Animal evolution from a cell type perspective: multidisciplinary training in single-cell genomics, evo-devo and in science outreach

Project Information

EvoCELL

Grant agreement ID: 766053

DOI 10.3030/766053

Project closed

EC signature date 30 August 2017

Start date 1 January 2018

End date 30 June 2022

Funded under

• EXCELLENT SCIENCE - Marie Skłodowska-Curie Actions

Total cost € 3 784 635,85

EU contribution € 3 784 635,85

Coordinated by

EUROPEAN MOLECULAR BIOLOGY LABORATORY
Germany

Deliverables

Other (4)

Final exhibition

Combined virtual and real final exhibition

Practical Course II

Practical Course II - Evolutionary Biology & Data Analysis: Bioinformatic and statistical issues; Alternative doctorate careers I

Practical Course I

Practical Course I - Introduction and Data Acquisition: Next generation sequencing, single-cell sequencing approaches

Practical Course III

Practical Course III Evolutionary Developmental Biology and Neurobiology of Marine Models Alternative doctorate careers III

Websites, patent fillings, videos etc. (1)

Website

Set up the project’s communication website

Documents, reports (3)

Best practice catalogue

Best practice catalogue on presentation techniques and methods used in a virtual exhibition

Dissociation and cell (nucleus) capture protocols

Dissociation and cell nucleus capture protocols for diverse organisms

List of supporting material for outreach

List of supporting material being produced for outreach schools museum visitors etc

Publications

Peer reviewed articles (16)

Developmental toxicity of plastic leachates on the sea urchin Paracentrotus lividus

Author(s): Flora Rendell-Bhatti, Periklis Paganos, Anna Pouch, Christopher Mitchell, Salvatore D’Aniello, Brendan J. Godley, Ksenia Pazdro, Maria Ina Arnone, Eva Jimenez-Guri
Published in: Environmental Pollution, Issue 269, 2021, Page(s) 115744, ISSN 0269-7491
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.envpol.2020.115744

"Panarthropod tiptop/teashirt and spalt orthologs and their potential role as ""trunk""-selector genes."

Author(s): Brenda I. Medina-Jiménez; Graham E. Budd; Ralf Janssen
Published in: EvoDevo, Issue 6, 2021, ISSN 2041-9139
Publisher: BioMed Central
DOI: 10.1186/s13227-021-00177-y

Crustacean leg regeneration restores complex microanatomy and cell diversity

Author(s): Almazán A, Çevrim C, Musser JM, Averof M, Paris M
Published in: Science Advances, 2022, ISSN 2375-2548
Publisher: AAAS
DOI: 10.1126/sciadv.abn9823

Post-metamorphic skeletal growth in the sea urchin Paracentrotus lividus and implications for body plan evolution

Author(s): Jeffrey R. Thompson, Periklis Paganos, Giovanna Benvenuto, Maria Ina Arnone, Paola Oliveri
Published in: EvoDevo, Issue 12/1, 2021, ISSN 2041-9139
Publisher: BioMed Central
DOI: 10.1186/s13227-021-00174-1

FISH for All: A Fast and Efficient Fluorescent In situ Hybridization (FISH) Protocol for Marine Embryos and Larvae

Author(s): Periklis Paganos, Filomena Caccavale, Claudia La Vecchia, Enrico D’Aniello, Salvatore D’Aniello and Maria Ina Arnone
Published in: Frontiers in Physiology, 2022, ISSN 1664-042X
Publisher: Frontiers Research Foundation
DOI: 10.3389/fphys.2022.878062

New Neuronal Subtypes With a “Pre-Pancreatic” Signature in the Sea Urchin Stongylocentrotus purpuratus

Author(s): Margherita Perillo, Periklis Paganos, Teresa Mattiello, Maria Cocurullo, Paola Oliveri, Maria I. Arnone
Published in: Frontiers in Endocrinology, Issue 9, 2018, ISSN 1664-2392
Publisher: Frontiers
DOI: 10.3389/fendo.2018.00650

Neuronal coordination of motile cilia in locomotion and feeding

Author(s): Milena Marinković, Jürgen Berger, Gáspár Jékely
Published in: Philosophical Transactions of the Royal Society B: Biological Sciences, Issue 375/1792, 2019, Page(s) 20190165, ISSN 0962-8436
Publisher: Royal Society of London
DOI: 10.1098/rstb.2019.0165

Molecular and Functional Characterization of the Somatic PIWIL1/piRNA Pathway in Colorectal Cancer Cells

Author(s): Assunta Sellitto, Konstantinos Geles, Ylenia D’Agostino, Marisa Conte, Elena Alexandrova, Domenico Rocco, Giovanni Nassa, Giorgio Giurato, Roberta Tarallo, Alessandro Weisz, Francesca Rizzo
Published in: Cells, Issue 8/11, 2019, Page(s) 1390, ISSN 2073-4409
Publisher: MDPI AG
DOI: 10.3390/cells8111390

The Nereid on the rise: Platynereis as a model system

Author(s): B. Duygu Özpolat, Nadine Randel, Elizabeth A. Williams, Luis Alberto Bezares-Calderón, Gabriele Andreatta, Guillaume Balavoine, Paola Y. Bertucci, David E. K. Ferrier, Maria Cristina Gambi, Eve Gazave, Mette Handberg-Thorsager, Jörg Hardege, Cameron Hird, Yu-Wen Hsieh, Jerome Hui, Kevin Nzumbi Mutemi, Stephan Q. Schneider, Oleg Simakov, Hernando M. Vergara, Michel Vervoort, Gáspár Jékely, Kr
Published in: EvoDevo, Issue 12/1, 2021, ISSN 2041-9139
Publisher: BioMed Central
DOI: 10.1186/s13227-021-00180-3

Whole-animal multiplexed single-cell RNA-seq reveals transcriptional shifts across Clytia medusa cell types.

Author(s): Tara Chari, Brandon Weissbourd, Jase Gehring, Anna Ferraioli, Lucas Leclère, Makenna Herl, Fan Gao, Sandra Chevalier, Richard R. Copley, Evelyn Houliston, David J. Anderson, Lior Pachter.
Published in: SCIENCE ADVANCES, 2021, ISSN 2375-2548
Publisher: American Association for the Advancement of Science.
DOI: 10.1126/sciadv.abh1683

Whole-body integration of gene expression and single-cell morphology

Author(s): Hernando M. Vergara, Constantin Pape, Kimberly I. Meechan, Valentyna Zinchenko, Christel Genoud, Adrian A. Wanner, Kevin Nzumbi Mutemi, Benjamin Titze, Rachel M. Templin, Paola Y. Bertucci, Oleg Simakov, Wiebke Dürichen, Pedro Machado, Emily L. Savage, Lothar Schermelleh, Yannick Schwab, Rainer W. Friedrich, Anna Kreshuk, Christian Tischer, Detlev Arendt
Published in: Cell, Issue 184/18, 2021, Page(s) 4819-4837.e22, ISSN 0092-8674
Publisher: Cell Press
DOI: 10.1016/j.cell.2021.07.017

Distinct gene expression dynamics in developing and regenerating limbs

Author(s): C. Sinigaglia, A. Almazán, M. Lebel, M, Sémon. B. Gillet, S. Hughes, E. Edsinger, M. Averof. and M. Paris
Published in: PNAS, 2022, ISSN 1091-6490
Publisher: National Academy of Science
DOI: 10.1073/pnas.2119297119

Comparative Neurobiology of Biogenic Amines in Animal Models in Deuterostomes

Author(s): Enrico D’Aniello, Periklis Paganos, Evgeniya Anishchenko, Salvatore D’Aniello, Maria Ina Arnone
Published in: Frontiers in Ecology and Evolution, Issue 8, 2020, ISSN 2296-701X
Publisher: Frontiers
DOI: 10.3389/fevo.2020.587036

Single cell RNA sequencing of the Strongylocentrotus purpuratus larva reveals the blueprint of major cell types and nervous system of a non-chordate deuterostome.

Author(s): Periklis Paganos; Danila Voronov; Jacob M. Musser; Detlev Arendt; Maria Ina Arnone
Published in: elife, Issue 9, 2021, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.70416

Coup-TF: A maternal factor essential for differentiation along the embryonic axes in the sea urchin Paracentrotus lividus

Author(s): Ioannis Tsironis, Periklis Paganos, Georgia Gouvi, Panagiotis Tsimpos, Andriana Stamopoulou, Maria Ina Arnone, Constantin N. Flytzanis
Published in: Developmental Biology, Issue 475, 2021, Page(s) 131-144, ISSN 0012-1606
Publisher: Academic Press
DOI: 10.1016/j.ydbio.2020.12.012

A chelicerate Wnt gene expression atlas: novel insights into the complexity of arthropod Wnt-patterning.

Author(s): Ralf Janssen; Matthias Pechmann; Natascha Turetzek
Published in: EvoDevo, Issue 4, 2021, ISSN 2041-9139
Publisher: BioMed Central
DOI: 10.1186/s13227-021-00182-1

Thesis and dissertations (2)

Cell Type Diversity During Sea Urchin Development: A Single Cell Approach to Reveal Different Neuronal Types and Their Evolution

Author(s): Paganos, Periklis
Published in: 2021
Publisher: do not aply
DOI: 10.21954/ou.ro.00012e56

Single-cell RNA sequencing as a tool to study panarthropod evolution (Licentiate thesis, comprehensive summary)

Author(s): Brenda Irene Medina Jimenez
Published in: 2021
Publisher: Diva

Non-peer reviewed articles (2)

WIND (Workflow for pIRNAs aNd beyonD): a strategy for in-depth analysis of small RNA-seq data

Author(s): Konstantinos Geles, Domenico Palumbo, Assunta Sellitto, Giorgio Giurato, Eleonora Cianflone, Fabiola Marino, Daniele Torella, Valeria Mirici Cappa, Giovanni Nassa, Roberta Tarallo, Alessandro Weisz, Francesca Rizzo
Published in: F1000Research, Issue 10, 2021, Page(s) 1, ISSN 2046-1402
Publisher: F1000 Research Ltd.
DOI: 10.12688/f1000research.27868.3

Reconstructing the ancestral vertebrate brain using a lamprey neural cell type atlas

Author(s): Francesco Lamanna, Francisca Hervas-Sotomayor, A. Phillip Oel, David Jandzik, Daniel Sobrido-Cameán, Megan L. Martik, Stephen A. Green, Thoomke Brüning, Katharina Mößinger, Julia Schmidt, Celine Schneider, Mari Sepp, Florent Murat, Jeramiah J. Smith, Marianne E. Bronner, María Celina Rodicio, Antón Barreiro-Iglesias, Daniel M. Medeiros, Detlev Arendt, Henrik Kaessmann
Published in: Biorxiv, 2022, ISSN 2692-8205
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.02.28.482278

Book chapters (1)

Methodology for Whole Mount and Fluorescent RNA In Situ Hybridization in Echinoderms: Single, Double, and Beyond

Author(s): Margherita Perillo, Periklis Paganos, Maxwell Spurrell, Maria I. Arnone, Gary M. Wessel
Published in: Developmental Biology of the Sea Urchin and Other Marine Invertebrates - Methods and Protocols, Issue 2219, 2021, Page(s) 195-216, ISBN 978-1-0716-0973-6
Publisher: Springer US
DOI: 10.1007/978-1-0716-0974-3_12