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Engineered microbial factories for CO2 exploitation in an integrated waste treatment platform

Objective

The ENGICOIN proposal aims at the development, from TRL3 to TRL5, of three new microbial factories (MFs), integrated in an organic waste anaerobic digestion (AD) platform, based on engineered strains exploiting CO2 sources and renewable solar radiation or H2 for the production of value-added chemicals, namely:
MF.1) the cyanobacteria Synechocystis to produce lactic acid from either biogas combustion flue gases (CO2 concentration ~ 15%) or pure and costless CO2 streams from biogas-to-biomethane purification.
MF.2) the aerobic and toxic metal tolerant Ralstonia eutropha to produce PHA bioplastics from biogas combustion flue gases and complementary carbon sources derived from the AD digestate.
MF.3) the anaerobic Acetobacterium woodii to produce acetone from the CO2 stream from biogas-to-biomethane purification.
High process integration will be guaranteed by taking advantage of low-grade heat sources (e.g. from cogenerative biogas-fired engine or an tailored PEM electrolyser), exploitable side gas streams (e.g. O2 from electrolysis, CO2 from biomethane purification), low-price electricity produced during night-time by a biogas-fired-engine cogeneration unit or even intensified operation conditions (e.g. up to 10 bars pressure for the anaerobic acetone production bioreactor; led-integrated photo-bioreactor). This is an essential feature, alongside with the high conversion rates enabled by synthetic and systems biology on the above microorganisms, to achieve competitive selling prices for the key target products (1.45 €/kg for lactic acid; 3.5 €/kg for PHA; 1 €/kg for acetone).
Notwithstading the key application platform (anaerobic biorefinery based on organic wastes) the innovative production processes developed have a great exploitation potential in other application contexts: flue gases from different combustion appliances (e.g. cement kilns), alcoholic fermentation CO2 streams (e.g. lignocelluosic biorefineries, breweries), etc.

Call for proposal

H2020-NMBP-BIO-2017
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Coordinator

FONDAZIONE ISTITUTO ITALIANO DI TECNOLOGIA
Address
Via Morego 30
16163 Genova
Italy
Activity type
Research Organisations
EU contribution
€ 716 250

Participants (11)

PHOTANOL BV
Netherlands
EU contribution
€ 637 750
Address
Science Park 406
1098 XH Amsterdam
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
KRAJATE GMBH
Austria
EU contribution
€ 275 425
Address
Scharitzerstrasse 30
4020 Linz
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
HYSYTECH SRL
Italy
EU contribution
€ 908 606,25
Address
Strada Del Drosso 33 18
10135 Torino
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
HYDROGENICS EUROPE NV
Belgium
EU contribution
€ 627 225
Address
Nijverheidsstraat 48C
2260 Westerlo
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
BIOPOLIS SL
Spain
EU contribution
€ 368 940
Address
Calle Catedratico Agustin Escardino 9
46980 Paterna Valencia
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
ACEA PINEROLESE INDUSTRIALE SPA
Italy
EU contribution
€ 452 000
Address
Via Vigone 42
10064 Pinerolo
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
ASJA AMBIENTE ITALIA SPA
Italy
EU contribution
€ 301 062,50
Address
Corso Vinzaglio 24
10121 Torino
Activity type
Private for-profit entities (excluding Higher or Secondary Education Establishments)
UNIVERSITEIT VAN AMSTERDAM
Netherlands
EU contribution
€ 600 160
Address
Spui 21
1012WX Amsterdam
Activity type
Higher or Secondary Education Establishments
KUNGLIGA TEKNISKA HOEGSKOLAN
Sweden
EU contribution
€ 395 885
Address
Brinellvagen 8
100 44 Stockholm
Activity type
Higher or Secondary Education Establishments
THE UNIVERSITY OF NOTTINGHAM
United Kingdom
EU contribution
€ 1 192 262,50
Address
University Park
NG7 2RD Nottingham
Activity type
Higher or Secondary Education Establishments
AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Spain
EU contribution
€ 511 343,75
Address
Calle Serrano 117
28006 Madrid
Activity type
Research Organisations