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Sexual Plant Reproduction – Seed formation

Deliverables

Papers and Review preparation

Papers and Review preparation thats is reponsible from all particiapnats

Final Report

Final report of the project

First report

The coordinator in collaboration with the leaders of each WP will produce a report every 12 months that will be available to the consortium.

Compilation of results and dissemination of methodologies to partners and the broader community. 4

Compilation of results and dissemination of methodologies to partners and the broader community.

Compilation of results and dissemination of methodologies to partners and the broader community
Activity of molecular markers for siRNA pathway

Activity of molecular markers for siRNA pathway, using AGO5::YFP;

Dissemination using Twitter and Facebook

Dissemination using Twitter and Facebook From the reponsability of all participants in the consortium

Organization of Workshop 1

One workshop will be organized by P1 (UPORTO) at the beginning of the project, on the interdisciplinary approach to investigate PLANT REPRODUCTION at the very beginning of SEXSEED action. All consortium members will have the possibility to meet and organize the researcher movements from Europe to America/Australia and vice-versa.

Organization of Final Workshop

A final workshop will be organized in Australia. This last workshop will be to evaluate the achievements of SEXSEED.

Seed Quality of each transgenic line obtained
Organization of Workshop 2

Meetings organized by the coordinator will be important for presentation of all data obtained. These meetings will be opened to all the scientific community as interactive International Workshops.

Design and implementation of the SEXSEED webpage

The coordinator will create a project webpage in the first months; this will have a public domain and a restricted protected area exclusively for the members of the consortium. Members of the consortium will be able to share protocols, results, reports and other information needed for the progression of the project. The establishment of this online presence is an excellent way to engage the community, attract scientific collaborators, post-doctoral staff and students for the future and to communicate novel scientific results.

Publications

MADS-Box and bHLH Transcription Factors Coordinate TransmittingTract Development in Arabidopsis thaliana

Author(s): Di Marzo M, Roig-Villanova I, Zanchetti E, Caselli F, Gregis V, Bardetti P, Chiara M, Guazzotti A, Caporali E, Mendes MA, Colombo L, Kater MM
Published in: Frontiers in Plant Science, 2020
Publisher: Frontiers Media SA
DOI: 10.3389/fpls.2020.00526

The RNA-dependent DNA methylation pathway is required to restrict SPOROCYTELESS/NOZZLE expression to specify a single female germ cell precursor in Arabidopsis

Author(s): Marta A. Mendes, Rosanna Petrella, Mara Cucinotta, Edoardo Vignati, Stefano Gatti, Sara C. Pinto, Dayton C. Bird, Veronica Gregis, Hugh Dickinson, Matthew R. Tucker, Lucia Colombo
Published in: Development, Issue 147/23, 2020, Page(s) dev194274, ISSN 0950-1991
Publisher: The Company of Biologists Ltd.
DOI: 10.1242/dev.194274

Alternative Splicing Generates a MONOPTEROS Isoform Required for Ovule Development

Author(s): Mara Cucinotta, Alex Cavalleri, Andrea Guazzotti, Chiara Astori, Silvia Manrique, Aureliano Bombarely, Stefania Oliveto, Stefano Biffo, Dolf Weijers, Martin M. Kater, Lucia Colombo
Published in: Current Biology, 2020, ISSN 0960-9822
Publisher: Cell Press
DOI: 10.1016/j.cub.2020.11.026

Three Decades of Advances in Arabinogalactan-Protein Biosynthesis

Author(s): Jessy Silva, Ricardo Ferraz, Paul Dupree, Allan M. Showalter, Sílvia Coimbra
Published in: Frontiers in Plant Science, Issue 11, 2020, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2020.610377

Exploring the Role of Cell Wall-Related Genes and Polysaccharides during Plant Development

Author(s): Matthew Tucker, Haoyu Lou, Matthew Aubert, Laura Wilkinson, Alan Little, Kelly Houston, Sara Pinto, Neil Shirley
Published in: Plants, Issue 7/2, 2018, Page(s) 42, ISSN 2223-7747
Publisher: Plants
DOI: 10.3390/plants7020042

Arabinogalactan Proteins as Interactors along the Crosstalk between the Pollen Tube and the Female Tissues

Author(s): Ana M. Pereira, Ana L. Lopes, Sílvia Coimbra
Published in: Frontiers in Plant Science, Issue 7, 2016, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2016.01895

Revisiting the Female Germline and Its Expanding Toolbox

Author(s): Sara C. Pinto, Marta A. Mendes, Sílvia Coimbra, Matthew R. Tucker
Published in: Trends in Plant Science, 2019, ISSN 1360-1385
Publisher: Elsevier BV
DOI: 10.1016/j.tplants.2019.02.003

There are secrets along the pollen tube pathway, still in need to be discovered

Author(s): Ana Lúcia Lopes, Diana Moreira, Maria João Ferreira, Ana Marta Pereira, Sílvia Coimbra
Published in: Journal of Experimental Botany, 2019, ISSN 0022-0957
Publisher: Oxford University Press
DOI: 10.1093/jxb/erz087

Effect of wheat infection timing on Fusarium head blight causal agents and secondary metabolites in grain

Author(s): Giovanni Beccari, Consuelo Arellano, Lorenzo Covarelli, Francesco Tini, Michael Sulyok, Christina Cowger
Published in: International Journal of Food Microbiology, Issue 290, 2019, Page(s) 214-225, ISSN 0168-1605
Publisher: Elsevier BV
DOI: 10.1016/j.ijfoodmicro.2018.10.014

Establishing a framework for female germline initiation in the plant ovule

Author(s): Jorge Lora, Xiujuan Yang, Mathew R Tucker
Published in: Journal of Experimental Botany, Issue 70/11, 2019, Page(s) 2937-2949, ISSN 0022-0957
Publisher: Oxford University Press
DOI: 10.1093/jxb/erz212

Genetic insights into the modification of the pre-fertilization mechanisms during plant domestication

Author(s): Silvia Manrique, James Friel, Pietro Gramazio, Tomas Hasing, Ignacio Ezquer, Aureliano Bombarely
Published in: Journal of Experimental Botany, Issue 70/11, 2019, Page(s) 3007-3019, ISSN 0022-0957
Publisher: Oxford University Press
DOI: 10.1093/jxb/erz231

Plant Reproduction: AMOR Enables Males to Respond to Female Signals

Author(s): Thomas Dresselhaus, Silvia Coimbra
Published in: Current Biology, Issue 26/8, 2016, Page(s) R321-R323, ISSN 0960-9822
Publisher: Cell Press
DOI: 10.1016/j.cub.2016.03.019

In silico and expression analyses of fasciclin-like arabinogalactan proteins reveal functional conservation during embryo and seed development

Author(s): Mário Costa, Ana Marta Pereira, Sara Cristina Pinto, Jessy Silva, Luís Gustavo Pereira, Sílvia Coimbra
Published in: Plant Reproduction, Issue 32/4, 2019, Page(s) 353-370, ISSN 2194-7953
Publisher: Springer Verlag
DOI: 10.1007/s00497-019-00376-7

Developmental Analysis of Mimulus Seed Transcriptomes Reveals Functional Gene Expression Clusters and Four Imprinted, Endosperm-Expressed Genes

Author(s): Miguel A. Flores-Vergara, Elen Oneal, Mario Costa, Gonzalo Villarino, Caitlyn Roberts, Maria Angels De Luis Balaguer, Sílvia Coimbra, John Willis, Robert G. Franks
Published in: Frontiers in Plant Science, Issue 11, 2020, ISSN 1664-462X
Publisher: Frontiers Media S. A.
DOI: 10.3389/fpls.2020.00132

The best CRISPR/Cas9 versus RNA interference approaches for Arabinogalactan proteins’ study

Author(s): Diana Moreira, Ana Marta Pereira, Ana Lúcia Lopes, Sílvia Coimbra
Published in: Molecular Biology Reports, 2020, ISSN 0301-4851
Publisher: Kluwer Academic Publishers
DOI: 10.1007/s11033-020-05258-0

Arabinogalactan proteins and pectin distribution during female gametogenesis in Quercus suber L.

Author(s): Ana Lúcia Lopes, Mário Luís Costa, Rómulo Sobral, Maria Manuela Costa, Maria Isabel Amorim, Sílvia Coimbra
Published in: Annals of Botany, Issue 117/6, 2016, Page(s) 949-961, ISSN 0305-7364
Publisher: Oxford University Press
DOI: 10.1093/aob/mcw019

Advances in plant reproduction: from gametes to seeds

Author(s): Ana Marta Pereira, Sílvia Coimbra
Published in: Journal of Experimental Botany, Issue 70/11, 2019, Page(s) 2933-2936, ISSN 0022-0957
Publisher: Oxford University Press
DOI: 10.1093/jxb/erz227

Insights into secrets along the pollen tube pathway in need to be discovered

Author(s): Ana Lúcia Lopes, Diana Moreira, Maria João Ferreira, Ana Marta Pereira, Sílvia Coimbra
Published in: Journal of Experimental Botany, Issue 70/11, 2019, Page(s) 2979-2992, ISSN 0022-0957
Publisher: Oxford University Press
DOI: 10.1093/jxb/erz087

In Situ/Subcellular Localization of Arabinogalactan Protein Expression by Fluorescent In Situ Hybridization (FISH)

Author(s): Mário Luís da Costa, María-Teresa Solís, Pilar S. Testillano, Sílvia Coimbra
Published in: The Plant Cell Wall - Methods and Protocols, Issue 2149, 2020, Page(s) 403-427, ISBN 978-1-0716-0619-3
Publisher: Springer New York
DOI: 10.1007/978-1-0716-0621-6_23

Arabidopsis thaliana Pollen Tube Culture for Multi-omics Studies

Author(s): Mario Costa, Jessy Silva, Silvia Coimbra
Published in: Pollen and Pollen Tube Biology - Methods and Protocols, Issue 2160, 2020, Page(s) 149-165, ISBN 978-1-0716-0671-1
Publisher: Springer US
DOI: 10.1007/978-1-0716-0672-8_10

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