A portable MicroNanoBioSystem and Instrument for ultra-fast analysis of pathogens in food: Innovation from LOVE-FOOD lab prototype to a pre-commercial instrument

Projektinformationen

LoveFood2Market

ID Finanzhilfevereinbarung: 687681

DOI 10.3030/687681

Projekt abgeschlossen

EK-Unterschriftsdatum 10 November 2015

Startdatum 1 Februar 2016

Enddatum 30 April 2019

Finanziert unter

• INDUSTRIAL LEADERSHIP - Leadership in enabling and industrial technologies - Information and Communication Technologies (ICT)

Gesamtkosten € 3 474 732,50

EU-Beitrag € 3 151 750,00

Koordiniert durch

IDRYMA TECHNOLOGIAS KAI EREVNAS
Greece

Leistungen

Documents, reports (16)

Integrated chips validated for detection of all targeted bacteria

Report with validation tests results

Optimized PCR and RPA protocols for the efficient DNA amplification of 4 different pathogens

Report with protocol description

Environmental risk assessment associated with magnetic micro/nanoparticles applied for bioassays.

report on environmental risk assessment associated with magnetic micro/nanoparticles applied for bioassays.

Optimized protocol for capturing beads carrying bacteria cells and cell lysis

report with protocol description

ChipA fabricated and validated for detection of Salmonella.

Report on chipA fabrication and validation tests results

Optimized protocol for short pre-culturing, isolation and concentration of bacteria from food using beads

report with protocol description

Optimized acoustic detection protocols for 4 pathogens using the PCR or the RPA amplification method

Report with protocols descriptions

Optimized method for acoustic signal enhancement after amplification using liposomes

Report with amplification method description

Report on the shelf life of beads and the functionalized chips

Report (see title)

Immunosorbents with desired specificity and binding capacity for bacteria cell capturing

Report on immunosorbents

Platforms encompassing 4 integrated chips fabricated and operated

Report on platform fabrication and tests results

Bead trapping on chip. Report on design, fabrication, evaluation and chip prototype

Report on design, fabrication, evaluation and chip prototype

Protocol and software for statistical analysis of bacteria sampling requirements

Report on protocol software description for statistical analysis of bacteria sampling

Report on SAW chip recycling capability
ChipB fabricated and validated for detection of Salmonella. Report and chipB prototype.

Report on fabrication of ChipB and validation tests results

Optimized protocol for DNA purification

Report with protocol description

Websites, patent fillings, videos etc. (3)

Project web page established

web page

At least 3 high impact publications and / or review papers

3 publications

Organization of workshops in international conferences on Food-Safety control

Report on workshops participation in international conferences on Food-Safety control

Veröffentlichungen

Peer reviewed articles (12)

A modular integrated lab-on-a-chip platform for fast and highly efficient sample preparation for foodborne pathogen screening

Autoren: K. Tsougeni, A.S. Kastania, G.D. Kaprou, Michael Eck, Gerhard Jobst, P.S. Petrou, S.E. Kakabakos, D. Mastellos, E. Gogolides, A. Tserepi
Veröffentlicht in: Sensors and Actuators B: Chemical, Ausgabe 288, 2019, Seite(n) 171-179, ISSN 0925-4005
Herausgeber: Elsevier BV
DOI: 10.1016/j.snb.2019.02.070

3D-printed Point-of-Care Platform for Genetic Testing of Infectious Diseases Directly in Human Samples Using Acoustic Sensors and a Smartphone

Autoren: George Papadakis, Alexandros K. Pantazis, Maria Ntogka, Konstantinos Parasyris, Gesthimani-Ioanna Theodosi, Georgia Kaprou, Electra Gizeli
Veröffentlicht in: ACS Sensors, Ausgabe 4/5, 2019, Seite(n) 1329-1336, ISSN 2379-3694
Herausgeber: ACS Publications
DOI: 10.1021/acssensors.9b00264

Micro-nano-bio acoustic system for the detection of foodborne pathogens in real samples

Autoren: George Papadakis, Pavla Murasova, Audrey Hamiot, Katerina Tsougeni, Georgia Kaprou, Michael Eck, David Rabus, Zuzana Bilkova, Bruno Dupuy, Gerhard Jobst, Angeliki Tserepi, Evangelos Gogolides, Electra Gizeli
Veröffentlicht in: Biosensors and Bioelectronics, Ausgabe 111, 2018, Seite(n) 52-58, ISSN 0956-5663
Herausgeber: Pergamon Press Ltd.
DOI: 10.1016/j.bios.2018.03.056

Three-dimensional (3D) plasma micro-nanotextured slides for high performance biomolecule microarrays: Comparison with epoxy-silane coated glass slides

Autoren: Katerina Tsougeni, Kosmas Ellinas, George Koukouvinos, Panagiota S. Petrou, Angeliki Tserepi, Sotirios E. Kakabakos, Evangelos Gogolides
Veröffentlicht in: Colloids and Surfaces B: Biointerfaces, Ausgabe 165, 2018, Seite(n) 270-277, ISSN 0927-7765
Herausgeber: Elsevier BV
DOI: 10.1016/j.colsurfb.2018.02.055

Advanced immunocapture of milk-borne Salmonella by microfluidic magnetically stabilized fluidized bed

Autoren: Jana Srbova, Pavla Krulisova, Lucie Holubova, Iago Pereiro, Amel Bendali, Audrey Hamiot, Veronika Podzemna, Jan Macak, Bruno Dupuy, Stephanie Descroix, Jean-Louis Viovy, Zuzana Bilkova
Veröffentlicht in: ELECTROPHORESIS, Ausgabe 39/3, 2018, Seite(n) 526-533, ISSN 0173-0835
Herausgeber: John Wiley & Sons Ltd.
DOI: 10.1002/elps.201700257

Stable hydrophilization of FR4 and polyimide-based substrates implemented in microfluidics-on-PCB

Autoren: E. Cunaj, P.S. Petrou, G.D. Kaprou, S.E. Kakabakos, E. Gogolides, A. Tserepi
Veröffentlicht in: Surface and Coatings Technology, Ausgabe 334, 2018, Seite(n) 292-299, ISSN 0257-8972
Herausgeber: Elsevier BV
DOI: 10.1016/j.surfcoat.2017.11.039

Optimized acoustic biochip integrated with microfluidics for biomarkers detection in molecular diagnostics

Autoren: G. Papadakis, J. M. Friedt, M. Eck, D. Rabus, G. Jobst, E. Gizeli
Veröffentlicht in: Biomedical Microdevices, Ausgabe 19/3, 2017, ISSN 1387-2176
Herausgeber: Kluwer Academic Publishers
DOI: 10.1007/s10544-017-0159-2

Sample-to-answer acoustic detection of DNA in complex samples

Autoren: George Papadakis, Pasquale Palladino, Dimitra Chronaki, Achilleas Tsortos, Electra Gizeli
Veröffentlicht in: Chem. Commun., Ausgabe 53/57, 2017, Seite(n) 8058-8061, ISSN 1359-7345
Herausgeber: Royal Society of Chemistry
DOI: 10.1039/c6cc10175e

Roughness threshold for cell attachment and proliferation on plasma micro-nanotextured polymeric surfaces: the case of primary human skin fibroblasts and mouse immortalized 3T3 fibroblasts

Autoren: A Bourkoula, V Constantoudis, D Kontziampasis, P S Petrou, S E Kakabakos, A Tserepi, E Gogolides
Veröffentlicht in: Journal of Physics D: Applied Physics, Ausgabe 49/30, 2016, Seite(n) 304002, ISSN 0022-3727
Herausgeber: Institute of Physics Publishing
DOI: 10.1088/0022-3727/49/30/304002

The lab-on-PCB approach: tackling the μTAS commercial upscaling bottleneck

Autoren: Despina Moschou, Angeliki Tserepi
Veröffentlicht in: Lab Chip, Ausgabe 17/8, 2017, Seite(n) 1388-1405, ISSN 1473-0197
Herausgeber: Royal Society of Chemistry
DOI: 10.1039/c7lc00121e

Binding kinetics of bacteria cells on immobilized antibodies in microfluidic channels: Modeling and experiments

Autoren: Athina S. Kastania, Katerina Tsougeni, Vassilios Constantoudis, Evangelos Gogolides
Veröffentlicht in: Sensors and Actuators B: Chemical, Ausgabe 253, 2017, Seite(n) 247-257, ISSN 0925-4005
Herausgeber: Elsevier BV
DOI: 10.1016/j.snb.2017.06.113

Transition between stable hydrophilization and fast etching/hydrophilization of poly(methyl)methacrylate polymer using a novel atmospheric pressure dielectric barrier discharge source

Autoren: Panagiotis Dimitrakellis, Evangelos Gogolides, Angelos Zeniou, Kamil Awsiuk, Jakub Rysz, Mateusz M. Marzec
Veröffentlicht in: Journal of Vacuum Science & Technology A: Vacuum, Surfaces, and Films, Ausgabe 35/4, 2017, Seite(n) 041303, ISSN 0734-2101
Herausgeber: American Institute of Physics
DOI: 10.1116/1.4984613

Book chapters (1)

3D Plasma Nanotextured® Polymeric Surfaces for Protein or Antibody Arrays, and Biomolecule and Cell Patterning

Autoren: Katerina Tsougeni, Kosmas Ellinas, George Koukouvinos, Panagiota S. Petrou, Angeliki Tserepi, Sotirios E. Kakabakos, Evangelos Gogolides
Veröffentlicht in: Cell-Based Microarrays - Methods and Protocols, Ausgabe 1771, 2018, Seite(n) 27-40, ISBN 978-1-4939-7791-8
Herausgeber: Springer New York
DOI: 10.1007/978-1-4939-7792-5_3