

10.3030/687681

Projet clôturé

Date de signature de la CE 10 Novembre 2015

Date de début 1 Février 2016

Date de fin 30 Avril 2019

Financé au titre de

• INDUSTRIAL LEADERSHIP - Leadership in enabling and industrial technologies - Information and Communication Technologies (ICT)

Coût total

€ 3 474 732,50

Contribution de l’UE

€ 3 151 750,00

Coordonné par

IDRYMA TECHNOLOGIAS KAI EREVNAS
Greece

CORDIS fournit des liens vers les livrables publics et les publications des projets HORIZON.

Les liens vers les livrables et les publications des projets du 7e PC, ainsi que les liens vers certains types de résultats spécifiques tels que les jeux de données et les logiciels, sont récupérés dynamiquement sur OpenAIRE .

Livrables

Documents, reports (16)

Integrated chips validated for detection of all targeted bacteria

Report with validation tests results

Optimized PCR and RPA protocols for the efficient DNA amplification of 4 different pathogens

Report with protocol description

Environmental risk assessment associated with magnetic micro/nanoparticles applied for bioassays.

report on environmental risk assessment associated with magnetic micro/nanoparticles applied for bioassays.

Optimized protocol for capturing beads carrying bacteria cells and cell lysis

report with protocol description

ChipA fabricated and validated for detection of Salmonella.

Report on chipA fabrication and validation tests results

Optimized protocol for short pre-culturing, isolation and concentration of bacteria from food using beads

report with protocol description

Optimized acoustic detection protocols for 4 pathogens using the PCR or the RPA amplification method

Report with protocols descriptions

Optimized method for acoustic signal enhancement after amplification using liposomes

Report with amplification method description

Report on the shelf life of beads and the functionalized chips

Report (see title)

Immunosorbents with desired specificity and binding capacity for bacteria cell capturing

Report on immunosorbents

Platforms encompassing 4 integrated chips fabricated and operated

Report on platform fabrication and tests results

Bead trapping on chip. Report on design, fabrication, evaluation and chip prototype

Report on design, fabrication, evaluation and chip prototype

Protocol and software for statistical analysis of bacteria sampling requirements

Report on protocol software description for statistical analysis of bacteria sampling

Report on SAW chip recycling capability
ChipB fabricated and validated for detection of Salmonella. Report and chipB prototype.

Report on fabrication of ChipB and validation tests results

Optimized protocol for DNA purification

Report with protocol description

Websites, patent fillings, videos etc. (3)

Project web page established

web page

At least 3 high impact publications and / or review papers

3 publications

Organization of workshops in international conferences on Food-Safety control

Report on workshops participation in international conferences on Food-Safety control

Publications

Peer reviewed articles (12)

A modular integrated lab-on-a-chip platform for fast and highly efficient sample preparation for foodborne pathogen screening

Auteurs: K. Tsougeni, A.S. Kastania, G.D. Kaprou, Michael Eck, Gerhard Jobst, P.S. Petrou, S.E. Kakabakos, D. Mastellos, E. Gogolides, A. Tserepi
Publié dans: Sensors and Actuators B: Chemical, Numéro 288, 2019, Page(s) 171-179, ISSN 0925-4005
Éditeur: Elsevier BV
DOI: 10.1016/j.snb.2019.02.070

3D-printed Point-of-Care Platform for Genetic Testing of Infectious Diseases Directly in Human Samples Using Acoustic Sensors and a Smartphone

Auteurs: George Papadakis, Alexandros K. Pantazis, Maria Ntogka, Konstantinos Parasyris, Gesthimani-Ioanna Theodosi, Georgia Kaprou, Electra Gizeli
Publié dans: ACS Sensors, Numéro 4/5, 2019, Page(s) 1329-1336, ISSN 2379-3694
Éditeur: ACS Publications
DOI: 10.1021/acssensors.9b00264

Micro-nano-bio acoustic system for the detection of foodborne pathogens in real samples

Auteurs: George Papadakis, Pavla Murasova, Audrey Hamiot, Katerina Tsougeni, Georgia Kaprou, Michael Eck, David Rabus, Zuzana Bilkova, Bruno Dupuy, Gerhard Jobst, Angeliki Tserepi, Evangelos Gogolides, Electra Gizeli
Publié dans: Biosensors and Bioelectronics, Numéro 111, 2018, Page(s) 52-58, ISSN 0956-5663
Éditeur: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2018.03.056

Three-dimensional (3D) plasma micro-nanotextured slides for high performance biomolecule microarrays: Comparison with epoxy-silane coated glass slides

Auteurs: Katerina Tsougeni, Kosmas Ellinas, George Koukouvinos, Panagiota S. Petrou, Angeliki Tserepi, Sotirios E. Kakabakos, Evangelos Gogolides
Publié dans: Colloids and Surfaces B: Biointerfaces, Numéro 165, 2018, Page(s) 270-277, ISSN 0927-7765
Éditeur: Elsevier BV
DOI: 10.1016/j.colsurfb.2018.02.055

Advanced immunocapture of milk-borne Salmonella by microfluidic magnetically stabilized fluidized bed

Auteurs: Jana Srbova, Pavla Krulisova, Lucie Holubova, Iago Pereiro, Amel Bendali, Audrey Hamiot, Veronika Podzemna, Jan Macak, Bruno Dupuy, Stephanie Descroix, Jean-Louis Viovy, Zuzana Bilkova
Publié dans: ELECTROPHORESIS, Numéro 39/3, 2018, Page(s) 526-533, ISSN 0173-0835
Éditeur: John Wiley & Sons Ltd.
DOI: 10.1002/elps.201700257

Stable hydrophilization of FR4 and polyimide-based substrates implemented in microfluidics-on-PCB

Auteurs: E. Cunaj, P.S. Petrou, G.D. Kaprou, S.E. Kakabakos, E. Gogolides, A. Tserepi
Publié dans: Surface and Coatings Technology, Numéro 334, 2018, Page(s) 292-299, ISSN 0257-8972
Éditeur: Elsevier BV
DOI: 10.1016/j.surfcoat.2017.11.039

Optimized acoustic biochip integrated with microfluidics for biomarkers detection in molecular diagnostics

Auteurs: G. Papadakis, J. M. Friedt, M. Eck, D. Rabus, G. Jobst, E. Gizeli
Publié dans: Biomedical Microdevices, Numéro 19/3, 2017, ISSN 1387-2176
Éditeur: Kluwer Academic Publishers
DOI: 10.1007/s10544-017-0159-2

Sample-to-answer acoustic detection of DNA in complex samples

Auteurs: George Papadakis, Pasquale Palladino, Dimitra Chronaki, Achilleas Tsortos, Electra Gizeli
Publié dans: Chem. Commun., Numéro 53/57, 2017, Page(s) 8058-8061, ISSN 1359-7345
Éditeur: Royal Society of Chemistry
DOI: 10.1039/c6cc10175e

Roughness threshold for cell attachment and proliferation on plasma micro-nanotextured polymeric surfaces: the case of primary human skin fibroblasts and mouse immortalized 3T3 fibroblasts

Auteurs: A Bourkoula, V Constantoudis, D Kontziampasis, P S Petrou, S E Kakabakos, A Tserepi, E Gogolides
Publié dans: Journal of Physics D: Applied Physics, Numéro 49/30, 2016, Page(s) 304002, ISSN 0022-3727
Éditeur: Institute of Physics Publishing
DOI: 10.1088/0022-3727/49/30/304002

The lab-on-PCB approach: tackling the μTAS commercial upscaling bottleneck

Auteurs: Despina Moschou, Angeliki Tserepi
Publié dans: Lab Chip, Numéro 17/8, 2017, Page(s) 1388-1405, ISSN 1473-0197
Éditeur: Royal Society of Chemistry
DOI: 10.1039/c7lc00121e

Binding kinetics of bacteria cells on immobilized antibodies in microfluidic channels: Modeling and experiments

Auteurs: Athina S. Kastania, Katerina Tsougeni, Vassilios Constantoudis, Evangelos Gogolides
Publié dans: Sensors and Actuators B: Chemical, Numéro 253, 2017, Page(s) 247-257, ISSN 0925-4005
Éditeur: Elsevier BV
DOI: 10.1016/j.snb.2017.06.113

Transition between stable hydrophilization and fast etching/hydrophilization of poly(methyl)methacrylate polymer using a novel atmospheric pressure dielectric barrier discharge source

Auteurs: Panagiotis Dimitrakellis, Evangelos Gogolides, Angelos Zeniou, Kamil Awsiuk, Jakub Rysz, Mateusz M. Marzec
Publié dans: Journal of Vacuum Science & Technology A: Vacuum, Surfaces, and Films, Numéro 35/4, 2017, Page(s) 041303, ISSN 0734-2101
Éditeur: American Institute of Physics
DOI: 10.1116/1.4984613

Book chapters (1)

3D Plasma Nanotextured® Polymeric Surfaces for Protein or Antibody Arrays, and Biomolecule and Cell Patterning

Auteurs: Katerina Tsougeni, Kosmas Ellinas, George Koukouvinos, Panagiota S. Petrou, Angeliki Tserepi, Sotirios E. Kakabakos, Evangelos Gogolides
Publié dans: Cell-Based Microarrays - Methods and Protocols, Numéro 1771, 2018, Page(s) 27-40, ISBN 978-1-4939-7791-8
Éditeur: Springer New York
DOI: 10.1007/978-1-4939-7792-5_3