MAnufacturing of TUmour-REactive Natural Killer cells

Deliverables

Recruitment meeting

1 - 3 rounds of recruitment meeting performed to select all ESRs.

Recruitment fellows completed

Recruitment fellows completed.

Career plans for fellows

Career plans for all individual fellows drafted according DoA Part B.

Second round second.

Second round second ments performed as outlined in DocA Part B.

Supervisory board

Supervisory board of project established.

6 Winter + Summer Schools

6 Winter and Summer Schools performed at months 8,12, 18, 24, 30 and 36 as outlined in DoA Part B.

First round secondments

First round secondments performed as outlined in DocA Part B.

Final Consortium Meeting

Final Consortium Meeting performed as outlined in DocA Part B.

Improved NK subset

Improved NK subset tested in vitro and suggested to WP4 and WP5.

Stimulating activat. NKR

Stimulating activat NKR evaluated in vitro and suggested to WP4 and WP5

Lessons clinical trials

Available clinical trials evaluated and lessons transmitted to WP4 and WP5.

Fortified NK cells

NK cells fortified by CAR modification or bispecific reagents tested in vitro and suggested to WP4 and WP5

PhD degrees fellows

Successful PhD degrees for all fellows

Scientific publications fellows

Scientific publications fellows obtained according DoA Part B

Public dissemination events

Public dissemination events performed according DoA Part B

Publications

Single-Cell RNA Sequencing of Tumor-Infiltrating

Author(s): Jing Ni, Xi Wang, Ana Stojanovic, Qin Zhang, Marian Wincher, Lea Bühler, Annette Arnold, Margareta P.Correia. Manuel Winkler. Philipp- Sebastian Koch. Veronika Sexl, Thomas Höfer, Adelheid Cerwenka
Published in: Immunity, Issue 52, 2020, Page(s) 1075-1087, ISSN 1097-4180
Publisher: Cell Press
DOI: 10.1016/j.immuni.2020.05.001

CD116+ fetal precursors migrate to the perinatal lung and give rise to human alveolar macrophages

Author(s): Elza Evren, Emma Ringqvist, Jean-Marc Doisne, Anna Thaller, Natalie Sleiers, Richard A. Flavell, James P. Di Santo, Tim Willinger
Published in: Journal of Experimental Medicine, Issue 219, 2021, Page(s) e20210987, ISSN 1540-9538
Publisher: Rockefeller University Press
DOI: 10.1084/jem.20210987

An optimized platform for efficient siRNA delivery into human NK cells

Author(s): Palacios, D., Momayyezi, P., Huhn, O., Ask, E.H., Dunst, J., Malmberg, K.-J. and Hammer, Q.
Published in: Eur. J. Immunol., Issue 52, 2022, Page(s) 1190-1193, ISSN 0014-2980
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/eji.202149710

Comprehensive analysis of nasal polyps reveals a more pronounced type 2 transcriptomic profile of epithelial cells and mast cells in aspirin-exacerbated respiratory disease

Author(s): Christine Bangert, Sergio Villazala Merino, Martin Fahrenberger, Thomas Krausgruber, Wolfgang Michael Bauer, Victoria Stanek, Nicholas James Campion, Tina Bartosik, Tamara Quint, Guenther Regelsberger, Verena Niederberger-Leppin, Christoph Bock, Sven Schneider, Julia Eckl-Dorna
Published in: Frontiers in Immunology, Issue 13, 2022, Page(s) 850494, ISSN 1664-3224
Publisher: Frontiers Media S.A.
DOI: 10.3389/fimmu.2022.850494

Phenotypic and functional characterization of NK cells in αβT-cell and B-cell depleted haplo-HSCT to cure pediatric patients with acute leukemia

Author(s): Raffaella Meazza, Michela Falco, Fabrizio Loiacono, Paolo Canevali, Mariella Della Chiesa, Alice Bertaina, Daria Pagliara, Pietro Merli, Valentina Indio, Federica Galaverna, Mattia Algeri, Francesca Moretta, Natalia Colomar-Carando, Letizia Muccio, Simona Sivori, Andrea Pession, Maria Cristina Mingari, Lorenzo Moretta, Alessandro Moretta, Franco Locatelli§, and Daniela Pende§. (§F.L. and D.P. e
Published in: Cancers, Issue 12, 2020, Page(s) E2187, ISSN 2072-6694
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers12082187

Small interfering RNA delivery into primary human natural killer cells for functional gene analyses

Author(s): Momayyezi, P., Malmberg, K., & Hammer, Q.
Published in: Current Protocols, Issue 2, 2022, Page(s) e613, ISSN 2691-1299
Publisher: John Wiley & Sons Inc.
DOI: 10.1002/cpz1.613

Innate-like NKp30+CD8+ T cells armed with TCR/CAR target tumor heterogeneity

Author(s): Margareta P. Correia, Ana Stojanovic, Winfried S. Wels and Adelheid Cerwenka
Published in: Oncoimmunology, Issue 10, 2021, Page(s) e1973783, ISSN 2162-4011
Publisher: Landes Bioscience
DOI: 10.1080/2162402x.2021.1973783

Exploiting Natural Killer cell engagers to control pediatric B-cell precursor acute lymphoblastic leukemia

Author(s): Colomar-Carando N., Gauthier L., Merli P., Loiacono F., Canevali P., Falco M., Galaverna F., Rossi B., Bosco F., Caratini M., Mingari M-C., Locatelli F., Vivier E., Meazza R.§, Pende D.§. (§ R.M. and D.P. share last authorship).
Published in: Cancer Immunol Res, Issue 10, 2022, Page(s) 291-302, ISSN 2326-6074
Publisher: American Association for Cancer Research Inc.
DOI: 10.1158/2326-6066.cir-21-0843

Human NK cells, their receptors and function

Author(s): Linda Quatrini, Mariella Della Chiesa, Simona Sivori, Maria Cristina Mingari, Daniela Pende D, Lorenzo Moretta
Published in: Eur. J. Immunol., Issue 51, 2021, Page(s) 1566–1579, ISSN 0014-2980
Publisher: John Wiley & Sons Ltd.
DOI: 10.1002/eji.202049028

Genetic Engineering of Natural Killer Cells for Enhanced Antitumor Function.

Author(s): Mantesso, S., Geerts, D., Spanholtz, J., & Kučerová, L.
Published in: Frontiers in Immunology, Issue 11, 2020, Page(s) 607131, ISSN 1664-3224
Publisher: Frontiers Media S.A.
DOI: 10.3389/fimmu.2020.607131

Dichotomous metabolic networks govern human ILC2 proliferation and function

Author(s): Laura Surace, Jean-Marc Doisne, Carys A. Croft, Anna Thaller, Pedro Escoll, Solenne Marie, Natalia Petrosemoli, Vincent Guillemot, Valerie Dardalhon, Davide Topazio, Antonia Cama, Carmen Buchrieser, Naomi Taylor, Ido Amit, Olimpia Musumeci & James P. Di Santo
Published in: Nature Immunology, Issue 22, 2021, Page(s) 1367-1374, ISSN 1529-2908
Publisher: Nature Publishing Group
DOI: 10.1038/s41590-021-01043-8

NK cells under hypoxia: The two faces of vascularization in tumor and pregnancy

Author(s): Irene Garcés Lázaro, Rebecca Kotzur, Ofer Mandelboim and Adelheid Cerwenka
Published in: Front. Immunol., Issue 13, 2022, Page(s) 924775, ISSN 1664-3224
Publisher: Frontiers Media S.A.
DOI: 10.3389/fimmu.2022.924775

NK cells and ILCs in tumor immunotherapy

Author(s): Simona Sivori*, Daniela Pende*, Linda Quatrini*, Gabriella Pietra, Mariella Della Chiesa, Paola Vacca, Nicola Tumino, Francesca Moretta, Maria Cristina Mingari, Franco Locatelli, Lorenzo Moretta
Published in: Mol. Aspects Med, Issue 80, 2020, Page(s) 100870, ISSN 0098-2997
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.mam.2020.100870

Epitope characterization of a monoclonal antibody that selectively recognizes KIR2DL1 allotypes

Author(s): Falco M, Meazza R, Alicata C, Canevali P, Muntasell A, Bottino C, Moretta L, Pende D, Lopez-Botet M.
Published in: HLA, Issue 100, 2022, Page(s) 107-118, ISSN 2059-2310
Publisher: Wiley
DOI: 10.1111/tan.14630

Inhibitory receptors and checkpoints in human NK cells, implications for the immunotherapy of cancer

Author(s): Simona Sivori, Mariella Della Chiesa, Simona Carlomagno, Linda Quatrini, Enrico Munari, Paola Vacca, Nicola Tumino, Francesca Romana Mariotti, Maria Cristina Mingari, Daniela Pende§ and Lorenzo Moretta§. (§D.P. and L.M. equally contributed to this study).
Published in: Front Immunol, Issue 11, 2020, Page(s) 2156, ISSN 1664-3224
Publisher: Frontiers Media S.A.
DOI: 10.3389/fimmu.2020.02156

Notch, RORC and IL-23 signals cooperate to promote multi-lineage human innate lymphoid cell differentiation

Author(s): Carys A. Croft, Anna Thaller, Solenne Marie, Jean-Marc Doisne, Laura Surace, Rui Yang, Anne Puel, Jacinta Bustamante, Jean-Laurent Casanova, James P. Di Sant
Published in: Nature Communications, Issue 13, 2022, Page(s) 4344, ISSN 2041-1723
Publisher: Nature Publishing Group
DOI: 10.1038/s41467-022-32089-3

CAR-Expressing Natural Killer Cells for Cancer Retargeting

Author(s): Kloess S., Kretschmer A., Stahl L., Fricke S., Koehl U.
Published in: Transfus med and hemother, Issue 46, 2019, Page(s) 4-13, ISSN 1424-5493
Publisher: S. Karger AG
DOI: 10.1159/000495771

NK Cell-Based Immunotherapy for Hematological Malignancies

Author(s): Simona Sivori, Raffaella Meazza, Concetta Quintarelli, Simona Carlomagno, Mariella Della Chiesa, Michela Falco, Lorenzo Moretta, Franco Locatelli, Daniela Pende
Published in: J Clin Med, Issue 8, 2019, Page(s) 1702, ISSN 2077-0383
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/jcm8101702

Adaptive single-KIR+NKG2C+ NK cells expanded from select superdonors show potent missing-self reactivity and efficiently control HLA-mismatched acute myeloid leukemia

Author(s): Haroun-Izquierdo, A., Vincenti, M., Netskar, H., van Ooijen, H., Zhang, B., Bendzick, L., Kanaya, M., Momayyezi, P., Li, S., Wiiger, M.T., Hoel, H.J., Krokeide, S.Z., Kremer, V., Tjønnfjord, G., Berglund, S., Wikström, K., Blomberg, P., Alici, E., Felices, M., Önfelt, B., Valamehr, B., Ljunggren, H.G., Björklund, A, Hammer, Q., Kveberg, L., Cichocki, F., Miller, J.S., Malmberg, K-J., and Sohlb
Published in: J. Immunother Cancer, Issue 10, 2022, Page(s) e005577, ISSN 2455-8591
Publisher: Society for Immunotherapy of Cancer - BMJ Journals
DOI: 10.1136/jitc-2022-005577

Harnessing NK Cell Checkpoint-Modulating Immunotherapies

Author(s): Chauhan, S.K.S.; Koehl, U.; Klöß, S.
Published in: Cancers, Issue 12, 2020, Page(s) 1807, ISSN 2072-6694
Publisher: Multidisciplinary Digital Publishing Institute (MDPI)
DOI: 10.3390/cancers12071807

NK-92 cells retain vitality and functionality when grown in standard cell culture conditions

Author(s): Kotzur, R., Duev-Cohen, A., Kol, I., Reches, A., Mandelboim, O.
Published in: Plos One, Issue 17, 2022, Page(s) e0264897, ISSN 1932-6203
Publisher: Public Library of Science
DOI: 10.1371/journal.pone.0264897

Characterization of KIR+ NK cell subsets with a monoclonal antibody selectively recognizing KIR2DL1 and blocking the specific interaction with HLA-C

Author(s): Meazza R.§, Falco M.§, Canevali P., Loiacono F., Colomar-Carando N., Muntasell A., Rea A., Mingari M-C., Locatelli F., Moretta L., Lopez-Botet M.*, Pende D*. (§ R.M. and M.F. share first authorship and * M.L.-B. and D.P. share last authorship).
Published in: HLA, Issue 2022, 2022, Page(s) 1-14, ISSN 2059-2302
Publisher: Wiley Online
DOI: 10.1111/tan.14640

CD137 Costimulation Counteracts TGFβ Inhibition of NK-cell Antitumor Function

Author(s): Mariona Cabo, Sara Santana-Hernández, Marcel Costa-Garcia, Anna Rea, Roberto Lozano-Rodríguez, Michelle Ataya, Francesc Balaguer, Manel Juan, Maria C Ochoa, Silvia Menéndez, Laura Comerma, Ana Rovira, Pedro Berraondo, Joan Albanell, Ignacio Melero, Miguel López-Botet, Aura Muntasell
Published in: Cancer Immunol Res, Issue 9, 2021, Page(s) 1476-1490, ISSN 2326-6074
Publisher: American Association of Cancer Research
DOI: 10.1158/2326-6066.cir-21-0030

Human ILC3 Exert TRAIL-Mediated Cytotoxicity Towards Cancer Cells

Author(s): Jana-Julia Siegler, Margareta P. Correia, Tomáš Hofman, Isabel Prager, Emrullah Birgin, Nuh N. Rahbari, Carsten Watzl, Ana Stojanovic and Adelheid Cerwenka
Published in: Front. Immunol., Issue 13, 2022, Page(s) 742571, ISSN 1664-3224
Publisher: Frontiers Media S.A.
DOI: 10.3389/fimmu.2022.742571

Polarized mitochondria as guardians of NK cell fitness

Author(s): Laura Surace, Jean-Marc Doisne, Pedro Escoll, Solenne Marie, Valerie Dardalhon, Carys Croft, Anna Thaller, Davide Topazio, Angelo Sparaneo, Antonia Cama, Olimpia Musumeci, Aurelio d’Ecclesia, Carmen Buchrieser, Naomi Taylor, James P. Di Santo
Published in: Blood Advances, Issue 5, 2020, Page(s) 26-38, ISSN 2473-9537
Publisher: The American Society of Hematology
DOI: 10.1182/bloodadvances.2020003458

Cellular immunotherapy with multiple infusions of in vitro-expanded haploidentical natural killer cells after autologous transplantation for patients with plasma cell myeloma

Author(s): Tschan-Plessl A, Kalberer CP, Wieboldt R, Stern M, Siegler U, Wodnar-Filipowicz A, Gerull S, Halter J, Heim D, Tichelli A, Tsakiris DA, Malmberg KJ, Passweg JR, Bottos A.
Published in: Cytotherapy, Issue 4, 2021, Page(s) 329-338, ISSN 1465-3249
Publisher: Taylor & Francis
DOI: 10.1016/j.jcyt.2020.09.009

Deliverables

Publications

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