Multisystem Cell Therapy for Improvement of Urinary Continence

Project Information

MUSIC

Grant agreement ID: 731377

DOI 10.3030/731377

Project closed

EC signature date 29 November 2016

Start date 1 January 2017

End date 31 March 2022

Funded under

• SOCIETAL CHALLENGES - Health, demographic change and well-being

Total cost € 6 623 628,75

EU contribution € 2 349 521,25

Coordinated by

UNIVERSITAT ZURICH
Switzerland

Deliverables

Demonstrators, pilots, prototypes (2)

Cell jet injection established and evaluated in vitro and in vivo

Water jet cell injection technique to apply viable MPCs in vitro on surfaces as well as in predetermined areas of the urethral sphincter muscle explants.

Definition of the best option for delivery of MPCs

Conclusion if needle-less injection of MPCs is superior to needle-application and recommendation for further surgical regimen and cell application strategy.

Documents, reports (1)

CE Certification of Injection Device

The injection of the final MPC product will be performed during Treatment Visit under general or spinal anesthesia. To allow standardized injections into the pelvic floor of female patients, the cells will be injected under real-time ultrasound guidance by the use of an Injection device (linker-device between ultrasound and syringe with needle) As required by Swissmedic (Swiss Regulatory Agency), the developed Injection Device will be CE certified before start of the clinical trial by the Swiss Center of Regeneration Medicine (SCRM) of UZH.

Websites, patent fillings, videos etc. (2)

Project webpage online

The MUSIC website will combine functionalities to streamline communication and material exchange between partners, as well as to monitor progress and dissemination of project results to stakeholders. A password-protected internal part of the website will be accessible for all project Participants only, providing helpful information, documents and schedules. To raise the public interest, this website will disseminate any information which can be disclosed to the public and it will be updated regularly.

Workshops to be held within the consortium and external candidates

XPD and UZH will organize yearly workshops for translational stem cell research for the other consortium partners as well as for external candidates from universities hospitals and industry

Publications

Peer reviewed articles (17)

Evaluation of Urinary Sphincter Function by Rapid Magnetic Resonance Diffusion Tensor Imaging

Author(s): Florian A. Schmid, Ryszard S. Gomolka, Andreas M. Hötker, Andreas Boss, Thomas M. Kessler, Cristina Rossi, Daniel Eberli
Published in: International Neurourology Journal, Issue 24/4, 2020, Page(s) 349-357, ISSN 2093-6931
Publisher: International Neurourology Journal
DOI: 10.5213/inj.2040208.104

Moderne Inkontinenztherapie mittels Muskelstammzellen

Author(s): Florian Schmid, Daniel Eberli
Published in: Praxis, Issue 109/6, 2020, Page(s) 447-452, ISSN 1661-8157
Publisher: Verlag Hans Huber
DOI: 10.1024/1661-8157/a003421

Feasibility, technique and accuracy of ultrasound-guided transurethral injections into the urinary sphincter of female cadavers: proof of concept

Author(s): Florian A. Schmid, Dominic Gascho, Niklaus Zoelch, Jenny A. Prange, Giovanni Colacicco, Daniel Eberli
Published in: BMC Urology, Issue 20/1, 2020, ISSN 1471-2490
Publisher: BioMed Central
DOI: 10.1186/s12894-020-00719-x

Hypoxic Conditions Promote the Angiogenic Potential of Human Induced Pluripotent Stem Cell-Derived Extracellular Vesicles

Author(s): André Cronemberger Andrade, Martin Wolf, Heide-Marie Binder, Fausto Gueths Gomes, Felix Manstein, Patricia Ebner-Peking, Rodolphe Poupardin, Robert Zweigerdt, Katharina Schallmoser, Dirk Strunk
Published in: International Journal of Molecular Sciences, Issue 22/8, 2021, Page(s) 3890, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22083890

Novel Techniques to Improve Precise Cell Injection

Author(s): Walter Linzenbold, Andreas Fech, Manuela Hofmann, Wilhelm K. Aicher, Markus D. Enderle
Published in: International Journal of Molecular Sciences, Issue 22/12, 2021, Page(s) 6367, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22126367

Acoustophoresis Enables the Label‐Free Separation of Functionally Different Subsets of Cultured Bone Marrow Stromal Cells

Author(s): Franziska Olm, Hooi Ching Lim, Katharina Schallmoser, Dirk Strunk, Thomas Laurell, Stefan Scheding
Published in: Cytometry Part A, Issue 99/5, 2021, Page(s) 476-487, ISSN 1552-4922
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/cyto.a.24171

Human Platelet Lysate for Good Manufacturing Practice-Compliant Cell Production

Author(s): Michaela Oeller, Sandra Laner-Plamberger, Linda Krisch, Eva Rohde, Dirk Strunk, Katharina Schallmoser
Published in: International Journal of Molecular Sciences, Issue 22/10, 2021, Page(s) 5178, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22105178

Rapid and precise delivery of cells in the urethral sphincter complex by a novel needle‐free waterjet technology

Author(s): Walter Linzenbold, Luise Jäger, Hartmut Stoll, Tanja Abruzzese, Niklas Harland, Nicolas Bézière, Andreas Fech, Markus Enderle, Bastian Amend, Arnulf Stenzl, Wilhelm K. Aicher
Published in: BJU International, Issue 127/4, 2021, Page(s) 463-472, ISSN 1464-4096
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/bju.15249

Large Animal Models for Investigating Cell Therapies of Stress Urinary Incontinence

Author(s): Bastian Amend, Niklas Harland, Jasmin Knoll, Arnulf Stenzl, Wilhelm K. Aicher
Published in: International Journal of Molecular Sciences, Issue 22/11, 2021, Page(s) 6092, ISSN 1422-0067
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/ijms22116092

Selection of Tissue Factor-Deficient Cell Transplants as a Novel Strategy for Improving Hemocompatibility of Human Bone Marrow Stromal Cells

Author(s): Michaela Oeller, Sandra Laner-Plamberger, Sarah Hochmann, Nina Ketterl, Martina Feichtner, Gabriele Brachtl, Anna Hochreiter, Cornelia Scharler, Lara Bieler, Pasquale Romanelli, Sebastien Couillard-Despres, Elisabeth Russe, Katharina Schallmoser, Dirk Strunk
Published in: Theranostics, Issue 8/5, 2018, Page(s) 1421-1434, ISSN 1838-7640
Publisher: Ivyspring International Publisher
DOI: 10.7150/thno.21906

Generation and use of a humanized bone-marrow-ossicle niche for hematopoietic xenotransplantation into mice

Author(s): Andreas Reinisch, David Cruz Hernandez, Katharina Schallmoser, Ravindra Majeti
Published in: Nature Protocols, Issue 12/10, 2017, Page(s) 2169-2188, ISSN 1754-2189
Publisher: Nature Publishing Group
DOI: 10.1038/nprot.2017.088

International Forum on GMP-grade human platelet lysate for cell propagation: summary

Author(s): D. Strunk, M. Lozano, D.C. Marks, Y. S. Loh, G. Gstraunthaler, H. Schennach, E. Rohde, S. Laner-Plamberger, M. Öller, J. Nystedt, R. Lotfi, M. Rojewski, H. Schrezenmeier, K. Bieback, R. Schäfer, T. Bakchoul, M. Waidmann, S.M. Jonsdottir-Buch, H. Montazeri, O.E. Sigurjonsson, P. Iudicone, D. Fioravanti, L. Pierelli, M. Introna, C. Capelli, A. Falanga, M. Takanashi, O. Lόpez-Villar, T. Burnouf, J
Published in: Vox Sanguinis, Issue 113/1, 2018, Page(s) 80-87, ISSN 0042-9007
Publisher: Blackwell Publishing Inc.
DOI: 10.1111/vox.12593

Upregulation of mitotic bookmarking factors during enhanced proliferation of human stromal cells in human platelet lysate

Author(s): Sandra Laner-Plamberger, Michaela Oeller, Cornelia Mrazek, Arnulf Hartl, Alina Sonderegger, Eva Rohde, Dirk Strunk, Katharina Schallmoser
Published in: Journal of Translational Medicine, Issue 17/1, 2019, ISSN 1479-5876
Publisher: BioMed Central
DOI: 10.1186/s12967-019-02183-0

Heparin Differentially Impacts Gene Expression of Stromal Cells from Various Tissues

Author(s): Sandra Laner-Plamberger, Michaela Oeller, Rodolphe Poupardin, Linda Krisch, Sarah Hochmann, Ravi Kalathur, Karin Pachler, Christina Kreutzer, Gerrit Erdmann, Eva Rohde, Dirk Strunk, Katharina Schallmoser
Published in: Scientific Reports, Issue 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-43700-x

A novel waterjet technology for transurethral cystoscopic injection of viable cells in the urethral sphincter complex

Author(s): Luise Jäger, Walter Linzenbold, Andreas Fech, Markus Enderle, Tanja Abruzzese, Arnulf Stenzl, Wilhelm K. Aicher
Published in: Neurourology and Urodynamics, Issue 39/2, 2019, Page(s) 594-602, ISSN 0733-2467
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/nau.24261

Platelet-derived factors impair placental chorionic gonadotropin beta-subunit synthesis

Author(s): Désirée Forstner, Sabine Maninger, Olivia Nonn, Jacqueline Guettler, Gerit Moser, Gerd Leitinger, Elisabeth Pritz, Dirk Strunk, Katharina Schallmoser, Gunther Marsche, Akos Heinemann, Berthold Huppertz, Martin Gauster
Published in: Journal of Molecular Medicine, Issue 98/2, 2020, Page(s) 193-207, ISSN 0946-2716
Publisher: Springer Verlag
DOI: 10.1007/s00109-019-01866-x

Extracellular vesicles from human multipotent stromal cells protect against hearing loss after noise trauma in vivo

Author(s): Athanasia Warnecke, Jennifer Harre, Hinrich Staecker, Nils Prenzler, Dirk Strunk, Sebastien Couillard‐Despres, Pasquale Romanelli, Julia Hollerweger, Teresa Lassacher, Daniela Auer, Karin Pachler, Georg Wietzorrek, Ulrike Köhl, Thomas Lenarz, Katharina Schallmoser, Sandra Laner‐Plamberger, Christine S. Falk, Eva Rohde, Mario Gimona
Published in: Clinical and Translational Medicine, Issue 10/8, 2020, ISSN 2001-1326
Publisher: Springer Verlag
DOI: 10.1002/ctm2.262