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Genetically engineered human pluripotent stem cells, functionalized silk-fibroin platforms and bio-inks: a novel solution for large-scale ex-vivo platelet production, transfusion and drug research

Deliverables

Scientific papers_24

Summary report of the published scientific papers

Targeted stakeholders identified and informed about the project_36

Links to stakeholders as well as experts on Transfusional Medicine and experts on Bioengineering will be established online and by distributing leaflets, poster and press releases

Scientific papers_12

Summary report of the published scientific papers

Bioengineering a 3D silk platform for large-scale platelet production

Bioengineering the first 3D chemically functionalized silk platform for large-scale production of human platelets

Targeted stakeholders identified and informed about the project_12

Links to stakeholders as well as experts on Transfusional Medicine and experts on Bioengineering will be established online and by distributing leaflets, poster and press releases

Workshops_12

Summary report of the performed workshops and training sessions

Targeted stakeholders identified and informed about the project_24

Links to stakeholders as well as experts on Transfusional Medicine and experts on Bioengineering will be established online and by distributing leaflets, poster and press releases

Workshops_36

Summary report of the performed workshops and training sessions

Obtaining a high efficient hPSC lines for producing human platelets for transfusion

A three-level bio-banking system will be applied for the first high efficient hPSC lines

Workshops_24

Summary report of the performed workshops and training sessions

Public Project website

Public Project website and logo will be developed and continuously updated

Project video targeting a wide public audience

Project video targeting a wide public audience will be developed

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Publications

Role of Rho-GTPases in megakaryopoiesis

Author(s): William Vainchenker, Brahim Arkoun, Francesca Basso-Valentina, Larissa Lordier, Najet Debili, Hana Raslova
Published in: Small GTPases, 2021, Page(s) 1-17, ISSN 2154-1248
Publisher: Landes Bioscience
DOI: 10.1080/21541248.2021.1885134

Latest culture techniques: cracking the secrets of bone marrow to mass-produce erythrocytes and platelets <i>ex vivo

Author(s): Christian A. Di Buduo, Alicia Aguilar, Paolo M. Soprano, Alberto Bocconi, Carolina P. Miguel, Giovanna Mantica, Alessandra Balduini
Published in: Haematologica, 2020, ISSN 0390-6078
Publisher: Ferrata Storti Foundation
DOI: 10.3324/haematol.2020.262485

Novel variants in GALE cause syndromic macrothrombocytopenia by disrupting glycosylation and thrombopoiesis

Author(s): Marín-Quilez A, Di Buduo CA, Díaz-Ajenjo L, Abbonante V, Vuelta E, Soprano PMM, Miguel-García C, Santos-Mínguez S, Serramito-Gómez I, Ruiz-Sala P, Peñarrubia MJ, Pardal E, Hernández-Rivas JM, González-Porras JR, García-Tuñón I, Benito R, Rivera J, Balduini A, Bastida JM
Published in: Blood, 00064971, 2022, ISSN 0006-4971
Publisher: American Society of Hematology
DOI: 10.1182/blood.2022016995

Multi-channel silk sponge mimicking bone marrow vascular niche for platelet production

Author(s): Lorenzo Tozzi, Pierre-Alexandre Laurent, Christian A. Di Buduo, Xuan Mu, Angelo Massaro, Ross Bretherton, Whitney Stoppel, David L. Kaplan, Alessandra Balduini
Published in: Biomaterials, 178, 2018, Page(s) 122-133, ISSN 0142-9612
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.biomaterials.2018.06.018

Structurally graduated collagen scaffolds applied to the ex vivo generation of platelets from human pluripotent stem cell-derived megakaryocytes: Enhancing production and purity

Author(s): Jennifer H. Shepherd, Daniel Howard, Amie K. Waller, Holly Rebecca Foster, Annett Mueller, Thomas Moreau, Amanda L. Evans, Meera Arumugam, Guénaëlle Bouët Chalon, Eleonora Vriend, Natalia Davidenko, Cedric Ghevaert, Serena M. Best, Ruth E. Cameron
Published in: Biomaterials, 182, 2018, Page(s) 135-144, ISSN 0142-9612
Publisher: Pergamon Press Ltd.
DOI: 10.1016/j.biomaterials.2018.08.019

Mechanisms of platelet release: in vivo studies and in vitro modeling

Author(s): Vittorio Abbonante, Christian A. Di Buduo, Alessandro Malara, Pierre-Alexandre Laurent, Alessandra Balduini
Published in: Platelets, 2020, Page(s) 1-7, ISSN 0953-7104
Publisher: Taylor & Francis
DOI: 10.1080/09537104.2020.1774532

In vitro-derived platelets: the challenges we will have to face to assess quality and safety

Author(s): S. Mookerjee, H. R. Foster, A. K Waller, C. J. Ghevaert
Published in: Platelets, 2020, Page(s) 1-7, ISSN 0953-7104
Publisher: Taylor & Francis
DOI: 10.1080/09537104.2020.1769051

Disrupted filamin A/αIIbβ3 interaction induces macrothrombocytopenia by increasing RhoA activity

Author(s): Alessandro Donada, Nathalie Balayn, Dominika Sliwa, Larissa Lordier, Valentina Ceglia, Francesco Baschieri, Cyril Goizet, Rémi Favier, Lucie Tosca, Gérard Tachdjian, Cecile V. Denis, Isabelle Plo, William Vainchenker, Najet Debili, Jean-Philippe Rosa, Marijke Bryckaert, Hana Raslova
Published in: Blood, 133/16, 2019, Page(s) 1778-1788, ISSN 0006-4971
Publisher: American Society of Hematology
DOI: 10.1182/blood-2018-07-861427

Miniaturized 3D bone marrow tissue model to assess response to Thrombopoietin-receptor agonists in patients

Author(s): Christian A Di Buduo, Pierre-Alexandre Laurent, Carlo Zaninetti, Larissa Lordier, Paolo M Soprano, Aikaterini Ntai, Serena Barozzi, Alberto La Spada, Ida Biunno, Hana Raslova, James B Bussel, David L Kaplan, Carlo L Balduini, Alessandro Pecci, Alessandra Balduini
Published in: eLife, 10, 2021, ISSN 2050-084X
Publisher: eLife Sciences Publications
DOI: 10.7554/elife.58775

Pathogenetic and clinical study of a patient with thrombocytopenia due to the p.E527K gain-of-function variant of SRC

Author(s): Serena Barozzi, Christian A. Di Buduo, Caterina Marconi, Valeria Bozzi, Marco Seri, Francesca Romano, Alessandra Balduini, Alessandro Pecci
Published in: Haematologica, 106/3, 2021, Page(s) 918-922, ISSN 0390-6078
Publisher: Ferrata Storti Foundation
DOI: 10.3324/haematol.2020.268516

New Challenges for Biobanks: Accreditation to the New ISO 20387:2018 Standard Specific for Biobanks

Author(s): Pasquale De Blasio, Ida Biunno
Published in: BioTech, 10/3, 2021, Page(s) 13, ISSN 2673-6284
Publisher: MDPI
DOI: 10.3390/biotech10030013

Induced pluripotent stem cells and hematological malignancies: A powerful tool for disease modeling and drug development

Author(s): A. Donada, F. Basso-Valentina, B. Arkoun, B. Monte-Mor, I. Plo, H. Raslova
Published in: Stem Cell Research, 49, 2020, Page(s) 102060, ISSN 1873-5061
Publisher: Elsevier
DOI: 10.1016/j.scr.2020.102060

Thyroid hormones and analogues promote the acute release of platelets from megakaryocytes: from blood donor biology to the production of platelets in vitro

Author(s): Foster H, Herbert N, Waller A, Di Buduo C, Fang J, Schmidt A, Howard D, Taimoor M, Moreau T, Meuller A, Evans A, Biswas R, Turro E, Fischer R, Wilcox D, Hoffmeister K, Balduini A, Ghevaert C
Published in: Research and Practice in Thrombosis and Haemostasis, 2022
Publisher: Wiley online library

Illustrated State-of-the-Art Capsules of the ISTH 2022 Congress - Megakaryocytes and different thrombopoietic environments

Author(s): Balduini A
Published in: Research and Practice in Thrombosis and Haemostasis, 2022
Publisher: Wiley online library
DOI: 10.1002/rth2.12747

Stimulation of the ssRNA virus immune receptor, Toll-like receptor 7, on megakaryocytes increases thrombopoesis

Author(s): A. Waller, W. Lau, H. Foster, A. Evans, J. Tregoning, A. Poole, A. Davidson, C. Ghevaert, J. Patterson, M. Lawrence, A. Mueller
Published in: Research and Practice in Thrombosis and Haemostasis, 2022
Publisher: Wiley online library

Production of platelets in vitro in functionalised 3-dimensional scaffolds mimicking the bone marrow niche

Author(s): Foster H, Colzani M, Bouet Chalon G, Howard D, Di Buduo C, Muller-Sienerth N, Waller A, Sun Y, Shepherd J, Evans A, Soprano P, Parsons M, Ying Sims Y, Turro E, Maguire P, Best S, Cameron R, Balduini A, Wright G, Ghevaert C
Published in: Research and Practice in Thrombosis and Haemostasis, 2022
Publisher: Wiley online library

Three-D Models for Megakaryopoiesis and Platelet Production

Author(s): Balduini A
Published in: Blood, 2019, Page(s) 134 (Supplement_1): SCI-36
Publisher: American Society of Hematology
DOI: 10.1182/blood-2019-121076

Multi-channel silk sponge for bio-engineering the human bone marrow environment: a new tool for functional platelet production ex vivo

Author(s): Laurent PA, Tozzi L, Di Buduo CA, Muc X, Massaro A, Brethertonc R, Kaplan DL, Balduini A.
Published in: 5 th Tissue Engineering and Regenerative Medicine International Society (TERMIS)-WORLD CONGRESS, 2018
Publisher: TERMIS-WC

Novel variants in GALE caused syndromic macrothrombocytopenia disrupting thrombopoiesis and glycosylation

Author(s): Marin-Quilez A, Di Buduo C, Díaz-Ajenjo L, Soprano P, Vuelta E, Serramito-Gómez I, Santos-Mínguez S, Miguel-García C, Tocino-Antonio S, Zamora-Canovas A, Ruiz-Sala P, Peñarrubia M, Pardal E, Hernández-Rivas J, González-Porras J, Benito R, Rivera J, García-Tuñón I, Balduini A, Bastida J.
Published in: Research and Practice in Thrombosis and Haemostasis, 2022
Publisher: Wiley online library

Supercharged platelets as a novel therapy for reducing blood loss post-cardiac surgery

Author(s): A. Crosby, S. Mookerjee, A. Waller, H. Foster, L. Du, D. Wilcox, C. Ghevaert
Published in: Research and Practice in Thrombosis and Haemostasis, 2022
Publisher: Wiley online library