Genetically engineered human pluripotent stem cells, functionalized silk-fibroin platforms and bio-inks: a novel solution for large-scale ex-vivo platelet production, transfusion and drug research

Información del proyecto

SilkFUSION

Identificador del acuerdo de subvención: 767309

DOI

10.3030/767309

Proyecto cerrado

Fecha de la firma de la CE 12 Septiembre 2017

Fecha de inicio 1 Noviembre 2017

Fecha de finalización 31 Octubre 2022

Financiado con arreglo a

• EXCELLENT SCIENCE - Future and Emerging Technologies (FET)

Coste total

€ 2 998 500,01

Aportación de la UE

€ 2 998 500,00

Coordinado por

UNIVERSITA DEGLI STUDI DI PAVIA
Italy

CORDIS proporciona enlaces a los documentos públicos y las publicaciones de los proyectos de los programas marco HORIZONTE.

Los enlaces a los documentos y las publicaciones de los proyectos del Séptimo Programa Marco, así como los enlaces a algunos tipos de resultados específicos, como conjuntos de datos y «software», se obtienen dinámicamente de OpenAIRE .

Resultado final

Documents, reports (14)

Scientific papers_24

Summary report of the published scientific papers

Targeted stakeholders identified and informed about the project_36

Links to stakeholders as well as experts on Transfusional Medicine and experts on Bioengineering will be established online and by distributing leaflets, poster and press releases

Scientific papers_12

Summary report of the published scientific papers

Bioengineering a 3D silk platform for large-scale platelet production

Bioengineering the first 3D chemically functionalized silk platform for large-scale production of human platelets

Scientific papers_36

Summary report of the published scientific papers

Targeted stakeholders identified and informed about the project_12

Links to stakeholders as well as experts on Transfusional Medicine and experts on Bioengineering will be established online and by distributing leaflets, poster and press releases

Workshops_12

Summary report of the performed workshops and training sessions

Targeted stakeholders identified and informed about the project_24

Links to stakeholders as well as experts on Transfusional Medicine and experts on Bioengineering will be established online and by distributing leaflets, poster and press releases

Targeted stakeholders identified and informed about the project_48

Links to stakeholders as well as experts on Transfusional Medicine and experts on Bioengineering will be established online and by distributing leaflets poster and press releases

Workshops_36

Summary report of the performed workshops and training sessions

Obtaining a high efficient hPSC lines for producing human platelets for transfusion

A three-level bio-banking system will be applied for the first high efficient hPSC lines

Workshops_48

Summary report of the performed workshops and training sessions

Workshops_24

Summary report of the performed workshops and training sessions

Scientific papers_48

Summary report of the published scientific papers

Websites, patent fillings, videos etc. (2)

Public Project website

Public Project website and logo will be developed and continuously updated

Project video targeting a wide public audience

Project video targeting a wide public audience will be developed

Publicaciones

Peer reviewed articles (12)

Role of Rho-GTPases in megakaryopoiesis

Autores: William Vainchenker, Brahim Arkoun, Francesca Basso-Valentina, Larissa Lordier, Najet Debili, Hana Raslova
Publicado en: Small GTPases, 2021, Página(s) 1-17, ISSN 2154-1248
Editor: Landes Bioscience
DOI: 10.1080/21541248.2021.1885134

Latest culture techniques: cracking the secrets of bone marrow to mass-produce erythrocytes and platelets <i>ex vivo

Autores: Christian A. Di Buduo, Alicia Aguilar, Paolo M. Soprano, Alberto Bocconi, Carolina P. Miguel, Giovanna Mantica, Alessandra Balduini
Publicado en: Haematologica, 2020, ISSN 0390-6078
Editor: Ferrata Storti Foundation
DOI: 10.3324/haematol.2020.262485

Novel variants in GALE cause syndromic macrothrombocytopenia by disrupting glycosylation and thrombopoiesis

Autores: Marín-Quilez A, Di Buduo CA, Díaz-Ajenjo L, Abbonante V, Vuelta E, Soprano PMM, Miguel-García C, Santos-Mínguez S, Serramito-Gómez I, Ruiz-Sala P, Peñarrubia MJ, Pardal E, Hernández-Rivas JM, González-Porras JR, García-Tuñón I, Benito R, Rivera J, Balduini A, Bastida JM
Publicado en: Blood, 2022, ISSN 0006-4971
Editor: American Society of Hematology
DOI: 10.1182/blood.2022016995

Multi-channel silk sponge mimicking bone marrow vascular niche for platelet production

Autores: Lorenzo Tozzi, Pierre-Alexandre Laurent, Christian A. Di Buduo, Xuan Mu, Angelo Massaro, Ross Bretherton, Whitney Stoppel, David L. Kaplan, Alessandra Balduini
Publicado en: Biomaterials, Edición 178, 2018, Página(s) 122-133, ISSN 0142-9612
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.biomaterials.2018.06.018

Structurally graduated collagen scaffolds applied to the ex vivo generation of platelets from human pluripotent stem cell-derived megakaryocytes: Enhancing production and purity

Autores: Jennifer H. Shepherd, Daniel Howard, Amie K. Waller, Holly Rebecca Foster, Annett Mueller, Thomas Moreau, Amanda L. Evans, Meera Arumugam, Guénaëlle Bouët Chalon, Eleonora Vriend, Natalia Davidenko, Cedric Ghevaert, Serena M. Best, Ruth E. Cameron
Publicado en: Biomaterials, Edición 182, 2018, Página(s) 135-144, ISSN 0142-9612
Editor: Pergamon Press Ltd.
DOI: 10.1016/j.biomaterials.2018.08.019

Mechanisms of platelet release: in vivo studies and in vitro modeling

Autores: Vittorio Abbonante, Christian A. Di Buduo, Alessandro Malara, Pierre-Alexandre Laurent, Alessandra Balduini
Publicado en: Platelets, 2020, Página(s) 1-7, ISSN 0953-7104
Editor: Taylor & Francis
DOI: 10.1080/09537104.2020.1774532

In vitro-derived platelets: the challenges we will have to face to assess quality and safety

Autores: S. Mookerjee, H. R. Foster, A. K Waller, C. J. Ghevaert
Publicado en: Platelets, 2020, Página(s) 1-7, ISSN 0953-7104
Editor: Taylor & Francis
DOI: 10.1080/09537104.2020.1769051

Disrupted filamin A/αIIbβ3 interaction induces macrothrombocytopenia by increasing RhoA activity

Autores: Alessandro Donada, Nathalie Balayn, Dominika Sliwa, Larissa Lordier, Valentina Ceglia, Francesco Baschieri, Cyril Goizet, Rémi Favier, Lucie Tosca, Gérard Tachdjian, Cecile V. Denis, Isabelle Plo, William Vainchenker, Najet Debili, Jean-Philippe Rosa, Marijke Bryckaert, Hana Raslova
Publicado en: Blood, Edición 133/16, 2019, Página(s) 1778-1788, ISSN 0006-4971
Editor: American Society of Hematology
DOI: 10.1182/blood-2018-07-861427

Miniaturized 3D bone marrow tissue model to assess response to Thrombopoietin-receptor agonists in patients

Autores: Christian A Di Buduo, Pierre-Alexandre Laurent, Carlo Zaninetti, Larissa Lordier, Paolo M Soprano, Aikaterini Ntai, Serena Barozzi, Alberto La Spada, Ida Biunno, Hana Raslova, James B Bussel, David L Kaplan, Carlo L Balduini, Alessandro Pecci, Alessandra Balduini
Publicado en: eLife, Edición 10, 2021, ISSN 2050-084X
Editor: eLife Sciences Publications
DOI: 10.7554/elife.58775

Pathogenetic and clinical study of a patient with thrombocytopenia due to the p.E527K gain-of-function variant of SRC

Autores: Serena Barozzi, Christian A. Di Buduo, Caterina Marconi, Valeria Bozzi, Marco Seri, Francesca Romano, Alessandra Balduini, Alessandro Pecci
Publicado en: Haematologica, Edición 106/3, 2021, Página(s) 918-922, ISSN 0390-6078
Editor: Ferrata Storti Foundation
DOI: 10.3324/haematol.2020.268516

New Challenges for Biobanks: Accreditation to the New ISO 20387:2018 Standard Specific for Biobanks

Autores: Pasquale De Blasio, Ida Biunno
Publicado en: BioTech, Edición 10/3, 2021, Página(s) 13, ISSN 2673-6284
Editor: MDPI
DOI: 10.3390/biotech10030013

Induced pluripotent stem cells and hematological malignancies: A powerful tool for disease modeling and drug development

Autores: A. Donada, F. Basso-Valentina, B. Arkoun, B. Monte-Mor, I. Plo, H. Raslova
Publicado en: Stem Cell Research, Edición 49, 2020, Página(s) 102060, ISSN 1873-5061
Editor: Elsevier
DOI: 10.1016/j.scr.2020.102060

Conference proceedings (7)

Thyroid hormones and analogues promote the acute release of platelets from megakaryocytes: from blood donor biology to the production of platelets in vitro

Autores: Foster H, Herbert N, Waller A, Di Buduo C, Fang J, Schmidt A, Howard D, Taimoor M, Moreau T, Meuller A, Evans A, Biswas R, Turro E, Fischer R, Wilcox D, Hoffmeister K, Balduini A, Ghevaert C
Publicado en: Research and Practice in Thrombosis and Haemostasis, 2022
Editor: Wiley online library

Illustrated State-of-the-Art Capsules of the ISTH 2022 Congress - Megakaryocytes and different thrombopoietic environments

Autores: Balduini A
Publicado en: Research and Practice in Thrombosis and Haemostasis, 2022
Editor: Wiley online library
DOI: 10.1002/rth2.12747

Stimulation of the ssRNA virus immune receptor, Toll-like receptor 7, on megakaryocytes increases thrombopoesis

Autores: A. Waller, W. Lau, H. Foster, A. Evans, J. Tregoning, A. Poole, A. Davidson, C. Ghevaert, J. Patterson, M. Lawrence, A. Mueller
Publicado en: Research and Practice in Thrombosis and Haemostasis, 2022
Editor: Wiley online library

Production of platelets in vitro in functionalised 3-dimensional scaffolds mimicking the bone marrow niche

Autores: Foster H, Colzani M, Bouet Chalon G, Howard D, Di Buduo C, Muller-Sienerth N, Waller A, Sun Y, Shepherd J, Evans A, Soprano P, Parsons M, Ying Sims Y, Turro E, Maguire P, Best S, Cameron R, Balduini A, Wright G, Ghevaert C
Publicado en: Research and Practice in Thrombosis and Haemostasis, 2022
Editor: Wiley online library

Three-D Models for Megakaryopoiesis and Platelet Production

Autores: Balduini A
Publicado en: Blood, 2019, Página(s) 134 (Supplement_1): SCI-36
Editor: American Society of Hematology
DOI: 10.1182/blood-2019-121076

Multi-channel silk sponge for bio-engineering the human bone marrow environment: a new tool for functional platelet production ex vivo

Autores: Laurent PA, Tozzi L, Di Buduo CA, Muc X, Massaro A, Brethertonc R, Kaplan DL, Balduini A.
Publicado en: 5 th Tissue Engineering and Regenerative Medicine International Society (TERMIS)-WORLD CONGRESS, 2018
Editor: TERMIS-WC

Supercharged platelets as a novel therapy for reducing blood loss post-cardiac surgery

Autores: A. Crosby, S. Mookerjee, A. Waller, H. Foster, L. Du, D. Wilcox, C. Ghevaert
Publicado en: Research and Practice in Thrombosis and Haemostasis, 2022
Editor: Wiley online library

Other (1)

ANKRD26 is a new regulator of type I cytokine receptor signaling in normal and pathological hematopoiesis

Autores: Basso-Valentina F, Donada A, Manchev VT, Lisetto M, Balayn N, Martin JE, Muller D, Marin Oyarzun CP, Duparc H, Arkoun B, Cumin A, Faivre L, Droin N, Biunno I, Balduini A, Debili N, Antony-Debré I, Marty C, Vainchenker W, Plo I, Favier R, Raslova H
Publicado en: bioRxiv, 2022
Editor: Cold Spring Harbor Laboratory
DOI: 10.1101/2022.09.01.506160

Derechos de propiedad intelectual

Patent (3)

SILK FIBROIN AND RELATED USE FOR 3D BIOPRINTING

Número de solicitud/publicación: 20 22051635
Fecha: 2022-02-24
Solicitante(s): UNIVERSITA DEGLI STUDI DI PAVIA

SYSTEMS AND METHODS FOR ASSESSING PATIENT-SPECIFIC RESPONSE TO THROMBOPOIETIN-RECEPTOR AGONISTS

Número de solicitud/publicación: 20 20063639
Fecha: 2020-12-07
Solicitante(s): UNIVERSITA DEGLI STUDI DI PAVIA

SILK FIBROIN AND RELATED USE FOR 3D BIOPRINTING

Número de solicitud/publicación: 20 22051635
Fecha: 2022-02-24
Solicitante(s): BICO GROUP AB