Exploring the Chemical Biology of Sequence Space via Picoliter Droplets

Publicaciones

A Titratable Cell Lysis-on-Demand System for Droplet-Compartmentalized Ultrahigh-Throughput Screening in Functional Metagenomics and Directed Evolution

Autores: Che Fai Alex Wong, Liisa van Vliet, Swapnil Vilas Bhujbal, Chengzhi Guo, Marit Sletmoen, Bjørn Torger Stokke, Florian Hollfelder, Rahmi Lale
Publicado en: ACS Synthetic Biology, 2021, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.1c00084

Ultrahigh-Throughput Directed Evolution of a Metal-Free α/β-Hydrolase with a Cys-His-Asp Triad into an Efficient Phosphotriesterase

Autores: J. David Schnettler, Oskar James Klein, Tomasz S. Kaminski, Pierre-Yves Colin, Florian Hollfelder
Publicado en: J Am Chem Soc, 2023, Página(s) 1083–1096, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.2c10673

Ultrahigh-Throughput Enzyme Engineering and Discovery in In Vitro Compartments

Autores: Maximilian Gantz, Stefanie Neun, Elliot J. Medcalf, Liisa D. van Vliet, Florian Hollfelder
Publicado en: Chemical Reviews, 2023, Página(s) ASAP, ISSN 0009-2665
Editor: American Chemical Society
DOI: 10.1021/acs.chemrev.2c00910

Microgel culture and spatial identity mapping elucidate the signalling requirements for primate epiblast and amnion formation

Autores: Clara Munger, Timo N. Kohler, Erin Slatery, Anna L. Ellermann, Sophie Bergmann, Christopher A. Penfold, Ioakeim Ampartzidis, Yutong Chen, Florian Hollfelder, Thorsten E. Boroviak
Publicado en: Development, 2022, Página(s) dev200263, ISSN 1477-9129
Editor: The company of biologists
DOI: 10.1242/dev.200263

UMI-linked consensus sequencing enables phylogenetic analysis of directed evolution

Autores: Paul Jannis Zurek, Philipp Knyphausen, Katharina Neufeld, Ahir Pushpanath, Florian Hollfelder
Publicado en: Nature Communications, Edición 11/1, 2020, Página(s) 6023, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-020-19687-9

Enzymatic N-Allylation of Primary and Secondary Amines Using Renewable Cinnamic Acids Enabled by Bacterial Reductive Aminases

Autores: Godwin A. Aleku, Gabriel R. Titchiner, George W. Roberts, Sasha R. Derrington, James R. Marshall, Florian Hollfelder, Nicholas J. Turner, and David Leys
Publicado en: ACS Sustainable Chem. Eng., Edición 2168-0485, 2022, Página(s) 6794–6806, ISSN 2168-0485
Editor: American Chemical Society
DOI: 10.1021/acssuschemeng.2c01180

Evolution of protease activation and specificity via alpha-2-macroglobulin-mediated covalent capture.

Autores: Knyphausen P, Rangel Pereira M, Brear P, Hyvönen M, Jermutus L, Hollfelder F.
Publicado en: Nature Communications, Edición 14, 2023, Página(s) 768, ISSN 1608-3199
Editor: Nature
DOI: 10.1038/s41467-023-36099-7

Engineering the protein dynamics of an ancestral luciferase

Autores: Andrea Schenkmayerova, Gaspar P. Pinto, Martin Toul, Martin Marek, Lenka Hernychova, Joan Planas-Iglesias, Veronika Daniel Liskova, Daniel Pluskal, Michal Vasina, Stephane Emond, Mark Dörr, Radka Chaloupkova, David Bednar, Zbynek Prokop, Florian Hollfelder, Uwe T. Bornscheuer, Jiri Damborsky
Publicado en: Nature Communications, Edición 12/1, 2021, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-021-23450-z

Self-Organization of Mouse Stem Cells into an Extended Potential Blastoid

Autores: Berna Sozen, Andy L. Cox, Joachim De Jonghe, Min Bao, Florian Hollfelder, David M. Glover, Magdalena Zernicka-Goetz
Publicado en: Developmental Cell, Edición 51/6, 2019, Página(s) 698-712.e8, ISSN 1534-5807
Editor: Cell Press
DOI: 10.1016/j.devcel.2019.11.014

Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins

Autores: Laurens Lindenburg, Tuomas Huovinen, Kayleigh van de Wiel, Michael Herger, Michael R Snaith, Florian Hollfelder
Publicado en: Nucleic Acids Research, Edición 48/11, 2020, Página(s) e63-e63, ISSN 0305-1048
Editor: Oxford University Press
DOI: 10.1093/nar/gkaa270

Deep learning guided image-based droplet sorting for on-demand selection and analysis of single cells and 3D cell cultures

Autores: Vasileios Anagnostidis, Benjamin Sherlock, Jeremy Metz, Philip Mair, Florian Hollfelder, Fabrice Gielen
Publicado en: Lab on a Chip, Edición 20/5, 2020, Página(s) 889-900, ISSN 1473-0197
Editor: Royal Society of Chemistry
DOI: 10.1039/d0lc00055h

Global fitness landscapes of the Shine-Dalgarno sequence

Autores: Syue-Ting Kuo, Ruey-Lin Jahn, Yuan-Ju Cheng, Yi-Lan Chen, Yun-Ju Lee, Florian Hollfelder, Jin-Der Wen, Hsin-Hung David Chou
Publicado en: Genome Research, Edición 30/5, 2020, Página(s) 711-723, ISSN 1088-9051
Editor: Cold Spring Harbor Laboratory Press
DOI: 10.1101/gr.260182.119

Microfluidic platform for live cell imaging of 3D cultures with clone retrieval

Autores: Carla Mulas; Andrew C Hodgson; Timo N Kohler; Chibeza C. Agley; Florian Hollfelder; Austin Smith; Austin Smith; Kevin J. Chalut
Publicado en: Lab Chip, Edición 9, 2020, Página(s) 2580-2591, ISSN 1473-0197
Editor: Royal Society of Chemistry
DOI: 10.1101/2020.02.17.952689

Multiplexed Affinity Characterization of Protein Binders Directly from a Crude Cell Lysate by Covalent Capture on Suspension Bead Arrays

Autores: Tuomas Huovinen, Laurens Lindenburg, Ralph Minter, Florian Hollfelder
Publicado en: Analytical Chemistry, Edición 93/4, 2021, Página(s) 2166-2173, ISSN 0003-2700
Editor: American Chemical Society
DOI: 10.1021/acs.analchem.0c03992

Investigating host-microbiome interactions by droplet based microfluidics

Autores: Alexandra S. Tauzin, Mariana Rangel Pereira, Liisa D. Van Vliet, Pierre-Yves Colin, Elisabeth Laville, Jeremy Esque, Sandrine Laguerre, Bernard Henrissat, Nicolas Terrapon, Vincent Lombard, Marion Leclerc, Joël Doré, Florian Hollfelder, Gabrielle Potocki-Veronese
Publicado en: Microbiome, Edición 8/1, 2020, Página(s) 141, ISSN 2049-2618
Editor: BMC Spinger Nature
DOI: 10.1186/s40168-020-00911-z

Ultra-High-Throughput Absorbance-Activated Droplet Sorting for Enzyme Screening at Kilohertz Frequencies

Autores: Elliot J. Medcalf, Maximilian Gantz, Tomasz S. Kaminski*, and Florian Hollfelder*
Publicado en: Analytical Chemistry, 2023, Página(s) 4597–4604, ISSN 1608-3199
Editor: Nature Publishing
DOI: 10.1021/acs.analchem.2c04144

Gigavalent Display of Proteins on Monodisperse Polyacrylamide Hydrogels as a Versatile Modular Platform for Functional Assays and Protein Engineering

Autores: Thomas Fryer, Joel David Rogers, Christopher Mellor, Timo N. Kohler, Ralph Minter, Florian Hollfelder
Publicado en: ACS Central Science, 2022, Página(s) 1182–1195, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/acscentsci.2c00576

Acoustic sorting of microfluidic droplets at kHz rates using optical absorbance.

Autores: Richter, Esther S; Link, Andreas; McGrath, John S; Sparrow, Raymond W; Gantz, Maximilian; Medcalf, Elliot J; Hollfelder, Florian; Franke, Thomas
Publicado en: Lab Chip, Edición 6, 2023, Página(s) 195-202, ISSN 1473-0189
Editor: Royal society of Chemistry
DOI: 10.17863/cam.91468

Inducible Stem-Cell-Derived Embryos Capture Mouse Morphogenetic Events In Vitro

Autores: Gianluca Amadei, Kasey Y.C. Lau, Joachim De Jonghe, Carlos W. Gantner, Berna Sozen, Christopher Chan, Meng Zhu, Christos Kyprianou, Florian Hollfelder, Magdalena Zernicka-Goetz
Publicado en: Developmental Cell, Edición 56/3, 2021, Página(s) 366-382.e9, ISSN 1534-5807
Editor: Cell Press
DOI: 10.1016/j.devcel.2020.12.004

Specificity Effects of Amino Acid Substitutions in Promiscuous Hydrolases: Context-Dependence of Catalytic Residue Contributions to Local Fitness Landscapes in Nearby Sequence Space

Autores: Christopher D. Bayer, Bert van Loo, Florian Hollfelder
Publicado en: ChemBioChem, Edición 18/11, 2017, Página(s) 1001-1015, ISSN 1439-4227
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201600657

Functional Trade-Offs in Promiscuous Enzymes Cannot Be Explained by Intrinsic Mutational Robustness of the Native Activity

Autores: Miriam Kaltenbach, Stephane Emond, Florian Hollfelder, Nobuhiko Tokuriki
Publicado en: PLOS Genetics, Edición 12/10, 2016, Página(s) e1006305, ISSN 1553-7404
Editor: PLOS
DOI: 10.1371/journal.pgen.1006305

Structural and Mechanistic Analysis of the Choline Sulfatase from Sinorhizobium melliloti : A Class I Sulfatase Specific for an Alkyl Sulfate Ester

Autores: Bert van Loo, Markus Schober, Eugene Valkov, Magdalena Heberlein, Erich Bornberg-Bauer, Kurt Faber, Marko Hyvönen, Florian Hollfelder
Publicado en: Journal of Molecular Biology, Edición 430/7, 2018, Página(s) 1004-1023, ISSN 0022-2836
Editor: Academic Press
DOI: 10.1016/j.jmb.2018.02.010

Divergent synthesis of biflavonoids yields novel inhibitors of the aggregation of amyloid β (1–42)

Autores: Tze Han Sum, Tze Jing Sum, Súil Collins, Warren R. J. D. Galloway, David G. Twigg, Florian Hollfelder, David R. Spring
Publicado en: Organic & Biomolecular Chemistry, Edición 15/21, 2017, Página(s) 4554-4570, ISSN 1477-0520
Editor: Royal Society of Chemistry
DOI: 10.1039/C7OB00804J

Ultrahigh-throughput–directed enzyme evolution by absorbance-activated droplet sorting (AADS)

Autores: Fabrice Gielen, Raphaelle Hours, Stephane Emond, Martin Fischlechner, Ursula Schell, Florian Hollfelder
Publicado en: Proceedings of the National Academy of Sciences, Edición 113/47, 2016, Página(s) E7383-E7389, ISSN 0027-8424
Editor: National Academy of Sciences
DOI: 10.1073/pnas.1606927113

Evolutionary repurposing of a sulfatase: A new Michaelis complex leads to efficient transition state charge offset

Autores: Charlotte M. Miton, Stefanie Jonas, Gerhard Fischer, Fernanda Duarte, Mark F. Mohamed, Bert van Loo, Bálint Kintses, Shina C. L. Kamerlin, Nobuhiko Tokuriki, Marko Hyvönen, Florian Hollfelder
Publicado en: Proceedings of the National Academy of Sciences, Edición 115/31, 2018, Página(s) E7293-E7302, ISSN 0027-8424
Editor: National Academy of Sciences
DOI: 10.1073/pnas.1607817115

Quantitative Affinity Determination by Fluorescence Anisotropy Measurements of Individual Nanoliter Droplets

Autores: Fabrice Gielen, Maren Butz, Eric J. Rees, Miklos Erdelyi, Tommaso Moschetti, Marko Hyvönen, Joshua B. Edel, Clemens F. Kaminski, Florian Hollfelder
Publicado en: Analytical Chemistry, Edición 89/2, 2016, Página(s) 1092-1101, ISSN 0003-2700
Editor: American Chemical Society
DOI: 10.1021/acs.analchem.6b02528

Transition-State Interactions in a Promiscuous Enzyme: Sulfate and Phosphate Monoester Hydrolysis by Pseudomonas aeruginosa Arylsulfatase

Autores: Bert van Loo, Ryan Berry, Usa Boonyuen, Mark F. Mohamed, Marko Golicnik, Alvan C. Hengge, Florian Hollfelder
Publicado en: Biochemistry, Edición 58/10, 2019, Página(s) 1363-1378, ISSN 0006-2960
Editor: American Chemical Society
DOI: 10.1021/acs.biochem.8b00996

Balancing Specificity and Promiscuity in Enzyme Evolution: Multidimensional Activity Transitions in the Alkaline Phosphatase Superfamily

Autores: Bert van Loo, Christopher D. Bayer, Gerhard Fischer, Stefanie Jonas, Eugene Valkov, Mark F. Mohamed, Anastassia Vorobieva, Celine Dutruel, Marko Hyvönen, Florian Hollfelder
Publicado en: Journal of the American Chemical Society, Edición 141/1, 2018, Página(s) 370-387, ISSN 0002-7863
Editor: American Chemical Society
DOI: 10.1021/jacs.8b10290

Long‐Term Perfusion Culture of Monoclonal Embryonic Stem Cells in 3D Hydrogel Beads for Continuous Optical Analysis of Differentiation

Autores: Hans Kleine‐Brüggeney, Liisa D. van Vliet, Carla Mulas, Fabrice Gielen, Chibeza C. Agley, José C. R. Silva, Austin Smith, Kevin Chalut, Florian Hollfelder
Publicado en: Small, Edición 15/5, 2019, Página(s) 1804576, ISSN 1613-6810
Editor: Wiley - V C H Verlag GmbbH & Co.
DOI: 10.1002/smll.201804576

Controlled oil/water partitioning of hydrophobic substrates extends the bioanalytical applications of droplet-based microfluidics

Autores: Tomas Buryska, Michal Vasina, Fabrice Gielen, Pavel Vanacek, Liisa van Vliet, Jan Jezek, Zdenek Pilat, Pavel Zemanek, Jiří Damborský, Florian Hollfelder, Zbyněk Prokop
Publicado en: Analytical Chemistry, 2019, ISSN 0003-2700
Editor: American Chemical Society
DOI: 10.1021/acs.analchem.9b01839

Improved RAD51 binders through motif shuffling based on the modularity of BRC repeats

Autores: Laurens H. Lindenburg, Teodors Pantelejevs, Fabrice Gielen, Pedro Zuazua-Villar, Maren Butz, Eric Rees, Clemens F. Kaminski, Jessica A. Downs, Marko Hyvönen, Florian Hollfelder
Publicado en: Proc Natl Acad Sci U S A ., 2021, Página(s) e2017708118, ISSN 0027-8424
Editor: National Academy of Sciences
DOI: 10.1073/pnas.2017708118

High-throughput total RNA sequencing in single cells using VASA-seq

Autores: Fredrik Salmen, Joachim De Jonghe, Tomasz S. Kaminski, Anna Alemany, Guillermo E. Parada, Joe Verity-Legg, Ayaka Yanagida, Timo N. Kohler, Nicholas Battich, Floris van den Brekel, Anna L. Ellermann, Alfonso Martinez Arias, Jennifer Nichols, Martin Hemberg, Florian Hollfelder & Alexander van Oudenaarden
Publicado en: Nature Biotechnology, 2022, Página(s) 1780–1793, ISSN 1087-0156
Editor: Nature Publishing Group
DOI: 10.1038/s41587-022-01361-8

Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis

Autores: Stephane Emond, Maya Petek, Emily J. Kay, Brennen Heames, Sean R. A. Devenish, Nobuhiko Tokuriki, Florian Hollfelder
Publicado en: Nature Communications, Edición 11/1, 2020, Página(s) 1, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-020-17061-3

Functional metagenomic screening identifies an unexpected β-glucuronidase

Autores: Stefanie Neun, Paul Brear, Eleanor Campbell, Theodora Tryfona, Kamel El Omari, Armin Wagner, Paul Dupree, Marko Hyvönen, Florian Hollfelder
Publicado en: Nature Chemical Biology, 2022, Página(s) 1096–1103, ISSN 1552-4450
Editor: Nature Publishing Group
DOI: 10.1038/s41589-022-01071-x

Embryo model completes gastrulation to neurulation and organogenesis

Autores: Gianluca Amadei, Charlotte E. Handford, Chengxiang Qiu, Joachim De Jonghe, Hannah Greenfeld, Martin Tran, Beth K. Martin, Dong-Yuan Chen, Alejandro Aguilera-Castrejon, Jacob H. Hanna, Michael B. Elowitz, Florian Hollfelder, Jay Shendure, David M. Glover & Magdalena Zernicka-Goetz
Publicado en: Nature, 2022, Página(s) 143–153, ISSN 1476-4687
Editor: Nature
DOI: 10.1038/s41586-022-05246-3

Controlled Ligand Exchange Between Ruthenium Organometallic Cofactor Precursors and a Naïve Protein Scaffold Generates Artificial Metalloenzymes Catalysing Transfer Hydrogenation

Autores: George S. Biggs, Oskar James Klein, Sarah L. Maslen, J. Mark Skehel, Trevor J. Rutherford, Stefan M. V. Freund, Florian Hollfelder, Sally R. Boss, Paul D. Barker
Publicado en: Angewandte Chemie International Edition, Edición 60/19, 2021, Página(s) 10919-10927, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202015834

In vitro evolution of antibody affinity via insertional scanning mutagenesis of an entire antibody variable region

Autores: Kalliopi Skamaki, Stephane Emond, Matthieu Chodorge, John Andrews, D. Gareth Rees, Daniel Cannon, Bojana Popovic, Andrew Buchanan, Ralph R. Minter, Florian Hollfelder
Publicado en: Proceedings of the National Academy of Sciences, Edición 117/44, 2020, Página(s) 27307-27318, ISSN 0027-8424
Editor: National Academy of Sciences
DOI: 10.1073/pnas.2002954117

Agarose microgel culture delineates lumenogenesis in naive and primed human pluripotent stem cells

Autores: Magdalena Schindler, Dylan Siriwardena, Timo N. Kohler, Anna L. Ellermann, Erin Slatery, Clara Munger, Florian Hollfelder, Thorsten E. Boroviak
Publicado en: Stem Cell Reports, Edición 16/5, 2021, Página(s) 1347-1362, ISSN 2213-6711
Editor: Cell Press
DOI: 10.1016/j.stemcr.2021.04.009

Droplet-based screening of phosphate transfer catalysis reveals how epistasis shapes MAP kinase interactions with substrates

Autores: Remkes A. Scheele, Laurens H. Lindenburg, Maya Petek, Markus Schober, Kevin N. Dalby, Florian Hollfelder
Publicado en: Nature Communications, 2022, Página(s) 844, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-022-28396-4

Ultrahigh-Throughput Detection of Enzymatic Alcohol Dehydrogenase Activity in Microfluidic Droplets with a Direct Fluorogenic Assay

Autores: Miriam Klaus,Paul Jannis Zurek, Tomasz S. Kaminski, Ahir Pushpanath, Katharina Neufeld, Florian Hollfelder
Publicado en: ChemBioChem, 2021, Página(s) 3292-3299, ISSN 1439-7633
Editor: Wiley
DOI: 10.1002/cbic.202100322

Spatial profiling of early primate gastrulation in utero

Autores: Sophie Bergmann, Christopher A. Penfold, Erin Slatery, Dylan Siriwardena, Charis Drummer, Stephen Clark, Stanley E. Strawbridge, Keiko Kishimoto, Alice Vickers, Mukul Tewary, Timo N. Kohler, Florian Hollfelder, Wolf Reik, Erika Sasaki, Rüdiger Behr & Thorsten E. Boroviak
Publicado en: Nature, 2022, Página(s) 136–143, ISSN 1476-4687
Editor: Nature
DOI: 10.1038/s41586-022-04953-1

Dynamic cell contacts between periportal mesenchyme and ductal epithelium act as a rheostat for liver cell proliferation

Autores: Lucía Cordero-Espinoza, Anna M. Dowbaj, Timo N. Kohler, Bernhard Strauss, Olga Sarlidou, German Belenguer, Clare Pacini, Nuno P. Martins, Ross Dobie, John R. Wilson-Kanamori, Richard Butler, Nicole Prior, Palle Serup, Florian Jug, Neil C. Henderson, Florian Hollfelder, Meritxell Huch
Publicado en: Cell Stem Cell, 2021, ISSN 1934-5909
Editor: Cell Press
DOI: 10.1016/j.stem.2021.07.002

Stem cell-derived synthetic embryos self-assemble by exploiting cadherin codes and cortical tension

Autores: Min Bao, Jake Cornwall-Scoones, Estefania Sanchez-Vasquez, Dong-Yuan Chen, Joachim De Jonghe, Shahriar Shadkhoo, Florian Hollfelder, Matt Thomson, David M. Glover & Magdalena Zernicka-Goetz
Publicado en: Nature Cell Biology, 2022, Página(s) 1341–1349, ISSN 1465-7392
Editor: Nature Publishing Group
DOI: 10.1038/s41556-022-00984-y

High-Throughput Steady-State Enzyme Kinetics Measured in a Parallel Droplet Generation and Absorbance Detection Platform

Autores: Stefanie Neun, Liisa van Vliet, Florian Hollfelder, and Fabrice Gielen
Publicado en: Analytical Chemistry, 2022, Página(s) 16701–16710, ISSN 1608-3199
Editor: ACS
DOI: 10.1021/acs.analchem.2c03164

Growth amplification in ultrahigh-throughput microdroplet screening increases sensitivity of clonal enzyme assays and minimizes phenotypic variation

Autores: Paul Jannis Zurek, Raphaëlle Hours, Ursula Schell, Ahir Pushpanath, Florian Hollfelder
Publicado en: Lab on a Chip, Edición 21/1, 2021, Página(s) 163-173, ISSN 1473-0197
Editor: Royal Society of Chemistry
DOI: 10.1039/d0lc00830c

spinDrop: a droplet microfluidic platform to maximise single-cell sequencing information content

Autores: Joachim De Jonghe, Tomasz S. Kaminski, David B. Morse, Marcin Tabaka, Anna L. Ellermann, Timo N. Kohler, Gianluca Amadei, Charlotte Handford, Gregory M. Findlay, Magdalena Zernicka-Goetz, Sarah A. Teichmann, Florian Hollfelder
Publicado en: bioRxiv, 2023, Página(s) n/a, ISSN 2692-8205
Editor: CSH Press
DOI: 10.1101/2023.01.12.523500

Microfluidics-enabled fluorescence-activated cell sorting of single pathogen-specific antibody secreting cells for the rapid discovery of monoclonal antibodies

Autores: Katrin Fischer, Aleksei Lulla, Tsz Y So, Pehuén Pereyra-Gerber, Matthew I. J. Raybould, Timo N. Kohler, Tomasz S. Kaminski, Juan Carlos Yam-Puc, Robert Hughes, Florian Leiß-Maier, Paul Brear, Nicholas J. Matheson, Charlotte M. Deane, Marko Hyvönen, James E. D. Thaventhiran, Florian Hollfelder
Publicado en: bioRxiv, 2023, Página(s) n/a, ISSN 2692-8205
Editor: Cold Spring Harbour Press
DOI: 10.1101/2023.01.10.523494

Selection of a Promiscuous Minimalist cAMP Phosphodiesterase from a Library of De Novo Designed Proteins

Autores: J. David Schnettler, Michael S. Wang, Maximilian Gantz, Christina Karas, Florian Hollfelder, Michael H. Hecht
Publicado en: BioRxiv, 2023, Página(s) n/a, ISSN 2692-8205
Editor: CSH Press
DOI: 10.1101/2023.02.13.528392

Versatile Product Detection via Coupled Assays for Ultra-high-throughput Screening of Carbohydrate-Active-Enzymes in Microfluidic Droplets

Autores: Simon Ladeveze, Paul J. Zurek, Tomasz S. Kaminski, Stephane Emond, Florian Hollfelder
Publicado en: Biorxiv, 2023, Página(s) n/a, ISSN 2692-8205
Editor: CDH Laboratory
DOI: 10.1101/2023.03.29.534725

High-Throughput, Lysis-Free Screening for Sulfatase Activity Using Escherichia coli Autodisplay in Microdroplets

Autores: Bert van Loo, Magdalena Heberlein, Philip Mair, Anastasia Zinchenko, Jan Schüürmann, Bernard D. G. Eenink, Josephin M. Holstein, Carina Dilkaute, Joachim Jose, Florian Hollfelder, Erich Bornberg-Bauer
Publicado en: ACS Synthetic Biology, Edición 8/12, 2019, Página(s) 2690-2700, ISSN 2161-5063
Editor: American Chemical Society
DOI: 10.1021/acssynbio.9b00274

Derechos de propiedad intelectual

MODULAR MICROFLUIDIC DEVICES, SYSTEMS AND METHODS FOR TOTAL RNA ANALYSES

Número de solicitud/publicación: 20 21052112
Fecha: 2021-08-13

MODULAR MICROFLUIDIC METHODS AND DEVICES

Número de solicitud/publicación: 20 21052111
Fecha: 2021-08-13

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