Genetically engineered human pluripotent stem cells, functionalized silk-fibroin platforms and bio-inks: a novel solution for large-scale ex-vivo platelet production, transfusion and drug research

Project Information

SilkFUSION

Grant agreement ID: 767309

DOI

10.3030/767309

Project closed

EC signature date 12 September 2017

Start date 1 November 2017

End date 31 October 2022

Funded under

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Total cost

€ 2 998 500,01

EU contribution

€ 2 998 500,00

Coordinated by

UNIVERSITA DEGLI STUDI DI PAVIA
Italy

CORDIS provides links to public deliverables and publications of HORIZON projects.

Links to deliverables and publications from FP7 projects, as well as links to some specific result types such as dataset and software, are dynamically retrieved from OpenAIRE .

Deliverables

Documents, reports (14)

Scientific papers_24

Summary report of the published scientific papers

Targeted stakeholders identified and informed about the project_36

Links to stakeholders as well as experts on Transfusional Medicine and experts on Bioengineering will be established online and by distributing leaflets, poster and press releases

Scientific papers_12

Summary report of the published scientific papers

Bioengineering a 3D silk platform for large-scale platelet production

Bioengineering the first 3D chemically functionalized silk platform for large-scale production of human platelets

Scientific papers_36

Summary report of the published scientific papers

Targeted stakeholders identified and informed about the project_12

Links to stakeholders as well as experts on Transfusional Medicine and experts on Bioengineering will be established online and by distributing leaflets, poster and press releases

Workshops_12

Summary report of the performed workshops and training sessions

Targeted stakeholders identified and informed about the project_24

Links to stakeholders as well as experts on Transfusional Medicine and experts on Bioengineering will be established online and by distributing leaflets, poster and press releases

Targeted stakeholders identified and informed about the project_48

Links to stakeholders as well as experts on Transfusional Medicine and experts on Bioengineering will be established online and by distributing leaflets poster and press releases

Workshops_36

Summary report of the performed workshops and training sessions

Obtaining a high efficient hPSC lines for producing human platelets for transfusion

A three-level bio-banking system will be applied for the first high efficient hPSC lines

Workshops_48

Summary report of the performed workshops and training sessions

Workshops_24

Summary report of the performed workshops and training sessions

Scientific papers_48

Summary report of the published scientific papers

Websites, patent fillings, videos etc. (2)

Public Project website

Public Project website and logo will be developed and continuously updated

Project video targeting a wide public audience

Project video targeting a wide public audience will be developed

Publications

Peer reviewed articles (12)

Role of Rho-GTPases in megakaryopoiesis

Author(s): William Vainchenker, Brahim Arkoun, Francesca Basso-Valentina, Larissa Lordier, Najet Debili, Hana Raslova
Published in: Small GTPases, 2021, Page(s) 1-17, ISSN 2154-1248
Publisher: Landes Bioscience
DOI: 10.1080/21541248.2021.1885134

Latest culture techniques: cracking the secrets of bone marrow to mass-produce erythrocytes and platelets <i>ex vivo

Author(s): Christian A. Di Buduo, Alicia Aguilar, Paolo M. Soprano, Alberto Bocconi, Carolina P. Miguel, Giovanna Mantica, Alessandra Balduini
Published in: Haematologica, 2020, ISSN 0390-6078
Publisher: Ferrata Storti Foundation
DOI: 10.3324/haematol.2020.262485

Novel variants in GALE cause syndromic macrothrombocytopenia by disrupting glycosylation and thrombopoiesis

Author(s): Marín-Quilez A, Di Buduo CA, Díaz-Ajenjo L, Abbonante V, Vuelta E, Soprano PMM, Miguel-García C, Santos-Mínguez S, Serramito-Gómez I, Ruiz-Sala P, Peñarrubia MJ, Pardal E, Hernández-Rivas JM, González-Porras JR, García-Tuñón I, Benito R, Rivera J, Balduini A, Bastida JM
Published in: Blood, 2022, ISSN 0006-4971
Publisher: American Society of Hematology
DOI: 10.1182/blood.2022016995

Multi-channel silk sponge mimicking bone marrow vascular niche for platelet production

Author(s): Lorenzo Tozzi, Pierre-Alexandre Laurent, Christian A. Di Buduo, Xuan Mu, Angelo Massaro, Ross Bretherton, Whitney Stoppel, David L. Kaplan, Alessandra Balduini
Published in: Biomaterials, Issue 178, 2018, Page(s) 122-133, ISSN 0142-9612
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.biomaterials.2018.06.018

Structurally graduated collagen scaffolds applied to the ex vivo generation of platelets from human pluripotent stem cell-derived megakaryocytes: Enhancing production and purity

Author(s): Jennifer H. Shepherd, Daniel Howard, Amie K. Waller, Holly Rebecca Foster, Annett Mueller, Thomas Moreau, Amanda L. Evans, Meera Arumugam, Guénaëlle Bouët Chalon, Eleonora Vriend, Natalia Davidenko, Cedric Ghevaert, Serena M. Best, Ruth E. Cameron
Published in: Biomaterials, Issue 182, 2018, Page(s) 135-144, ISSN 0142-9612
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.biomaterials.2018.08.019

Mechanisms of platelet release: in vivo studies and in vitro modeling

Author(s): Vittorio Abbonante, Christian A. Di Buduo, Alessandro Malara, Pierre-Alexandre Laurent, Alessandra Balduini
Published in: Platelets, 2020, Page(s) 1-7, ISSN 0953-7104
Publisher: Taylor & Francis
DOI: 10.1080/09537104.2020.1774532

In vitro-derived platelets: the challenges we will have to face to assess quality and safety

Author(s): S. Mookerjee, H. R. Foster, A. K Waller, C. J. Ghevaert
Published in: Platelets, 2020, Page(s) 1-7, ISSN 0953-7104
Publisher: Taylor & Francis
DOI: 10.1080/09537104.2020.1769051

Disrupted filamin A/αIIbβ3 interaction induces macrothrombocytopenia by increasing RhoA activity

Author(s): Alessandro Donada, Nathalie Balayn, Dominika Sliwa, Larissa Lordier, Valentina Ceglia, Francesco Baschieri, Cyril Goizet, Rémi Favier, Lucie Tosca, Gérard Tachdjian, Cecile V. Denis, Isabelle Plo, William Vainchenker, Najet Debili, Jean-Philippe Rosa, Marijke Bryckaert, Hana Raslova
Published in: Blood, Issue 133/16, 2019, Page(s) 1778-1788, ISSN 0006-4971
Publisher: American Society of Hematology
DOI: 10.1182/blood-2018-07-861427

Miniaturized 3D bone marrow tissue model to assess response to Thrombopoietin-receptor agonists in patients

Author(s): Christian A Di Buduo, Pierre-Alexandre Laurent, Carlo Zaninetti, Larissa Lordier, Paolo M Soprano, Aikaterini Ntai, Serena Barozzi, Alberto La Spada, Ida Biunno, Hana Raslova, James B Bussel, David L Kaplan, Carlo L Balduini, Alessandro Pecci, Alessandra Balduini
Published in: eLife, Issue 10, 2021, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.58775

Pathogenetic and clinical study of a patient with thrombocytopenia due to the p.E527K gain-of-function variant of SRC

Author(s): Serena Barozzi, Christian A. Di Buduo, Caterina Marconi, Valeria Bozzi, Marco Seri, Francesca Romano, Alessandra Balduini, Alessandro Pecci
Published in: Haematologica, Issue 106/3, 2021, Page(s) 918-922, ISSN 0390-6078
Publisher: Ferrata Storti Foundation
DOI: 10.3324/haematol.2020.268516

New Challenges for Biobanks: Accreditation to the New ISO 20387:2018 Standard Specific for Biobanks

Author(s): Pasquale De Blasio, Ida Biunno
Published in: BioTech, Issue 10/3, 2021, Page(s) 13, ISSN 2673-6284
Publisher: MDPI
DOI: 10.3390/biotech10030013

Induced pluripotent stem cells and hematological malignancies: A powerful tool for disease modeling and drug development

Author(s): A. Donada, F. Basso-Valentina, B. Arkoun, B. Monte-Mor, I. Plo, H. Raslova
Published in: Stem Cell Research, Issue 49, 2020, Page(s) 102060, ISSN 1873-5061
Publisher: Elsevier
DOI: 10.1016/j.scr.2020.102060

Conference proceedings (7)

Thyroid hormones and analogues promote the acute release of platelets from megakaryocytes: from blood donor biology to the production of platelets in vitro

Author(s): Foster H, Herbert N, Waller A, Di Buduo C, Fang J, Schmidt A, Howard D, Taimoor M, Moreau T, Meuller A, Evans A, Biswas R, Turro E, Fischer R, Wilcox D, Hoffmeister K, Balduini A, Ghevaert C
Published in: Research and Practice in Thrombosis and Haemostasis, 2022
Publisher: Wiley online library

Illustrated State-of-the-Art Capsules of the ISTH 2022 Congress - Megakaryocytes and different thrombopoietic environments

Author(s): Balduini A
Published in: Research and Practice in Thrombosis and Haemostasis, 2022
Publisher: Wiley online library
DOI: 10.1002/rth2.12747

Stimulation of the ssRNA virus immune receptor, Toll-like receptor 7, on megakaryocytes increases thrombopoesis

Author(s): A. Waller, W. Lau, H. Foster, A. Evans, J. Tregoning, A. Poole, A. Davidson, C. Ghevaert, J. Patterson, M. Lawrence, A. Mueller
Published in: Research and Practice in Thrombosis and Haemostasis, 2022
Publisher: Wiley online library

Production of platelets in vitro in functionalised 3-dimensional scaffolds mimicking the bone marrow niche

Author(s): Foster H, Colzani M, Bouet Chalon G, Howard D, Di Buduo C, Muller-Sienerth N, Waller A, Sun Y, Shepherd J, Evans A, Soprano P, Parsons M, Ying Sims Y, Turro E, Maguire P, Best S, Cameron R, Balduini A, Wright G, Ghevaert C
Published in: Research and Practice in Thrombosis and Haemostasis, 2022
Publisher: Wiley online library

Three-D Models for Megakaryopoiesis and Platelet Production

Author(s): Balduini A
Published in: Blood, 2019, Page(s) 134 (Supplement_1): SCI-36
Publisher: American Society of Hematology
DOI: 10.1182/blood-2019-121076

Multi-channel silk sponge for bio-engineering the human bone marrow environment: a new tool for functional platelet production ex vivo

Author(s): Laurent PA, Tozzi L, Di Buduo CA, Muc X, Massaro A, Brethertonc R, Kaplan DL, Balduini A.
Published in: 5 th Tissue Engineering and Regenerative Medicine International Society (TERMIS)-WORLD CONGRESS, 2018
Publisher: TERMIS-WC

Supercharged platelets as a novel therapy for reducing blood loss post-cardiac surgery

Author(s): A. Crosby, S. Mookerjee, A. Waller, H. Foster, L. Du, D. Wilcox, C. Ghevaert
Published in: Research and Practice in Thrombosis and Haemostasis, 2022
Publisher: Wiley online library

Other (1)

ANKRD26 is a new regulator of type I cytokine receptor signaling in normal and pathological hematopoiesis

Author(s): Basso-Valentina F, Donada A, Manchev VT, Lisetto M, Balayn N, Martin JE, Muller D, Marin Oyarzun CP, Duparc H, Arkoun B, Cumin A, Faivre L, Droin N, Biunno I, Balduini A, Debili N, Antony-Debré I, Marty C, Vainchenker W, Plo I, Favier R, Raslova H
Published in: bioRxiv, 2022
Publisher: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.09.01.506160

Intellectual Property Rights

Patent (3)

SILK FIBROIN AND RELATED USE FOR 3D BIOPRINTING

Application/Publication number: 20 22051635
Date: 2022-02-24
Applicant(s): UNIVERSITA DEGLI STUDI DI PAVIA

SYSTEMS AND METHODS FOR ASSESSING PATIENT-SPECIFIC RESPONSE TO THROMBOPOIETIN-RECEPTOR AGONISTS

Application/Publication number: 20 20063639
Date: 2020-12-07
Applicant(s): UNIVERSITA DEGLI STUDI DI PAVIA

SILK FIBROIN AND RELATED USE FOR 3D BIOPRINTING

Application/Publication number: 20 22051635
Date: 2022-02-24
Applicant(s): BICO GROUP AB