Landscape of epigenetic control in early and late germ line development

Información del proyecto

epiGERMetics

Identificador del acuerdo de subvención: 705939

DOI

10.3030/705939

Proyecto cerrado

Fecha de la firma de la CE 17 Febrero 2016

Fecha de inicio 1 Marzo 2016

Fecha de finalización 2 Octubre 2018

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 177 598,80

Aportación de la UE

€ 177 598,80

Coordinado por

STICHTING RADBOUD UNIVERSITEIT
Netherlands

Objetivo

Germ cells have unique role in development. They are the only cells required for the transmission of genetic material from generation to generation in sexually reproducing organisms. This makes them pivotal for the survival of species and ultimately for the propagation of life. Although germ cells undergo extensive cellular differentiation during gametogenesis, they are protected from somatic reprogramming. This requires precise control of transcriptional programs, misregulation of which leads to infertility and developmental defects. Moreover, germ cell-like properties acquired ectopically promote tumor formation. My aim is to understand how germ cell identity is controlled in vertebrates, using zebrafish as a genetic model. My hypothesis is that epigenetic control of gene expression is regulated in both the nucleus and cytoplasm of germ cells. In the nucleus, Polycomb Group (PcG) proteins regulate transcription by establishing repressive chromatin modifications. In the germ cytoplasm, translational regulation by Argonaute proteins, involved in RNA silencing pathways, is pivotal for maintaining germ cell identity. However, the events that facilitate cross-talk between the nucleus and germ cytoplasm are not known. Here, I propose to combine high-end developmental biology techniques with state-of-the art genomic technologies to investigate the cooperation between the PcG complex and Argonaute proteins during germ line development. I believe that the crossing point between my scientific interest (events outside the nucleus) and the expertise of my supervisor (regulation in the nucleus), creates a very solid background for this proposal. It will further develop my interdisciplinary skills and enhance my creative potential by allowing me to look at the regulation of germ cell identity from different angles. I am confident, that this proposal is the perfect stepping stone in becoming an independent scientist and an expert in the field of epigenetic gene regulation.

Ámbito científico (EuroSciVoc)

• ciencias naturales › ciencias biológicas › bioquímica › biomoléculas › proteínas
• ciencias naturales › ciencias biológicas › biología del desarrollo
• ciencias naturales › ciencias biológicas › genética › ARN

Palabras clave

• epigenetic
• germ line
• germ granules
• development
• zebrafish
• germ cells

Programa(s)

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions PROGRAMA PRINCIPAL
  
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
  

Tema(s)

• MSCA-IF-2015-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF)
  

Régimen de financiación

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

Convocatoria de propuestas

H2020-MSCA-IF-2015

Coordinador

STICHTING RADBOUD UNIVERSITEIT

• Aportación neta de la UEn: € 177 598,80
• Dirección: HOUTLAAN 4 ; 6525 XZ Nijmegen ; Países Bajos
• Región: Oost-Nederland › Gelderland › Arnhem/Nijmegen
• Tipo de actividad: Higher or Secondary Education Establishments
• Coste total: € 177 598,80