Adding Pieces to the Puzzle of Sexual Differentiation In P. falciparum: A Systematic Analysis of RNA Processing

Información del proyecto

SPARk

Identificador del acuerdo de subvención: 708056

DOI

10.3030/708056

Proyecto cerrado

Fecha de la firma de la CE 4 Marzo 2016

Fecha de inicio 1 Abril 2016

Fecha de finalización 31 Marzo 2018

Financiado con arreglo a

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Coste total

€ 171 460,80

Aportación de la UE

€ 171 460,80

Coordinado por

JUSTUS-LIEBIG-UNIVERSITAET GIESSEN
Germany

Objetivo

Malaria remains one of the most devastating diseases in modern times, with the heaviest mortality concentrated in young children living in resource-poor environments. Plasmodium falciparum causes the most severe form of malaria. The parasites have a complex life cycle, switching between a host and a mosquito vector. Transformation of a subset of parasites into specialized stages capable of sexual development – the gametocytes – is one of the most astonishing, yet not well understood, Plasmodium life cycle phases. As preparatory work for this proposal during my time at Harvard School of Public Health, I identified pathways specifically upregulated at the onset of sexual differentiation, an important gap in our knowledge of gametocytogenesis. Among these, four genes involved in different aspects of post-transcriptional gene regulation were highly upregulated. Studying this aspect of gametocyte biology can add to our understanding of how Plasmodium parasites orchestrate complex genetics switches and modulate the cellular response to developmental signals.
The aim of this proposal is to provide insight how these RNA-binding proteins contribute to sexual stage-specific gene expression. This project employs genetic approaches to investigate the role of these proteins during sexual development of P. falciparum. For that I will generate transgenic parasites and analyse the resulting phenotype in detail. Additionally, this study aims at characterisation the splicing activity of recombinantly produced proteins and to determine target RNAs in order to elucidate the underlying mechanism of RNA processing control.
This proposal can contribute to improved health conditions in malaria-endemic countries through a better understanding of malaria transmission and new approaches to treat the disease. Completion of the scientific project in combination with the proposed structured acquisition of professional skills will enable me to reintegrate into the European research community.

Ámbito científico (EuroSciVoc)

• ciencias médicas y de la salud › medicina básica › farmacología y farmacia › descubrimiento de fármacos
• ciencias médicas y de la salud › ciencias de la salud › enfermedad infecciosa › malaria
• ciencias médicas y de la salud › ciencias de la salud › salud pública y medio ambiental
• ciencias naturales › ciencias biológicas › bioquímica › biomoléculas › proteínas
• ciencias naturales › ciencias biológicas › genética › ARN

Palabras clave

• Malaria
• Gametocytes
• Transmission

Programa(s)

• H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions PROGRAMA PRINCIPAL
  
• H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility
  

Tema(s)

• MSCA-IF-2015-EF - Marie Skłodowska-Curie Individual Fellowships (IF-EF)
  

Régimen de financiación

MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF)

Convocatoria de propuestas

H2020-MSCA-IF-2015

Coordinador

JUSTUS-LIEBIG-UNIVERSITAET GIESSEN

• Aportación neta de la UEn: € 171 460,80
• Dirección: LUDWIGSTRASSE 23 ; 35390 GIESSEN ; Alemania
• Región: Hessen › Gießen › Gießen, Landkreis
• Tipo de actividad: Higher or Secondary Education Establishments
• Coste total: € 171 460,80