To uncover enzyme candidates, the approach consisted in the exploration of the enzyme composition of secretomes produced when fungi are cultured on lignocellulosic substrates. The exploration comprised genomic, transcriptomic and proteomic data analyses. One of the major results of Artful is the identification of new families of proteins of which nothing was known before the project. They were identified in the enzyme cocktails produced by cellulolytic fungi. Following their discovery, several proteins of these new families were cloned and produced to evaluate their exact activity. The biochemical characterization that was performed revealed an oxidative mode of action, which is copper-dependent and confirmed the activity of these enzymes, classified thereby as Lytic Polysaccharide MonoOxygenases (LPMOs). Also, the Artful project enabled to extend the study of these enzymes to their biological role, the first of its kind in the LPMO field. The benefits of the exploitation of R&D results are patented (n° EP18306418). Also, The Artful project enable me to attend several Scientific Conferences:
- GRC on Carbohydrate-active Enzymes for Glycan Conversions, Andover (USA), July 2019. Invited speaker.
- 13th Carbohydrate Bioengineering Meeting, Toulouse (France), May 2019. Poster
- 2nd LPMO Symposium, Marseille (France), November 2018. Oral presentation
- 14th European Conference on Fungal Genetics (ECFG14) Haifa (Israël), February 2018. Oral presentation