High-valent protein-coordinated catalytic metal sites: Geometric and Electronic ARchitecture

Project Information

HIGH-GEAR

Grant agreement ID: 724394

DOI 10.3030/724394

Project closed

EC signature date 30 January 2017

Start date 1 June 2017

End date 31 May 2023

Funded under

• EXCELLENT SCIENCE - European Research Council (ERC)

Total cost € 1 968 375,00

EU contribution € 1 968 375,00

Coordinated by

STOCKHOLMS UNIVERSITET
Sweden

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Publications

Peer reviewed articles (14)

Key Structural Motifs Balance Metal Binding and Oxidative Reactivity in a Heterobimetallic Mn/Fe Protein.

Author(s): Effie C. Kisgeropoulos, Julia J. Griese, Zachary R. Smith, Rui M. M. Branca, Camille R. Schneider, Martin Högbom, Hannah S. Shafaat
Published in: Journal of the American Chemical Society, Issue 142/11, 2020, Page(s) 5338–5354, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.0c00333

High-Resolution XFEL Structure of the Soluble Methane Monooxygenase Hydroxylase Complex with its Regulatory Component at Ambient Temperature in Two Oxidation States.

Author(s): Vivek Srinivas, Rahul Banerjee, Hugo Lebrette, Jason C. Jones, Oskar Aurelius, In-Sik Kim, Cindy C. Pham, Sheraz Gul, K.D. Sutherlin, Asmit Bhowmick, Juliane John, Esra Bozkurt, Thomas Fransson, Pierre Aller, Agata Butryn, Isabel Bogacz, Philipp Stefan Simon, Stephen Keable, Alexander Britz, Kensuke Tono, Kyung Sook Kim, Sang-Youn Park, Sang-Jae Lee, Jaehyun Park, Roberto Alonso-Mori, Franklin D.
Published in: Journal of the American Chemical Society, Issue 142/33, 2020, Page(s) 14249–14266, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.0c05613

Redox-controlled reorganization and flavin strain within the ribonucleotide reductase R2b-NrdI complex monitored by serial femtosecond crystallography

Author(s): John J, Aurelius O, Srinivas V, Saura P, Kim IS, Bhowmick A, Simon PS, Dasgupta M, Pham C, Gul S, Sutherlin KD, Aller P, Butryn A, Orville AM, Cheah MH, Owada S, Tono K, Fuller FD, Batyuk A, Brewster AS, Sauter NK, Yachandra VK, Yano J, Kaila VRI, Kern J, Lebrette H, Högbom M
Published in: eLife, Issue 2022 Sep 9;11, 2022, Page(s) e79226, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.79226

Ether cross-link formation in the R2-like ligand-binding oxidase

Author(s): Julia J. Griese, Rui M. M. Branca, Vivek Srinivas, Martin Högbom
Published in: JBIC Journal of Biological Inorganic Chemistry, Issue 23/6, 2018, Page(s) 879-886, ISSN 0949-8257
Publisher: Springer Verlag
DOI: 10.1007/s00775-018-1583-3

Metal-free ribonucleotide reduction powered by a DOPA radical in Mycoplasma pathogens

Author(s): Vivek Srinivas, Hugo Lebrette, Daniel Lundin, Yuri Kutin, Margareta Sahlin, Michael Lerche, Jürgen Eirich, Rui M. M. Branca, Nicholas Cox, Britt-Marie Sjöberg, Martin Högbom
Published in: Nature, Issue 563/7731, 2018, Page(s) 416-420, ISSN 0028-0836
Publisher: Nature Publishing Group
DOI: 10.1038/s41586-018-0653-6

Driving Protein Conformational Changes with Light: Photoinduced Structural Rearrangement in a Heterobimetallic Oxidase

Author(s): Pearson T. Maugeri, Julia J. Griese, Rui M. Branca, Effie K. Miller, Zachary R. Smith, Jürgen Eirich, Martin Högbom, Hannah S. Shafaat
Published in: Journal of the American Chemical Society, Issue 140/4, 2018, Page(s) 1471-1480, ISSN 0002-7863
Publisher: American Chemical Society
DOI: 10.1021/jacs.7b11966

Chemical flexibility of heterobimetallic Mn/Fe cofactors: R2lox and R2c proteins

Author(s): Yury Kutin, Ramona Kositzki, Rui M. M. Branca, Vivek Srinivas, Daniel Lundin, Michael Haumann, Martin Högbom, Nicholas Cox, Julia J. Griese
Published in: Journal of Biological Chemistry, Issue 294/48, 2019, Page(s) 18372-18386, ISSN 0021-9258
Publisher: American Society for Biochemistry and Molecular Biology Inc.
DOI: 10.1074/jbc.ra119.010570

Assembly of a heterodinuclear Mn/Fe cofactor is coupled to tyrosine–valine ether cross-link formation in the R2-like ligand-binding oxidase

Author(s): Julia J. Griese, Ramona Kositzki, Michael Haumann, Martin Högbom
Published in: JBIC Journal of Biological Inorganic Chemistry, Issue 24/2, 2019, Page(s) 211-221, ISSN 0949-8257
Publisher: Springer Verlag
DOI: 10.1007/s00775-019-01639-4

Location-specific quantification of protein-bound metal ions by X-ray anomalous dispersion: Q-XAD

Author(s): Julia J. Griese, Martin Högbom
Published in: Acta Crystallographica Section D Structural Biology, Issue 75/8, 2019, Page(s) 764-771, ISSN 2059-7983
Publisher: International Union of Crystallography
DOI: 10.1107/s2059798319009926

Solving a new R2lox protein structure by microcrystal electron diffraction

Author(s): Hongyi Xu, Hugo Lebrette, Max T. B. Clabbers, Jingjing Zhao, Julia J. Griese, Xiaodong Zou, Martin Högbom
Published in: Science Advances, Issue 5/8, 2019, Page(s) eaax4621, ISSN 2375-2548
Publisher: AAAS
DOI: 10.1126/sciadv.aax4621

Redox-induced structural changes in the di-iron and di-manganese forms of Bacillus anthracis ribonucleotide reductase subunit NrdF suggest a mechanism for gating of radical access

Author(s): Kristīne Grāve, Wietske Lambert, Gustav Berggren, Julia J. Griese, Matthew D. Bennett, Derek T. Logan, Martin Högbom
Published in: JBIC Journal of Biological Inorganic Chemistry, Issue 24/6, 2019, Page(s) 849-861, ISSN 0949-8257
Publisher: Springer Verlag
DOI: 10.1007/s00775-019-01703-z

Fate of oxygen species from O2 activation at dimetal cofactors in an oxidase enzyme revealed by 57Fe nuclear resonance X-ray scattering and quantum chemistry

Author(s): Stefan Mebs, Vivek Srinivas, Ramona Kositzki, Julia J. Griese, Martin Högbom, Michael Haumann
Published in: Biochimica et Biophysica Acta (BBA) - Bioenergetics, Issue 1860/12, 2019, Page(s) 148060, ISSN 0005-2728
Publisher: Elsevier BV
DOI: 10.1016/j.bbabio.2019.148060

The Bacillus anthracis class Ib ribonucleotide reductase subunit NrdF intrinsically selects manganese over iron

Author(s): Kristīne Grāve, Julia J. Griese, Gustav Berggren, Matthew D. Bennett, Martin Högbom
Published in: JBIC Journal of Biological Inorganic Chemistry, Issue 25/4, 2020, Page(s) 571-582, ISSN 0949-8257
Publisher: Springer Verlag
DOI: 10.1007/s00775-020-01782-3

Comparative structural analysis provides new insights into the function of R2-like ligand-binding oxidase

Author(s): Martin Högbom; Hugo Lebrette; Annika Johansson; Riccardo Diamanti; Julia J. Griese; Vivek Srinivas; Anders Nordström
Published in: FEBS Letters, 2022, ISSN 0014-5793
Publisher: Elsevier BV
DOI: 10.1002/1873-3468.14319