This is about programming evolution by switching between certain combinations of nutrients/substrates along with a simple deterministic model to compute evolution heterotically. This system is an automated continuous-culture device that controls substrate concentration to maintain constant growth inhibition. The device runs at a certain interval and adds either fresh medium with a high substrate concentration (2,4-dinitrotoluene, DNT as the target molecule in our case) or without it. By giving it pulses of DNT and letting it recover we expect that the ROS produced by faulty reactions by suboptimal dioxygenases will diversify the corresponding DNA sequences until a biochemical solution is found.
The genomes of the two bacterial species adopted in this Project (E. colo and Pseudomonas putida) will be [i] erased of instability determinants and other genes encoding functions considered to be irrelevant for the sake of MADONNA and [ii] endowed with new traits to make them withstand the working conditions of the reactions of interest.
This will be based on a fully autonomous chemical robot system that is able to generate random physicochemical inputs in microdroplet scale. The droplet behaviours will then be analysed, and ranked using a fitness function. This process is continued through iterated generations. This allows inspecting how the genotype is modulated through an envirotype to express the phenotype of interest.
Modeling approaches will be adopted to inspect the pluses and minuses of dealing with live —but heavily refactored microbial agents that could propagate through the Biosphere. This will help to determine the needs of containment of the agents at stake. This lower-scale models will help to understand the resilience and optimal yield conditions of designed microorganisms and microbial consortia and create in silico tests of the proposed peribolic reactions.
The reaction conditions that accompany desired carboxylase recations are often different from physiological conditions (defined as pH of approximately 7, aqueous environments, and ambient temperatures) and turnover numbers very low. In order to make such reaction sustainable and to ultimately recruit such reactions into biology, the protein scaffold has to be evolved. Therefore, we will develope an strategy to implement an enzyme engineering trajectory towards a highly efficient Sav-Pd-catalyst for the production of acrylic acid.
We will take the reaction invented chemically (ie CO2 capture with a metalloenzyme) and and run it in the presence of cells using a chemical droplet robot in which the biological system is programmed to generate peptides that can chelate metal ions to generate ArMs that can be symbiotically selected by the system. This links process engineering to the fitness functions and allows the systems to be linked artificially using such a chem-o-biological robot. The link is software and spectroscopy with chemical feedback. The chemicals can acts as mutagens, and also act as selection agents on the peptides expressed.
Reconciliation of geochemical, biological and industrial/human induced global cycles will not only provide a sustainable way of producing and consuming, but will inevitably change the characteristic of the three cycles, diffusing and enlarging the limits of human intervention on the planet. In this report we will ask: what rationale (world view) is behind the projects aim and goal, what other rationales co-exist, which solutions do they provide? What are the ethical dimensions of going forward with the project versus continuing with the unsustainable business-as-usual.
This is about application of complex networks theory to model new metabolic loops brought about by small-scale and large-scale peribolism. Meso- and large-scale models of ecosystems incorporating the NTN reactions set by MADONNA will be produced. Our ecosystem description incorporates both standard habitats as well as the high-energy, fuel-powered counterparts that are defined by large urban centres and industry. By incorporating peribolism, we will transform these type of waste/sink components into new large-scale chemical processes thus deeply changing the global dynamics. To this goal we will develop a set of mathematical and computational tools that provide a rationale for the expected responses of this global metabolism on different scales.
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Author(s): Víctor de Lorenzo
Published in: Life Sciences, Society and Policy, Issue 14/1, 2018, ISSN 2195-7819
Author(s): Gonzalo Durante-Rodríguez, Víctor de Lorenzo, Pablo I. Nikel
Published in: ACS Synthetic Biology, Issue 7/11, 2018, Page(s) 2686-2697, ISSN 2161-5063
Author(s): Özlem Akkaya, Danilo R. Pérez-Pantoja, Belén Calles, Pablo I. Nikel, Víctor de Lorenzo
Published in: mBio, Issue 9/4, 2018, ISSN 2150-7511
Author(s): Tomás Aparicio, Víctor de Lorenzo, Esteban Martínez-García
Published in: Biotechnology Journal, Issue 14/1, 2019, Page(s) 1800483, ISSN 1860-6768
Author(s): Özlem Akkaya, Pablo I. Nikel, Danilo Pérez-Pantoja, Víctor de Lorenzo
Published in: Environmental Microbiology, Issue 21/1, 2019, Page(s) 314-326, ISSN 1462-2912
Author(s): Ricard V. Solé, Raúl Montañez, Salva Duran-Nebreda, Daniel Rodriguez-Amor, Blai Vidiella, Josep Sardanyés
Published in: Royal Society Open Science, Issue 5/7, 2018, Page(s) 180121, ISSN 2054-5703
Author(s): Max Chavarría, Víctor de Lorenzo
Published in: Molecular Microbiology, Issue 109/3, 2018, Page(s) 273-277, ISSN 0950-382X
Author(s): Pavel Dvořák, Víctor de Lorenzo
Published in: Metabolic Engineering, Issue 48, 2018, Page(s) 94-108, ISSN 1096-7176
Author(s): Pablo I. Nikel, Víctor de Lorenzo
Published in: Metabolic Engineering, Issue 50, 2018, Page(s) 142-155, ISSN 1096-7176
Author(s): Blai Vidiella, Josep Sardanyés, Ricard Solé
Published in: Journal of The Royal Society Interface, Issue 15/143, 2018, Page(s) 20180083, ISSN 1742-5689
Author(s): Tomás Aparicio, Víctor Lorenzo, Esteban Martínez‐García
Published in: Microbial Biotechnology, Issue 12/5, 2019, Page(s) 1076-1089, ISSN 1751-7915
Author(s): Esteban Martínez-García, Angel Goñi-Moreno, Bryan Bartley, James McLaughlin, Lucas Sánchez-Sampedro, Héctor Pascual del Pozo, Clara Prieto Hernández, Ada Serena Marletta, Davide De Lucrezia, Guzmán Sánchez-Fernández, Sofía Fraile, Víctor de Lorenzo
Published in: Nucleic Acids Research, Issue 48/D1, 2019, Page(s) D1164-D1170, ISSN 0305-1048
Author(s): Ángeles Hueso‐Gil, Belén Calles, George A. O'Toole, Víctor Lorenzo
Published in: Microbial Biotechnology, Issue 13/1, 2020, Page(s) 263-273, ISSN 1751-7915
Author(s): David Espeso, Esteban Martínez-García, Víctor Lorenzo
Published in: BIO-PROTOCOL, Issue 9/10, 2019, ISSN 2331-8325
Author(s): Angeles Hueso-Gil, Ákos Nyerges, Csaba Pál, Belén Calles, Víctor de Lorenzo
Published in: ACS Synthetic Biology, Issue 9/1, 2019, Page(s) 104-114, ISSN 2161-5063
Author(s): Belén Calles, Ángel Goñi‐Moreno, Víctor Lorenzo
Published in: Molecular Systems Biology, Issue 15/12, 2019, ISSN 1744-4292
Author(s): Tomas Aparicio, Akos Nyerges, István Nagy, Csaba Pal, Esteban Martínez‐García, Víctor Lorenzo
Published in: Environmental Microbiology, Issue 22/1, 2019, Page(s) 45-58, ISSN 1462-2912
Author(s): Nuria Conde-Pueyo, Blai Vidiella, Josep Sardanyés, Miguel Berdugo, Fernando T. Maestre, Victor de Lorenzo, Ricard Solé
Published in: Life, Issue 10/2, 2020, Page(s) 14, ISSN 2075-1729
Author(s): Tomas Aparicio, Akos Nyerges, Esteban Martínez-García, Víctor de Lorenzo
Published in: iScience, Issue 23/3, 2020, Page(s) 100946, ISSN 2589-0042
Author(s): Alberto Sánchez-Pascuala, Lorena Fernández-Cabezón, Víctor de Lorenzo, Pablo I. Nikel
Published in: Metabolic Engineering, Issue 54, 2019, Page(s) 200-211, ISSN 1096-7176
Author(s): Esteban Martínez-García, Víctor de Lorenzo
Published in: Current Opinion in Biotechnology, Issue 59, 2019, Page(s) 111-121, ISSN 0958-1669
Author(s): Juhyun Kim, Angel Goñi‐Moreno, Belén Calles, Víctor de Lorenzo
Published in: Environmental Microbiology, Issue 21/5, 2019, Page(s) 1645-1658, ISSN 1462-2912
Author(s): Víctor de Lorenzo, Hiren Joshi
Published in: Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids: Biodegradation and Bioremediation, 2019, Page(s) 1-15