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Hydrostatic pressure and prokaryotic activity in the deep sea

Objective

The main goal of this project is to understand deep-sea prokaryotic activity and metabolism under in situ hydrostatic pressure and reveal the contribution of piezophilic prokaryotes to the total prokaryotic community in the dark ocean. In the project, deep-sea prokaryotes will be incubated under in situ hydrostatic pressure with an in situ microbial incubator (ISMI) which has been developed in the lab of the applicant’s PhD supervisor in Japan. The ISMI, as the name suggests, is an instrument to incubate deep-sea biota at the depth of sampling and hence, without change of the hydrostatic pressure and temperature. Deep-sea prokaryotes will also be incubated under atmospheric pressure at otherwise identical conditions as a control, thus, this approach will reveal the impact of hydrostatic pressure on prokaryotic activity. Consequently, we will obtain information on the phylogeny and metabolism of piezophilic and piezotolerant prokaryotic populations in the dark ocean. The ISMI will be deployed during two long (ca. 1 month) research cruises, and a short (a week) cruise in the project. Prokaryotic heterotrophic and autotrophic production under in situ hydrostatic pressure will be measured, and piezophilic, piezotolerant, and piezosensitive prokaryotes will be studied with microautoradiography combined with catalyzed reporter deposition fluorescence in situ hybridization (MICRO-CARD-FISH) and omics approaches. Measuring metabolic activity of deep-sea microbes under in situ pressure conditions represents a major endeavor, and it remains unknown how large the fraction of piezophilic and piezotolerant prokaryotes in the deep ocean really is. The project has the potential to provide an answer to the question of the metabolic activity of microbes under in situ pressure conditions in the global ocean.

Fields of science (EuroSciVoc)

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Programme(s)

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Topic(s)

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Funding Scheme

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MSCA-IF-EF-ST - Standard EF

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Call for proposal

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(opens in new window) H2020-MSCA-IF-2015

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Coordinator

UNIVERSITAT WIEN
Net EU contribution

Net EU financial contribution. The sum of money that the participant receives, deducted by the EU contribution to its linked third party. It considers the distribution of the EU financial contribution between direct beneficiaries of the project and other types of participants, like third-party participants.

€ 178 156,80
Address
UNIVERSITATSRING 1
1010 WIEN
Austria

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Region
Ostösterreich Wien Wien
Activity type
Higher or Secondary Education Establishments
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Total cost

The total costs incurred by this organisation to participate in the project, including direct and indirect costs. This amount is a subset of the overall project budget.

€ 178 156,80
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