Setting an interdisciplinary/sectorial/international research network to explore dry storage as an alternative strategy for cells/germplasm biobanking

Deliverables

Documents, reports (2)

Technical report on structural and functional characterisation of desiccated of desiccated hiPSCs, fibroblasts, oocytes and embryos

Dissemination and Communication strategy

Websites, patent fillings, videos etc. (1)

logo, Webpage, social media tools, newsletters

Publications

Peer reviewed articles (27)

Controlled spermatozoa-oocyte interaction improves embryo quality in sheep.

Author(s): Anzalone DA, Palazzese L, Czernik M, Sabatucci A, Valbonetti L, Capra E, Loi P.
Published in: Scientific Reports (Sci Rep), 2021, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-021-02000-z

25th ANNIVERSARY OF CLONING BY SOMATIC-CELL NUCLEAR TRANSFER: Scientific and technological approaches to improve SCNT efficiency in farm animals and pets

Author(s): Loi P, Palazzese L, Scapolo PA, Fulka J, Fulka H, Czernik M.
Published in: Reproduction, 2021, ISSN 1741-7899
Publisher: Society for Reproduction and Fertility
DOI: 10.1530/rep-20-0653

Effects of Trichostatin A on the Timing of the First Cleavage and In Vitro Developmental Potential of Bovine Somatic Cell Nuclear Transfer Embryos

Author(s): Satoshi Akagi, Kazutsugu Matsukawa
Published in: Cellular Reprogramming, 21524971, 2022, ISSN 2152-4971
Publisher: Mary Ann Liebert Inc.
DOI: 10.1089/cell.2022.0003

Embryos and embryonic stem cells from the white rhinoceros.

Author(s): Hildebrandt TB, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree MB, Stejskal J, Hayashi K, Drukker M, Loi P, Göritz F, Lazzari G, Galli C.
Published in: Nature Communications (Nat Commun), 9/1, 2018, Page(s) 2589, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-018-04959-2

Insights into the Interaction Between Lipid Bilayers and Trehalose Aqueous Solutions.

Author(s): A. Kumar, A. Cincotti, S. Aparicio
Published in: Journal of Molecular Liquids, 314, 2020, Page(s) 113639, ISSN 0167-7322
Publisher: Elsevier BV
DOI: 10.1016/j.molliq.2020.113639

Effects of Trichostatin A on the Timing of the First Cleavage and In Vitro Developmental Potential of Bovine Somatic Cell Nuclear Transfer Embryos. 

Author(s): Satoshi Akagi , Shinnosuke Tamura, Kazutsugu Matsukawa
Published in: Cellular Reprogramming, 21524971, 2020, ISSN 2152-4971
Publisher: Mary Ann Liebert Inc.
DOI: 10.1089/cell.2019.0074

Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report.

Author(s): Rychtarova J, Langerova A, Fulka H, Loi P, Benc M, Fulka J Jr.
Published in: Animals, 2021, ISSN 2076-2615
Publisher: Molecular Diversity Preservation International
DOI: 10.3390/ani11051250

Whole genome integrity and enhanced developmental potential in ram freeze-dried spermatozoa at mild sub-zero temperature.

Author(s): Palazzese L, Anzalone DA, Turri F, Faieta M, Donnadio A, Pizzi F, Pittia P, Matsukawa K, Loi P.
Published in: Scientific Reports (Sci Rep), 10/1, 2020, Page(s) 18873, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-020-76061-x

A Theoretical Study on Trehalose + Water Mixtures for Dry Preservation Purposes.

Author(s): Amit Kumar; Alberto Cincotti; Santiago Aparicio
Published in: Issue 6, 14203049, 2020, ISSN 1420-3049
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/molecules25061435

hMSCs in contact with DMSO for cryopreservation: experiments and modelling of osmotic injury and cytotoxic effect.

Author(s): G. Traversari, F. Delogu, S. Aparicio, A. Cincotti
Published in: Biotechnology and Bioengineering, 119/10, 2022, Page(s) 2890-2907, ISSN 1097-0290
Publisher: Wiley Online Library
DOI: 10.1002/bit.28174

Oocyte Cryopreservation in Domestic Animals and Humans: Principles, Techniques and Updated Outcomes

Author(s): Tharasanit, T., Thuwanut, P.
Published in: Animals, 11/10, 2021, Page(s) 2949, ISSN 2076-2615
Publisher: Molecular Diversity Preservation International
DOI: 10.3390/ani11102949

Freeze-dried spermatozoa: An alternative biobanking option for endangered species

Author(s): Debora Agata Anzalone, Luca Palazzese, Domenico Iuso, Giuseppe Martino, Pasqualino Loi
Published in: Animal Reproduction Science, 190, 2018, Page(s) 85-93, ISSN 0378-4320
Publisher: Elsevier BV
DOI: 10.1016/j.anireprosci.2018.01.010

Nuclear quiescence and histone hyper-acetylation jointly improve protamine-mediated nuclear remodeling in sheep fibroblasts

Author(s): Luca Palazzese, Marta Czernik, Domenico Iuso, Paola Toschi, Pasqualino Loi
Published in: PLOS ONE, 13/3, 2018, Page(s) e0193954, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0193954

Exploring dry storage as an alternative biobanking strategy inspired by Nature

Author(s): Joseph Saragusty, Pasqualino Loi
Published in: Theriogenology, 126, 2019, Page(s) 17-27, ISSN 0093-691X
Publisher: Elsevier BV
DOI: 10.1016/j.theriogenology.2018.11.027

Transcriptome analysis of the anhydrobiotic cell line Pv11 infers the mechanism of desiccation tolerance and recovery

Author(s): Takahiro G. Yamada, Yoshitaka Suetsugu, Ruslan Deviatiiarov, Oleg Gusev, Richard Cornette, Alexander Nesmelov, Noriko Hiroi, Takahiro Kikawada, Akira Funahashi
Published in: Scientific Reports, 8/1, 2018, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-018-36124-6

DNA fragmentation in epididymal freeze-dried ram spermatozoa impairs embryo development

Author(s): Luca PALAZZESE, Jaime GOSÁLVEZ, Debora A. ANZALONE, Pasqualino LOI, Joseph SARAGUSTY
Published in: Journal of Reproduction and Development, 64/5, 2018, Page(s) 393-400, ISSN 0916-8818
Publisher: IPEC Inc.
DOI: 10.1262/jrd.2018-033

Maternal peri-conceptional undernourishment perturbs offspring sperm methylome

Author(s): Paola Toschi, Emanuele Capra, Debora A Anzalone, Barbara Lazzari, Federica Turri, Flavia Pizzi, Pier A Scapolo, Alessandra Stella, John L Williams, Paolo Ajmone Marsan, Pasqualino Loi
Published in: Reproduction, 159/5, 2020, Page(s) 513-523, ISSN 1470-1626
Publisher: Society for Reproduction and Fertility
DOI: 10.1530/rep-19-0549

Somatic cell nuclear transfer: failures, successes and the challenges ahead

Author(s): Marta Czernik, Debora A. Anzalone, Luca Palazzese, Mami Oikawa, Pasqualino Loi
Published in: The International Journal of Developmental Biology, 63/3-4-5, 2019, Page(s) 123-130, ISSN 0214-6282
Publisher: University of the Basque Country Press
DOI: 10.1387/ijdb.180324mc

Identification of a novel strong promoter from the anhydrobiotic midge, Polypedilum vanderplanki, with conserved function in various insect cell lines

Author(s): Yugo Miyata, Shoko Tokumoto, Yoichiro Sogame, Ruslan Deviatiiarov, Jun Okada, Richard Cornette, Oleg Gusev, Elena Shagimardanova, Minoru Sakurai, Takahiro Kikawada
Published in: Scientific Reports, 9/1, 2019, ISSN 2045-2322
Publisher: Nature Publishing Group
DOI: 10.1038/s41598-019-43441-x

Dry biobanking as a conservation tool in the Anthropocene

Author(s): Joseph Saragusty, Debora Agata Anzalone, Luca Palazzese, Amir Arav, Pasquale Patrizio, Jaime Gosálvez, Pasqualino Loi
Published in: Theriogenology, 2020, ISSN 0093-691X
Publisher: Elsevier BV
DOI: 10.1016/j.theriogenology.2020.01.022

Corrigendum to “Freeze-dried spermatozoa: An alternative biobanking option for endangered species” [Anim. Reprod. Sci. 190C (2018) 85–93]

Author(s): Debora Agata Anzalone, Luca Palazzese, Domenico Iuso, Giuseppe Martino, Pasqualino Loi
Published in: Animal Reproduction Science, 202, 2019, Page(s) 96, ISSN 0378-4320
Publisher: Elsevier BV
DOI: 10.1016/j.anireprosci.2018.12.008

Late Embryogenesis Abundant (LEA) proteins confer water stress tolerance to mammalian somatic cells

Author(s): M. Czernik, A. Fidanza, F.P. Luongo, L. Valbonetti, P.A. Scapolo, P. Patrizio, P. Loi
Published in: Cryobiology, 2020, ISSN 0011-2240
Publisher: Academic Press
DOI: 10.1016/j.cryobiol.2020.01.009

Modelling the osmotic behaviour of human mesenchymal stem cells

Author(s): Elisa Casula, Gabriele Traversari, Sarah Fadda, Oleksiy V. Klymenko, Cleo Kontoravdi, Alberto Cincotti
Published in: Biochemical Engineering Journal, 151, 2019, Page(s) 107296, ISSN 1369-703X
Publisher: Elsevier BV
DOI: 10.1016/j.bej.2019.107296

Insights into the model of non-perfect osmometer cells for cryopreservation: A parametric sweep analysis

Author(s): G. Traversari, A. Cincotti
Published in: Cryobiology, 100, 2021, Page(s) 193-211, ISSN 0011-2240
Publisher: Academic Press
DOI: 10.1016/j.cryobiol.2020.11.013

Long-term storage of gametes and gonadal tissues at room temperatures: the end of the ice age?

Author(s): Comizzoli P, Loi P, Patrizio P, Hubel A.
Published in: Journal of Assisted Reproduction and Genetics, 2022, ISSN 1058-0468
Publisher: Kluwer Academic/Plenum Publishers
DOI: 10.1007/s10815-021-02392-x

The ART of bringing extinction to a freeze - History and future of species conservation, exemplified by rhinos.

Author(s): Hildebrandt TB, Hermes R, Goeritz F, Appeltant R, Colleoni S, de Mori B, Diecke S, Drukker M, Galli C, Hayashi K, Lazzari G, Loi P, Payne J, Renfree M, Seet S, Stejskal J, Swegen A, Williams SA, Zainuddin ZZ, Holtze S.
Published in: Theriogenology, 2021, ISSN 0093-691X
Publisher: Elsevier BV
DOI: 10.1016/j.theriogenology.2021.04.006

Identification of Genomic Safe Harbors in the Anhydrobiotic Cell Line, Pv11.

Author(s): Miyata Y, Tokumoto S, Arai T, Shaikhutdinov N, Deviatiiarov R, Fuse H, Gogoleva N, Garushyants S, Cherkasov A, Ryabova A, Gazizova G, Cornette R, Shagimardanova E, Gusev O, Kikawada T.
Published in: Genes (Basel), 2022, ISSN 2073-4425
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes13030406.