Objetivo
The purpose of this research is the experimental toxicological study of the chemical and pulmonary biological reactivity of dust deposits consisting essentially of ferrous and ferric compounds, whether or not in the presence of polycyclic aromatic hydrocarbons.
Evaluation of dose-effect relationships.
Study of the dose-effect relationships specific to iron particles, by comparison with basic (CaO) and acidic (SiO2) particles, whether or not doped.
Study of the biochemical transformation of iron oxide-based particles, whether or not in the presence of PAHs:
* the metabolization of iron and PAHs;
* structural changes in the particles.
Assessment of the biological effects on lung tissue cell cultures of exposure to such particles:
* morphological and functional changes in the lung tissue cells;
* formation of free radicals;
* lipid peroxidation.
Study of the mechanisms of mutagenesis.
METHODS AND MEANS BY WHICH THE AIMS ARE TO BE ACHIEVED
. Manufacture and characterization of particles (LSMCL)
. Preliminary studies on the behaviour and stability of ferrous iron (with or without PAHs) in culture environments (with foetal calf serum)
. Toxicological studies
. Genotoxicity study (Prof. Marzin)
. In vitro study
. In vivo tests
. Molecular modelling (IMTNF)
Tema(s)
Data not availableConvocatoria de propuestas
Data not availableRégimen de financiación
CSC - Cost-sharing contractsCoordinador
57120 ROMBAS
Francia