Deliverables
The history of technological innovations has demonstrated that the early developers hardly could foresee the various ways the technology would be used and implemented later on To open up all possible narratives of the future we want to invite artists in residency to come and learn in our labs about our mission and give them the opportunity to either carry out an artistic interventions or develop some creative new types of applications of the projects technology
Report on stakeholder involvementFor ellaboration of this report we will involve a number of peers and potential users of the technology such as scientists eg conservational biologists chemists biotechnologists geologists maybe even astrobiologists people form industry small medium and large corporations governance regulatory bodies international organisations like IPCC or CBD and representatives from civil society through workshops and interviews to provide a reality check for the proposed technology
Systems-guided catalogue of sites of intervention with agents delivering NTN reactionsA suite of mathematical and computational approaches ranging from phenomenological to mechanistic will be developed for revisiting the principles that govern the function dynamics and evolution of microbial ecosystems and what allows them to accept new members with novel biochemical properties The way towards topdown synthetic ecology will require new algorithms modeldriven analysis tools and gene function annotation
Report on transposon for converting any Gram-negative bacteria in an HFR strainThis will involve a transposon able to mobilize an entire chromosome though conjugation between a donor and a DNAless recipient For this all the mobtra genes involved in conjugal transfer of superpromiscuous plasmid RP4 will be retrieved from their original genetic frame and assembled as an artificial gene cluster in a miniTn5 transposon vector Upon aquisition of such a transposon any strain will acquire the ability of selftransference and therefore will behave as an HFR strain Highfrequency of recombination that will ease the passing of the genomic information among strains with different degrees of NTN reaction evolution
Proposition of containment needs for microbial agents bearing peribolic reactionsModeling approaches will be adopted to inspect the pluses and minuses of dealing with live —but heavily refactored microbial agents that could propagate through the Biosphere. This will help to determine the needs of containment of the agents at stake. This lower-scale models will help to understand the resilience and optimal yield conditions of designed microorganisms and microbial consortia and create in silico tests of the proposed peribolic reactions.
A merged model of biogeochemical, biological and industrial matter/energy flow in EarthThe concept of Industrial Metabolism traditionally refers in a metaphoric fashion to the physical traffic of material and energy resources through the economic systems as long as similarly to biological metabolism they are processed resulting into goods and waste We wish to go beyond mere metaphores and try to develop actual integrated models of matter and energy flows through the Biosphere and how they could be deliberately or accidentally altered by the introduction at various scales of the NTN reactions entertained in MADONNA and others that could be developed with the same technologies
Detailed report a genetically optimized artificial carboxylase based on evolved Sav isoformsThe reaction conditions that accompany desired carboxylase recations are often different from physiological conditions (defined as pH of approximately 7, aqueous environments, and ambient temperatures) and turnover numbers very low. In order to make such reaction sustainable and to ultimately recruit such reactions into biology, the protein scaffold has to be evolved. Therefore, we will develope an strategy to implement an enzyme engineering trajectory towards a highly efficient Sav-Pd-catalyst for the production of acrylic acid.
Automated generation of metalloenzymes and information capture through a fitness functionWe will take the reaction invented chemically (ie CO2 capture with a metalloenzyme) and and run it in the presence of cells using a chemical droplet robot in which the biological system is programmed to generate peptides that can chelate metal ions to generate ArMs that can be symbiotically selected by the system. This links process engineering to the fitness functions and allows the systems to be linked artificially using such a chem-o-biological robot. The link is software and spectroscopy with chemical feedback. The chemicals can acts as mutagens, and also act as selection agents on the peptides expressed.
Summary and impact of public communication activitiesGiven the fundamental nature of the scope of our project we feel the responsibility to also share this with the general public through a number of science communication formats eg series of short video clips dissemination though web and social media Science Cafe With these formats we do not only aim to inform the public but also to listen to their thoughts and feedback which will be collected consider and eventually be addressed by the project team to alow for a two way communication between science and the public
Responsible innovation. Report of ethics, safety and security tasksReconciliation of geochemical, biological and industrial/human induced global cycles will not only provide a sustainable way of producing and consuming, but will inevitably change the characteristic of the three cycles, diffusing and enlarging the limits of human intervention on the planet. In this report we will ask: what rationale (world view) is behind the projects aim and goal, what other rationales co-exist, which solutions do they provide? What are the ethical dimensions of going forward with the project versus continuing with the unsustainable business-as-usual.
Report on a genetically engineered artificial Fischer-Tropschase enzyme for reduction of CO/CO2 and its expression in vivoGiven that biotinfunctionalized catalysts can be anchored into a streptavidin scaffold this deliverable will report how biotinstreptavidin technology can be exploited selected for incorporation of the Fe4S4 cluster into the genetically encoded protein along with details on characterization of the cofactor and in silico docking studies of the Fe4S4cofactorstreptavidin complex
The aim here is to have the choice of destroying altogether the genetic material of E coli and P putida while preserving the whole of the biochemical networks For this the genes ungdut will be deleted and replaced by a tightly regulated ung dut bicistronic operon inducible upon exposure of cells to an external effector eg 3 methylbenzoate While the lack of ungdut will make many Ts of the genome being replaced by U residues their abrupt expression will cleave the chromosome at multiple sites thus converting the cells in a sort of chemical vesicle These will be excellent vessels to develop NTN reactions and as recipients of heavily mutated and edited artificial chromosomes
Morbidostat for heterotic computation of (bio)chemical problemsThis is about programming evolution by switching between certain combinations of nutrientssubstrates along with a simple deterministic model to compute evolution heterotically This system is an automated continuousculture device that controls substrate concentration to maintain constant growth inhibition The device runs at a certain interval and adds either fresh medium with a high substrate concentration 24dinitrotoluene DNT as the target molecule in our case or without it By giving it pulses of DNT and letting it recover we expect that the ROS produced by faulty reactions by suboptimal dioxygenases will diversify the corresponding DNA sequences until a biochemical solution is found
Genome edited strains of E. coli and P. putida entirely cyborgized for hosting NTN reactionsThe genomes of the two bacterial species adopted in this Project (E. colo and Pseudomonas putida) will be [i] erased of instability determinants and other genes encoding functions considered to be irrelevant for the sake of MADONNA and [ii] endowed with new traits to make them withstand the working conditions of the reactions of interest.
Robotic platform for reverse-reading of chemical reactions into DNAThe coding of DNA in the presence of the process will be reverseread into the system This needs us to generate artificial DNA sequences in the presence of the chemistry and also random peptide synthesis We will build a hybrid digital Complementary metaloxidesemiconductor CMOSdriven synthetic chemical platform to write DNA directly taking advantage of the speed and parallelism of CMOS aiming for GHz write rates on a single strand of DNA within a CMOS nanopore assembler and scale up using PCR this is the reverse of nanopore sequencing
Microdroplet-based environmental simulatorThis will be based on a fully autonomous chemical robot system that is able to generate random physicochemical inputs in microdroplet scale. The droplet behaviours will then be analysed, and ranked using a fitness function. This process is continued through iterated generations. This allows inspecting how the genotype is modulated through an envirotype to express the phenotype of interest.
This Deliverable implies the elaboration of an userfriendly webpage for Communication and Dissemination of Projects activities as well as for internal communication among MADONNA members
This will consist of a document specifying the formats to be followed by the partners for storing the data of interest in a joint repository as well as formal languages for description of actions and constructs
This is about application of complex networks theory to model new metabolic loops brought about by smallscale and largescale peribolism Meso and largescale models of ecosystems incorporating the NTN reactions set by MADONNA will be produced Our ecosystem description incorporates both standard habitats as well as the highenergy fuelpowered counterparts that are defined by large urban centres and industry By incorporating peribolism we will transform these type of wastesink components into new largescale chemical processes thus deeply changing the global dynamics To this goal we will develop a set of mathematical and computational tools that provide a rationale for the expected responses of this global metabolism on different scales
Publications
Author(s):
Napiorkowska, M., Pestalozzi, L., Panke, S., Held, M., & S. Schmitt.
Published in:
Small (Weinheim an der Bergstrasse, Germany),, 2020, ISSN 1613-6829
Publisher:
John Wiley & Sons
DOI:
10.1002/smll.202005523
Author(s):
Tellechea-Luzardo, J., Hobbs, L., Velázquez, E., Pelechova E., Woods, S., de Lorenzo, V. and Krasnogor, N.
Published in:
Nature Communications, 2022, ISSN 2041-1723
Publisher:
Nature Publishing Group
DOI:
10.1038/s41467-022-28350-4
Author(s):
Ting, S.Y., Martinez-Garcia, E., Huang, S., Bertolli, S.K., Kelly, K.A., Cutler, K.J., Su, E.D., Zhi, H., Tang, Q., Radey, M.C., Raffatellu, M., Peterson, S.B., de Lorenzo, V. and Mougous, J.D.
Published in:
Cell Host & Microbe, 2020, ISSN 1934-6069
Publisher:
Elsevier
DOI:
10.1016/j.chom.2020.05.006
Author(s):
Arce-Rodríguez, A., Benedetti I., Borrero-de Acuña, J.M., Silva-Rocha, R., de Lorenzo, V.
Published in:
Synthetic Biology, Issue 23977000, 2021, ISSN 2397-7000
Publisher:
Oxford University Press
DOI:
10.1093/synbio/ysab030
Author(s):
Berdugo, M., Vidiella, B., Solé, R.V. and Maestre, F.T.
Published in:
Functional Ecology, 2021, ISSN 1365-2435
Publisher:
British Ecological Society
DOI:
10.1111/1365-2435.13962
Author(s):
Dvořák, P.; Kováč, Jozef; Lorenzo, Víctor de
Published in:
instname:Consejo Superior de Investigaciones Científicas (CSIC), Issue 17517915, 2020, ISSN 1751-7915
Publisher:
John Wiley & Sons
DOI:
10.1111/1751-7915.13574
Author(s):
Simon Höllerer, Laetitia Papaxanthos, Anja Cathrin Gumpinger, Katrin Fischer, Christian Beisel, Karsten Borgwardt, Yaakov Benenson, Markus Jeschek
Published in:
Nature Communications, Issue 11/1, 2020, ISSN 2041-1723
Publisher:
Nature Publishing Group
DOI:
10.1038/s41467-020-17222-4
Author(s):
Tobias Vornholt, Markus Jeschek
Published in:
ChemBioChem, Issue 21/16, 2020, Page(s) 2241-2249, ISSN 1439-4227
Publisher:
John Wiley & Sons Ltd.
DOI:
10.1002/cbic.202000121
Author(s):
Arce-Rodríguez, A., Nikel, P.I., Calles, B., Chavarría, M., Platero R., Krell, T., de Lorenzo, V.
Published in:
Environmental Biotechnology, 2021, ISSN 1462-2912
Publisher:
Blackwell Publishing Inc.
DOI:
10.1111/1462-2920.15422
Author(s):
Kim, J., Silva-Rocha, R. and de Lorenzo, V.
Published in:
International Microbiology, 2021, ISSN 1139-6709
Publisher:
Sociedad Espanola de Microbiologia
DOI:
10.1007/s10123-021-00194-w
Author(s):
Nediljko Budisa, Vladimir Kubyshkin, Markus Schmidt
Published in:
ChemBioChem, Issue 21/16, 2020, Page(s) 2228-2231, ISSN 1439-4227
Publisher:
John Wiley & Sons Ltd.
DOI:
10.1002/cbic.202000141
Author(s):
Collado-Vides, J., Gaudet, P and de Lorenzo, V.
Published in:
Frontiers in Physiology, 2022, ISSN 1664-042X
Publisher:
Frontiers Research Foundation
DOI:
10.3389/fphys.2022.815874
Author(s):
Apura, R., de Lorenzo, V., Arraiano, C.M., Martínez-García, E. and Viegas S.C.
Published in:
Environmental Microbiology, 2020, ISSN 1462-2912
Publisher:
Blackwell Publishing Inc.
DOI:
10.1111/1462-2920.15291
Author(s):
Espeso, D.R. Dvořák, P., Aparicio, T. de Lorenzo, V.
Published in:
Microbial Biotechnology, 2020, ISSN 1751-7915
Publisher:
John Wiley & Sons
DOI:
10.1111/1751-7915.13678
Author(s):
Dvořák, P., Bayer, E.A., de Lorenzo, V.
Published in:
ACS Synthetic Biology, 2020, ISSN 2161-5063
Publisher:
American Chemical Society
DOI:
10.1021/acssynbio.0c00276
Author(s):
de Lorenzo, V., Krasnogor, N., Schmidt, M.
Published in:
New Biotechnology, 2020, ISSN 1871-6784
Publisher:
Elsevier BV
DOI:
10.1016/j.nbt.2020.08.004
Author(s):
de Lorenzo, V.
Published in:
Metode, 2020, ISSN 2174-9221
Publisher:
Universidad de Valencia
DOI:
10.7203/metode.11.15975
Author(s):
García-Martin, J.A., Chavarría, M., de Lorenzo, V. Pazos, F.
Published in:
Biology Methods and Protocols, 2020, ISSN 2396-8923
Publisher:
Oxford University Press
DOI:
10.1093/biomethods/bpaa025
Author(s):
Vidiella, B. and Solé, R.
Published in:
Iscience, 2022, ISSN 2589-0042
Publisher:
Elsevier
DOI:
10.1016/j.isci.2022.104658
Author(s):
Pérez-Pantoja, D., Nikel, P.I., Chavarría, M. and de Lorenzo, V.
Published in:
Environmental Microbiology, 2021, ISSN 1462-2912
Publisher:
Blackwell Publishing Inc.
DOI:
10.1111/1462-2920.15472
Author(s):
Fraile S., Briones M., Revenga-Parra, M., de Lorenzo V., Lorenzo E. and Martínez-García E
Published in:
ACS Synthetic Biology, 2021, ISSN 2161-5063
Publisher:
American Chemical Society
DOI:
10.1021/acssynbio.1c00227
Author(s):
Dvořák, P., Alvarez-Carreño, C., Paradela, A. and de Lorenzo, V.
Published in:
Environmental Microbiology, 2021, ISSN 1462-2912
Publisher:
Blackwell Publishing Inc.
DOI:
10.1111/1462-2920.15628
Author(s):
Blai Vidiella, Josep Sardanyés, Ricard V. Solé
Published in:
Royal Society Open Science, Issue 7/8, 2020, Page(s) 200161, ISSN 2054-5703
Publisher:
The Royal Society
DOI:
10.1098/rsos.200161
Author(s):
Tas, H., Grozinger, L., Stoof, R., de Lorenzo, V, and Goni-Moreno, A.
Published in:
NAture Communications, 2020, ISSN 2041-1723
Publisher:
Nature Publishing Group
DOI:
10.1038/s41467-020-20656-5
Author(s):
Schmidt, M. & Kubyshkin, V.
Published in:
Chembiochem, 2020, ISSN 1439-4227
Publisher:
John Wiley & Sons Ltd.
DOI:
10.1002/cbic.202000758
Author(s):
de Lorenzo, V.
Published in:
EFB Bioeconomy Journal, 2022, ISSN 2667-0410
Publisher:
Elsevier
DOI:
10.1016/j.bioeco.2022.100035
Author(s):
Víctor de Lorenzo
Published in:
Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences, 2022, ISSN 0962-8436
Publisher:
Royal Society of London
DOI:
10.1098/rstb.2021.0395
Author(s):
Páez-Espino, A.D., Nikel, P., Chavarría, M., de Lorenzo, V.
Published in:
Environmental Microbiology, 2020, ISSN 1462-2912
Publisher:
Blackwell Publishing Inc.
DOI:
10.1111/1462-2920.14991
Author(s):
Nikel, P.I., Fuhrer, T., Chavarría, M., Sánchez-Pascuala, A., Sauer, U. and de Lorenzo, V.
Published in:
ISME Journal, 2020, ISSN 1751-7362
Publisher:
Nature Publishing Group
DOI:
10.1038/s41396-020-00884-9
Author(s):
Al-ramahi, Y., Nyerges, A., Margolles, Y., Cerdán, L., Ferenc G., Pál, C., Fernández, L.A. and de Lorenzo, V.
Published in:
Communications Biology, 2021, ISSN 2399-3642
Publisher:
Nature Communications
DOI:
10.1038/s42003-021-02702-0
Author(s):
T. Vornholt, F. Christoffel, M. M. Pellizzoni, S. Panke, T. R. Ward, M. Jeschek
Published in:
Science Advances, 2022, ISSN 2375-2548
Publisher:
American Association for the Advancement of Science
DOI:
10.1126/sciadv.abe4208
Author(s):
Ángeles Hueso‐Gil, Belén Calles, Víctor Lorenzo
Published in:
Environmental Microbiology, Issue 22/8, 2020, Page(s) 3535-3547, ISSN 1462-2912
Publisher:
Blackwell Publishing Inc.
DOI:
10.1111/1462-2920.15126
Author(s):
Álvarez, B., Mencía, M., de Lorenzo, V., Fernández, LA.
Published in:
Nature Communications, Issue 20411723, 2020, ISSN 2041-1723
Publisher:
Nature Publishing Group
DOI:
10.1038/s41467-020-20230-z
Author(s):
Tas, H., Goni-Moreno, A. and de Lorenzo, V.
Published in:
ACS Synthetic Biology, 2020, ISSN 2161-5063
Publisher:
American Chemical Society
DOI:
10.1021/acssynbio.0c00529
Author(s):
Tobias Vornholt; Fadri Christoffel; Michela M. Pellizzoni; Sven Panke; Thomas R. Ward; Markus Jeschek
Published in:
SCIENCE ADVANCES, Issue 23752548, 2021, ISSN 2375-2548
Publisher:
American Association for the Advancement of Science
DOI:
10.3929/ethz-b-000467617
Author(s):
Velázquez, E., Álvarez, B., Fernández, L.A. and de Lorenzo, V.
Published in:
Microbial Biotechnology, 2022, ISSN 1751-7915
Publisher:
John Wiley & Sons
DOI:
10.1111/1751-7915.14098
Author(s):
Maull, V. and Solé, R.,
Published in:
Philosophical Transactions of the Royal Society B,, 2022, ISSN 0962-8436
Publisher:
Royal Society of London
DOI:
10.1098/rstb.2021.0396
Author(s):
Víctor de Lorenzo
Published in:
Life Sciences, Society and Policy, Issue 14/1, 2018, ISSN 2195-7819
Publisher:
Springer
DOI:
10.1186/s40504-018-0086-x
Author(s):
Gonzalo Durante-Rodríguez, Víctor de Lorenzo, Pablo I. Nikel
Published in:
ACS Synthetic Biology, Issue 7/11, 2018, Page(s) 2686-2697, ISSN 2161-5063
Publisher:
American Chemical Society
DOI:
10.1021/acssynbio.8b00345
Author(s):
Özlem Akkaya, Danilo R. Pérez-Pantoja, Belén Calles, Pablo I. Nikel, Víctor de Lorenzo
Published in:
mBio, Issue 9/4, 2018, ISSN 2150-7511
Publisher:
ASM
DOI:
10.1128/mbio.01512-18
Author(s):
Tomás Aparicio, Víctor de Lorenzo, Esteban Martínez-García
Published in:
Biotechnology Journal, Issue 14/1, 2019, Page(s) 1800483, ISSN 1860-6768
Publisher:
Wiley - VCH Verlag GmbH & CO. KGaA
DOI:
10.1002/biot.201800483
Author(s):
Özlem Akkaya, Pablo I. Nikel, Danilo Pérez-Pantoja, Víctor de Lorenzo
Published in:
Environmental Microbiology, Issue 21/1, 2019, Page(s) 314-326, ISSN 1462-2912
Publisher:
Blackwell Publishing Inc.
DOI:
10.1111/1462-2920.14459
Author(s):
Ricard V. Solé, Raúl Montañez, Salva Duran-Nebreda, Daniel Rodriguez-Amor, Blai Vidiella, Josep Sardanyés
Published in:
Royal Society Open Science, Issue 5/7, 2018, Page(s) 180121, ISSN 2054-5703
Publisher:
Royal Society
DOI:
10.1098/rsos.180121
Author(s):
Max Chavarría, Víctor de Lorenzo
Published in:
Molecular Microbiology, Issue 109/3, 2018, Page(s) 273-277, ISSN 0950-382X
Publisher:
Blackwell Publishing Inc.
DOI:
10.1111/mmi.14080
Author(s):
Pavel Dvořák, Víctor de Lorenzo
Published in:
Metabolic Engineering, Issue 48, 2018, Page(s) 94-108, ISSN 1096-7176
Publisher:
Academic Press
DOI:
10.1016/j.ymben.2018.05.019
Author(s):
Pablo I. Nikel, Víctor de Lorenzo
Published in:
Metabolic Engineering, Issue 50, 2018, Page(s) 142-155, ISSN 1096-7176
Publisher:
Academic Press
DOI:
10.1016/j.ymben.2018.05.005
Author(s):
Blai Vidiella, Josep Sardanyés, Ricard Solé
Published in:
Journal of The Royal Society Interface, Issue 15/143, 2018, Page(s) 20180083, ISSN 1742-5689
Publisher:
The Royal Society
DOI:
10.1098/rsif.2018.0083
Author(s):
Tomás Aparicio, Víctor Lorenzo, Esteban Martínez‐García
Published in:
Microbial Biotechnology, Issue 12/5, 2019, Page(s) 1076-1089, ISSN 1751-7915
Publisher:
John Wiley & Sons
DOI:
10.1111/1751-7915.13453
Author(s):
Esteban Martínez-García, Angel Goñi-Moreno, Bryan Bartley, James McLaughlin, Lucas Sánchez-Sampedro, Héctor Pascual del Pozo, Clara Prieto Hernández, Ada Serena Marletta, Davide De Lucrezia, Guzmán Sánchez-Fernández, Sofía Fraile, Víctor de Lorenzo
Published in:
Nucleic Acids Research, Issue 48/D1, 2019, Page(s) D1164-D1170, ISSN 0305-1048
Publisher:
Oxford University Press
DOI:
10.1093/nar/gkz1024
Author(s):
Ángeles Hueso‐Gil, Belén Calles, George A. O'Toole, Víctor Lorenzo
Published in:
Microbial Biotechnology, Issue 13/1, 2020, Page(s) 263-273, ISSN 1751-7915
Publisher:
Society for Applied Microbiology
DOI:
10.1111/1751-7915.13404
Author(s):
David Espeso, Esteban Martínez-García, Víctor Lorenzo
Published in:
BIO-PROTOCOL, Issue 9/10, 2019, ISSN 2331-8325
Publisher:
Bio-Protocol
DOI:
10.21769/bioprotoc.3238
Author(s):
Angeles Hueso-Gil, Ákos Nyerges, Csaba Pál, Belén Calles, Víctor de Lorenzo
Published in:
ACS Synthetic Biology, Issue 9/1, 2019, Page(s) 104-114, ISSN 2161-5063
Publisher:
American Chemical Society
DOI:
10.1021/acssynbio.9b00375
Author(s):
Belén Calles, Ángel Goñi‐Moreno, Víctor Lorenzo
Published in:
Molecular Systems Biology, Issue 15/12, 2019, ISSN 1744-4292
Publisher:
Nature Publishing Group
DOI:
10.15252/msb.20188777
Author(s):
Tomas Aparicio, Akos Nyerges, István Nagy, Csaba Pal, Esteban Martínez‐García, Víctor Lorenzo
Published in:
Environmental Microbiology, Issue 22/1, 2019, Page(s) 45-58, ISSN 1462-2912
Publisher:
Blackwell Publishing Inc.
DOI:
10.1111/1462-2920.14814
Author(s):
Nuria Conde-Pueyo, Blai Vidiella, Josep Sardanyés, Miguel Berdugo, Fernando T. Maestre, Victor de Lorenzo, Ricard Solé
Published in:
Life, Issue 10/2, 2020, Page(s) 14, ISSN 2075-1729
Publisher:
MDPI
DOI:
10.3390/life10020014
Author(s):
Tomas Aparicio, Akos Nyerges, Esteban Martínez-García, Víctor de Lorenzo
Published in:
iScience, Issue 23/3, 2020, Page(s) 100946, ISSN 2589-0042
Publisher:
Elsevier
DOI:
10.1016/j.isci.2020.100946
Author(s):
Alberto Sánchez-Pascuala, Lorena Fernández-Cabezón, Víctor de Lorenzo, Pablo I. Nikel
Published in:
Metabolic Engineering, Issue 54, 2019, Page(s) 200-211, ISSN 1096-7176
Publisher:
Academic Press
DOI:
10.1016/j.ymben.2019.04.005
Author(s):
Esteban Martínez-García, Víctor de Lorenzo
Published in:
Current Opinion in Biotechnology, Issue 59, 2019, Page(s) 111-121, ISSN 0958-1669
Publisher:
Elsevier BV
DOI:
10.1016/j.copbio.2019.03.012
Author(s):
Juhyun Kim, Angel Goñi‐Moreno, Belén Calles, Víctor de Lorenzo
Published in:
Environmental Microbiology, Issue 21/5, 2019, Page(s) 1645-1658, ISSN 1462-2912
Publisher:
Blackwell Publishing Inc.
DOI:
10.1111/1462-2920.14544
Author(s):
Velázquez, V., Al-Ramahi, Y., Tellechea-Luzardo, J., Krasnogor, N., de Lorenzo, V.
Published in:
ACS Synthetic Biology, 2021, ISSN 2161-5063
Publisher:
American Chemical Society
DOI:
10.1021/acssynbio.1c00199
Author(s):
Espeso D. R., Algar, E., Martínez-García, E., de Lorenzo, V.
Published in:
Exploiting geometric similarity for statistical quantification of fluorescence spatial patterns in bacterial colonies, 2020, ISSN 1471-2105
Publisher:
BioMed Central
DOI:
10.1186/s12859-020-3490-1
Author(s):
Espeso, D.R., Martínez-García, E., de Lorenzo, V.
Published in:
Water Research, 2020, ISSN 0043-1354
Publisher:
Elsevier BV
DOI:
10.1016/j.watres.2020.116468
Author(s):
Markus Schmidt
Published in:
Biosystems, Issue 185, 2019, Page(s) 104015, ISSN 0303-2647
Publisher:
Elsevier BV
DOI:
10.1016/j.biosystems.2019.104015
Author(s):
Tas, H., Grozinger, L., Goni-Moreno, A., de Lorenzo, V.
Published in:
Synthetic Biology, 2021, ISSN 2397-7000
Publisher:
Oxford University Press
DOI:
10.1093/synbio/ysab024
Author(s):
Durante-Rodríguez, G., Páez-Espino, D and de Lorenzo, V.
Published in:
Frontiers in Microbiology, 2021, ISSN 1664-302X
Publisher:
Frontiers Media
DOI:
10.3389/fmicb.2021.641440
Author(s):
Pei, L., Garfinkel, M. & Schmidt, M.
Published in:
Nature Communications, 2022, ISSN 2041-1723
Publisher:
Nature Publishing Group
DOI:
10.1038/s41467-022-29889-y
Author(s):
Kim, J., Goñi-Moreno, A., de Lorenzo, V.
Published in:
mBio, 2021, ISSN 2150-7511
Publisher:
American Society for Microbiology
DOI:
10.1128/mbio.03685-20
Author(s):
Akkaya, Ö., Aparicio, T., Perez-Pantoja, D., de Lorenzo, V.
Published in:
Environmental Microbiology, 2020, ISSN 1462-2912
Publisher:
Blackwell Publishing Inc.
DOI:
10.1111/1462-2920.15384
Author(s):
Miguel Berdugo, Manuel Delgado-Baquerizo, Santiago Soliveres, Rocío Hernández-Clemente, Yanchuang Zhao, Juan J. Gaitán, Nicolas Gross, Hugo Saiz, Vincent Maire, Anika Lehmann, Matthias C. Rillig, Ricard V. Solé, Fernando T. Maestre
Published in:
Science, Issue 367/6479, 2020, Page(s) 787-790, ISSN 0036-8075
Publisher:
American Association for the Advancement of Science
DOI:
10.1126/science.aay5958
Author(s):
Silbert, J., de Lorenzo, V. and Aparicio, T.
Published in:
ACS Synthetic Biology, 2021, ISSN 2161-5063
Publisher:
American Chemical Society
DOI:
10.1021/acssynbio.0c00611
Author(s):
Algar, E., Al-Ramahi, Y., de Lorenzo, V. and Martínez-García, E.
Published in:
ChemBioChem, 2020, ISSN 1439-7633
Publisher:
John Wiley & Sons
DOI:
10.1002/cbic.202000330
Author(s):
Aparicio, T., Silbert, J., Cepeda, S. and de Lorenzo, V.
Published in:
BioDesign Research, 2022, ISSN 2693-1257
Publisher:
American Association for the Advancement of Science
DOI:
10.34133/2022/9850305
Author(s):
Solé, R. and Levin, S.
Published in:
Philosophical Transactions of the Royal Society B,, 2022, ISSN 0962-8436
Publisher:
Royal Society of London
DOI:
10.1098/rstb.2021.0376
Author(s):
Martínez-García, E., Fraile, S., Rodríguez-Espeso, R., Vecchietti, D., Bertoni, G., de Lorenzo, V.
Published in:
ACS Synthetic Biology, 2020, ISSN 2161-5063
Publisher:
American Chemical Society
DOI:
10.1021/acssynbio.0c00272
Author(s):
Nikel, P.I., Benedetti, I., Wirth, N.T., de Lorenzo, V. and Calles, B.
Published in:
Microbial Biotechnology, 2022, ISSN 1751-7915
Publisher:
John Wiley & Sons
DOI:
10.1111/1751-7915.14063
Author(s):
Doran, David; Abul-Haija, Yousef M.; Cronin, Leroy
Published in:
Angewandte Chemie (International Ed. in English), Issue 1, 2019
Publisher:
American Chemical Society (ACS), Chinese Chemical Society, Chemical Society of Japan, German Chemical Society (GDCh) and the Royal Society of Chemistry
DOI:
10.26434/chemrxiv.7712297.v1
Author(s):
Aparicio, T., de Lorenzo, V., Martínez-García, E.
Published in:
Recombineering. Methods in Molecular Biology, 2022, ISBN 978-1-0716-2233-9
Publisher:
Springer Nature
DOI:
10.1007/978-1-0716-2233-9_4
Author(s):
Markus Schmidt, Nediljko Budisa
Published in:
Art as We Don’t Know It, 2020, Page(s) 20-36, ISBN 978-952-60-8823-5
Publisher:
Aalto University Press
Author(s):
Víctor de Lorenzo, Hiren Joshi
Published in:
Consequences of Microbial Interactions with Hydrocarbons, Oils, and Lipids: Biodegradation and Bioremediation, 2019, Page(s) 1-15, ISBN 978-3-319-44535-9
Publisher:
Springer International Publishing
DOI:
10.1007/978-3-319-44535-9_25-1
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