Visual genetics: establishment of a new discipline to visualize neuronal nuclear functions in real-time in intact nervous system by 4D Raman spectroscopy

Project Information

NEURAM

Grant agreement ID: 712821

DOI 10.3030/712821

Project closed

EC signature date 26 May 2016

Start date 1 October 2016

End date 30 November 2022

Funded under

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Total cost € 4 271 481,25

EU contribution € 4 271 481,25

Coordinated by

THE UNIVERSITY OF BIRMINGHAM
United Kingdom

Deliverables

Documents, reports (12)

Update Data Management Plan

Updates of the data management plan

Update Dissemination plan

Update of the Dissemination Plan

Action check meeting 1

Agenda of the Action check meeting 1 and presentations

Data Management Plan

Generation of Data Management Plan

Dissemination plan

D5.1. Generation of the Dissemination and Communication Plan

Action check Meeting 2

Agenda of the Action check meeting 2 and presentations

Recording

D3.1. Optimizing microscopy recording conditions

Alkyne-morpholino scale up

D2.3: Synthesise alkyne-tagged morpholinos, using newly developed methodology, on scales appropriate for the remainder of the project (up to 500 mg each)

Gene transcription detection

D34 Detection of single locus gene transcription by SRS in zebrafish embryos

SRS alkyne test

D1.5. SRS and Raman spectra of alkyne-tagged labels

Instrument support and maintenance

Report on instrument support and maintenance

Transcription hotspots

D33 Monitoring of transcription by alkyne labelled nucleotides in zebrafish

Websites, patent fillings, videos etc. (2)

Website

Creation of the project website

Outreach

Outreach activity plan

Open Research Data Pilot (1)

Open Research Data Pilot

Publications

Peer reviewed articles (19)

The Chemopreventive Effects of Polyphenols and Coffee, Based upon a DMBA Mouse Model with microRNA and mTOR Gene Expression Biomarkers

Author(s): Richard Molnar, Laszlo Szabo, Andras Tomesz, Arpad Deutsch, Richard Darago, Bence L. Raposa, Nowrasteh Ghodratollah, Timea Varjas, Balazs Nemeth, Zsuzsanna Orsos, Eva Pozsgai, Jozsef L. Szentpeteri, Ferenc Budan, and Istvan Kiss
Published in: Cells, 2022, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells11081300

Cell-type-specific inhibition of the dendritic plateau potential in striatal spiny projection neurons

Author(s): Kai Du, Yu-Wei Wu, Robert Lindroos, Yu Liu, Balázs Rózsa, Gergely Katona, Jun B. Ding, and Jeanette Hellgren Kotaleski
Published in: Proceedings of the National Academy of Sciences of the United States of America (PNAS), 2017, ISSN 1091-6490
Publisher: National Academy of Science
DOI: 10.1073/pnas.1704893114

In Vitro Determination of Inhibitory Effects of Humic Substances Complexing Zn and Se on SARS-CoV-2 Virus Replication

Author(s): Polett Hajdrik, Bernadett Pályi, Zoltán Kis,Noémi Kovács, Dániel Sándor Veres, Krisztián Szigeti, Ferenc Budán, Imre Hegedüs, Tibor Kovács, Ralf Bergmann and Domokos Máthé
Published in: Foods, 2022, ISSN 2304-8158
Publisher: MDPI
DOI: 10.3390/foods11050694

Transparent Thiol-ene/Acrylate-Based MicroECoG DevicesUsed for Concurrent Recording of Fluorescent CalciumSignals and Electrophysiology in Awake Animals

Author(s): Ágnes Szabó, Miklós Madarász, Zsófia Lantos, Anita Zátonyi, Vindhya Danda, Lisa Spurgin, Connie Manz, Balázs Rózsa, Zoltán Fekete
Published in: Advanced Materials Interfaces, 2022, ISSN 2196-7350
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/admi.202200729

Soft, Thiol-ene/Acrylate-Based Electrode Array for Long-Term Recording of Intracranial EEG Signals with ImprovedBiocompatibility in Mice

Author(s): Flóra Zsófia Fedor, Miklós Madarász, Anita Zátonyi, Ágnes Szabó, Tibor Lőrincz, Vindhya Danda, Lisa Spurgin, Connie Manz, Balázs Rózsa, Zoltán Fekete
Published in: Advanced Materials Technologies, 2021, ISSN 2365-709X
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/admt.202100942

Germ cell differentiation requires Tdrd7-dependent chromatin and transcriptome reprogramming marked by germ plasm relocalization

Author(s): D’Orazio, F., Balwierz, P., Jimenez-Gonzalez, A., Guo, Y., Hernández-Rodríguez, B., Wheatley, L., Hadzhev, Y., Jasiulewicz, A., Vaquerizas, JM., Cairns, B., Lenhard B. and Müller F.
Published in: Developmental cell, 2021, ISSN 1534-5807
Publisher: Cell Press
DOI: 10.1016/j.devcel.2021.02.007

Sharp-wave ripple doublets induce complex dendritic spikes in parvalbumin interneurons in vivo

Author(s): Linda Judák, Balázs Chiovini, Gábor Juhász, Dénes Pálfi, Zsolt Mezriczky, Zoltán Szadai, Gergely Katona, Benedek Szmola, Katalin Ócsai, Bernadett Martinecz, Anna Mihály, Ádám Dénes, Bálint Kerekes, Áron Szepesi, Gergely Szalay, István Ulbert, Zoltán Mucsi, Botond Roska & Balázs Rózsa
Published in: Nature Communications, 2022, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-34520-1

Difference in axon diameter and myelin thickness between excitatory and inhibitory callosally projecting axons in mice

Author(s): K.Basu, S.Appukuttan, R.Manchanda, A.Sík
Published in: Cerebral cortex, 2022, ISSN 1047-3211
Publisher: Oxford University Press
DOI: 10.1093/cercor/bhac329

Anticonvulsant activity of melatonin and its success in ameliorating epileptic comorbidity-like symptoms in zebrafish

Author(s): Q.Ren, D.Gao, L.Mou, S.Zhang, M.Zhang, N.Li, A.Sík, M.Jin, K.Liu
Published in: European journal of pharmacology, 2021, ISSN 0014-2999
Publisher: Elsevier BV
DOI: 10.1016/j.ejphar.2021.174589

Olive Oil Improves While Trans Fatty Acids Further Aggravate the Hypomethylation of LINE-1 Retrotransposon DNA in an Environmental Carcinogen Model

Author(s): L.Szabo, R.Molnar, A.Tomesz, A.Deutsch, R.Darago, T.Varjas, Z.Ritter, J.L.Szentpeteri, K.Andreidesz, D.Mathe, I.Hegedüs, A.Sík, F.Budan, I.Kiss
Published in: Nutrients, 2022, ISSN 2072-6643
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/nu14040908

Presynaptic NMDARs cooperate with local spikes toward GABA release from the reciprocal olfactory bulb granule cell spine

Author(s): Vanessa Lage-Rupprecht, Li Zhou, Gaia Bianchini, S Sara Aghvami, Max Mueller, Balázs Rózsa, Marco Sassoè-Pognetto, Veronica Egger
Published in: elife, 2020, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.63737

Changes in miR-124-1, miR-212, miR-132, miR-134, and miR-155 Expression Patterns after 7,12-Dimethylbenz(a)anthracene Treatment in CBA/Ca Mice

Author(s): Andras Tomesz, Laszlo Szabo, Richard Molnar, Arpad Deutsch, Richard Darago, Bence L. Raposa, Nowrasteh Ghodratollah, Timea Varjas, Balazs Nemeth, Zsuzsanna Orsos, Eva Pozsgai, Jozsef L. Szentpeteri, Ferenc Budan, and Istvan Kiss
Published in: Cells, 2022, ISSN 2073-4409
Publisher: MDPI
DOI: 10.3390/cells11061020

A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation

Author(s): Yavor Hadzhiev, Haseeb K. Qureshi, Lucy Wheatley, Ledean Cooper, Aleksandra Jasiulewicz, Huy Van Nguyen, Joseph W. Wragg, Divyasree Poovathumkadavil, Sascha Conic, Sarah Bajan, Attila Sik, György Hutvàgner, Làszlò Tora, Agnieszka Gambus, John S. Fossey, Ferenc Müller
Published in: Nature Communications, Issue 10/1, 2019, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-019-08487-5

Phosphino-Triazole Ligands for Palladium-Catalyzed Cross-Coupling

Author(s): Yiming Zhao, Huy van Nguyen, Louise Male, Philip Craven, Benjamin R. Buckley, John S. Fossey
Published in: Organometallics, Issue 37/22, 2018, Page(s) 4224-4241, ISSN 0276-7333
Publisher: American Chemical Society
DOI: 10.1021/acs.organomet.8b00539

α-asarone induces cardiac defects and QT prolongation through mitochondrial apoptosis pathway in zebrafish

Author(s): Xueliang Shang, Xiuna Ji, Jiao Dang, Lizhen Wang, Chen Sun, Kechun Liu, Attila Sik, Meng Jin
Published in: Toxicology Letters, Issue 324, 2020, Page(s) 1-11, ISSN 0378-4274
Publisher: Elsevier BV
DOI: 10.1016/j.toxlet.2020.02.003

Balancing Bulkiness in Gold(I) Phosphino-triazole Catalysis

Author(s): Yiming Zhao, Matthew G. Wakeling, Fernanda Meloni, Tze Jing Sum, Huy van Nguyen, Benjamin R. Buckley, Paul W. Davies, John S. Fossey
Published in: European Journal of Organic Chemistry, Issue 2019/31-32, 2019, Page(s) 5540-5548, ISSN 1434-193X
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/ejoc.201900850

Dendrite-Specific Amplification of Weak Synaptic Input during Network Activity In Vivo

Author(s): Leiron Ferrarese, Jean-Sébastien Jouhanneau, Michiel W.H. Remme, Jens Kremkow, Gergely Katona, Balázs Rózsa, Susanne Schreiber, James F.A. Poulet
Published in: Cell Reports, Issue 24/13, 2018, Page(s) 3455-3465.e5, ISSN 2211-1247
Publisher: Cell Press
DOI: 10.1016/j.celrep.2018.08.088

Possible involvement of TGF‑β‑SMAD‑mediated epithelial‑mesenchymal transition in pro‑metastatic property of PAX6

Author(s): Meng Jin, Daili Gao, Rongchun Wang, Attila Sik, Kechun Liu
Published in: Oncology Reports, Issue 44/2, 2020, Page(s) 555-564, ISSN 1021-335X
Publisher: Demetrios A. Spandidos Ed. & Pub.
DOI: 10.3892/or.2020.7644

Cortex-wide response mode of VIP-expressing inhibitory neurons by reward and punishment

Author(s): Zoltán Szadai, Hyun-Jae Pi, Quentin Chevy, Katalin Ócsai, Dinu F Albeanu, Balázs Chiovini, Gergely Szalay, Gergely Katona, Adam Kepecs
Published in: eLife, 2022, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.78815