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Nanoscale live-cell activity sensing using smart probes and imaging

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Publications

Structure–Function Dataset Reveals Environment Effects within a Fluorescent Protein Model System**

Author(s): Elke De Zitter, Siewert Hugelier, Sam Duwé, Wim Vandenberg, Alison G. Tebo, Luc Van Meervelt, Peter Dedecker
Published in: Angewandte Chemie International Edition, 60/18, 2021, Page(s) 10073-10081, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202015201

Design of experiments for the optimization of SOFI super-resolution microscopy imaging

Author(s): Dario Cevoli, Raffaele Vitale, Wim Vandenberg, Siewert Hugelier, Robin Van den Eynde, Peter Dedecker, Cyril Ruckebusch
Published in: Biomedical Optics Express, 12/5, 2021, Page(s) 2617, ISSN 2156-7085
Publisher: The Optical Society
DOI: 10.1364/boe.421168

Self-contained and modular structured illumination microscope

Author(s): Robin Van den Eynde, Wim Vandenberg, Siewert Hugelier, Arno Bouwens, Johan Hofkens, Marcel Müller, Peter Dedecker
Published in: Biomedical Optics Express, 12/7, 2021, Page(s) 4414, ISSN 2156-7085
Publisher: The Optical Society
DOI: 10.1364/boe.423492

Super-resolution imaging goes fast and deep

Author(s): Sam Duwé, Peter Dedecker
Published in: Nature Methods, 14/11, 2017, Page(s) 1042-1044, ISSN 1548-7091
Publisher: Nature Publishing Group
DOI: 10.1038/nmeth.4484

Correcting for photodestruction in super-resolution optical fluctuation imaging

Author(s): Yves Peeters, Wim Vandenberg, Sam Duwé, Arno Bouwens, Tomáš Lukeš, Cyril Ruckebusch, Theo Lasser, Peter Dedecker
Published in: Scientific Reports, 7/1, 2017, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-017-09666-4

Reduced Fluorescent Protein Switching Fatigue by Binding-Induced Emissive State Stabilization

Author(s): Thijs Roebroek, Sam Duwé, Wim Vandenberg, Peter Dedecker
Published in: International Journal of Molecular Sciences, 18/9, 2017, Page(s) 2015, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms18092015

Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

Author(s): S. Duwé, W. Vandenberg, P. Dedecker
Published in: Chemical Communications, 53/53, 2017, Page(s) 7242-7245, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/c7cc02344h

Efficient switching of mCherry fluorescence using chemical caging

Author(s): Bas M. C. Cloin, Elke De Zitter, Desiree Salas, Vincent Gielen, Gert E. Folkers, Marina Mikhaylova, Maike Bergeler, Bartosz Krajnik, Jeremy Harvey, Casper C. Hoogenraad, Luc Van Meervelt, Peter Dedecker, Lukas C. Kapitein
Published in: Proceedings of the National Academy of Sciences, 114/27, 2017, Page(s) 7013-7018, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1617280114

Effect of probe diffusion on the SOFI imaging accuracy

Author(s): Wim Vandenberg, Peter Dedecker
Published in: Scientific Reports, 7/1, 2017, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/srep44665

An extended quantitative model for super-resolution optical fluctuation imaging (SOFI)

Author(s): Wim Vandenberg, Marcel Leutenegger, Sam Duwé, Peter Dedecker
Published in: Optics Express, 27/18, 2019, Page(s) 25749, ISSN 1094-4087
Publisher: Optical Society of America
DOI: 10.1364/oe.27.025749

Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM

Author(s): Elke De Zitter, Daniel Thédié, Viola Mönkemöller, Siewert Hugelier, Joël Beaudouin, Virgile Adam, Martin Byrdin, Luc Van Meervelt, Peter Dedecker, Dominique Bourgeois
Published in: Nature Methods, 16/8, 2019, Page(s) 707-710, ISSN 1548-7091
Publisher: Nature Publishing Group
DOI: 10.1038/s41592-019-0462-3

Spatio-temporal correlation super-resolution optical fluctuation imaging

Author(s): A. Purohit, W. Vandenberg, T. Dertinger, D. Wöll, P. Dedecker, J. Enderlein
Published in: EPL (Europhysics Letters), 125/2, 2019, Page(s) 20005, ISSN 1286-4854
Publisher: IOP Publishing
DOI: 10.1209/0295-5075/125/20005

Quantitative comparison of camera technologies for cost-effective super-resolution optical fluctuation imaging (SOFI)

Author(s): Robin Van den Eynde, Alice Sandmeyer, Wim Vandenberg, Sam Duwé, Wolfgang Hübner, Thomas Huser, Peter Dedecker, Marcel Müller
Published in: Journal of Physics: Photonics, 1/4, 2019, Page(s) 044001, ISSN 2515-7647
Publisher: IOP Science
DOI: 10.1088/2515-7647/ab36ae

Optimizing the fluorescent protein toolbox and its use

Author(s): Sam Duwé, Peter Dedecker
Published in: Current Opinion in Biotechnology, 58, 2019, Page(s) 183-191, ISSN 0958-1669
Publisher: Elsevier BV
DOI: 10.1016/j.copbio.2019.04.006

Presenting a codon-optimized palette of fluorescent proteins for use in Candida albicans

Author(s): Wouter Van Genechten, Liesbeth Demuyser, Peter Dedecker, Patrick Van Dijck
Published in: Scientific Reports, 10/1, 2020, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-020-63308-w

SOFIevaluator: a strategy for the quantitative quality assessment of SOFI data

Author(s): Benjamien Moeyaert, Wim Vandenberg, Peter Dedecker
Published in: Biomedical Optics Express, 11/2, 2020, Page(s) 636, ISSN 2156-7085
Publisher: The Optical Society
DOI: 10.1364/boe.382278

Simultaneous readout of multiple FRET pairs using photochromism

Author(s): Thijs Roebroek, Wim Vandenberg, François Sipieter, Siewert Hugelier, Christophe Stove, Jin Zhang, Peter Dedecker
Published in: Nature Communications, 12/1, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-22043-0