Objective This project aims to identify the biological species of origin, as well as molecular damage, of proteins used as binding media in ancient Egypt painted artifacts and architecture specimens dating 3000 BC - 600 AD by applying mass spectrometry-based proteomic strategies.A wide part of the artistic production of ancient Egypt civilization is surprisingly still available to us and it is carefully preserved in Egyptian collections spread worldwide. Proteins represent a widely used category of paint binders in ancient Egypt but, at present, only few analyses have been undertaken, leaving several questions open such as: were different proteins preferred for different applications? Did they differ according to period and geographical area? How did proteins in paint formulations modify over millennia? Protein-based samples from ancient Egyptian painted objects will be analysed using state-of-the-art proteomics strategies by high-resolution mass spectrometry. A first screening of the samples will be performed at the British Museum while bottom-up, top-down and quantitative proteomic approaches will be accomplished at the Centre for GeoGenetics. The correct identification of the proteinaceous paint binder, as well as of its biochemical damage, will contribute to understand the artistic technology and to develop proper conservation treatments compatible with the original materials to preserve our cultural heritage for future generations. The Researcher’s skill in analytical chemistry for the investigation of cultural heritage materials, as well as her network with museums and curatorial departments, will be beneficial to the host institution, especially because of the ‘TEMPERA’ European Training Networking coordinated by the main supervisor. At the same time, by receiving advanced, dedicated, research based training on ancient proteomics, her expertise will be significantly enriched, with clear benefits for her future professional career. Fields of science natural sciencescomputer and information sciencessoftwarenatural sciencesbiological sciencesbiochemistrybiomoleculesproteinsproteomicshumanitieshistory and archaeologyhistoryancient historynatural sciencesphysical sciencesopticsspectroscopyabsorption spectroscopynatural scienceschemical sciencesanalytical chemistrymass spectrometry Keywords Cultural heritage conservation proteomics ancient Egypt polychromy science nanoLC-MS MS wall painting mummy portraits Programme(s) H2020-EU.1.3. - EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions Main Programme H2020-EU.1.3.2. - Nurturing excellence by means of cross-border and cross-sector mobility Topic(s) MSCA-IF-2016 - Individual Fellowships Call for proposal H2020-MSCA-IF-2016 See other projects for this call Funding Scheme MSCA-IF - Marie Skłodowska-Curie Individual Fellowships (IF) Coordinator KOBENHAVNS UNIVERSITET Net EU contribution € 200 194,80 Address NORREGADE 10 1165 Kobenhavn Denmark See on map Region Danmark Hovedstaden Byen København Activity type Higher or Secondary Education Establishments Links Contact the organisation Opens in new window Website Opens in new window Participation in EU R&I programmes Opens in new window HORIZON collaboration network Opens in new window Total cost € 200 194,80