Project description
In vivo light sheet microscopy in zebrafish embryos sheds light on cardiogenesis
From 1990 to 2021, deaths around the world from cardiovascular disease jumped from around 12 million people to around 20 million and more children with congenital heart disease, the most prevalent of birth defects, are surviving. With the support of the Marie Skłodowska-Curie Actions programme, the 4DHeart project will investigate the genetic and environmental factors influencing cardiovascular development using the well-known zebrafish model to visualise cardiac development in vivo. The training programme will harness revolutionary light sheet microscopy and introduce tools and techniques including advanced software and optical tweezers. With these, the team will create a toolbox compatible with existing software packages allowing holistic modeling of zebrafish cardiac morphogenesis.
Objective
Cardiovascular (CV) disease is a main cause of death worldwide. During adulthood, ischemic heart disease leads to heart failure and perinatally, congenital heart defects are found in over 20% of deaths. Moreover, genetic or epigenetic factors altering development can have an impact much later in life. These facts underscore the need of a better understanding of the genetic and environmental factors that influence CV development. An important way to increase our knowledge is by visualizing cardiac development in vivo. Recent advance in microscopy allows monitoring CV development at a cellular level in organisms such as the zebrafish model. Particularly revolutionary has been the development of light sheet microscopy (LSM). We want to further exploit LSM for in vivo manipulation of cells in the embryonic zebrafish heart and measure with high precision biophysical parameters, by introducing novel features to LSM such as optical tweezers. High throughput cardiac imaging protocols for zebrafish larvae suitable for screenings will be set up. We will develop softwares to enhance resolution of acquisition, large dataset handling and image-processing. The aim is to generate a toolbox to be implemented into existing software packages allowing a complete modeling of zebrafish cardiac morphogenesis. We will adapt LSM for adult zebrafish hearts to study cardiac regeneration and mouse heart development at cellular resolution. Each Early Stage Researchers (ESRs) will develop their own technology to solve a biological problem at the frontier of knowledge. ESRs will receive multidisciplinary (CV development, physics, biocomputing, bioimaging) as well as intersectorial (academic research, SMEs, large companies) training and will achieve unique skills on microscopy, in-vivo imaging and image analysis allowing them to interrogate questions on cardiac development and regeneration. Their profile will be at the interface of a bioengineer and a life science researcher filling a currently existing gap on the market.
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Coordinator
28029 Madrid
Spain