Exploring the Chemical Biology of Sequence Space via Picoliter Droplets

Publications

A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution

Author(s): Che Fai Alex Wong, Liisa van Vliet, Swapnil Vilas Bhujbal, Chengzhi Guo, Marit Sletmoen, Bjørn Torger Stokke, Florian Hollfelder, Rahmi Lale
Published in: ACS Synthetic Biology, 2021, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.1c00084

Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase

Author(s): J. David Schnettler, Oskar James Klein, Tomasz S. Kaminski, Pierre-Yves Colin, Florian Hollfelder
Published in: J Am Chem Soc, 2023, Page(s) 1083–1096, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.2c10673

Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

Author(s): Maximilian Gantz, Stefanie Neun, Elliot J. Medcalf, Liisa D. van Vliet, Florian Hollfelder
Published in: Chemical Reviews, 2023, Page(s) ASAP, ISSN 0009-2665
Publisher: American Chemical Society
DOI: 10.1021/acs.chemrev.2c00910

Microgel culture and spatial identity mapping elucidate the signalling requirements for primate epiblast and amnion formation

Author(s): Clara Munger, Timo N. Kohler, Erin Slatery, Anna L. Ellermann, Sophie Bergmann, Christopher A. Penfold, Ioakeim Ampartzidis, Yutong Chen, Florian Hollfelder, Thorsten E. Boroviak
Published in: Development, 2022, Page(s) dev200263, ISSN 1477-9129
Publisher: The company of biologists
DOI: 10.1242/dev.200263

UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution

Author(s): Paul Jannis Zurek, Philipp Knyphausen, Katharina Neufeld, Ahir Pushpanath, Florian Hollfelder
Published in: Nature Communications, Issue 11/1, 2020, Page(s) 6023, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-19687-9

Enzymatic N-Allylation of Primary and Secondary Amines Using Renewable Cinnamic Acids Enabled by Bacterial Reductive Aminases

Author(s): Godwin A. Aleku, Gabriel R. Titchiner, George W. Roberts, Sasha R. Derrington, James R. Marshall, Florian Hollfelder, Nicholas J. Turner, and David Leys
Published in: ACS Sustainable Chem. Eng., Issue 2168-0485, 2022, Page(s) 6794–6806, ISSN 2168-0485
Publisher: American Chemical Society
DOI: 10.1021/acssuschemeng.2c01180

Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture.

Author(s): Knyphausen P, Rangel Pereira M, Brear P, Hyvönen M, Jermutus L, Hollfelder F.
Published in: Nature Communications, Issue 14, 2023, Page(s) 768, ISSN 1608-3199
Publisher: Nature
DOI: 10.1038/s41467-023-36099-7

Engineering the protein dynamics of an ancestral luciferase

Author(s): Andrea Schenkmayerova, Gaspar P. Pinto, Martin Toul, Martin Marek, Lenka Hernychova, Joan Planas-Iglesias, Veronika Daniel Liskova, Daniel Pluskal, Michal Vasina, Stephane Emond, Mark Dörr, Radka Chaloupkova, David Bednar, Zbynek Prokop, Florian Hollfelder, Uwe T. Bornscheuer, Jiri Damborsky
Published in: Nature Communications, Issue 12/1, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-23450-z

Self-Organization of Mouse Stem Cells into an Extended Potential Blastoid

Author(s): Berna Sozen, Andy L. Cox, Joachim De Jonghe, Min Bao, Florian Hollfelder, David M. Glover, Magdalena Zernicka-Goetz
Published in: Developmental Cell, Issue 51/6, 2019, Page(s) 698-712.e8, ISSN 1534-5807
Publisher: Cell Press
DOI: 10.1016/j.devcel.2019.11.014

Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins

Author(s): Laurens Lindenburg, Tuomas Huovinen, Kayleigh van de Wiel, Michael Herger, Michael R Snaith, Florian Hollfelder
Published in: Nucleic Acids Research, Issue 48/11, 2020, Page(s) e63-e63, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkaa270

Deep learning guided image-based droplet sorting for on-demand selection and analysis of single cells and 3D cell cultures

Author(s): Vasileios Anagnostidis, Benjamin Sherlock, Jeremy Metz, Philip Mair, Florian Hollfelder, Fabrice Gielen
Published in: Lab on a Chip, Issue 20/5, 2020, Page(s) 889-900, ISSN 1473-0197
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0lc00055h

Global fitness landscapes of the Shine-Dalgarno sequence

Author(s): Syue-Ting Kuo, Ruey-Lin Jahn, Yuan-Ju Cheng, Yi-Lan Chen, Yun-Ju Lee, Florian Hollfelder, Jin-Der Wen, Hsin-Hung David Chou
Published in: Genome Research, Issue 30/5, 2020, Page(s) 711-723, ISSN 1088-9051
Publisher: Cold Spring Harbor Laboratory Press
DOI: 10.1101/gr.260182.119

Microfluidic platform for live cell imaging of 3D cultures with clone retrieval

Author(s): Carla Mulas; Andrew C Hodgson; Timo N Kohler; Chibeza C. Agley; Florian Hollfelder; Austin Smith; Austin Smith; Kevin J. Chalut
Published in: Lab Chip, Issue 9, 2020, Page(s) 2580-2591, ISSN 1473-0197
Publisher: Royal Society of Chemistry
DOI: 10.1101/2020.02.17.952689

Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays

Author(s): Tuomas Huovinen, Laurens Lindenburg, Ralph Minter, Florian Hollfelder
Published in: Analytical Chemistry, Issue 93/4, 2021, Page(s) 2166-2173, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.0c03992

Investigating host-microbiome interactions by droplet based microfluidics

Author(s): Alexandra S. Tauzin, Mariana Rangel Pereira, Liisa D. Van Vliet, Pierre-Yves Colin, Elisabeth Laville, Jeremy Esque, Sandrine Laguerre, Bernard Henrissat, Nicolas Terrapon, Vincent Lombard, Marion Leclerc, Joël Doré, Florian Hollfelder, Gabrielle Potocki-Veronese
Published in: Microbiome, Issue 8/1, 2020, Page(s) 141, ISSN 2049-2618
Publisher: BMC Spinger Nature
DOI: 10.1186/s40168-020-00911-z

Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies

Author(s): Elliot J. Medcalf, Maximilian Gantz, Tomasz S. Kaminski*, and Florian Hollfelder*
Published in: Analytical Chemistry, 2023, Page(s) 4597–4604, ISSN 1608-3199
Publisher: Nature Publishing
DOI: 10.1021/acs.analchem.2c04144

Gigavalent Display of Proteins on Monodisperse Polyacrylamide Hydrogels as a Versatile Modular Platform for Functional Assays and Protein Engineering

Author(s): Thomas Fryer, Joel David Rogers, Christopher Mellor, Timo N. Kohler, Ralph Minter, Florian Hollfelder
Published in: ACS Central Science, 2022, Page(s) 1182–1195, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/acscentsci.2c00576

Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance.

Author(s): Richter, Esther S; Link, Andreas; McGrath, John S; Sparrow, Raymond W; Gantz, Maximilian; Medcalf, Elliot J; Hollfelder, Florian; Franke, Thomas
Published in: Lab Chip, Issue 6, 2023, Page(s) 195-202, ISSN 1473-0189
Publisher: Royal society of Chemistry
DOI: 10.17863/cam.91468

Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro

Author(s): Gianluca Amadei, Kasey Y.C. Lau, Joachim De Jonghe, Carlos W. Gantner, Berna Sozen, Christopher Chan, Meng Zhu, Christos Kyprianou, Florian Hollfelder, Magdalena Zernicka-Goetz
Published in: Developmental Cell, Issue 56/3, 2021, Page(s) 366-382.e9, ISSN 1534-5807
Publisher: Cell Press
DOI: 10.1016/j.devcel.2020.12.004

Specificity Effects of Amino Acid Substitutions in Promiscuous Hydrolases: Context-Dependence of Catalytic Residue Contributions to Local Fitness Landscapes in Nearby Sequence Space

Author(s): Christopher D. Bayer, Bert van Loo, Florian Hollfelder
Published in: ChemBioChem, Issue 18/11, 2017, Page(s) 1001-1015, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201600657

Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity

Author(s): Miriam Kaltenbach, Stephane Emond, Florian Hollfelder, Nobuhiko Tokuriki
Published in: PLOS Genetics, Issue 12/10, 2016, Page(s) e1006305, ISSN 1553-7404
Publisher: PLOS
DOI: 10.1371/journal.pgen.1006305

Structural and Mechanistic Analysis of the Choline Sulfatase from Sinorhizobium melliloti : A Class I Sulfatase Specific for an Alkyl Sulfate Ester

Author(s): Bert van Loo, Markus Schober, Eugene Valkov, Magdalena Heberlein, Erich Bornberg-Bauer, Kurt Faber, Marko Hyvönen, Florian Hollfelder
Published in: Journal of Molecular Biology, Issue 430/7, 2018, Page(s) 1004-1023, ISSN 0022-2836
Publisher: Academic Press
DOI: 10.1016/j.jmb.2018.02.010

Divergent synthesis of biflavonoids yields novel inhibitors of the aggregation of amyloid β (1–42)

Author(s): Tze Han Sum, Tze Jing Sum, Súil Collins, Warren R. J. D. Galloway, David G. Twigg, Florian Hollfelder, David R. Spring
Published in: Organic & Biomolecular Chemistry, Issue 15/21, 2017, Page(s) 4554-4570, ISSN 1477-0520
Publisher: Royal Society of Chemistry
DOI: 10.1039/C7OB00804J

Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

Author(s): Fabrice Gielen, Raphaelle Hours, Stephane Emond, Martin Fischlechner, Ursula Schell, Florian Hollfelder
Published in: Proceedings of the National Academy of Sciences, Issue 113/47, 2016, Page(s) E7383-E7389, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1606927113

Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset

Author(s): Charlotte M. Miton, Stefanie Jonas, Gerhard Fischer, Fernanda Duarte, Mark F. Mohamed, Bert van Loo, Bálint Kintses, Shina C. L. Kamerlin, Nobuhiko Tokuriki, Marko Hyvönen, Florian Hollfelder
Published in: Proceedings of the National Academy of Sciences, Issue 115/31, 2018, Page(s) E7293-E7302, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1607817115

Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets

Author(s): Fabrice Gielen, Maren Butz, Eric J. Rees, Miklos Erdelyi, Tommaso Moschetti, Marko Hyvönen, Joshua B. Edel, Clemens F. Kaminski, Florian Hollfelder
Published in: Analytical Chemistry, Issue 89/2, 2016, Page(s) 1092-1101, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.6b02528

Transition-State Interactions in a Promiscuous Enzyme: Sulfate and Phosphate Monoester Hydrolysis by Pseudomonas aeruginosa Arylsulfatase

Author(s): Bert van Loo, Ryan Berry, Usa Boonyuen, Mark F. Mohamed, Marko Golicnik, Alvan C. Hengge, Florian Hollfelder
Published in: Biochemistry, Issue 58/10, 2019, Page(s) 1363-1378, ISSN 0006-2960
Publisher: American Chemical Society
DOI: 10.1021/acs.biochem.8b00996

Balancing Specificity and Promiscuity in Enzyme Evolution: Multidimensional Activity Transitions in the Alkaline Phosphatase Superfamily

Author(s): Bert van Loo, Christopher D. Bayer, Gerhard Fischer, Stefanie Jonas, Eugene Valkov, Mark F. Mohamed, Anastassia Vorobieva, Celine Dutruel, Marko Hyvönen, Florian Hollfelder
Published in: Journal of the American Chemical Society, Issue 141/1, 2018, Page(s) 370-387, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.8b10290

Long‐Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation

Author(s): Hans Kleine‐Brüggeney, Liisa D. van Vliet, Carla Mulas, Fabrice Gielen, Chibeza C. Agley, José C. R. Silva, Austin Smith, Kevin Chalut, Florian Hollfelder
Published in: Small, Issue 15/5, 2019, Page(s) 1804576, ISSN 1613-6810
Publisher: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.201804576

Controlled oil/water partitioning of hydrophobic substrates extends the bioanalytical applications of droplet-based microfluidics

Author(s): Tomas Buryska, Michal Vasina, Fabrice Gielen, Pavel Vanacek, Liisa van Vliet, Jan Jezek, Zdenek Pilat, Pavel Zemanek, Jiří Damborský, Florian Hollfelder, Zbyněk Prokop
Published in: Analytical Chemistry, 2019, ISSN 0003-2700
Publisher: American Chemical Society
DOI: 10.1021/acs.analchem.9b01839

Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats

Author(s): Laurens H. Lindenburg, Teodors Pantelejevs, Fabrice Gielen, Pedro Zuazua-Villar, Maren Butz, Eric Rees, Clemens F. Kaminski, Jessica A. Downs, Marko Hyvönen, Florian Hollfelder
Published in: Proc Natl Acad Sci U S A ., 2021, Page(s) e2017708118, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2017708118

High-throughput total RNA sequencing in single cells using VASA-seq

Author(s): Fredrik Salmen, Joachim De Jonghe, Tomasz S. Kaminski, Anna Alemany, Guillermo E. Parada, Joe Verity-Legg, Ayaka Yanagida, Timo N. Kohler, Nicholas Battich, Floris van den Brekel, Anna L. Ellermann, Alfonso Martinez Arias, Jennifer Nichols, Martin Hemberg, Florian Hollfelder & Alexander van Oudenaarden
Published in: Nature Biotechnology, 2022, Page(s) 1780–1793, ISSN 1087-0156
Publisher: Nature Publishing Group
DOI: 10.1038/s41587-022-01361-8

Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis

Author(s): Stephane Emond, Maya Petek, Emily J. Kay, Brennen Heames, Sean R. A. Devenish, Nobuhiko Tokuriki, Florian Hollfelder
Published in: Nature Communications, Issue 11/1, 2020, Page(s) 1, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-020-17061-3

Functional metagenomic screening identifies an unexpected β-glucuronidase

Author(s): Stefanie Neun, Paul Brear, Eleanor Campbell, Theodora Tryfona, Kamel El Omari, Armin Wagner, Paul Dupree, Marko Hyvönen, Florian Hollfelder
Published in: Nature Chemical Biology, 2022, Page(s) 1096–1103, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1038/s41589-022-01071-x

Embryo model completes gastrulation to neurulation and organogenesis

Author(s): Gianluca Amadei, Charlotte E. Handford, Chengxiang Qiu, Joachim De Jonghe, Hannah Greenfeld, Martin Tran, Beth K. Martin, Dong-Yuan Chen, Alejandro Aguilera-Castrejon, Jacob H. Hanna, Michael B. Elowitz, Florian Hollfelder, Jay Shendure, David M. Glover & Magdalena Zernicka-Goetz
Published in: Nature, 2022, Page(s) 143–153, ISSN 1476-4687
Publisher: Nature
DOI: 10.1038/s41586-022-05246-3

Controlled Ligand Exchange Between Ruthenium Organometallic Cofactor Precursors and a Naïve Protein Scaffold Generates Artificial Metalloenzymes Catalysing Transfer Hydrogenation

Author(s): George S. Biggs, Oskar James Klein, Sarah L. Maslen, J. Mark Skehel, Trevor J. Rutherford, Stefan M. V. Freund, Florian Hollfelder, Sally R. Boss, Paul D. Barker
Published in: Angewandte Chemie International Edition, Issue 60/19, 2021, Page(s) 10919-10927, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202015834

In vitro evolution of antibody affinity via insertional scanning mutagenesis of an entire antibody variable region

Author(s): Kalliopi Skamaki, Stephane Emond, Matthieu Chodorge, John Andrews, D. Gareth Rees, Daniel Cannon, Bojana Popovic, Andrew Buchanan, Ralph R. Minter, Florian Hollfelder
Published in: Proceedings of the National Academy of Sciences, Issue 117/44, 2020, Page(s) 27307-27318, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.2002954117

Agarose microgel culture delineates lumenogenesis in naive and primed human pluripotent stem cells

Author(s): Magdalena Schindler, Dylan Siriwardena, Timo N. Kohler, Anna L. Ellermann, Erin Slatery, Clara Munger, Florian Hollfelder, Thorsten E. Boroviak
Published in: Stem Cell Reports, Issue 16/5, 2021, Page(s) 1347-1362, ISSN 2213-6711
Publisher: Cell Press
DOI: 10.1016/j.stemcr.2021.04.009

Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase interactions with substrates

Author(s): Remkes A. Scheele, Laurens H. Lindenburg, Maya Petek, Markus Schober, Kevin N. Dalby, Florian Hollfelder
Published in: Nature Communications, 2022, Page(s) 844, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-28396-4

Ultrahigh-Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay

Author(s): Miriam Klaus,Paul Jannis Zurek, Tomasz S. Kaminski, Ahir Pushpanath, Katharina Neufeld, Florian Hollfelder
Published in: ChemBioChem, 2021, Page(s) 3292-3299, ISSN 1439-7633
Publisher: Wiley
DOI: 10.1002/cbic.202100322

Spatial profiling of early primate gastrulation in utero

Author(s): Sophie Bergmann, Christopher A. Penfold, Erin Slatery, Dylan Siriwardena, Charis Drummer, Stephen Clark, Stanley E. Strawbridge, Keiko Kishimoto, Alice Vickers, Mukul Tewary, Timo N. Kohler, Florian Hollfelder, Wolf Reik, Erika Sasaki, Rüdiger Behr & Thorsten E. Boroviak
Published in: Nature, 2022, Page(s) 136–143, ISSN 1476-4687
Publisher: Nature
DOI: 10.1038/s41586-022-04953-1

Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation

Author(s): Lucía Cordero-Espinoza, Anna M. Dowbaj, Timo N. Kohler, Bernhard Strauss, Olga Sarlidou, German Belenguer, Clare Pacini, Nuno P. Martins, Ross Dobie, John R. Wilson-Kanamori, Richard Butler, Nicole Prior, Palle Serup, Florian Jug, Neil C. Henderson, Florian Hollfelder, Meritxell Huch
Published in: Cell Stem Cell, 2021, ISSN 1934-5909
Publisher: Cell Press
DOI: 10.1016/j.stem.2021.07.002

Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension

Author(s): Min Bao, Jake Cornwall-Scoones, Estefania Sanchez-Vasquez, Dong-Yuan Chen, Joachim De Jonghe, Shahriar Shadkhoo, Florian Hollfelder, Matt Thomson, David M. Glover & Magdalena Zernicka-Goetz
Published in: Nature Cell Biology, 2022, Page(s) 1341–1349, ISSN 1465-7392
Publisher: Nature Publishing Group
DOI: 10.1038/s41556-022-00984-y

High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform

Author(s): Stefanie Neun, Liisa van Vliet, Florian Hollfelder, and Fabrice Gielen
Published in: Analytical Chemistry, 2022, Page(s) 16701–16710, ISSN 1608-3199
Publisher: ACS
DOI: 10.1021/acs.analchem.2c03164

Growth amplification in ultrahigh-throughput microdroplet screening increases sensitivity of clonal enzyme assays and minimizes phenotypic variation

Author(s): Paul Jannis Zurek, Raphaëlle Hours, Ursula Schell, Ahir Pushpanath, Florian Hollfelder
Published in: Lab on a Chip, Issue 21/1, 2021, Page(s) 163-173, ISSN 1473-0197
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0lc00830c

spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content

Author(s): Joachim De Jonghe, Tomasz S. Kaminski, David B. Morse, Marcin Tabaka, Anna L. Ellermann, Timo N. Kohler, Gianluca Amadei, Charlotte Handford, Gregory M. Findlay, Magdalena Zernicka-Goetz, Sarah A. Teichmann, Florian Hollfelder
Published in: bioRxiv, 2023, Page(s) n/a, ISSN 2692-8205
Publisher: CSH Press
DOI: 10.1101/2023.01.12.523500

Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies

Author(s): Katrin Fischer, Aleksei Lulla, Tsz Y So, Pehuén Pereyra-Gerber, Matthew I. J. Raybould, Timo N. Kohler, Tomasz S. Kaminski, Juan Carlos Yam-Puc, Robert Hughes, Florian Leiß-Maier, Paul Brear, Nicholas J. Matheson, Charlotte M. Deane, Marko Hyvönen, James E. D. Thaventhiran, Florian Hollfelder
Published in: bioRxiv, 2023, Page(s) n/a, ISSN 2692-8205
Publisher: Cold Spring Harbour Press
DOI: 10.1101/2023.01.10.523494

Selection of a Promiscuous Minimalist cAMP Phosphodiesterase from a Library of De Novo Designed Proteins

Author(s): J. David Schnettler, Michael S. Wang, Maximilian Gantz, Christina Karas, Florian Hollfelder, Michael H. Hecht
Published in: BioRxiv, 2023, Page(s) n/a, ISSN 2692-8205
Publisher: CSH Press
DOI: 10.1101/2023.02.13.528392

Versatile Product Detection via Coupled Assays for Ultra-high-throughput Screening of Carbohydrate-Active-Enzymes in Microfluidic Droplets

Author(s): Simon Ladeveze, Paul J. Zurek, Tomasz S. Kaminski, Stephane Emond, Florian Hollfelder
Published in: Biorxiv, 2023, Page(s) n/a, ISSN 2692-8205
Publisher: CDH Laboratory
DOI: 10.1101/2023.03.29.534725

High-Throughput, Lysis-Free Screening for Sulfatase Activity Using Escherichia coli Autodisplay in Microdroplets

Author(s): Bert van Loo, Magdalena Heberlein, Philip Mair, Anastasia Zinchenko, Jan Schüürmann, Bernard D. G. Eenink, Josephin M. Holstein, Carina Dilkaute, Joachim Jose, Florian Hollfelder, Erich Bornberg-Bauer
Published in: ACS Synthetic Biology, Issue 8/12, 2019, Page(s) 2690-2700, ISSN 2161-5063
Publisher: American Chemical Society
DOI: 10.1021/acssynbio.9b00274

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MODULAR MICROFLUIDIC DEVICES, SYSTEMS AND METHODS FOR TOTAL RNA ANALYSES

Application/Publication number: 20 21052112
Date: 2021-08-13

MODULAR MICROFLUIDIC METHODS AND DEVICES

Application/Publication number: 20 21052111
Date: 2021-08-13

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