Nanoscale live-cell activity sensing using smart probes and imaging

Publicaciones

Structure–Function Dataset Reveals Environment Effects within a Fluorescent Protein Model System**

Autores: Elke De Zitter, Siewert Hugelier, Sam Duwé, Wim Vandenberg, Alison G. Tebo, Luc Van Meervelt, Peter Dedecker
Publicado en: Angewandte Chemie International Edition, Edición 60/18, 2021, Página(s) 10073-10081, ISSN 1433-7851
Editor: John Wiley & Sons Ltd.
DOI: 10.1002/anie.202015201

Design of experiments for the optimization of SOFI super-resolution microscopy imaging

Autores: Dario Cevoli, Raffaele Vitale, Wim Vandenberg, Siewert Hugelier, Robin Van den Eynde, Peter Dedecker, Cyril Ruckebusch
Publicado en: Biomedical Optics Express, Edición 12/5, 2021, Página(s) 2617, ISSN 2156-7085
Editor: The Optical Society
DOI: 10.1364/boe.421168

Fluorophore unmixing based on bleaching and recovery kinetics using MCR-ALS.

Autores: Siewert Hugelier; R Van den Eynde; Wim Vandenberg; Wim Vandenberg; Peter Dedecker
Publicado en: Talanta, Edición 2, 2021, ISSN 1873-3573
Editor: Elsevier
DOI: 10.1016/j.talanta.2021.122117

Absolute measurement of cellular activities using photochromic single-fluorophore biosensors and intermittent quantification

Autores: Franziska Bierbuesse,Anaïs C Bourges,Vincent Gielen,Viola Mönkemöller,Wim Vandenberg,Yi Shen,Johan Hofkens,Pieter Vanden Berghe,Robert E Campbell,Benjamien Moeyaert,Peter Dedecker
Publicado en: Nature Communications, 2022, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-022-29508-w

Spectrally Tunable Förster Resonance Energy Transfer-Based Biosensors Using Organic Dye Grafting

Autores: Marco Dalla Vecchia; Amandine Conte-Daban; Benjamin Cappe; Wim Vandenberg; Peter Vandenabeele; Franck B. Riquet; Peter Dedecker
Publicado en: ACS Sensors, Edición 3, 2022, ISSN 2379-3694
Editor: ACS
DOI: 10.1021/acssensors.2c00066

Self-contained and modular structured illumination microscope

Autores: Robin Van den Eynde, Wim Vandenberg, Siewert Hugelier, Arno Bouwens, Johan Hofkens, Marcel Müller, Peter Dedecker
Publicado en: Biomedical Optics Express, Edición 12/7, 2021, Página(s) 4414, ISSN 2156-7085
Editor: The Optical Society
DOI: 10.1364/boe.423492

Orthogonal fluorescent chemogenetic reporters for multicolor imaging

Autores: Alison G. Tebo; Benjamien Moeyaert; Marion Thauvin; Marion Thauvin; Irene Carlon-Andres; Dorothea Böken; Michel Volovitch; Michel Volovitch; Sergi Padilla-Parra; Sergi Padilla-Parra; Peter Dedecker; Sophie Vriz; Sophie Vriz; Arnaud Gautier; Arnaud Gautier
Publicado en: ISSN: 1552-4450, Edición 15524450, 2020, ISSN 1552-4450
Editor: Nature Publishing Group
DOI: 10.1101/2020.04.04.022111

Model-free pixelation correction in SOFI imaging

Autores: Franziska Bierbuesse; Vincent Gielen; Wim Vandenberg; Peter Dedecker
Publicado en: OSA Continuum, Edición 25787519, 2021, ISSN 2578-7519
Editor: OSA
DOI: 10.1364/osac.413107

Super-resolution imaging goes fast and deep

Autores: Sam Duwé, Peter Dedecker
Publicado en: Nature Methods, Edición 14/11, 2017, Página(s) 1042-1044, ISSN 1548-7091
Editor: Nature Publishing Group
DOI: 10.1038/nmeth.4484

Correcting for photodestruction in super-resolution optical fluctuation imaging

Autores: Yves Peeters, Wim Vandenberg, Sam Duwé, Arno Bouwens, Tomáš Lukeš, Cyril Ruckebusch, Theo Lasser, Peter Dedecker
Publicado en: Scientific Reports, Edición 7/1, 2017, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-017-09666-4

Reduced Fluorescent Protein Switching Fatigue by Binding-Induced Emissive State Stabilization

Autores: Thijs Roebroek, Sam Duwé, Wim Vandenberg, Peter Dedecker
Publicado en: International Journal of Molecular Sciences, Edición 18/9, 2017, Página(s) 2015, ISSN 1422-0067
Editor: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms18092015

Live-cell monochromatic dual-label sub-diffraction microscopy by mt-pcSOFI

Autores: S. Duwé, W. Vandenberg, P. Dedecker
Publicado en: Chemical Communications, Edición 53/53, 2017, Página(s) 7242-7245, ISSN 1359-7345
Editor: Royal Society of Chemistry
DOI: 10.1039/c7cc02344h

Efficient switching of mCherry fluorescence using chemical caging

Autores: Bas M. C. Cloin, Elke De Zitter, Desiree Salas, Vincent Gielen, Gert E. Folkers, Marina Mikhaylova, Maike Bergeler, Bartosz Krajnik, Jeremy Harvey, Casper C. Hoogenraad, Luc Van Meervelt, Peter Dedecker, Lukas C. Kapitein
Publicado en: Proceedings of the National Academy of Sciences, Edición 114/27, 2017, Página(s) 7013-7018, ISSN 0027-8424
Editor: National Academy of Sciences
DOI: 10.1073/pnas.1617280114

Effect of probe diffusion on the SOFI imaging accuracy

Autores: Wim Vandenberg, Peter Dedecker
Publicado en: Scientific Reports, Edición 7/1, 2017, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/srep44665

An extended quantitative model for super-resolution optical fluctuation imaging (SOFI)

Autores: Wim Vandenberg, Marcel Leutenegger, Sam Duwé, Peter Dedecker
Publicado en: Optics Express, Edición 27/18, 2019, Página(s) 25749, ISSN 1094-4087
Editor: Optical Society of America
DOI: 10.1364/oe.27.025749

Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM

Autores: Elke De Zitter, Daniel Thédié, Viola Mönkemöller, Siewert Hugelier, Joël Beaudouin, Virgile Adam, Martin Byrdin, Luc Van Meervelt, Peter Dedecker, Dominique Bourgeois
Publicado en: Nature Methods, Edición 16/8, 2019, Página(s) 707-710, ISSN 1548-7091
Editor: Nature Publishing Group
DOI: 10.1038/s41592-019-0462-3

Spatio-temporal correlation super-resolution optical fluctuation imaging

Autores: A. Purohit, W. Vandenberg, T. Dertinger, D. Wöll, P. Dedecker, J. Enderlein
Publicado en: EPL (Europhysics Letters), Edición 125/2, 2019, Página(s) 20005, ISSN 1286-4854
Editor: IOP Publishing
DOI: 10.1209/0295-5075/125/20005

Quantitative comparison of camera technologies for cost-effective super-resolution optical fluctuation imaging (SOFI)

Autores: Robin Van den Eynde, Alice Sandmeyer, Wim Vandenberg, Sam Duwé, Wolfgang Hübner, Thomas Huser, Peter Dedecker, Marcel Müller
Publicado en: Journal of Physics: Photonics, Edición 1/4, 2019, Página(s) 044001, ISSN 2515-7647
Editor: IOP Science
DOI: 10.1088/2515-7647/ab36ae

Optimizing the fluorescent protein toolbox and its use

Autores: Sam Duwé, Peter Dedecker
Publicado en: Current Opinion in Biotechnology, Edición 58, 2019, Página(s) 183-191, ISSN 0958-1669
Editor: Elsevier BV
DOI: 10.1016/j.copbio.2019.04.006

Presenting a codon-optimized palette of fluorescent proteins for use in Candida albicans

Autores: Wouter Van Genechten, Liesbeth Demuyser, Peter Dedecker, Patrick Van Dijck
Publicado en: Scientific Reports, Edición 10/1, 2020, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-020-63308-w

SOFIevaluator: a strategy for the quantitative quality assessment of SOFI data

Autores: Benjamien Moeyaert, Wim Vandenberg, Peter Dedecker
Publicado en: Biomedical Optics Express, Edición 11/2, 2020, Página(s) 636, ISSN 2156-7085
Editor: The Optical Society
DOI: 10.1364/boe.382278

Quantitative Model for Reversibly Photoswitchable Sensors

Autores: Adelizzi, Beatrice; Gielen, Vincent; Le Saux, Thomas; Dedecker, Peter; Jullien, Ludovic
Publicado en: "ACS Sensors, American Chemical Society, 2021, ⟨10.1021/acssensors.0c02414⟩", Edición 3, 2021, ISSN 2379-3694
Editor: ACS

Smoothness correction for better SOFI imaging.

Autores: Siewert Hugelier; Wim Vandenberg; Tomas Lukes; Kristin S. Grußmayer; Kristin S. Grußmayer; Paul H. C. Eilers; Peter Dedecker; Cyril Ruckebusch
Publicado en: Scientific Reports, Vol 11, Iss 1, Pp 1-9 (2021), Edición 11, 2021, Página(s) 1, ISSN 2045-2322
Editor: Nature Publishing Group
DOI: 10.1038/s41598-021-87164-4

Quantitative Model for Reversibly Photoswitchable Sensors.

Autores: Beatrice Adelizzi; Vincent Gielen; Thomas Le Saux; Peter Dedecker; Ludovic Jullien
Publicado en: ACS Sensors, Edición 23793694, 2021, ISSN 2379-3694
Editor: American Chemical Society
DOI: 10.1021/acssensors.0c02414

Simultaneous readout of multiple FRET pairs using photochromism

Autores: Thijs Roebroek, Wim Vandenberg, François Sipieter, Siewert Hugelier, Christophe Stove, Jin Zhang, Peter Dedecker
Publicado en: Nature Communications, Edición 12/1, 2021, ISSN 2041-1723
Editor: Nature Publishing Group
DOI: 10.1038/s41467-021-22043-0

Separation of spectrally overlapping fluorophores using intra-exposure excitation modulation

Autores: Hana Valenta; Siewert Hugelier; Sam Duwé; Giulia Lo Gerfo; Marcel Müller; Peter Dedecker; Wim Vandenberg
Publicado en: Biophysical Reports, Edición 26670747, 2021, ISSN 2667-0747
Editor: Cell Press
DOI: 10.1016/j.bpr.2021.100026

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