Nanoscale live-cell activity sensing using smart probes and imaging

Project Information

NanoCellActivity

Grant agreement ID: 714688

DOI 10.3030/714688

Project closed

EC signature date 15 November 2016

Start date 1 February 2017

End date 31 July 2022

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 1 368 250,00

EU contribution € 1 368 250,00

Coordinated by

KATHOLIEKE UNIVERSITEIT LEUVEN
Belgium

Publications

Peer reviewed articles (26)

Structure–Function Dataset Reveals Environment Effects within a Fluorescent Protein Model System**

Author(s): Elke De Zitter, Siewert Hugelier, Sam Duwé, Wim Vandenberg, Alison G. Tebo, Luc Van Meervelt, Peter Dedecker
Published in: Angewandte Chemie International Edition, Issue 60/18, 2021, Page(s) 10073-10081, ISSN 1433-7851
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202015201

Design of experiments for the optimization of SOFI super-resolution microscopy imaging

Author(s): Dario Cevoli, Raffaele Vitale, Wim Vandenberg, Siewert Hugelier, Robin Van den Eynde, Peter Dedecker, Cyril Ruckebusch
Published in: Biomedical Optics Express, Issue 12/5, 2021, Page(s) 2617, ISSN 2156-7085
Publisher: The Optical Society
DOI: 10.1364/boe.421168

Fluorophore unmixing based on bleaching and recovery kinetics using MCR-ALS.

Author(s): Siewert Hugelier; R Van den Eynde; Wim Vandenberg; Wim Vandenberg; Peter Dedecker
Published in: Talanta, Issue 2, 2021, ISSN 1873-3573
Publisher: Elsevier
DOI: 10.1016/j.talanta.2021.122117

Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification

Author(s): Franziska Bierbuesse,Anaïs C Bourges,Vincent Gielen,Viola Mönkemöller,Wim Vandenberg,Yi Shen,Johan Hofkens,Pieter Vanden Berghe,Robert E Campbell,Benjamien Moeyaert,Peter Dedecker
Published in: Nature Communications, 2022, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-29508-w

Spectrally Tunable Förster Resonance Energy Transfer-Based Biosensors Using Organic Dye Grafting

Author(s): Marco Dalla Vecchia; Amandine Conte-Daban; Benjamin Cappe; Wim Vandenberg; Peter Vandenabeele; Franck B. Riquet; Peter Dedecker
Published in: ACS Sensors, Issue 3, 2022, ISSN 2379-3694
Publisher: ACS
DOI: 10.1021/acssensors.2c00066

Self-contained and modular structured illumination microscope

Author(s): Robin Van den Eynde, Wim Vandenberg, Siewert Hugelier, Arno Bouwens, Johan Hofkens, Marcel Müller, Peter Dedecker
Published in: Biomedical Optics Express, Issue 12/7, 2021, Page(s) 4414, ISSN 2156-7085
Publisher: The Optical Society
DOI: 10.1364/boe.423492

Orthogonal fluorescent chemogenetic reporters for multicolor imaging

Author(s): Alison G. Tebo; Benjamien Moeyaert; Marion Thauvin; Marion Thauvin; Irene Carlon-Andres; Dorothea Böken; Michel Volovitch; Michel Volovitch; Sergi Padilla-Parra; Sergi Padilla-Parra; Peter Dedecker; Sophie Vriz; Sophie Vriz; Arnaud Gautier; Arnaud Gautier
Published in: ISSN: 1552-4450, Issue 15524450, 2020, ISSN 1552-4450
Publisher: Nature Publishing Group
DOI: 10.1101/2020.04.04.022111

Model-free pixelation correction in SOFI imaging

Author(s): Franziska Bierbuesse; Vincent Gielen; Wim Vandenberg; Peter Dedecker
Published in: OSA Continuum, Issue 25787519, 2021, ISSN 2578-7519
Publisher: OSA
DOI: 10.1364/osac.413107

Super-resolution imaging goes fast and deep

Author(s): Sam Duwé, Peter Dedecker
Published in: Nature Methods, Issue 14/11, 2017, Page(s) 1042-1044, ISSN 1548-7091
Publisher: Nature Publishing Group
DOI: 10.1038/nmeth.4484

Correcting for photodestruction in super-resolution optical fluctuation imaging

Author(s): Yves Peeters, Wim Vandenberg, Sam Duwé, Arno Bouwens, Tomáš Lukeš, Cyril Ruckebusch, Theo Lasser, Peter Dedecker
Published in: Scientific Reports, Issue 7/1, 2017, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-017-09666-4

Reduced Fluorescent Protein Switching Fatigue by Binding-Induced Emissive State Stabilization

Author(s): Thijs Roebroek, Sam Duwé, Wim Vandenberg, Peter Dedecker
Published in: International Journal of Molecular Sciences, Issue 18/9, 2017, Page(s) 2015, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms18092015

Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

Author(s): S. Duwé, W. Vandenberg, P. Dedecker
Published in: Chemical Communications, Issue 53/53, 2017, Page(s) 7242-7245, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/c7cc02344h

Efficient switching of mCherry fluorescence using chemical caging

Author(s): Bas M. C. Cloin, Elke De Zitter, Desiree Salas, Vincent Gielen, Gert E. Folkers, Marina Mikhaylova, Maike Bergeler, Bartosz Krajnik, Jeremy Harvey, Casper C. Hoogenraad, Luc Van Meervelt, Peter Dedecker, Lukas C. Kapitein
Published in: Proceedings of the National Academy of Sciences, Issue 114/27, 2017, Page(s) 7013-7018, ISSN 0027-8424
Publisher: National Academy of Sciences
DOI: 10.1073/pnas.1617280114

Effect of probe diffusion on the SOFI imaging accuracy

Author(s): Wim Vandenberg, Peter Dedecker
Published in: Scientific Reports, Issue 7/1, 2017, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/srep44665

An extended quantitative model for super-resolution optical fluctuation imaging (SOFI)

Author(s): Wim Vandenberg, Marcel Leutenegger, Sam Duwé, Peter Dedecker
Published in: Optics Express, Issue 27/18, 2019, Page(s) 25749, ISSN 1094-4087
Publisher: Optical Society of America
DOI: 10.1364/oe.27.025749

Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM

Author(s): Elke De Zitter, Daniel Thédié, Viola Mönkemöller, Siewert Hugelier, Joël Beaudouin, Virgile Adam, Martin Byrdin, Luc Van Meervelt, Peter Dedecker, Dominique Bourgeois
Published in: Nature Methods, Issue 16/8, 2019, Page(s) 707-710, ISSN 1548-7091
Publisher: Nature Publishing Group
DOI: 10.1038/s41592-019-0462-3

Spatio-temporal correlation super-resolution optical fluctuation imaging

Author(s): A. Purohit, W. Vandenberg, T. Dertinger, D. Wöll, P. Dedecker, J. Enderlein
Published in: EPL (Europhysics Letters), Issue 125/2, 2019, Page(s) 20005, ISSN 1286-4854
Publisher: IOP Publishing
DOI: 10.1209/0295-5075/125/20005

Quantitative comparison of camera technologies for cost-effective super-resolution optical fluctuation imaging (SOFI)

Author(s): Robin Van den Eynde, Alice Sandmeyer, Wim Vandenberg, Sam Duwé, Wolfgang Hübner, Thomas Huser, Peter Dedecker, Marcel Müller
Published in: Journal of Physics: Photonics, Issue 1/4, 2019, Page(s) 044001, ISSN 2515-7647
Publisher: IOP Science
DOI: 10.1088/2515-7647/ab36ae

Optimizing the fluorescent protein toolbox and its use

Author(s): Sam Duwé, Peter Dedecker
Published in: Current Opinion in Biotechnology, Issue 58, 2019, Page(s) 183-191, ISSN 0958-1669
Publisher: Elsevier BV
DOI: 10.1016/j.copbio.2019.04.006

Presenting a codon-optimized palette of fluorescent proteins for use in Candida albicans

Author(s): Wouter Van Genechten, Liesbeth Demuyser, Peter Dedecker, Patrick Van Dijck
Published in: Scientific Reports, Issue 10/1, 2020, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-020-63308-w

SOFIevaluator: a strategy for the quantitative quality assessment of SOFI data

Author(s): Benjamien Moeyaert, Wim Vandenberg, Peter Dedecker
Published in: Biomedical Optics Express, Issue 11/2, 2020, Page(s) 636, ISSN 2156-7085
Publisher: The Optical Society
DOI: 10.1364/boe.382278

Quantitative Model for Reversibly Photoswitchable Sensors

Author(s): Adelizzi, Beatrice; Gielen, Vincent; Le Saux, Thomas; Dedecker, Peter; Jullien, Ludovic
Published in: "ACS Sensors, American Chemical Society, 2021, ⟨10.1021/acssensors.0c02414⟩", Issue 3, 2021, ISSN 2379-3694
Publisher: ACS

Smoothness correction for better SOFI imaging.

Author(s): Siewert Hugelier; Wim Vandenberg; Tomas Lukes; Kristin S. Grußmayer; Kristin S. Grußmayer; Paul H. C. Eilers; Peter Dedecker; Cyril Ruckebusch
Published in: Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021), Issue 11, 2021, Page(s) 1, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-021-87164-4

Quantitative Model for Reversibly Photoswitchable Sensors.

Author(s): Beatrice Adelizzi; Vincent Gielen; Thomas Le Saux; Peter Dedecker; Ludovic Jullien
Published in: ACS Sensors, Issue 23793694, 2021, ISSN 2379-3694
Publisher: American Chemical Society
DOI: 10.1021/acssensors.0c02414

Simultaneous readout of multiple FRET pairs using photochromism

Author(s): Thijs Roebroek, Wim Vandenberg, François Sipieter, Siewert Hugelier, Christophe Stove, Jin Zhang, Peter Dedecker
Published in: Nature Communications, Issue 12/1, 2021, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-021-22043-0

Separation of spectrally overlapping fluorophores using intra-exposure excitation modulation

Author(s): Hana Valenta; Siewert Hugelier; Sam Duwé; Giulia Lo Gerfo; Marcel Müller; Peter Dedecker; Wim Vandenberg
Published in: Biophysical Reports, Issue 26670747, 2021, ISSN 2667-0747
Publisher: Cell Press
DOI: 10.1016/j.bpr.2021.100026