Setting an interdisciplinary/sectorial/international research network to explore dry storage as an alternative strategy for cells/germplasm biobanking

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Technical report on structural and functional characterisation of desiccated of desiccated hiPSCs, fibroblasts, oocytes and embryos

Dissemination and Communication strategy

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Pubblicazioni

Controlled spermatozoa-oocyte interaction improves embryo quality in sheep.

Autori: Anzalone DA, Palazzese L, Czernik M, Sabatucci A, Valbonetti L, Capra E, Loi P.
Pubblicato in: Scientific Reports (Sci Rep), 2021, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-021-02000-z

25th ANNIVERSARY OF CLONING BY SOMATIC-CELL NUCLEAR TRANSFER: Scientific and technological approaches to improve SCNT efficiency in farm animals and pets

Autori: Loi P, Palazzese L, Scapolo PA, Fulka J, Fulka H, Czernik M.
Pubblicato in: Reproduction, 2021, ISSN 1741-7899
Editore: Society for Reproduction and Fertility
DOI: 10.1530/rep-20-0653

Embryos and embryonic stem cells from the white rhinoceros.

Autori: Hildebrandt TB, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree MB, Stejskal J, Hayashi K, Drukker M, Loi P, Göritz F, Lazzari G, Galli C.
Pubblicato in: Nature Communications (Nat Commun), Numero 9/1, 2018, Pagina/e 2589, ISSN 2041-1723
Editore: Nature Publishing Group
DOI: 10.1038/s41467-018-04959-2

Insights into the Interaction Between Lipid Bilayers and Trehalose Aqueous Solutions.

Autori: A. Kumar, A. Cincotti, S. Aparicio
Pubblicato in: Journal of Molecular Liquids, Numero 314, 2020, Pagina/e 113639, ISSN 0167-7322
Editore: Elsevier BV
DOI: 10.1016/j.molliq.2020.113639

Interspecific ICSI for the Assessment of Sperm DNA Damage: Technology Report.

Autori: Rychtarova J, Langerova A, Fulka H, Loi P, Benc M, Fulka J Jr.
Pubblicato in: Animals, 2021, ISSN 2076-2615
Editore: Molecular Diversity Preservation International
DOI: 10.3390/ani11051250

Whole genome integrity and enhanced developmental potential in ram freeze-dried spermatozoa at mild sub-zero temperature.

Autori: Palazzese L, Anzalone DA, Turri F, Faieta M, Donnadio A, Pizzi F, Pittia P, Matsukawa K, Loi P.
Pubblicato in: Scientific Reports (Sci Rep), Numero 10/1, 2020, Pagina/e 18873, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-020-76061-x

hMSCs in contact with DMSO for cryopreservation: experiments and modelling of osmotic injury and cytotoxic effect.

Autori: G. Traversari, F. Delogu, S. Aparicio, A. Cincotti
Pubblicato in: Biotechnology and Bioengineering, Numero 119/10, 2022, Pagina/e 2890-2907, ISSN 1097-0290
Editore: Wiley Online Library
DOI: 10.1002/bit.28174

Oocyte Cryopreservation in Domestic Animals and Humans: Principles, Techniques and Updated Outcomes

Autori: Tharasanit, T., Thuwanut, P.
Pubblicato in: Animals, Numero 11/10, 2021, Pagina/e 2949, ISSN 2076-2615
Editore: Molecular Diversity Preservation International
DOI: 10.3390/ani11102949

Freeze-dried spermatozoa: An alternative biobanking option for endangered species

Autori: Debora Agata Anzalone, Luca Palazzese, Domenico Iuso, Giuseppe Martino, Pasqualino Loi
Pubblicato in: Animal Reproduction Science, Numero 190, 2018, Pagina/e 85-93, ISSN 0378-4320
Editore: Elsevier BV
DOI: 10.1016/j.anireprosci.2018.01.010

Nuclear quiescence and histone hyper-acetylation jointly improve protamine-mediated nuclear remodeling in sheep fibroblasts

Autori: Luca Palazzese, Marta Czernik, Domenico Iuso, Paola Toschi, Pasqualino Loi
Pubblicato in: PLOS ONE, Numero 13/3, 2018, Pagina/e e0193954, ISSN 1932-6203
Editore: Public Library of Science
DOI: 10.1371/journal.pone.0193954

Exploring dry storage as an alternative biobanking strategy inspired by Nature

Autori: Joseph Saragusty, Pasqualino Loi
Pubblicato in: Theriogenology, Numero 126, 2019, Pagina/e 17-27, ISSN 0093-691X
Editore: Elsevier BV
DOI: 10.1016/j.theriogenology.2018.11.027

Transcriptome analysis of the anhydrobiotic cell line Pv11 infers the mechanism of desiccation tolerance and recovery

Autori: Takahiro G. Yamada, Yoshitaka Suetsugu, Ruslan Deviatiiarov, Oleg Gusev, Richard Cornette, Alexander Nesmelov, Noriko Hiroi, Takahiro Kikawada, Akira Funahashi
Pubblicato in: Scientific Reports, Numero 8/1, 2018, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-018-36124-6

DNA fragmentation in epididymal freeze-dried ram spermatozoa impairs embryo development

Autori: Luca PALAZZESE, Jaime GOSÁLVEZ, Debora A. ANZALONE, Pasqualino LOI, Joseph SARAGUSTY
Pubblicato in: Journal of Reproduction and Development, Numero 64/5, 2018, Pagina/e 393-400, ISSN 0916-8818
Editore: IPEC Inc.
DOI: 10.1262/jrd.2018-033

Maternal peri-conceptional undernourishment perturbs offspring sperm methylome

Autori: Paola Toschi, Emanuele Capra, Debora A Anzalone, Barbara Lazzari, Federica Turri, Flavia Pizzi, Pier A Scapolo, Alessandra Stella, John L Williams, Paolo Ajmone Marsan, Pasqualino Loi
Pubblicato in: Reproduction, Numero 159/5, 2020, Pagina/e 513-523, ISSN 1470-1626
Editore: Society for Reproduction and Fertility
DOI: 10.1530/rep-19-0549

Somatic cell nuclear transfer: failures, successes and the challenges ahead

Autori: Marta Czernik, Debora A. Anzalone, Luca Palazzese, Mami Oikawa, Pasqualino Loi
Pubblicato in: The International Journal of Developmental Biology, Numero 63/3-4-5, 2019, Pagina/e 123-130, ISSN 0214-6282
Editore: University of the Basque Country Press
DOI: 10.1387/ijdb.180324mc

Identification of a novel strong promoter from the anhydrobiotic midge, Polypedilum vanderplanki, with conserved function in various insect cell lines

Autori: Yugo Miyata, Shoko Tokumoto, Yoichiro Sogame, Ruslan Deviatiiarov, Jun Okada, Richard Cornette, Oleg Gusev, Elena Shagimardanova, Minoru Sakurai, Takahiro Kikawada
Pubblicato in: Scientific Reports, Numero 9/1, 2019, ISSN 2045-2322
Editore: Nature Publishing Group
DOI: 10.1038/s41598-019-43441-x

Dry biobanking as a conservation tool in the Anthropocene

Autori: Joseph Saragusty, Debora Agata Anzalone, Luca Palazzese, Amir Arav, Pasquale Patrizio, Jaime Gosálvez, Pasqualino Loi
Pubblicato in: Theriogenology, 2020, ISSN 0093-691X
Editore: Elsevier BV
DOI: 10.1016/j.theriogenology.2020.01.022

Late Embryogenesis Abundant (LEA) proteins confer water stress tolerance to mammalian somatic cells

Autori: M. Czernik, A. Fidanza, F.P. Luongo, L. Valbonetti, P.A. Scapolo, P. Patrizio, P. Loi
Pubblicato in: Cryobiology, 2020, ISSN 0011-2240
Editore: Academic Press
DOI: 10.1016/j.cryobiol.2020.01.009

Modelling the osmotic behaviour of human mesenchymal stem cells

Autori: Elisa Casula, Gabriele Traversari, Sarah Fadda, Oleksiy V. Klymenko, Cleo Kontoravdi, Alberto Cincotti
Pubblicato in: Biochemical Engineering Journal, Numero 151, 2019, Pagina/e 107296, ISSN 1369-703X
Editore: Elsevier BV
DOI: 10.1016/j.bej.2019.107296

Insights into the model of non-perfect osmometer cells for cryopreservation: A parametric sweep analysis

Autori: G. Traversari, A. Cincotti
Pubblicato in: Cryobiology, Numero 100, 2021, Pagina/e 193-211, ISSN 0011-2240
Editore: Academic Press
DOI: 10.1016/j.cryobiol.2020.11.013

Long-term storage of gametes and gonadal tissues at room temperatures: the end of the ice age?

Autori: Comizzoli P, Loi P, Patrizio P, Hubel A.
Pubblicato in: Journal of Assisted Reproduction and Genetics, 2022, ISSN 1058-0468
Editore: Kluwer Academic/Plenum Publishers
DOI: 10.1007/s10815-021-02392-x

The ART of bringing extinction to a freeze - History and future of species conservation, exemplified by rhinos.

Autori: Hildebrandt TB, Hermes R, Goeritz F, Appeltant R, Colleoni S, de Mori B, Diecke S, Drukker M, Galli C, Hayashi K, Lazzari G, Loi P, Payne J, Renfree M, Seet S, Stejskal J, Swegen A, Williams SA, Zainuddin ZZ, Holtze S.
Pubblicato in: Theriogenology, 2021, ISSN 0093-691X
Editore: Elsevier BV
DOI: 10.1016/j.theriogenology.2021.04.006

Identification of Genomic Safe Harbors in the Anhydrobiotic Cell Line, Pv11.

Autori: Miyata Y, Tokumoto S, Arai T, Shaikhutdinov N, Deviatiiarov R, Fuse H, Gogoleva N, Garushyants S, Cherkasov A, Ryabova A, Gazizova G, Cornette R, Shagimardanova E, Gusev O, Kikawada T.
Pubblicato in: Genes (Basel), 2022, ISSN 2073-4425
Editore: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/genes13030406.

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