Molecular Tools for Nucleic Acid Manipulation for Biological Intervention

Project Information

MMBio

Grant agreement ID: 721613

DOI 10.3030/721613

Project closed

EC signature date 31 August 2016

Start date 1 January 2017

End date 31 August 2021

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost € 3 971 347,92

EU contribution € 3 971 347,92

Coordinated by

THE CHANCELLOR MASTERS AND SCHOLARS OF THE UNIVERSITY OF CAMBRIDGE
United Kingdom

Deliverables

Documents, reports (1)

Public engagement and dissemination of achievements

month 44: Participation in public open day to disseminate the project outcomes

Other (3)

Summer School

A five day summer school, open to the public, will be organised by beneficiary KI entitled Nucleic Acids in Therapy

Science communication course

(month 8): a Science Communication course that develops their skills in conveying complex scientific ideas to a general audience, and provide the technical skills to prepare videos and podcasts will be provided.

Network Conference

month 44 A twoday conference to showcase the research area and the achievements of the network accessible to the general public This can be a free standing event or part of a relevant conference eg the Annual Meetings of the Oligonucleotide Therapeutics Society or the International Round Table on Nucleosides Nucleotides and Nucleic Acids in Stockholm JuneJuly 2018

Websites, patent fillings, videos etc. (1)

Website, ESR webpages and Moodle operational

Publications

Peer reviewed articles (22)

Lipophilic Peptide Dendrimers for Delivery of Splice-Switching Oligonucleotides

Author(s): Haneen Daralnakhla, Osama Saher, Susanna Zamolo, Safa Bazaz, Jeremy P. Bost, Marc Heitz, Karin E. Lundin, Samir EL Andaloussi, Tamis Darbre, Jean-Louis Reymond, Rula Zain, C. I. Edvard Smith
Published in: Pharmaceutics, Issue 13/1, 2021, Page(s) 116, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics13010116

Sequence dependence of Pd(II)-mediated base pairing by palladacyclic nucleobase surrogates

Author(s): Madhuri Hande, Sajal Maity, Tuomas Lönnberg
Published in: Journal of Inorganic Biochemistry, Issue 222, 2021, Page(s) 111506, ISSN 0162-0134
Publisher: Elsevier BV
DOI: 10.1016/j.jinorgbio.2021.111506

Oligonucleotides Targeting DNA Repeats Downregulate Huntingtin Gene Expression in Huntington's Patient-Derived Neural Model System

Author(s): Tea Umek, Thomas Olsson, Olof Gissberg, Osama Saher, Eman M. Zaghloul, Karin E. Lundin, Jesper Wengel, Eric Hanse, Henrik Zetterberg, Dzeneta Vizlin-Hodzic, C. I. Edvard Smith, Rula Zain
Published in: Nucleic Acid Therapeutics, 2021, ISSN 2159-3337
Publisher: Mary Ann Liebert Inc.
DOI: 10.1089/nat.2021.0021

PNA-Based MicroRNA Detection Methodologies

Author(s): Enrico Cadoni, Alex Manicardi, Annemieke Madder
Published in: Molecules, Issue 25/6, 2020, Page(s) 1296, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules25061296

Beyond small molecules: targeting G-quadruplex structures with oligonucleotides and their analogues

Author(s): Enrico Cadoni, Lessandro De Paepe, Alex Manicardi, Annemieke Madder
Published in: Nucleic Acids Research, Issue 49/12, 2021, Page(s) 6638-6659, ISSN 0305-1048
Publisher: Oxford University Press
DOI: 10.1093/nar/gkab334

Metal‐Mediated Base Pairing of Rigid and Flexible Benzaldoxime Metallacycles

Author(s): Sajal Maity, Madhuri Hande, Tuomas Lönnberg
Published in: ChemBioChem, Issue 21/16, 2020, Page(s) 2321-2328, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.202000135

Transfecting tissue models with CRISPR/Cas9 plasmid DNA using peptide dendrimers

Author(s): Susanna J. Zamolo, Tamis Darbre, Jean-Louis Reymond
Published in: Chemical Communications, Issue 56/80, 2020, Page(s) 11981-11984, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0cc04750c

Further Probing of Cu2+-Dependent PNAzymes Acting as Artificial RNA Restriction Enzymes.

Author(s): Olivia Luige; Merita Murtola; Alice Ghidini; Roger Strömberg
Published in: Molecules, 24 (4), Issue 1, 2019, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3929/ethz-b-000327274

The Mechanism of Cleavage of RNA Phosphodiesters by a Gold Nanoparticle Nanozyme

Author(s): Joanna Czescik, Fabrizio Mancin, Roger Strömberg, Paolo Scrimin
Published in: Chemistry – A European Journal, Issue 27/31, 2021, Page(s) 8143-8148, ISSN 0947-6539
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/chem.202100299

Teaching photosensitizers a new trick: red light-triggered G-quadruplex alkylation by ligand co-localization

Author(s): Enrico Cadoni, Alex Manicardi, Mathieu Fossépré, Kaat Heirwegh, Mathieu Surin, Annemieke Madder
Published in: Chemical Communications, Issue 57/8, 2021, Page(s) 1010-1013, ISSN 1359-7345
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0cc06030e

Synthesis and Hybridization Properties of Covalently Mercurated and Palladated Oligonucleotides

Author(s): Dattatraya Ukale, Sajal Maity, Madhuri Hande, Tuomas Lönnberg
Published in: Synlett, Issue 30/15, 2019, Page(s) 1733-1737, ISSN 0936-5214
Publisher: Georg Thieme Verlag
DOI: 10.1055/s-0037-1611821

Palladacyclic Conjugate Group Promotes Hybridization of Short Oligonucleotides

Author(s): Madhuri Hande, Sajal Maity, Tuomas Lönnberg
Published in: International Journal of Molecular Sciences, Issue 19/6, 2018, Page(s) 1588, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms19061588

A Versatile and Convenient Synthesis of 34 S-Labeled Phosphorothioate Oligonucleotides

Author(s): Rouven Stulz, Johan Meuller, Dženita Baždarević, Charlotte Wennberg Huldt, Roger Strömberg, Shalini Andersson, Anders Dahlén
Published in: ChemBioChem, Issue 19/19, 2018, Page(s) 2114-2119, ISSN 1439-4227
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cbic.201800417

Oligonucleotide–Palladacycle Conjugates as Splice-Correcting Agents

Author(s): Madhuri Hande, Osama Saher, Karin Lundin, C. Smith, Rula Zain, Tuomas Lönnberg
Published in: Molecules, Issue 24/6, 2019, Page(s) 1180, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules24061180

Exploiting Double Exchange Diels-Alder Cycloadditions for Immobilization of Peptide Nucleic Acids on Gold Nanoparticles

Author(s): Enrico Cadoni, Daniele Rosa-Gastaldo, Alex Manicardi, Fabrizio Mancin, Annemieke Madder
Published in: Frontiers in Chemistry, Issue 8, 2020, ISSN 2296-2646
Publisher: Frontiers
DOI: 10.3389/fchem.2020.00004

Factors Influencing the Activity of Nanozymes in the Cleavage of an RNA Model Substrate

Author(s): Czescik, Zamolo, Darbre, Mancin, Scrimin
Published in: Molecules, Issue 24/15, 2019, Page(s) 2814, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules24152814

Multifunctional, CD44v6-Targeted ORMOSIL Nanoparticles Enhance Drugs Toxicity in Cancer Cells

Author(s): Morillas-Becerril, Peta, Gabrielli, Russo, Lubian, Nodari, Ferlin, Scrimin, Palù, Barzon, Castagliuolo, Mancin, Trevisan
Published in: Nanomaterials, Issue 10/2, 2020, Page(s) 298, ISSN 2079-4991
Publisher: MDPI
DOI: 10.3390/nano10020298

Oligonucleotide Binding to Non-B-DNA in MYC

Author(s): Tea Umek, Karin Sollander, Helen Bergquist, Jesper Wengel, Karin E. Lundin, C.I. Edvard Smith, Rula Zain
Published in: Molecules, Issue 24/5, 2019, Page(s) 1000, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules24051000

Sugar and Polymer Excipients Enhance Uptake and Splice-Switching Activity of Peptide-Dendrimer/Lipid/Oligonucleotide Formulations

Author(s): Osama Saher, Taavi Lehto, Olof Gissberg, Dhanu Gupta, Oskar Gustafsson, Samir EL Andaloussi, Tamis Darbre, Karin E. Lundin, C. I. Edvard Smith, Rula Zain
Published in: Pharmaceutics, Issue 11/12, 2019, Page(s) 666, ISSN 1999-4923
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/pharmaceutics11120666

Poly(lipoic acid)-Based Nanoparticles as Self-Organized, Biocompatible, and Corona-Free Nanovectors

Author(s): Jakub W. Trzciński, Lucía Morillas-Becerril, Sara Scarpa, Marco Tannorella, Francesco Muraca, Federico Rastrelli, Chiara Castellani, Marny Fedrigo, Annalisa Angelini, Regina Tavano, Emanuele Papini, Fabrizio Mancin
Published in: Biomacromolecules, Issue 22/2, 2021, Page(s) 467-480, ISSN 1525-7797
Publisher: American Chemical Society
DOI: 10.1021/acs.biomac.0c01321

Visible-light triggered templated ligation on surface using furan-modified PNAs

Author(s): Alex Manicardi, Enrico Cadoni, Annemieke Madder
Published in: Chemical Science, Issue 11/43, 2020, Page(s) 11729-11739, ISSN 2041-6520
Publisher: Royal Society of Chemistry
DOI: 10.1039/d0sc04875e

New Alkyne and Amine Linkers for Versatile Multiple Conjugation of Oligonucleotides

Author(s): Dmytro Honcharenko, Kristina Druceikaite, Malgorzata Honcharenko, Martin Bollmark, Ulf Tedebark, Roger Strömberg
Published in: ACS Omega, Issue 6/1, 2021, Page(s) 579-593, ISSN 2470-1343
Publisher: American Chemical Society
DOI: 10.1021/acsomega.0c05075